Restriction map for pF1KE0095
 (Circular) (Six-base) MAPSORT of: pF1KE0095.seq  Check: 2252  from: 1  to: 6896



pF1KE0095  6896 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3117    3117 
   Size:       6896
 
AatII G_ACGT'C

Cuts at:   1859    1859 
   Size:       6896
 
AccI GT'mk_AC

Cuts at:   2378    3934    2378 
   Size:       1556    5340
 
Acc65I G'GTAC_C

Cuts at:   3912    3912 
   Size:       6896
 
AclI AA'CG_TT

Cuts at:   6736    6736 
   Size:       6896
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    375    1255    1709    2322    4721    5153    5900    6168     375 
   Size:        880     454     613    2399     432     747     268    1103

  Fragments arranged by size: 

                2399    1103     880     747     613     454     432     268
            
 
AfeI AGC'GCT

Cuts at:    802     802 
   Size:       6896
 
AflIII A'CryG_T

Cuts at:   2663    2738    3776    3800    4248    5352    6329    2663 
   Size:         75    1038      24     448    1104     977    3230

  Fragments arranged by size: 

                3230    1104    1038     977     448      75      24
 
AgeI A'CCGG_T

Cuts at:   3742    5284    3742 
   Size:       1542    5354
 
AhdI GACnn_n'nnGTC

Cuts at:   1860    1860 
   Size:       6896
 
AleI CACnn'nnGTG

Cuts at:   1715    1823    2159    2452    2638    1715 
   Size:        108     336     293     186    5973

  Fragments arranged by size: 

                5973     336     293     186     108
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    173     173 
   Size:       6896
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1459    1491    1459 
   Size:         32    6864
 
AlwNI CAG_nnn'CTG

Cuts at:    312     323    1230    1575    2807    3789    4201    5768     312 
   Size:         11     907     345    1232     982     412    1567    1440

  Fragments arranged by size: 

                1567    1440    1232     982     907     412     345      11
            
 
ApaI G_GGCC'C

Cuts at:    704    3881     704 
   Size:       3177    3719
 
ApaLI G'TGCA_C

Cuts at:   1560    5666    1560 
   Size:       4106    2790
 
ApoI r'AATT_y

Cuts at:   1799    3900    6249    6505    1799 
   Size:       2101    2349     256    2190

  Fragments arranged by size: 

                2349    2190    2101     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6896
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6896
 
AvaI C'yCGr_G

Cuts at:    135     173     244     277     491     684     690    1340    1677 
   Size:         38      71      33     214     193       6     650     337

Cuts at:   1677    2827    3916     135 
   Size:       1150    1089    3115

  Fragments arranged by size: 

                3115    1150    1089     650     337     214     193      71
                  38      33       6
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   3731    3764    3731 
   Size:         33    6863
 
BamHI G'GATC_C

Cuts at:   3921    3921 
   Size:       6896
 
BanI G'GyrC_C

Cuts at:    162     168     495     634     724    2392    2952    3912    4326 
   Size:          6     327     139      90    1668     560     960     414

Cuts at:   4326    4574    4609     162 
   Size:        248      35    2449

  Fragments arranged by size: 

                2449    1668     960     560     414     327     248     139
                  90      35       6
 
BanII G_rGCy'C

Cuts at:    133     253     691     704    1598    2930    3293    3644    3881 
   Size:        120     438      13     894    1332     363     351     237

Cuts at:   3881    3910    4940     133 
   Size:         29    1030    2089

  Fragments arranged by size: 

                2089    1332    1030     894     438     363     351     237
                 120      29      13
 
BbeI G_GCGC'C

Cuts at:    166     172     499     638     728    4330    4578     166 
   Size:          6     327     139      90    3602     248    2484

  Fragments arranged by size: 

                3602    2484     327     248     139      90       6
 
BbsI GAAGACnn'nnnn_

Cuts at:    160    2210    2412     160 
   Size:       2050     202    4644

  Fragments arranged by size: 

                4644    2050     202
 
BbvCI CC'TCA_GC

Cuts at:   1307    1307 
   Size:       6896
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1260    1294    1802    1836    1260 
   Size:         34     508      34    6320

