Restriction map for pF1KE1842
 (Circular) (Six-base) MAPSORT of: pF1KE1842.seq  Check: 1778  from: 1  to: 4187



pF1KE1842  4187 bp

 With 182 enzymes: SgfI * 

                        January 9, 2015 15:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    170     252     731     773     170 
   Size:         82     479      42    3584

  Fragments arranged by size: 

                3584     479      82      42
 
AatII G_ACGT'C

Cuts at:    289     289 
   Size:       4187
 
AccI GT'mk_AC

Cuts at:   1225    1225 
   Size:       4187
 
Acc65I G'GTAC_C

Cuts at:   1203    1203 
   Size:       4187
 
AclI AA'CG_TT

Cuts at:   4027    4027 
   Size:       4187
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2012    2444    3191    3459    2012 
   Size:        432     747     268    2740

  Fragments arranged by size: 

                2740     747     432     268
 
AflIII A'CryG_T

Cuts at:   1539    2643    3620    1539 
   Size:       1104     977    2106

  Fragments arranged by size: 

                2106    1104     977
 
AgeI A'CCGG_T

Cuts at:   2575    2575 
   Size:       4187
 
AleI CACnn'nnGTG

Cuts at:    799     799 
   Size:       4187
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    178     178 
   Size:       4187
 
AlwNI CAG_nnn'CTG

Cuts at:   1492    3059    1492 
   Size:       1567    2620
 
ApaI G_GGCC'C

Cuts at:    223     397     451     488     853     223 
   Size:        174      54      37     365    3557

  Fragments arranged by size: 

                3557     365     174      54      37
 
ApaLI G'TGCA_C

Cuts at:   2957    2957 
   Size:       4187
 
ApoI r'AATT_y

Cuts at:   1191    3540    3796    1191 
   Size:       2349     256    1582

  Fragments arranged by size: 

                2349    1582     256
 
AscI GG'CGCG_CC

Cuts at:    437     437 
   Size:       4187
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4187
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4187
 
AvaI C'yCGr_G

Cuts at:    209     432     469    1207     209 
   Size:        223      37     738    3189

  Fragments arranged by size: 

                3189     738     223      37
 
BamHI G'GATC_C

Cuts at:   1212    1212 
   Size:       4187
 
BanI G'GyrC_C

Cuts at:    457     561     832     877    1203    1617    1865    1900     457 
   Size:        104     271      45     326     414     248      35    2744

  Fragments arranged by size: 

                2744     414     326     271     248     104      45      35
            
 
BanII G_rGCy'C

Cuts at:    172     223     397     451     488     542     599     853    1153 
   Size:         51     174      54      37      54      57     254     300

Cuts at:   1153    1201    2231     172 
   Size:         48    1030    2128

  Fragments arranged by size: 

                2128    1030     300     254     174      57      54      54
                  51      48      37
 
BbeI G_GCGC'C

Cuts at:    461     565     881    1621    1869     461 
   Size:        104     316     740     248    2779

  Fragments arranged by size: 

                2779     740     316     248     104
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    969    1003     969 
   Size:         34    4153
 
BciVI GTATCCnnnnn_n'

Cuts at:   2085    2846    4187    2085 
   Size:        761    1341    2085

  Fragments arranged by size: 

                2085    1341     761
 
BclI T'GATC_A

Cuts at:    584    1707     584 
   Size:       1123    3064
 
BglI GCCn_nnn'nGGC

Cuts at:    385     401     455     848     385 
   Size:         16      54     393    3724

  Fragments arranged by size: 

                3724     393      54      16
 
BglII A'GATC_T

Cuts at:   1702    3739    1702 
   Size:       2037    2150
 
BlpI GC'TnA_GC

Cuts at:   1099    1299    1099 
   Size:        200    3987
 
Bme1580I G_kGCm'C

Cuts at:    223     397     451     488     806     853    1812    1905    2961 
   Size:        174      54      37     318      47     959      93    1056

Cuts at:   2961     223 
   Size:       1449

  Fragments arranged by size: 

                1449    1056     959     318     174      93      54      47
                  37
 
BmrI ACTGGGnnnn_n'

Cuts at:   1399    1480    1815    3959    1399 
   Size:         81     335    2144    1627

  Fragments arranged by size: 

                2144    1627     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2554    2554 
   Size:       4187
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    556    1072     556 
   Size:        516    3671
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    850    1363    2242    2734    3032     850 
   Size:        513     879     492     298    2005

