Restriction map for
pF1KE0151
(Circular) (Six-base) MAPSORT of: pF1KE0151.seq Check: 272 from: 1 to: 3470
pF1KE0151 3470 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 508 508
Size: 3470
Acc65I G'GTAC_C
Cuts at: 486 486
Size: 3470
AclI AA'CG_TT
Cuts at: 3310 3310
Size: 3470
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 1295 1727 2474 2742 431
Size: 864 432 747 268 1159
Fragments arranged by size:
1159 864 747 432 268
AflIII A'CryG_T
Cuts at: 462 822 1926 2903 462
Size: 360 1104 977 1029
Fragments arranged by size:
1104 1029 977 360
AgeI A'CCGG_T
Cuts at: 1858 1858
Size: 3470
AlwNI CAG_nnn'CTG
Cuts at: 775 2342 775
Size: 1567 1903
ApaLI G'TGCA_C
Cuts at: 2240 2240
Size: 3470
ApoI r'AATT_y
Cuts at: 437 448 474 2823 3079 437
Size: 11 26 2349 256 828
Fragments arranged by size:
2349 828 256 26 11
AseI AT'TA_AT
Cuts at: 20 20
Size: 3470
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3470
AvaI C'yCGr_G
Cuts at: 490 490
Size: 3470
BamHI G'GATC_C
Cuts at: 495 495
Size: 3470
BanI G'GyrC_C
Cuts at: 486 900 1148 1183 486
Size: 414 248 35 2773
Fragments arranged by size:
2773 414 248 35
BanII G_rGCy'C
Cuts at: 484 1514 484
Size: 1030 2440
BbeI G_GCGC'C
Cuts at: 904 1152 904
Size: 248 3222
BbsI GAAGACnn'nnnn_
Cuts at: 347 347
Size: 3470
BciVI GTATCCnnnnn_n'
Cuts at: 1368 2129 3470 1368
Size: 761 1341 1368
Fragments arranged by size:
1368 1341 761
BclI T'GATC_A
Cuts at: 990 990
Size: 3470
BglII A'GATC_T
Cuts at: 985 3022 985
Size: 2037 1433
BlpI GC'TnA_GC
Cuts at: 582 582
Size: 3470
Bme1580I G_kGCm'C
Cuts at: 1095 1188 2244 1095
Size: 93 1056 2321
Fragments arranged by size:
2321 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 682 763 1098 3242 682
Size: 81 335 2144 910
Fragments arranged by size:
2144 910 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1837 1837
Size: 3470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1525 2017 2315 646
Size: 879 492 298 1801
Fragments arranged by size:
1801 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3174 3174
Size: 3470
BsaAI yAC'GTr
Cuts at: 463 823 463
Size: 360 3110
BsaBI GATnn'nnATC
Cuts at: 6 984 3021 6
Size: 978 2037 455
Fragments arranged by size:
2037 978 455
BsaHI Gr'CG_yC
Cuts at: 901 1149 1851 3347 901
Size: 248 702 1496 1024
Fragments arranged by size:
1496 1024 702 248
BsaWI w'CCGG_w
Cuts at: 659 1180 1858 2132 2279 2690 659
Size: 521 678 274 147 411 1439
Fragments arranged by size:
1439 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 187
Size: 3470
BseYI C'CCAG_C
Cuts at: 895 1653 2230 2726 895
Size: 758 577 496 1639
Fragments arranged by size:
1639 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 263 1012 1058 2266 104
Size: 159 749 46 1208 1308
Fragments arranged by size:
1308 1208 749 159 46
BsiHKAI G_wGCw'C
Cuts at: 484 1262 1452 2244 484
Size: 778 190 792 1710
Fragments arranged by size:
1710 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 484 1095 1188 1262 1452 1514 2244 484
Size: 611 93 74 190 62 730 1710
Fragments arranged by size:
1710 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2690 2690
Size: 3470
BspHI T'CATG_A
Cuts at: 2646 2646
Size: 3470
BspMI ACCTGCnnnn'nnnn_
Cuts at: 520 734 1036 1417 520
Size: 214 302 381 2573
Fragments arranged by size:
2573 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1762 1816 3300 3465 50
Size: 1712 54 1484 165 55
Fragments arranged by size:
1712 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1382 1382
Size: 3470
BsrFI r'CCGG_y
Cuts at: 1468 1649 1858 1468
Size: 181 209 3080
Fragments arranged by size:
3080 209 181
BssSI C'ACGA_G
Cuts at: 1741 2099 1741
Size: 358 3112
BstAPI GCAn_nnn'nTGC
Cuts at: 847 847
Size: 3470
BstBI TT'CG_AA
Cuts at: 1831 1831
Size: 3470
BstYI r'GATC_y
Cuts at: 1 495 962 985 1320 1566 2578 2664 2676
Size: 494 467 23 335 246 1012 86 12
Cuts at: 2676 3022 1
Size: 346 449
Fragments arranged by size:
1012 494 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 190 190
Size: 3470