  Fragments arranged by size: 

                6320     508      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4794    5555    6896    4794 
   Size:        761    1341    4794

  Fragments arranged by size: 

                4794    1341     761
 
BclI T'GATC_A

Cuts at:   4416    4416 
   Size:       6896
 
BglI GCCn_nnn'nGGC

Cuts at:    137     928    2401     137 
   Size:        791    1473    4632

  Fragments arranged by size: 

                4632    1473     791
 
BglII A'GATC_T

Cuts at:   4411    6448    4411 
   Size:       2037    4859
 
BlpI GC'TnA_GC

Cuts at:    355    1162    2135    2476    2854    4008     355 
   Size:        807     973     341     378    1154    3243

  Fragments arranged by size: 

                3243    1154     973     807     378     341
 
Bme1580I G_kGCm'C

Cuts at:    704    1564    2114    2459    3040    3681    3881    4521    4614 
   Size:        860     550     345     581     641     200     640      93

Cuts at:   4614    5670     704 
   Size:       1056    1930

  Fragments arranged by size: 

                1930    1056     860     641     640     581     550     345
                 200      93
 
BmgBI CAC'GTC

Cuts at:   2316    3249    2316 
   Size:        933    5963
 
BmrI ACTGGGnnnn_n'

Cuts at:   1551    2136    3027    4108    4189    4524    6668    1551 
   Size:        585     891    1081      81     335    2144    1779

  Fragments arranged by size: 

                2144    1779    1081     891     585     335      81
 
BmtI G_CTAG'C

Cuts at:   1882    1882 
   Size:       6896
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1767    5263    1767 
   Size:       3496    3400
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    607     646     805     889    2562    2664    3388    3484    3832 
   Size:         39     159      84    1673     102     724      96     348

Cuts at:   3832     607 
   Size:       3671

  Fragments arranged by size: 

                3671    1673     724     348     159     102      96      84
                  39
 
Bpu10I CC'TnA_GC

Cuts at:   1307    3041    1307 
   Size:       1734    5162
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1987    4072    4951    5443    5741    1987 
   Size:       2085     879     492     298    3142

  Fragments arranged by size: 

                3142    2085     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1317    1330    1840    2014    6600    1317 
   Size:         13     510     174    4586    1613

  Fragments arranged by size: 

                4586    1613     510     174      13
 
BsaAI yAC'GTr

Cuts at:   1642    2672    2748    2937    3803    4249    1642 
   Size:       1030      76     189     866     446    4289

  Fragments arranged by size: 

                4289    1030     866     446     189      76
 
BsaBI GATnn'nnATC

Cuts at:      6     952    1489    2449    3634    4410    6447       6 
   Size:        946     537     960    1185     776    2037     455

  Fragments arranged by size: 

                2037    1185     960     946     776     537     455
 
BsaHI Gr'CG_yC

Cuts at:    163     169     496     635     725    1552    1856    2282    3076 
   Size:          6     327     139      90     827     304     426     794

Cuts at:   3076    4327    4575    5277    6773     163 
   Size:       1251     248     702    1496     286

  Fragments arranged by size: 

                1496    1251     827     794     702     426     327     304
                 286     248     139      90       6
 
BsaWI w'CCGG_w

Cuts at:   3742    4085    4606    5284    5558    5705    6116    3742 
   Size:        343     521     678     274     147     411    4522

  Fragments arranged by size: 

                4522     678     521     411     343     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    778     808    3013    3043     778 
   Size:         30    2205      30    4631

  Fragments arranged by size: 

                4631    2205      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    604    3193    3202    3211     604 
   Size:       2589       9       9    4289

  Fragments arranged by size: 

                4289    2589       9       9
 
BseYI C'CCAG_C

Cuts at:   2691    3122    4321    5079    5656    6152    2691 
   Size:        431    1199     758     577     496    3435

  Fragments arranged by size: 

                3435    1199     758     577     496     431
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1254    1275    1550    2025    3100    3211    1254 
   Size:         21     275     475    1075     111    4939

  Fragments arranged by size: 

                4939    1075     475     275     111      21
 
BsiEI CG_ry'CG

Cuts at:    104     444     617     935    2385    2762    3756    4438    4484 
   Size:        340     173     318    1450     377     994     682      46

Cuts at:   4484    5692     104 
   Size:       1208    1308

  Fragments arranged by size: 

                1450    1308    1208     994     682     377     340     318
                 173      46
 
BsiHKAI G_wGCw'C

Cuts at:    860    1163    1175    1564    1598    2012    2315    2714    2912 
   Size:        303      12     389      34     414     303     399     198

Cuts at:   2912    2930    2942    3293    3611    3910    4688    4878    5670 
   Size:         18      12     351     318     299     778     190     792

Cuts at:   5670     860 
   Size:       2086

  Fragments arranged by size: 

                2086     792     778     414     399     389     351     318
                 303     303     299     198     190      34      18      12
                  12
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1680    1680 
   Size:       6896
 
Bsp1286I G_dGCh'C

Cuts at:    133     253     691     704     860    1163    1175    1564    1598 
   Size:        120     438      13     156     303      12     389      34

Cuts at:   1598    2012    2114    2315    2459    2714    2912    2930    2942 
   Size:        414     102     201     144     255     198      18      12

Cuts at:   2942    3040    3293    3611    3644    3681    3881    3910    4521 
   Size:         98     253     318      33      37     200      29     611

Cuts at:   4521    4614    4688    4878    4940    5670     133 
   Size:         93      74     190      62     730    1359

  Fragments arranged by size: 

                1359     730     611     438     414     389     318     303
                 255     253     201     200     198     190     156     144
                 120     102      98      93      74      62      37      34
                  33      29      18      13      12      12
 
BspEI T'CCGG_A

Cuts at:   6116    6116 
   Size:       6896
 
BspHI T'CATG_A

Cuts at:   1580    6072    1580 
   Size:       4492    2404
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2364    2696    2993    3117    3122    3946    4160    4462    4843 
   Size:        332     297     124       5     824     214     302     381

Cuts at:   4843    2364 
   Size:       4417

  Fragments arranged by size: 

                4417     824     381     332     302     297     214     124
                   5
 
BsrBI CCG'CTC

Cuts at:     50     194     359     401     594     758     789     903    3256 
   Size:        144     165      42     193     164      31     114    2353

Cuts at:   3256    3276    3885    5188    5242    6726    6891      50 
   Size:         20     609    1303      54    1484     165      55

  Fragments arranged by size: 

                2353    1484    1303     609     193     165     165     164
                 144     114      55      54      42      31      20
 
BsrDI GCAATG_nn'

Cuts at:   3288    4808    3288 
   Size:       1520    5376
 
BsrFI r'CCGG_y

Cuts at:    165     455     631    3215    3742    4894    5075    5284     165 
   Size:        290     176    2584     527    1152     181     209    1777

  Fragments arranged by size: 

                2584    1777    1152     527     290     209     181     176
            
 
BssHII G'CGCG_C

Cuts at:    609     609 
   Size:       6896
 
BssSI C'ACGA_G

Cuts at:    859    5167    5525     859 
   Size:       4308     358    2230

  Fragments arranged by size: 

                4308    2230     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   4273    4273 
   Size:       6896
 
BstBI TT'CG_AA

Cuts at:   5257    5257 
   Size:       6896
 
BstEII G'GTnAC_C

Cuts at:    288    2991    3765     288 
   Size:       2703     774    3419

  Fragments arranged by size: 

                3419    2703     774
 
BstXI CCAn_nnnn'nTGG

Cuts at:    843    1281    1755    1890    2126    2161    2637    3249    3732 
   Size:        438     474     135     236      35     476     612     483

Cuts at:   3732     843 
   Size:       4007

  Fragments arranged by size: 

                4007     612     483     476     474     438     236     135
                  35
 
BstYI r'GATC_y

Cuts at:      1    1649    3921    4388    4411    4746    4992    6004    6090 
   Size:       1648    2272     467      23     335     246    1012      86

Cuts at:   6090    6102    6448       1 
   Size:         12     346     449

  Fragments arranged by size: 

