Restriction map for pF1KB3755
 (Linear) (Six-base) MAPSORT of: KIBB3755.seq  Check: 2140  from: 1  to: 1770



KIBB3755.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1158    1770 
   Size:       1158     612
 
AatII G_ACGT'C

Cuts at:      0    1328    1770 
   Size:       1328     442
 
Acc65I G'GTAC_C

Cuts at:      0     160    1770 
   Size:        160    1610
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316    1426    1770 
   Size:        316    1110     344

  Fragments arranged by size: 

                1110     344     316
 
AflII C'TTAA_G

Cuts at:      0     321    1770 
   Size:        321    1449
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1415    1732    1770 
   Size:       1415     317      38

  Fragments arranged by size: 

                1415     317      38
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     633     665    1033    1065    1770 
   Size:        633      32     368      32     705

  Fragments arranged by size: 

                 705     633     368      32      32
 
AvaI C'yCGr_G

Cuts at:      0      90     128    1756    1770 
   Size:         90      38    1628      14

  Fragments arranged by size: 

                1628      90      38      14
 
BanI G'GyrC_C

Cuts at:      0     160    1170    1770 
   Size:        160    1010     600

  Fragments arranged by size: 

                1010     600     160
 
BanII G_rGCy'C

Cuts at:      0     603     686    1770 
   Size:        603      83    1084

  Fragments arranged by size: 

                1084     603      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     198     304    1414    1770 
   Size:        198     106    1110     356

  Fragments arranged by size: 

                1110     356     198     106
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     690     724    1770 
   Size:        690      34    1046

  Fragments arranged by size: 

                1046     690      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      53    1770 
   Size:         53    1717
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1387    1770 
   Size:       1387     383
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1418    1770 
   Size:       1418     352
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     774    1057    1770 
   Size:        137     637     283     713

  Fragments arranged by size: 

                 713     637     283     137
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1090    1770 
   Size:       1090     680
 
BsaAI yAC'GTr

Cuts at:      0     447     524    1770 
   Size:        447      77    1246

  Fragments arranged by size: 

                1246     447      77
 
BsaHI Gr'CG_yC

Cuts at:      0    1325    1770 
   Size:       1325     445
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      34      55      64      85     633     663    1033    1057 
   Size:         34      21       9      21     548      30     370      24

Cuts at:   1057    1063    1087    1315    1345    1770 
   Size:          6      24     228      30     425

  Fragments arranged by size: 

                 548     425     370     228      34      30      30      24
                  24      21      21       9       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      42     138    1041    1075    1192    1195    1323    1770 
   Size:         42      96     903      34     117       3     128     447

  Fragments arranged by size: 

                 903     447     128     117      96      42      34       3
            
 
BseYI C'CCAG_C

Cuts at:      0     964    1185    1770 
   Size:        964     221     585

  Fragments arranged by size: 

                 964     585     221
 
BsiHKAI G_wGCw'C

Cuts at:      0    1106    1351    1770 
   Size:       1106     245     419

  Fragments arranged by size: 

                1106     419     245
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1072    1770 
   Size:       1072     698
 
Bsp1286I G_dGCh'C

Cuts at:      0     603     686    1106    1351    1770 
   Size:        603      83     420     245     419

  Fragments arranged by size: 

                 603     420     419     245      83
 
BspHI T'CATG_A

Cuts at:      0     389    1770 
   Size:        389    1381
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1158    1172    1770 
   Size:       1158      14     598

  Fragments arranged by size: 

                1158     598      14
 
BsrBI CCG'CTC

Cuts at:      0     519    1770 
   Size:        519    1251
 
BsrDI GCAATG_nn'

Cuts at:      0     462    1770 
   Size:        462    1308
 
BsrFI r'CCGG_y

Cuts at:      0     553    1770 
   Size:        553    1217
 
BssHII G'CGCG_C

Cuts at:      0    1749    1770 
   Size:       1749      21
 
BssSI C'ACGA_G

Cuts at:      0     510    1770 
   Size:        510    1260
 
BstEII G'GTnAC_C

Cuts at:      0     240    1770 
   Size:        240    1530
 
BstYI r'GATC_y

Cuts at:      0     758    1770 
   Size:        758    1012
 
Bsu36I CC'TnA_GG

Cuts at:      0     405    1175    1770 
   Size:        405     770     595

  Fragments arranged by size: 

                 770     595     405
 
BtgI C'CryG_G

Cuts at:      0     848    1301    1770 
   Size:        848     453     469

  Fragments arranged by size: 

                 848     469     453
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     195     693    1770 
   Size:        195     498    1077

  Fragments arranged by size: 

                1077     498     195
 
BtsI GCAGTG_nn'

Cuts at:      0    1005    1770 
   Size:       1005     765
 
ClaI AT'CG_AT

Cuts at:      0     396    1770 
   Size:        396    1374
 
EaeI y'GGCC_r

Cuts at:      0     924    1352    1388    1770 
   Size:        924     428      36     382

