Restriction map for
pF1KE0238
(Circular) (Six-base) MAPSORT of: pF1KE0238.seq Check: 6650 from: 1 to: 3599
pF1KE0238 3599 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 637 637
Size: 3599
Acc65I G'GTAC_C
Cuts at: 615 615
Size: 3599
AclI AA'CG_TT
Cuts at: 3439 3439
Size: 3599
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1424 1856 2603 2871 1424
Size: 432 747 268 2152
Fragments arranged by size:
2152 747 432 268
AflIII A'CryG_T
Cuts at: 951 2055 3032 951
Size: 1104 977 1518
Fragments arranged by size:
1518 1104 977
AgeI A'CCGG_T
Cuts at: 1987 1987
Size: 3599
AlwNI CAG_nnn'CTG
Cuts at: 904 2471 904
Size: 1567 2032
ApaLI G'TGCA_C
Cuts at: 2369 2369
Size: 3599
ApoI r'AATT_y
Cuts at: 603 2952 3208 603
Size: 2349 256 994
Fragments arranged by size:
2349 994 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3599
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3599
AvaI C'yCGr_G
Cuts at: 619 619
Size: 3599
BamHI G'GATC_C
Cuts at: 560 624 560
Size: 64 3535
BanI G'GyrC_C
Cuts at: 615 1029 1277 1312 615
Size: 414 248 35 2902
Fragments arranged by size:
2902 414 248 35
BanII G_rGCy'C
Cuts at: 613 1643 613
Size: 1030 2569
BbeI G_GCGC'C
Cuts at: 1033 1281 1033
Size: 248 3351
BciVI GTATCCnnnnn_n'
Cuts at: 1497 2258 3599 1497
Size: 761 1341 1497
Fragments arranged by size:
1497 1341 761
BclI T'GATC_A
Cuts at: 1119 1119
Size: 3599
BglII A'GATC_T
Cuts at: 1114 3151 1114
Size: 2037 1562
BlpI GC'TnA_GC
Cuts at: 711 711
Size: 3599
Bme1580I G_kGCm'C
Cuts at: 1224 1317 2373 1224
Size: 93 1056 2450
Fragments arranged by size:
2450 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 186 811 892 1227 3371 186
Size: 625 81 335 2144 414
Fragments arranged by size:
2144 625 414 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1966 1966
Size: 3599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 257
Size: 3599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 1654 2146 2444 775
Size: 879 492 298 1930
Fragments arranged by size:
1930 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 134 3303 134
Size: 3169 430
BsaAI yAC'GTr
Cuts at: 189 952 189
Size: 763 2836
BsaBI GATnn'nnATC
Cuts at: 6 1113 3150 6
Size: 1107 2037 455
Fragments arranged by size:
2037 1107 455
BsaHI Gr'CG_yC
Cuts at: 476 1030 1278 1980 3476 476
Size: 554 248 702 1496 599
Fragments arranged by size:
1496 702 599 554 248
BsaWI w'CCGG_w
Cuts at: 788 1309 1987 2261 2408 2819 788
Size: 521 678 274 147 411 1568
Fragments arranged by size:
1568 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 412 412
Size: 3599
BseYI C'CCAG_C
Cuts at: 313 1024 1782 2359 2855 313
Size: 711 758 577 496 1057
Fragments arranged by size:
1057 758 711 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 524
Size: 3599
BsiEI CG_ry'CG
Cuts at: 104 1141 1187 2395 104
Size: 1037 46 1208 1308
Fragments arranged by size:
1308 1208 1037 46
BsiHKAI G_wGCw'C
Cuts at: 452 613 1391 1581 2373 452
Size: 161 778 190 792 1678
Fragments arranged by size:
1678 792 778 190 161
BsmBI CGTCTCn'nnnn_
Cuts at: 483 483
Size: 3599
Bsp1286I G_dGCh'C
Cuts at: 452 613 1224 1317 1391 1581 1643 2373 452
Size: 161 611 93 74 190 62 730 1678
Fragments arranged by size:
1678 730 611 190 161 93 74 62
BspEI T'CCGG_A
Cuts at: 2819 2819
Size: 3599
BspHI T'CATG_A
Cuts at: 2775 2775
Size: 3599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 649 863 1165 1546 649
Size: 214 302 381 2702
Fragments arranged by size:
2702 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1891 1945 3429 3594 50
Size: 1841 54 1484 165 55
Fragments arranged by size:
1841 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1511 1511
Size: 3599
BsrFI r'CCGG_y
Cuts at: 1597 1778 1987 1597
Size: 181 209 3209
Fragments arranged by size:
3209 209 181
BssSI C'ACGA_G
Cuts at: 1870 2228 1870
Size: 358 3241
BstAPI GCAn_nnn'nTGC
Cuts at: 417 976 417
Size: 559 3040
BstBI TT'CG_AA
Cuts at: 1960 1960
Size: 3599
BstXI CCAn_nnnn'nTGG
Cuts at: 114 189 499 114
Size: 75 310 3214
Fragments arranged by size:
3214 310 75
BstYI r'GATC_y
Cuts at: 1 560 624 1091 1114 1449 1695 2707 2793
Size: 559 64 467 23 335 246 1012 86
Cuts at: 2793 2805 3151 1
Size: 12 346 449
Fragments arranged by size:
1012 559 467 449 346 335 246 86
64 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3599
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1549 1730 1893 1549
Size: 181 163 3255
Fragments arranged by size:
3255 181 163
BtsI GCAGTG_nn'
Cuts at: 364 492 842 364
Size: 128 350 3121
Fragments arranged by size:
3121 350 128
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 536 571 536
Size: 35 3564
DraI TTT'AAA
Cuts at: 598 598
Size: 3599
DraIII CAC_nnn'GTG
Cuts at: 456 456
Size: 3599
DrdI GACnn_nn'nnGTC
Cuts at: 1305 2163 1305
Size: 858 2741
EaeI y'GGCC_r
Cuts at: 116 248 569 1184 1358 1749 1776 3121 116
Size: 132 321 615 174 391 27 1345 594
Fragments arranged by size:
1345 615 594 391 321 174 132 27
EagI C'GGCC_G
Cuts at: 1184 1184
Size: 3599
EarI CTCTTCn'nnn_
Cuts at: 1622 1832 1622
Size: 210 3389
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1807 2117 2263 3409 1807
Size: 310 146 1146 1997
Fragments arranged by size:
1997 1146 310 146
EcoICRI GAG'CTC
Cuts at: 611 611
Size: 3599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 1424 1856 2603 2871 257
Size: 1167 432 747 268 985
Fragments arranged by size:
1167 985 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 738 738
Size: 3599
EcoRI G'AATT_C
Cuts at: 603 603
Size: 3599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1618 1618
Size: 3599
FspI TGC'GCA
Cuts at: 1380 1380
Size: 3599
HaeII r_GCGC'y
Cuts at: 1033 1281 2303 3282 1033
Size: 248 1022 979 1350
Fragments arranged by size:
1350 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1441 1471 1473 1503 1441
Size: 30 2 30 3537
Fragments arranged by size:
3537 30 30 2
HincII GTy'rAC
Cuts at: 638 638
Size: 3599
KasI G'GCGC_C
Cuts at: 1029 1277 1029
Size: 248 3351
KpnI G_GTAC'C
Cuts at: 619 619
Size: 3599
MluI A'CGCG_T
Cuts at: 3032 3032
Size: 3599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 398 2270 2454 398
Size: 1872 184 1543
Fragments arranged by size:
1872 1543 184
MscI TGG'CCA
Cuts at: 118 571 1360 118
Size: 453 789 2357
Fragments arranged by size:
2357 789 453
MslI CAynn'nnrTG
Cuts at: 187 1588 1715 187
Size: 1401 127 2071
Fragments arranged by size:
2071 1401 127
MspA1I CmG'CkG
Cuts at: 710 1024 1384 2397 2642 710
Size: 314 360 1013 245 1667
Fragments arranged by size:
1667 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1780 1780
Size: 3599
NarI GG'CG_CC
Cuts at: 1030 1278 1030
Size: 248 3351
NcoI C'CATG_G
Cuts at: 107 107
Size: 3599
NgoMIV G'CCGG_C
Cuts at: 1778 1778
Size: 3599
NspI r_CATG'y
Cuts at: 652 1152 2059 652
Size: 500 907 2192
Fragments arranged by size:
2192 907 500
PciI A'CATG_T
Cuts at: 2055 2055
Size: 3599
PmeI GTTT'AAAC
Cuts at: 598 598
Size: 3599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2770 2738
Size: 32 3567
PstI C_TGCA'G
Cuts at: 646 1331 646
Size: 685 2914
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3599
PvuII CAG'CTG
Cuts at: 1024 1384 1024
Size: 360 3239
RsrII CG'GwC_CG
Cuts at: 1794 1794
Size: 3599
SacI G_AGCT'C
Cuts at: 613 613
Size: 3599
SalI G'TCGA_C
Cuts at: 636 636
Size: 3599
SapI GCTCTTCn'nnn_
Cuts at: 1622 1832 1622
Size: 210 3389
SbfI CC_TGCA'GG
Cuts at: 646 646
Size: 3599
ScaI AGT'ACT
Cuts at: 3116 3116
Size: 3599
SexAI A'CCwGG_T
Cuts at: 491 491
Size: 3599
SfcI C'TryA_G
Cuts at: 33 327 642 1327 2320 2511 33
Size: 294 315 685 993 191 1121
Fragments arranged by size:
1121 993 685 315 294 191
SfoI GGC'GCC
Cuts at: 1031 1279 1031
Size: 248 3351
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3599
SmaI CCC'GGG
Cuts at: 621 621
Size: 3599
SmlI C'TyrA_G
Cuts at: 754 1669 2161 2423 754
Size: 915 492 262 1930
Fragments arranged by size:
1930 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3599
SphI G_CATG'C
Cuts at: 652 1152 652
Size: 500 3099
StuI AGG'CCT
Cuts at: 533 533
Size: 3599
StyI C'CwwG_G
Cuts at: 107 733 1087 107
Size: 626 354 2619
Fragments arranged by size:
2619 626 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 144 1182 1315 1774 1983 144
Size: 1038 133 459 209 1760
Fragments arranged by size:
1760 1038 459 209 133
TatI w'GTAC_w
Cuts at: 3114 3114
Size: 3599
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3567
Tth111I GACn'n_nGTC
Cuts at: 493 1396 493
Size: 903 2696
XbaI T'CTAG_A
Cuts at: 630 630
Size: 3599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 469 1094 469
Size: 625 2974
XmaI C'CCGG_G
Cuts at: 619 619
Size: 3599
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI