Restriction map for pF1KB3755
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AccI        AccIII      AclI        AcsI        AflIII      
AgeI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BclI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmI        Bsp1407I    BspEI       BspLU11I    BsrGI       
Bst1107I    BstAPI      BstBI       BstXI       BstZ17I     
CelII       CpoI        DraI        DraII       DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoO109I    EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
HpaI        KasI        KspI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SpeI        SphI        SspBI       SspI        SwaI        
Tth111I     XbaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII        1324  GACGTC               GACGT^C
Acc65I        160  GGTACC               G^GTACC
AccII        1750  CGCG                 CG^CG
AcyI         1324  GACGTC               G[GA]^CG[CT]C
AflII         321  CTTAAG               C^TTAAG
AlwI          758  GGATC                GGATC(4/5)
Asp718I       160  GGTACC               G^GTACC
BalI         1388  TGGCCA               TGG^CCA
BcgI          701  GCACAGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI          59  GGATAC               GTATCC(6/5)
BfaI         1291  CTAG                 C^TAG
BfrI          321  CTTAAG               C^TTAAG
BglI         1381  GCCACTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI        1324  GACGTC               G[GA]^CG[CT]C
BsmBI        1078  GAGACG               CGTCTC(1/5)
BspDI         395  ATCGAT               AT^CGAT
BspHI         389  TCATGA               T^CATGA
BsrBI         517  CCGCTC               CCGCTC(-3/-3)
BsrDI         455  GCAATG               GCAATG(2/0)
BsrFI         553  GCCGGT               [GA]^CCGG[CT]
BssHII       1749  GCGCGC               G^CGCGC
BssSI         510  CACGAG               CACGAG(-5/-1)
BstEII        240  GGTGACC              G^GT[ACGT]ACC
BstPI         240  GGTGACC              G^GT[ACGT]ACC
BstUI        1750  CGCG                 CG^CG
BstYI         758  GGATCT               [GA]^GATC[CT]
Cfr10I        553  GCCGGT               [GA]^CCGG[CT]
ClaI          395  ATCGAT               AT^CGAT
EarI         1233  GAAGAG               CTCTTC(1/4)
EcoO65I       240  GGTGACC              G^GT[ACGT]ACC
EcoT14I       236  CCTTGG               C^C[AT][AT]GG
Hin1I        1324  GACGTC               G[GA]^CG[CT]C
KpnI          160  GGTACC               GGTAC^C
Ksp632I      1233  GAAGAG               CTCTTC(1/4)
MaeI         1291  CTAG                 C^TAG
MflI          758  GGATCT               [GA]^GATC[CT]
MluNI        1388  TGGCCA               TGG^CCA
MscI         1388  TGGCCA               TGG^CCA
MvnI         1750  CGCG                 CG^CG
PaeR7I        128  CTCGAG               C^TCGAG
PflMI         950  CCAAGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1294  GAGTC                GAGTC(4/5)
RcaI          389  TCATGA               T^CATGA
SapI         1233  GAAGAGC              GCTCTTC(1/4)
SbfI         1164  CCTGCAGG             CCTGCA^GG
SmaI           90  CCCGGG               CCC^GGG
SnaBI         445  TACGTA               TAC^GTA
Sse8387I     1164  CCTGCAGG             CCTGCA^GG
StuI         1763  AGGCCT               AGG^CCT
StyI          236  CCTTGG               C^C[AT][AT]GG
Van91I        950  CCAAGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          128  CTCGAG               C^TCGAG
XhoII         758  GGATCT               [GA]^GATC[CT]
XmaCI          90  CCCGGG               C^CCGGG
XmaI           90  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AhdI         1410  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1727  GACAGTCGGTC         
AocI          404  CCTTAGG              CC^T[ACGT]AGG
             1174  CCTGAGG             
AspEI        1410  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1727  GACAGTCGGTC         
AspHI        1102  GTGCTC               G[AT]GC[AT]^C
             1347  GAGCAC              
AvaII         981  GGACC                G^G[AT]CC
             1511  GGACC               
BanI          160  GGTACC               G^G[CT][GA]CC
             1170  GGTGCC              
BanII         599  GGGCTC               G[GA]GC[CT]^C
              682  GAGCCC              
BsaAI         445  TACGTA               [CT]AC^GT[GA]
              522  CACGTA              
BsiHKAI      1102  GTGCTC               G[AT]GC[AT]^C
             1347  GAGCAC              
BsmFI         814  GGGAC                GGGAC(10/14)
             1443  GTCCC               
BspMI        1163  ACCTGC               ACCTGC(4/8)
             1167  GCAGGT              
Bsu36I        404  CCTTAGG              CC^T[ACGT]AGG
             1174  CCTGAGG             
DsaI          848  CCACGG               C^C[GA][CT]GG
             1301  CCGTGG              
Eam1105I     1410  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1727  GACAGTCGGTC         
Eco81I        404  CCTTAGG              CC^T[ACGT]AGG
             1174  CCTGAGG             
EcoNI         175  CCTCTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1446  CCTGGTCAAGG         
Tsp509I       142  AATT                 ^AATT
              267  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AfaI        AluI        AvaI        BbsI        
BbvI        BcnI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EaeI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
TspRI       TthHB8I     
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