  Fragments arranged by size: 

                2005     879     513     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3891    3891 
   Size:       4187
 
BsaAI yAC'GTr

Cuts at:   1540    1540 
   Size:       4187
 
BsaBI GATnn'nnATC

Cuts at:      6    1701    3738       6 
   Size:       1695    2037     455

  Fragments arranged by size: 

                2037    1695     455
 
BsaHI Gr'CG_yC

Cuts at:    286     458     562     878    1618    1866    2568    4064     286 
   Size:        172     104     316     740     248     702    1496     409

  Fragments arranged by size: 

                1496     740     702     409     316     248     172     104
            
 
BsaWI w'CCGG_w

Cuts at:   1376    1897    2575    2849    2996    3407    1376 
   Size:        521     678     274     147     411    2156

  Fragments arranged by size: 

                2156     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    529     559     529 
   Size:         30    4157
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    619     619 
   Size:       4187
 
BseYI C'CCAG_C

Cuts at:    184     261     805     873    1612    2370    2947    3443     184 
   Size:         77     544      68     739     758     577     496     928

  Fragments arranged by size: 

                 928     758     739     577     544     496      77      68
            
 
BsiEI CG_ry'CG

Cuts at:    104     119     320     389     512     577    1729    1775    2983 
   Size:         15     201      69     123      65    1152      46    1208

Cuts at:   2983     104 
   Size:       1308

  Fragments arranged by size: 

                1308    1208    1152     201     123      69      65      46
                  15
 
BsiHKAI G_wGCw'C

Cuts at:    281    1153    1201    1979    2169    2961     281 
   Size:        872      48     778     190     792    1507

  Fragments arranged by size: 

                1507     872     792     778     190      48
 
BsmBI CGTCTCn'nnnn_

Cuts at:    277    1027     277 
   Size:        750    3437
 
Bsp1286I G_dGCh'C

Cuts at:    172     223     281     397     451     488     542     599     806 
   Size:         51      58     116      54      37      54      57     207

Cuts at:    806     853    1153    1201    1812    1905    1979    2169    2231 
   Size:         47     300      48     611      93      74     190      62

Cuts at:   2231    2961     172 
   Size:        730    1398

  Fragments arranged by size: 

                1398     730     611     300     207     190     116      93
                  74      62      58      57      54      54      51      48
                  47      37
 
BspEI T'CCGG_A

Cuts at:   3407    3407 
   Size:       4187
 
BspHI T'CATG_A

Cuts at:   3363    3363 
   Size:       4187
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    170     252     731     773    1237    1451    1753    2134     170 
   Size:         82     479      42     464     214     302     381    2223

  Fragments arranged by size: 

                2223     479     464     381     302     214      82      42
            
 
BsrBI CCG'CTC

Cuts at:     50    2479    2533    4017    4182      50 
   Size:       2429      54    1484     165      55

  Fragments arranged by size: 

                2429    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2099    2099 
   Size:       4187
 
BsrFI r'CCGG_y

Cuts at:    362     514     564     634     838    2185    2366    2575     362 
   Size:        152      50      70     204    1347     181     209    1974

  Fragments arranged by size: 

                1974    1347     209     204     181     152      70      50
            
 
BssHII G'CGCG_C

Cuts at:    437     860     437 
   Size:        423    3764
 
BssSI C'ACGA_G

Cuts at:    280    2458    2816     280 
   Size:       2178     358    1651

  Fragments arranged by size: 

                2178    1651     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1047    1564    1047 
   Size:        517    3670
 
BstBI TT'CG_AA

Cuts at:   2548    2548 
   Size:       4187
 
BstEII G'GTnAC_C

Cuts at:    775     775 
   Size:       4187
 
BstXI CCAn_nnnn'nTGG

Cuts at:    624     624 
   Size:       4187
 
BstYI r'GATC_y

Cuts at:      1    1212    1679    1702    2037    2283    3295    3381    3393 
   Size:       1211     467      23     335     246    1012      86      12

Cuts at:   3393    3739       1 
   Size:        346     449

  Fragments arranged by size: 

                1211    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     139     389     398     443    1166     107 
   Size:         32     250       9      45     723    3128

  Fragments arranged by size: 

                3128     723     250      45      32       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    972    2137    2318    2481     972 
   Size:       1165     181     163    2678