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 265 1420 1601 1764 265
Size: 1155 181 163 1971
Fragments arranged by size:
1971 1155 181 163
BtsI GCAGTG_nn'
Cuts at: 713 713
Size: 3470
DraI TTT'AAA
Cuts at: 469 469
Size: 3470
DrdI GACnn_nn'nnGTC
Cuts at: 1176 2034 1176
Size: 858 2612
EaeI y'GGCC_r
Cuts at: 1055 1229 1620 1647 2992 1055
Size: 174 391 27 1345 1533
Fragments arranged by size:
1533 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1055 1055
Size: 3470
EarI CTCTTCn'nnn_
Cuts at: 1493 1703 1493
Size: 210 3260
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 135 267 1678 1988 2134 3280 135
Size: 132 1411 310 146 1146 325
Fragments arranged by size:
1411 1146 325 310 146 132
EcoICRI GAG'CTC
Cuts at: 482 482
Size: 3470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 1295 1727 2474 2742 431
Size: 864 432 747 268 1159
Fragments arranged by size:
1159 864 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 609 609
Size: 3470
EcoRI G'AATT_C
Cuts at: 474 474
Size: 3470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1489 1489
Size: 3470
FspI TGC'GCA
Cuts at: 1251 1251
Size: 3470
HaeII r_GCGC'y
Cuts at: 904 1152 2174 3153 904
Size: 248 1022 979 1221
Fragments arranged by size:
1221 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1312 1342 1344 1374 1312
Size: 30 2 30 3408
Fragments arranged by size:
3408 30 30 2
HincII GTy'rAC
Cuts at: 509 509
Size: 3470
KasI G'GCGC_C
Cuts at: 900 1148 900
Size: 248 3222
KpnI G_GTAC'C
Cuts at: 490 490
Size: 3470
MluI A'CGCG_T
Cuts at: 2903 2903
Size: 3470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2141 2325 2141
Size: 184 3286
MscI TGG'CCA
Cuts at: 1231 1231
Size: 3470
MslI CAynn'nnrTG
Cuts at: 1459 1586 1459
Size: 127 3343
MspA1I CmG'CkG
Cuts at: 192 581 895 1255 2268 2513 192
Size: 389 314 360 1013 245 1149
Fragments arranged by size:
1149 1013 389 360 314 245
NaeI GCC'GGC
Cuts at: 1651 1651
Size: 3470
NarI GG'CG_CC
Cuts at: 901 1149 901
Size: 248 3222
NdeI CA'TA_TG
Cuts at: 429 429
Size: 3470
NgoMIV G'CCGG_C
Cuts at: 1649 1649
Size: 3470
NruI TCG'CGA
Cuts at: 265 265
Size: 3470
NspI r_CATG'y
Cuts at: 523 1023 1930 523
Size: 500 907 2063
Fragments arranged by size:
2063 907 500
PciI A'CATG_T
Cuts at: 1926 1926
Size: 3470
PmeI GTTT'AAAC
Cuts at: 469 469
Size: 3470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2609 2641 2609
Size: 32 3438
PstI C_TGCA'G
Cuts at: 517 1202 517
Size: 685 2785
PvuI CG_AT'CG
Cuts at: 104 263 104
Size: 159 3311
PvuII CAG'CTG
Cuts at: 895 1255 895
Size: 360 3110
RsrII CG'GwC_CG
Cuts at: 1665 1665
Size: 3470
SacI G_AGCT'C
Cuts at: 484 484
Size: 3470
SacII CC_GC'GG
Cuts at: 193 193
Size: 3470
SalI G'TCGA_C
Cuts at: 507 507
Size: 3470
SapI GCTCTTCn'nnn_
Cuts at: 1493 1703 1493
Size: 210 3260
SbfI CC_TGCA'GG
Cuts at: 517 517
Size: 3470
ScaI AGT'ACT
Cuts at: 2987 2987
Size: 3470
SfcI C'TryA_G
Cuts at: 33 513 1198 2191 2382 33
Size: 480 685 993 191 1121
Fragments arranged by size:
1121 993 685 480 191
SfoI GGC'GCC
Cuts at: 902 1150 902
Size: 248 3222
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3470
SmaI CCC'GGG
Cuts at: 492 492
Size: 3470
SmlI C'TyrA_G
Cuts at: 625 1540 2032 2294 625
Size: 915 492 262 1801
Fragments arranged by size:
1801 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3470
SphI G_CATG'C
Cuts at: 523 1023 523
Size: 500 2970
StyI C'CwwG_G
Cuts at: 604 958 604
Size: 354 3116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 199 1053 1186 1645 1854 199
Size: 854 133 459 209 1815
Fragments arranged by size:
1815 854 459 209 133
TatI w'GTAC_w
Cuts at: 2985 2985
Size: 3470
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3438
Tth111I GACn'n_nGTC
Cuts at: 1267 1267
Size: 3470
XbaI T'CTAG_A
Cuts at: 501 501
Size: 3470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 965 965
Size: 3470
XmaI C'CCGG_G
Cuts at: 490 490
Size: 3470
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NruI NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI