Restriction map for pF1KE0186
##################################
# NO CUT ENZYMES (  182 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BmyI        BpmI        BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HhaI        Hin1I       HinP1I      HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TspRI       
Tth111I     XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AclI           38  AACGTT               AA^CGTT
AvaII         427  GGACC                G^G[AT]CC
BbsI          203  GTCTTC               GAAGAC(2/6)
BfaI          175  CTAG                 C^TAG
BlpI          576  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      576  GCTGAGC              GC^T[ACGT]AGC
BpuAI         203  GTCTTC               GAAGAC(2/6)
BseRI         214  GAGGAG               GAGGAG(10/8)
BsgI           47  GTGCAG               GTGCAG(16/14)
BsmBI         635  CGTCTC               CGTCTC(1/5)
BsrI          592  CCAGT                ACTGG(1/-1)
Cac8I         343  GCAAGC               GC[ACGT]^[ACGT]GC
CelII         576  GCTGAGC              GC^T[ACGT]AGC
DrdI          194  GACAGACATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeIII        590  GGCC                 GG^CC
HgaI          268  GCGTC                GACGC(5/10)
HincII        556  GTCAAC               GT[CT]^[GA]AC
HindII        556  GTCAAC               GT[CT]^[GA]AC
HindIII       345  AAGCTT               A^AGCTT
HphI           79  GGTGA                GGTGA(8/7)
MaeI          175  CTAG                 C^TAG
MaeIII        168  GTCAC                ^GT[ACGT]AC
MwoI          122  GCTACATGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          381  CATATG               CA^TATG
NlaIV         426  GGGACC               GG[ACGT]^[ACGT]CC
PleI          516  GACTC                GAGTC(4/5)
Psp1406I       38  AACGTT               AA^CGTT
PstI          260  CTGCAG               CTGCA^G
TaqI          224  TCGA                 T^CGA
TfiI          606  GAATC                G^A[AT]TC
Tsp45I        168  GTCAC                ^GT[GC]AC
TthHB8I       224  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          328  GTAC                 GT^AC
              484  GTAC                
AflIII        125  ACATGT               A^C[GA][CT]GT
              199  ACATGT              
Asp700I       388  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              504  GAATGAATTC          
BbvI          262  GCAGC                GCAGC(8/12)
              265  GCAGC               
BsaI           96  GAGACC               GGTCTC(1/5)
              360  GAGACC              
BsiYI         460  CCAATACTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              592  CCAGTTGTTGG         
BslI          460  CCAATACTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              592  CCAGTTGTTGG         
BspHI         396  TCATGA               T^CATGA
              512  TCATGA              
BspLU11I      125  ACATGT               A^CATGT
              199  ACATGT              
Cfr13I        427  GGACC                G^G[ACGT]CC
              589  GGGCC               
Fnu4HI        262  GCAGC                GC^[ACGT]GC
              265  GCAGC               
HinfI         516  GACTC                G^A[ACGT]TC
              606  GAATC               
ItaI          262  GCAGC                GC^[ACGT]GC
              265  GCAGC               
MaeII          39  ACGT                 A^CGT
              634  ACGT                
NspI          125  ACATGT               [GA]CATG^[CT]
              199  ACATGT              
PflMI         460  CCAATACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              592  CCAGTTGTTGG         
RcaI          396  TCATGA               T^CATGA
              512  TCATGA              
RsaI          328  GTAC                 GT^AC
              484  GTAC                
Sau96I        427  GGACC                G^G[ACGT]CC
              589  GGGCC               
SfaNI         411  GATGC                GCATC(5/9)
              181  GCATC               
TseI          262  GCAGC                G^C[AT]GC
              265  GCAGC               
Van91I        460  CCAATACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              592  CCAGTTGTTGG         
XmnI          388  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              504  GAATGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AcsI        AluI        ApoI        BsaJI       BsmAI       
BsmFI       BstF5I      DdeI        DsaI        EcoRI       
EcoT14I     FokI        MboII       MnlI        MseI        
NcoI        NlaIII      SfcI        StyI        Tru9I       
Tsp509I     
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