Restriction map for pF1KE1842
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaII       AvrII       BalI        
BamHI       BbrPI       BbsI        BbvCI       BciVI       
BfaI        BfrI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiWI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       Bst1107I    BstBI       BstYI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoNI       EcoRI       EcoRV       EcoT22I     HincII      
HindII      HindIII     HpaI        KpnI        MaeI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MunI        NdeI        
NheI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SapI        SbfI        ScaI        SexAI       
SfuI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AatII         177  GACGTC               GACGT^C
AscI          328  GGCGCGCC             GG^CGCGCC
BcgI          872  GCAACAACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          476  TGATCA               T^GATCA
BlpI          990  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      990  GCTGAGC              GC^T[ACGT]AGC
BseRI         496  GAGGAG               GAGGAG(10/8)
BssSI         172  CACGAG               CACGAG(-5/-1)
BstAPI        933  GCAGAAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        667  GGTCACC              G^GT[ACGT]ACC
BstF5I        501  GGATG                GGATG(2/0)
BstPI         667  GGTCACC              G^GT[ACGT]ACC
BstXI         509  CCAAGCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         990  GCTGAGC              GC^T[ACGT]AGC
DdeI          991  CTGAG                C^T[ACGT]AG
EarI          532  CTCTTC               CTCTTC(1/4)
EcoO65I       667  GGTCACC              G^GT[ACGT]ACC
FbaI          476  TGATCA               T^GATCA
FokI          501  GGATG                GGATG(9/13)
FseI          525  GGCCGGCC             GGCCGG^CC
HinfI           4  GAGTC                G^A[ACGT]TC
Ksp632I       532  CTCTTC               CTCTTC(1/4)
NcoI         1058  CCATGG               C^CATGG
PleI            4  GAGTC                GAGTC(4/5)
SacI         1041  GAGCTC               GAGCT^C
SgrAI         455  CGCCGGCG             C[GA]^CCGG[CT]G
SmlI          757  CTCAAG               C^T[CT][GA]AG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AfaI          438  GTAC                 GT^AC
              852  GTAC                
AspHI         169  GAGCAC               G[AT]GC[AT]^C
             1041  GAGCTC              
AviII         887  TGCGCA               TGC^GCA
              971  TGCGCA              
BpmI          427  CTGGAG               CTGGAG(16/14)
              943  CTGGAG              
BsiHKAI       169  GAGCAC               G[AT]GC[AT]^C
             1041  GAGCTC              
BsmAI         175  GAGAC                GTCTC(1/5)
              925  GAGAC               
BsmBI         175  GAGACG               CGTCTC(1/5)
              925  GAGACG              
BsrI         1002  ACTGG                ACTGG(1/-1)
              953  CCAGT               
BssHII        329  GCGCGC               G^CGCGC
              752  GCGCGC              
DpnII         477  GATC                 ^GATC
              627  GATC                
FspI          887  TGCGCA               TGC^GCA
              971  TGCGCA              
HgaI          927  GACGC                GACGC(5/10)
              446  GCGTC               
HphI          474  GGTGA                GGTGA(8/7)
              669  TCACC               
KspI           31  CCGCGG               CCGC^GG
              335  CCGCGG              
MaeII         178  ACGT                 A^CGT
              920  ACGT                
MaeIII        668  GTCAC                ^GT[ACGT]AC
              956  GTGAC               
MboI          477  GATC                 ^GATC
              627  GATC                
MboII         533  TCTTC                GAAGA(8/7)
             1022  TCTTC               
MslI          687  CACTCCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              879  CATCGCGGTG          
NdeII         477  GATC                 ^GATC
              627  GATC                
NotI          400  GCGGCCGC             GC^GGCCGC
              465  GCGGCCGC            
RsaI          438  GTAC                 GT^AC
              852  GTAC                
SacII          31  CCGCGG               CCGC^GG
              335  CCGCGG              
Sau3AI        477  GATC                 ^GATC
              627  GATC                
SfaNI         161  GCATC                GCATC(5/9)
             1019  GCATC               
SfiI          270  GGCCAAGGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              733  GGCCTGCCGGGCC       
TaqI          185  TCGA                 T^CGA
              203  TCGA                
Tsp45I        668  GTCAC                ^GT[GC]AC
              956  GTGAC               
TspRI         634  CACTG                CA[GC]TG[ACGT][ACGT]^
              954  CAGTG               
TthHB8I       185  TCGA                 T^CGA
              203  TCGA                
XcmI          272  CCAAGGCGGCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              821  CCAAGAAGTCGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        ApaI        
AvaI        BanI        BanII       BbeI        BbvI        
BcnI        BglI        BmyI        BsaHI       BsaJI       
BsiEI       BsiYI       BslI        BsoBI       Bsp1286I    
BspMI       BsrFI       BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DraII       
DsaI        EaeI        EagI        EclXI       Eco52I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HpaII       ItaI        KasI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NgoMIV      NlaIII      
NlaIV       PstI        PvuII       Sau96I      ScrFI       
SfcI        SfoI        SmaI        StyI        TseI        
XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com