Restriction map for pF1KE0183
 (Linear) (Six-base) MAPSORT of: KIEE0183.seq  Check: 3082  from: 1  to: 966



KIEE0183.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AflIII A'CryG_T

Cuts at:      0     942     966 
   Size:        942      24
 
AleI CACnn'nnGTG

Cuts at:      0     843     966 
   Size:        843     123
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     521     553     966 
   Size:        521      32     413

  Fragments arranged by size: 

                 521     413      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     715     966 
   Size:        715     251
 
ApaLI G'TGCA_C

Cuts at:      0     791     966 
   Size:        791     175
 
AseI AT'TA_AT

Cuts at:      0     572     583     966 
   Size:        572      11     383

  Fragments arranged by size: 

                 572     383      11
 
AvaI C'yCGr_G

Cuts at:      0     130     966 
   Size:        130     836
 
BanI G'GyrC_C

Cuts at:      0     469     966 
   Size:        469     497
 
BfrBI ATG'CAT

Cuts at:      0     499     966 
   Size:        499     467
 
Bme1580I G_kGCm'C

Cuts at:      0     795     855     966 
   Size:        795      60     111

  Fragments arranged by size: 

                 795     111      60
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     966 
   Size:         26     940
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     886     916     966 
   Size:        886      30      50

  Fragments arranged by size: 

                 886      50      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     795     966 
   Size:        795     171
 
BsmI GAATG_Cn'

Cuts at:      0     611     966 
   Size:        611     355
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     407     966 
   Size:        407     559
 
Bsp1286I G_dGCh'C

Cuts at:      0     795     855     966 
   Size:        795      60     111

  Fragments arranged by size: 

                 795     111      60
 
BsrFI r'CCGG_y

Cuts at:      0     342     966 
   Size:        342     624
 
BstYI r'GATC_y

Cuts at:      0     914     966 
   Size:        914      52
 
BtgI C'CryG_G

Cuts at:      0     781     966 
   Size:        781     185
 
BtsI GCAGTG_nn'

Cuts at:      0     367     966 
   Size:        367     599
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     390     425     966 
   Size:        390      35     541

  Fragments arranged by size: 

                 541     390      35
 
EaeI y'GGCC_r

Cuts at:      0     722     966 
   Size:        722     244
 
EarI CTCTTCn'nnn_

Cuts at:      0     766     966 
   Size:        766     200
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     966 
   Size:         26     940
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     780     966 
   Size:        780     186
 
HincII GTy'rAC

Cuts at:      0     116     966 
   Size:        116     850
 
HindIII A'AGCT_T

Cuts at:      0     888     966 
   Size:        888      78
 
HpaI GTT'AAC

Cuts at:      0     116     966 
   Size:        116     850
 
MluI A'CGCG_T

Cuts at:      0     942     966 
   Size:        942      24
 
MscI TGG'CCA

Cuts at:      0     724     966 
   Size:        724     242
 
MslI CAynn'nnrTG

Cuts at:      0     298     843     880     966 
   Size:        298     545      37      86

  Fragments arranged by size: 

                 545     298      86      37
 
NaeI GCC'GGC

Cuts at:      0     344     966 
   Size:        344     622
 
NcoI C'CATG_G

Cuts at:      0     781     966 
   Size:        781     185
 
NgoMIV G'CCGG_C

Cuts at:      0     342     966 
   Size:        342     624
 
NsiI A_TGCA'T

Cuts at:      0     501     966 
   Size:        501     465
 
NspI r_CATG'y

Cuts at:      0     514     966 
   Size:        514     452
 
PfoI T'CCnGG_A

Cuts at:      0     694     966 
   Size:        694     272
 
SmlI C'TyrA_G

Cuts at:      0     130     966 
   Size:        130     836
 
SspI AAT'ATT

Cuts at:      0     576     966 
   Size:        576     390
 
StyI C'CwwG_G

Cuts at:      0     781     966 
   Size:        781     185
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     810     966 
   Size:        810     156
 
TatI w'GTAC_w

Cuts at:      0     150     215     947     966 
   Size:        150      65     732      19

  Fragments arranged by size: 

                 732     150      65      19
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     702     966 
   Size:        702     264
 
XhoI C'TCGA_G

Cuts at:      0     130     966 
   Size:        130     836
 
XmnI GAAnn'nnTTC

Cuts at:      0     775     966 
   Size:        775     191
 

 Enzymes that do cut:

   AflIII     AleI     AloI    AlwNI    ApaLI     AseI     AvaI     BanI
    BfrBI Bme1580I     BpmI    BsaXI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BsrFI    BstYI     BtgI     BtsI    CspCI     EaeI     EarI  Eco57MI
     FalI   HincII  HindIII     HpaI     MluI     MscI     MslI     NaeI
     NcoI   NgoMIV     NsiI     NspI     PfoI     SmlI     SspI     StyI
    TaqII     TatI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AlfI     ApaI     ApoI     AscI    AsiSI    AvrII
     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI    BsiWI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I     KasI     KpnI     MfeI     MmeI   MspA1I
     NarI     NdeI     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI     TstI  Tth111I     XbaI     XmaI     ZraI
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