Restriction map for pF1KA0533
##################################
# NO CUT ENZYMES (   38 enzymes) #
##################################

AclI        AflII       AseI        AsnI        Asp700I     
AvrII       BfrI        BlnI        BsiWI       Bst1107I    
BstBI       BstZ17I     CpoI        DraI        EcoRI       
EcoT22I     HindIII     HpaI        MluI        MseI        
NdeI        NruI        NsiI        NspV        PacI        
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SfuI        SnaBI       SpeI        SspI        SwaI        
Tru9I       XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AcsI          726  GAATTT               [GA]^AATT[CT]
AhdI         5107  GACCGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          726  GAATTT               [GA]^AATT[CT]
AscI         9365  GGCGCGCC             GG^CGCGCC
AspEI        5107  GACCGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI        7012  GCTGAGG              CCTCAGC(-5/-2)
BclI         3260  TGATCA               T^GATCA
BsaBI        5223  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        6682  GAGACG               CGTCTC(1/5)
BsmI         1987  GAATGC               GAATGC(1/-1)
Eam1105I     5107  GACCGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV         877  GATATC               GAT^ATC
FbaI         3260  TGATCA               T^GATCA
KspI          402  CCGCGG               CCGC^GG
MamI         5223  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         6787  CAATTG               C^AATTG
MunI         6787  CAATTG               C^AATTG
NheI        10806  GCTAGC               G^CTAGC
NotI         5795  GCGGCCGC             GC^GGCCGC
PaeR7I       2686  CTCGAG               C^TCGAG
PshAI        2532  GACGGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         402  CCGCGG               CCGC^GG
SalI         8799  GTCGAC               G^TCGAC
SexAI        5820  ACCTGGT              A^CC[AT]GGT
SgrAI        1200  CACCGGCG             C[GA]^CCGG[CT]G
TfiI         5861  GATTC                G^A[AT]TC
XhoI         2686  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Acc65I       8326  GGTACC               G^GTACC
             8639  GGTACC              
AccIII       6656  TCCGGA               T^CCGGA
             6938  TCCGGA              
AgeI         1850  ACCGGT               A^CCGGT
            10997  ACCGGT              
Asp718I      8326  GGTACC               G^GTACC
             8639  GGTACC              
AspI         1538  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             8617  GACTTCGTC           
BamHI        2199  GGATCC               G^GATCC
            10307  GGATCC              
BciVI        3468  GGATAC               GTATCC(6/5)
             4420  GGATAC              
BglII        5402  AGATCT               A^GATCT
             6011  AGATCT              
BseAI        6656  TCCGGA               T^CCGGA
             6938  TCCGGA              
BspDI         361  ATCGAT               AT^CGAT
             8458  ATCGAT              
BspEI        6656  TCCGGA               T^CCGGA
             6938  TCCGGA              
BspHI        5348  TCATGA               T^CATGA
             5696  TCATGA              
ClaI          361  ATCGAT               AT^CGAT
             8458  ATCGAT              
FseI         1803  GGCCGGCC             GGCCGG^CC
             7134  GGCCGGCC            
KpnI         8326  GGTACC               GGTAC^C
             8639  GGTACC              
MroI         6656  TCCGGA               T^CCGGA
             6938  TCCGGA              
PflFI        1538  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             8617  GACTTCGTC           
PinAI        1850  ACCGGT               A^CCGGT
            10997  ACCGGT              
RcaI         5348  TCATGA               T^CATGA
             5696  TCATGA              
ScaI          302  AGTACT               AGT^ACT
              653  AGTACT              
Tth111I      1538  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             8617  GACTTCGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  172 enzymes) #
#########################################

AatII       AccI        AccII       AciI        AcyI        
AfaI        AflIII      AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AspHI       AvaI        AvaII       AviII       BalI        
BanI        BanII       BbeI        BbrPI       BbsI        
BbvI        BcgI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DrdI        
DsaI        EaeI        EagI        EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      SbfI        ScrFI       
SfaNI       SfcI        SfiI        SfoI        SmaI        
SmlI        SphI        Sse8387I    SspBI       StuI        
StyI        TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        
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