  Fragments arranged by size: 

                 924     428     382      36
 
EarI CTCTTCn'nnn_

Cuts at:      0    1228    1770 
   Size:       1228     542
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1035    1770 
   Size:       1035     735
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     316     774    1057    1426    1770 
   Size:        137     179     458     283     369     344

  Fragments arranged by size: 

                 458     369     344     283     179     137
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     179    1450    1770 
   Size:        179    1271     320

  Fragments arranged by size: 

                1271     320     179
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     552     584    1057    1089    1315    1347    1401    1433 
   Size:        552      32     473      32     226      32      54      32

Cuts at:   1433    1718    1750    1770 
   Size:        285      32      20

  Fragments arranged by size: 

                 552     473     285     226      54      32      32      32
                  32      32      20
 
HincII GTy'rAC

Cuts at:      0     285     306    1329    1770 
   Size:        285      21    1023     441

  Fragments arranged by size: 

                1023     441     285      21
 
HindIII A'AGCT_T

Cuts at:      0    1108    1266    1276    1770 
   Size:       1108     158      10     494

  Fragments arranged by size: 

                1108     494     158      10
 
KpnI G_GTAC'C

Cuts at:      0     164    1770 
   Size:        164    1606
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     612     932     988    1079    1770 
   Size:        612     320      56      91     691

  Fragments arranged by size: 

                 691     612     320      91      56
 
MscI TGG'CCA

Cuts at:      0    1390    1770 
   Size:       1390     380
 
MslI CAynn'nnrTG

Cuts at:      0     361     388     736    1770 
   Size:        361      27     348    1034

  Fragments arranged by size: 

                1034     361     348      27
 
MspA1I CmG'CkG

Cuts at:      0     136     294     929    1556    1770 
   Size:        136     158     635     627     214

  Fragments arranged by size: 

                 635     627     214     158     136
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     956    1770 
   Size:        956     814
 
PfoI T'CCnGG_A

Cuts at:      0      55     651    1770 
   Size:         55     596    1119

  Fragments arranged by size: 

                1119     596      55
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      87     119     633     665    1033    1065    1770 
   Size:         87      32     514      32     368      32     705

  Fragments arranged by size: 

                 705     514     368      87      32      32      32
 
PstI C_TGCA'G

Cuts at:      0     871    1169    1226    1770 
   Size:        871     298      57     544

  Fragments arranged by size: 

                 871     544     298      57
 
PvuII CAG'CTG

Cuts at:      0     136     294    1556    1770 
   Size:        136     158    1262     214

  Fragments arranged by size: 

                1262     214     158     136
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1228    1770 
   Size:       1228     542
 
SbfI CC_TGCA'GG

Cuts at:      0    1169    1770 
   Size:       1169     601
 
SfcI C'TryA_G

Cuts at:      0     867    1165    1222    1770 
   Size:        867     298      57     548

  Fragments arranged by size: 

                 867     548     298      57
 
SmaI CCC'GGG

Cuts at:      0      92    1770 
   Size:         92    1678
 
SmlI C'TyrA_G

Cuts at:      0     128     321    1105    1770 
   Size:        128     193     784     665

  Fragments arranged by size: 

                 784     665     193     128
 
SnaBI TAC'GTA

Cuts at:      0     447    1770 
   Size:        447    1323
 
StuI AGG'CCT

Cuts at:      0    1765    1770 
   Size:       1765       5
 
StyI C'CwwG_G

Cuts at:      0     236    1770 
   Size:        236    1534
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     556    1722    1770 
   Size:        556    1166      48

  Fragments arranged by size: 

                1166     556      48
 
TatI w'GTAC_w

Cuts at:      0      19    1770 
   Size:         19    1751
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      55      87    1770 
   Size:         55      32    1683

  Fragments arranged by size: 

                1683      55      32
 
XhoI C'TCGA_G

Cuts at:      0     128    1770 
   Size:        128    1642
 
XmaI C'CCGG_G

Cuts at:      0      90    1770 
   Size:         90    1680
 
ZraI GAC'GTC

Cuts at:      0    1326    1770 
   Size:       1326     444
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI    AflII     AhdI     AloI     AvaI
     BanI    BanII     BbsI     BcgI    BciVI     BglI     BplI     BpmI
    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     EaeI
     EarI     EciI  Eco57MI    EcoNI    Hin4I   HincII  HindIII     KpnI
     MmeI     MscI     MslI   MspA1I    PflMI     PfoI     PpiI     PstI
    PvuII     SapI     SbfI     SfcI     SmaI     SmlI    SnaBI     StuI
     StyI    TaqII     TatI     TstI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI   AflIII     AgeI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaBI    BsaWI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BsrGI   BstAPI    BstBI    BstXI
  BstZ17I    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI
     KasI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XbaI     XcmI     XmnI
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