Restriction map for pF1KE0128
 (Circular) (Six-base) MAPSORT of: pF1KE0128.seq  Check: 7949  from: 1  to: 3416



pF1KE0128  3416 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AccI GT'mk_AC

Cuts at:    454     454 
   Size:       3416
 
Acc65I G'GTAC_C

Cuts at:    432     432 
   Size:       3416
 
AclI AA'CG_TT

Cuts at:   3256    3256 
   Size:       3416
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    208    1241    1673    2420    2688     208 
   Size:       1033     432     747     268     936

  Fragments arranged by size: 

                1033     936     747     432     268
 
AflIII A'CryG_T

Cuts at:    768    1872    2849     768 
   Size:       1104     977    1335

  Fragments arranged by size: 

                1335    1104     977
 
AgeI A'CCGG_T

Cuts at:   1804    1804 
   Size:       3416
 
AleI CACnn'nnGTG

Cuts at:    387     387 
   Size:       3416
 
AlwNI CAG_nnn'CTG

Cuts at:    721    2288     721 
   Size:       1567    1849
 
ApaLI G'TGCA_C

Cuts at:   2186    2186 
   Size:       3416
 
ApoI r'AATT_y

Cuts at:    420    2769    3025     420 
   Size:       2349     256     811

  Fragments arranged by size: 

                2349     811     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3416
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3416
 
AvaI C'yCGr_G

Cuts at:    436     436 
   Size:       3416
 
BamHI G'GATC_C

Cuts at:    441     441 
   Size:       3416
 
BanI G'GyrC_C

Cuts at:    271     432     846    1094    1129     271 
   Size:        161     414     248      35    2558

  Fragments arranged by size: 

                2558     414     248     161      35
 
BanII G_rGCy'C

Cuts at:    430    1460     430 
   Size:       1030    2386
 
BbeI G_GCGC'C

Cuts at:    275     850    1098     275 
   Size:        575     248    2593

  Fragments arranged by size: 

                2593     575     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    196     196 
   Size:       3416
 
BciVI GTATCCnnnnn_n'

Cuts at:   1314    2075    3416    1314 
   Size:        761    1341    1314

  Fragments arranged by size: 

                1341    1314     761
 
BclI T'GATC_A

Cuts at:    218     936     218 
   Size:        718    2698
 
BglII A'GATC_T

Cuts at:    931    2968     931 
   Size:       2037    1379
 
BlpI GC'TnA_GC

Cuts at:    528     528 
   Size:       3416
 
Bme1580I G_kGCm'C

Cuts at:   1041    1134    2190    1041 
   Size:         93    1056    2267

  Fragments arranged by size: 

                2267    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    628     709    1044    3188     628 
   Size:         81     335    2144     856

  Fragments arranged by size: 

                2144     856     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1783    1783 
   Size:       3416
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    592    1471    1963    2261     592 
   Size:        879     492     298    1747

  Fragments arranged by size: 

                1747     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3120    3120 
   Size:       3416
 
BsaAI yAC'GTr

Cuts at:    769     769 
   Size:       3416
 
BsaBI GATnn'nnATC

Cuts at:      6     930    2967       6 
   Size:        924    2037     455

  Fragments arranged by size: 

                2037     924     455
 
BsaHI Gr'CG_yC

Cuts at:    272     847    1095    1797    3293     272 
   Size:        575     248     702    1496     395

  Fragments arranged by size: 

                1496     702     575     395     248
 
BsaWI w'CCGG_w

Cuts at:    605    1126    1804    2078    2225    2636     605 
   Size:        521     678     274     147     411    1385

  Fragments arranged by size: 

                1385     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    841    1599    2176    2672     841 
   Size:        758     577     496    1585

  Fragments arranged by size: 

                1585     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     958    1004    2212     104 
   Size:        854      46    1208    1308

  Fragments arranged by size: 

                1308    1208     854      46
 
BsiHKAI G_wGCw'C

Cuts at:    430    1208    1398    2190     430 
   Size:        778     190     792    1656

  Fragments arranged by size: 

                1656     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    430    1041    1134    1208    1398    1460    2190     430 
   Size:        611      93      74     190      62     730    1656

  Fragments arranged by size: 

                1656     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2636    2636 
   Size:       3416
 
BspHI T'CATG_A

Cuts at:   2592    2592 
   Size:       3416
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    466     680     982    1363     466 
   Size:        214     302     381    2519

  Fragments arranged by size: 

                2519     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1708    1762    3246    3411      50 
   Size:       1658      54    1484     165      55

  Fragments arranged by size: 

                1658    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1328    1328 
   Size:       3416
 
BsrFI r'CCGG_y

Cuts at:   1414    1595    1804    1414 
   Size:        181     209    3026

  Fragments arranged by size: 

                3026     209     181
 
BssSI C'ACGA_G

Cuts at:   1687    2045    1687 
   Size:        358    3058
 
BstAPI GCAn_nnn'nTGC

Cuts at:    793     793 
   Size:       3416
 
BstBI TT'CG_AA

Cuts at:   1777    1777 
   Size:       3416
 
BstYI r'GATC_y

Cuts at:      1     441     908     931    1266    1512    2524    2610    2622 
   Size:        440     467      23     335     246    1012      86      12

Cuts at:   2622    2968       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     449     440     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3416
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1366    1547    1710    1366 
   Size:        181     163    3072

  Fragments arranged by size: 

                3072     181     163
 
BtsI GCAGTG_nn'

Cuts at:    139     329     659     139 
   Size:        190     330    2896

  Fragments arranged by size: 

                2896     330     190
 
DraI TTT'AAA

Cuts at:    415     415 
   Size:       3416
 
DrdI GACnn_nn'nnGTC

Cuts at:   1122    1980    1122 
   Size:        858    2558
 
EaeI y'GGCC_r

Cuts at:   1001    1175    1566    1593    2938    1001 
   Size:        174     391      27    1345    1479

  Fragments arranged by size: 

                1479    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1001    1001 
   Size:       3416
 
EarI CTCTTCn'nnn_

Cuts at:   1439    1649    1439 
   Size:        210    3206
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1624    1934    2080    3226    1624 
   Size:        310     146    1146    1814

  Fragments arranged by size: 

                1814    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    428     428 
   Size:       3416
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    208    1241    1673    2420    2688     208 
   Size:       1033     432     747     268     936

  Fragments arranged by size: 

                1033     936     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    555     555 
   Size:       3416
 
EcoRI G'AATT_C

Cuts at:    420     420 
   Size:       3416
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    292    1435     292 
   Size:       1143    2273
 
FspI TGC'GCA

Cuts at:   1197    1197 
   Size:       3416
 
HaeII r_GCGC'y

Cuts at:    117     133     275     850    1098    2120    3099     117 
   Size:         16     142     575     248    1022     979     434

  Fragments arranged by size: 

                1022     979     575     434     248     142      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1258    1288    1290    1320    1258 
   Size:         30       2      30    3354

  Fragments arranged by size: 

                3354      30      30       2
 
HincII GTy'rAC

Cuts at:    455     455 
   Size:       3416
 
KasI G'GCGC_C

Cuts at:    271     846    1094     271 
   Size:        575     248    2593

  Fragments arranged by size: 

                2593     575     248
 
KpnI G_GTAC'C

Cuts at:    436     436 
   Size:       3416
 
MluI A'CGCG_T

Cuts at:   2849    2849 
   Size:       3416
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2087    2271    2087 
   Size:        184    3232
 
MscI TGG'CCA

Cuts at:   1177    1177 
   Size:       3416
 
MslI CAynn'nnrTG

Cuts at:    387    1405    1532     387 
   Size:       1018     127    2271

  Fragments arranged by size: 

                2271    1018     127
 
MspA1I CmG'CkG

Cuts at:    527     841    1201    2214    2459     527 
   Size:        314     360    1013     245    1484

  Fragments arranged by size: 

                1484    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1597    1597 
   Size:       3416
 
NarI GG'CG_CC

Cuts at:    272     847    1095     272 
   Size:        575     248    2593

  Fragments arranged by size: 

                2593     575     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3416
 
NgoMIV G'CCGG_C

Cuts at:   1595    1595 
   Size:       3416
 
NspI r_CATG'y

Cuts at:    469     969    1876     469 
   Size:        500     907    2009

  Fragments arranged by size: 

                2009     907     500
 
PciI A'CATG_T

Cuts at:   1872    1872 
   Size:       3416
 
PmeI GTTT'AAAC

Cuts at:    415     415 
   Size:       3416
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2555    2587    2555 
   Size:         32    3384
 
PstI C_TGCA'G

Cuts at:    146     463    1148     146 
   Size:        317     685    2414

  Fragments arranged by size: 

                2414     685     317
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3416
 
PvuII CAG'CTG

Cuts at:    841    1201     841 
   Size:        360    3056
 
RsrII CG'GwC_CG

Cuts at:   1611    1611 
   Size:       3416
 
SacI G_AGCT'C

Cuts at:    430     430 
   Size:       3416
 
SalI G'TCGA_C

Cuts at:    453     453 
   Size:       3416
 
SapI GCTCTTCn'nnn_

Cuts at:   1439    1649    1439 
   Size:        210    3206
 
SbfI CC_TGCA'GG

Cuts at:    463     463 
   Size:       3416
 
ScaI AGT'ACT

Cuts at:   2933    2933 
   Size:       3416
 
SfcI C'TryA_G

Cuts at:     33     142     459    1144    2137    2328      33 
   Size:        109     317     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     317     191     109
 
SfoI GGC'GCC

Cuts at:    273     848    1096     273 
   Size:        575     248    2593

  Fragments arranged by size: 

                2593     575     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3416
 
SmaI CCC'GGG

Cuts at:    438     438 
   Size:       3416
 
SmlI C'TyrA_G

Cuts at:    571    1486    1978    2240     571 
   Size:        915     492     262    1747

  Fragments arranged by size: 

                1747     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3416
 
SphI G_CATG'C

Cuts at:    469     969     469 
   Size:        500    2916
 
StyI C'CwwG_G

Cuts at:    107     276     550     904     107 
   Size:        169     274     354    2619

  Fragments arranged by size: 

                2619     354     274     169
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    999    1132    1591    1800     999 
   Size:        133     459     209    2615

  Fragments arranged by size: 

                2615     459     209     133
 
TatI w'GTAC_w

Cuts at:   2931    2931 
   Size:       3416
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2933    2965    2933 
   Size:         32    3384
 
Tth111I GACn'n_nGTC

Cuts at:   1213    1213 
   Size:       3416
 
XbaI T'CTAG_A

Cuts at:    447     447 
   Size:       3416
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    911     911 
   Size:       3416
 
XmaI C'CCGG_G

Cuts at:    436     436 
   Size:       3416
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI     PmeI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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