  Fragments arranged by size: 

                2678    1165     181     163
 
BtsI GCAGTG_nn'

Cuts at:    741    1430     741 
   Size:        689    3498
 
DraI TTT'AAA

Cuts at:   1186    1186 
   Size:       4187
 
DrdI GACnn_nn'nnGTC

Cuts at:   1893    2751    1893 
   Size:        858    3329
 
EaeI y'GGCC_r

Cuts at:    116     317     341     386     509     574     632     710     747 
   Size:        201      24      45     123      65      58      78      37

Cuts at:    747    1772    1946    2337    2364    3709     116 
   Size:       1025     174     391      27    1345     594

  Fragments arranged by size: 

                1345    1025     594     391     201     174     123      78
                  65      58      45      37      27      24
 
EagI C'GGCC_G

Cuts at:    116     317     386     509     574    1772     116 
   Size:        201      69     123      65    1198    2531

  Fragments arranged by size: 

                2531    1198     201     123      69      65
 
EarI CTCTTCn'nnn_

Cuts at:    646    2210    2420     646 
   Size:       1564     210    2413

  Fragments arranged by size: 

                2413    1564     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    148    1123    2395    2705    2851    3997     148 
   Size:        975    1272     310     146    1146     338

  Fragments arranged by size: 

                1272    1146     975     338     310     146
 
EcoICRI GAG'CTC

Cuts at:   1151    1199    1151 
   Size:         48    4139
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    556    1072    2012    2444    3191    3459     556 
   Size:        516     940     432     747     268    1284

  Fragments arranged by size: 

                1284     940     747     516     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    220     394     448     485     850    1326     220 
   Size:        174      54      37     365     476    3081

  Fragments arranged by size: 

                3081     476     365     174      54      37
 
EcoRI G'AATT_C

Cuts at:   1191    1191 
   Size:       4187
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2206    2206 
   Size:       4187
 
FseI GG_CCGG'CC

Cuts at:    638     638 
   Size:       4187
 
FspI TGC'GCA

Cuts at:    997    1081    1968     997 
   Size:         84     887    3216

  Fragments arranged by size: 

                3216     887      84
 
HaeII r_GCGC'y

Cuts at:    194     305     461     565     571     631     881    1621    1869 
   Size:        111     156     104       6      60     250     740     248

Cuts at:   1869    2891    3870     194 
   Size:       1022     979     511

  Fragments arranged by size: 

                1022     979     740     511     250     248     156     111
                 104      60       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2029    2059    2061    2091    2029 
   Size:         30       2      30    4125

  Fragments arranged by size: 

                4125      30      30       2
 
HincII GTy'rAC

Cuts at:   1226    1226 
   Size:       4187
 
KasI G'GCGC_C

Cuts at:    457     561     877    1617    1865     457 
   Size:        104     316     740     248    2779

  Fragments arranged by size: 

                2779     740     316     248     104
 
KpnI G_GTAC'C

Cuts at:   1207    1207 
   Size:       4187
 
MluI A'CGCG_T

Cuts at:   3620    3620 
   Size:       4187
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2858    3042    2858 
   Size:        184    4003
 
MscI TGG'CCA

Cuts at:   1948    1948 
   Size:       4187
 
MslI CAynn'nnrTG

Cuts at:    799     991    2176    2303     799 
   Size:        192    1185     127    2683

  Fragments arranged by size: 

                2683    1185     192     127
 
MspA1I CmG'CkG

Cuts at:    141     261     445     579     624     914    1098    1140    1298 
   Size:        120     184     134      45     290     184      42     158

Cuts at:   1298    1612    1972    2985    3230     141 
   Size:        314     360    1013     245    1098

  Fragments arranged by size: 

                1098    1013     360     314     290     245     184     184
                 158     134     120      45      42
 
NaeI GCC'GGC

Cuts at:    364     516     566     636     840    2368     364 
   Size:        152      50      70     204    1528    2183

  Fragments arranged by size: 

                2183    1528     204     152      70      50
 
NarI GG'CG_CC

Cuts at:    458     562     878    1618    1866     458 
   Size:        104     316     740     248    2779

  Fragments arranged by size: 

                2779     740     316     248     104
 
NcoI C'CATG_G

Cuts at:    107    1166     107 
   Size:       1059    3128
 
NgoMIV G'CCGG_C

Cuts at:    362     514     564     634     838    2366     362 
   Size:        152      50      70     204    1528    2183

  Fragments arranged by size: 

                2183    1528     204     152      70      50
 
NotI GC'GGCC_GC

Cuts at:    509     574     509 
   Size:         65    4122
 
NspI r_CATG'y

Cuts at:   1240    1740    2647    1240 
   Size:        500     907    2780

  Fragments arranged by size: 

                2780     907     500
 
PciI A'CATG_T

Cuts at:   2643    2643 
   Size:       4187
 
PmeI GTTT'AAAC

Cuts at:   1186    1186 
   Size:       4187
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3326    3358    3326 
   Size:         32    4155
 
PspOMI G'GGCC_C

Cuts at:    219     393     447     484     849     219 
   Size:        174      54      37     365    3557

  Fragments arranged by size: 

                3557     365     174      54      37
 
PstI C_TGCA'G

Cuts at:    167     728     770    1234    1919     167 
   Size:        561      42     464     685    2435

  Fragments arranged by size: 

                2435     685     561     464      42
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4187
 
PvuII CAG'CTG

Cuts at:    624    1098    1140    1612    1972     624 
   Size:        474      42     472     360    2839

  Fragments arranged by size: 

                2839     474     472     360      42
 
RsrII CG'GwC_CG

Cuts at:   2382    2382 
   Size:       4187
 
SacI G_AGCT'C

Cuts at:   1153    1201    1153 
   Size:         48    4139
 
SacII CC_GC'GG

Cuts at:    142     446     142 
   Size:        304    3883
 
SalI G'TCGA_C

Cuts at:   1224    1224 
   Size:       4187
 
SapI GCTCTTCn'nnn_

Cuts at:   2210    2420    2210 
   Size:        210    3977
 
SbfI CC_TGCA'GG

Cuts at:   1234    1234 
   Size:       4187
 
ScaI AGT'ACT

Cuts at:   3704    3704 
   Size:       4187
 
SfcI C'TryA_G

Cuts at:     33     163     724     766    1230    1915    2908    3099      33 
   Size:        130     561      42     464     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     561     464     191     130      42
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    385     848     385 
   Size:        463    3724
 
SfoI GGC'GCC

Cuts at:    459     563     879    1619    1867     459 
   Size:        104     316     740     248    2779

  Fragments arranged by size: 

                2779     740     316     248     104
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4187
 
SgrAI Cr'CCGG_yG

Cuts at:    564     564 
   Size:       4187
 
SmaI CCC'GGG

Cuts at:    211     434     471    1209     211 
   Size:        223      37     738    3189

  Fragments arranged by size: 

                3189     738     223      37
 
SmlI C'TyrA_G

Cuts at:    865    1342    2257    2749    3011     865 
   Size:        477     915     492     262    2041

  Fragments arranged by size: 

                2041     915     492     477     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4187
 
SphI G_CATG'C

Cuts at:   1240    1740    1240 
   Size:        500    3687
 
StyI C'CwwG_G

Cuts at:    107     380    1154    1166    1321    1675     107 
   Size:        273     774      12     155     354    2619

  Fragments arranged by size: 

                2619     774     354     273     155      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1770    1903    2362    2571    1770 
   Size:        133     459     209    3386

  Fragments arranged by size: 

                3386     459     209     133
 
TatI w'GTAC_w

Cuts at:   3702    3702 
   Size:       4187
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    271     303    3704    3736     271 
   Size:         32    3401      32     722

  Fragments arranged by size: 

                3401     722      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1984    1984 
   Size:       4187
 
XbaI T'CTAG_A

Cuts at:   1218    1218 
   Size:       4187
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    387     936    1682     387 
   Size:        549     746    2892

  Fragments arranged by size: 

                2892     746     549
 
XmaI C'CCGG_G

Cuts at:    209     432     469    1207     209 
   Size:        223      37     738    3189

  Fragments arranged by size: 

                3189     738     223      37
 
ZraI GAC'GTC

Cuts at:    287     287 
   Size:       4187
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FseI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NotI     NspI     PciI     PmeI     PpiI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AhdI     AloI    AvrII     BaeI     BbsI    BbvCI
    BfrBI    BmgBI     BmtI   Bpu10I     BsgI    BsiWI     BsmI    BsrGI
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI    PspXI
     PsrI    SanDI    SexAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     XmnI
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