                2272    1648    1012     467     449     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   2243    2243 
   Size:       6896
 
BtgI C'CryG_G

Cuts at:    258    1028    1430    3656     258 
   Size:        770     402    2226    3498

  Fragments arranged by size: 

                3498    2226     770     402
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1008    1182    2795    3321    3402    4846    5027    5190    1008 
   Size:        174    1613     526      81    1444     181     163    2714

  Fragments arranged by size: 

                2714    1613    1444     526     181     174     163      81
            
 
BtsI GCAGTG_nn'

Cuts at:   1163    1562    1710    1818    2563    3137    4139    1163 
   Size:        399     148     108     745     574    1002    3920

  Fragments arranged by size: 

                3920    1002     745     574     399     148     108
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   3038    3073    3038 
   Size:         35    6861
 
DraI TTT'AAA

Cuts at:   3895    3895 
   Size:       6896
 
DrdI GACnn_nn'nnGTC

Cuts at:   2989    4602    5460    2989 
   Size:       1613     858    4425

  Fragments arranged by size: 

                4425    1613     858
 
EaeI y'GGCC_r

Cuts at:    314     374     473     614     845     920     932    1037    1193 
   Size:         60      99     141     231      75      12     105     156

Cuts at:   1193    1619    2759    2918    3251    3560    3662    3722    4481 
   Size:        426    1140     159     333     309     102      60     759

Cuts at:   4481    4655    5046    5073    6418     314 
   Size:        174     391      27    1345     792

  Fragments arranged by size: 

                1345    1140     792     759     426     391     333     309
                 231     174     159     156     141     105     102      99
                  75      60      60      27      12
 
EagI C'GGCC_G

Cuts at:    614     932    2759    4481     614 
   Size:        318    1827    1722    3029

  Fragments arranged by size: 

                3029    1827    1722     318
 
EarI CTCTTCn'nnn_

Cuts at:   1603    2054    2431    2557    2974    4919    5129    1603 
   Size:        451     377     126     417    1945     210    3370

  Fragments arranged by size: 

                3370    1945     451     417     377     210     126
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    399     694     802    1170    2767    5104    5414    5560    6706 
   Size:        295     108     368    1597    2337     310     146    1146

Cuts at:   6706     399 
   Size:        589

  Fragments arranged by size: 

                2337    1597    1146     589     368     310     295     146
                 108
 
EcoICRI GAG'CTC

Cuts at:   1596    2928    3291    3908    1596 
   Size:       1332     363     617    4584

  Fragments arranged by size: 

                4584    1332     617     363
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    375     607     646     805     889    1255    1709    2322    2562 
   Size:        232      39     159      84     366     454     613     240

Cuts at:   2562    2664    3388    3484    3832    4721    5153    5900    6168 
   Size:        102     724      96     348     889     432     747     268

Cuts at:   6168     375 
   Size:       1103

  Fragments arranged by size: 

                1103     889     747     724     613     454     432     366
                 348     268     240     232     159     102      96      84
                  39
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3241    3241 
   Size:       6896
 
EcoO109I rG'GnC_Cy

Cuts at:    181     700    2685    3026    3877    3878    4035     181 
   Size:        519    1985     341     851       1     157    3042

  Fragments arranged by size: 

                3042    1985     851     519     341     157       1
 
EcoRI G'AATT_C

Cuts at:   1799    3900    1799 
   Size:       2101    4795
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4915    4915 
   Size:       6896
 
FspI TGC'GCA

Cuts at:   1144    1207    4677    1144 
   Size:         63    3470    3363

  Fragments arranged by size: 

                3470    3363      63
 
FspAI rTGC'GCAy

Cuts at:   1144    1144 
   Size:       6896
 
HaeII r_GCGC'y

Cuts at:    166     172     312     499     638     728     804    1082    2142 
   Size:          6     140     187     139      90      76     278    1060

Cuts at:   2142    4330    4578    5600    6579     166 
   Size:       2188     248    1022     979     483

  Fragments arranged by size: 

                2188    1060    1022     979     483     278     248     187
                 140     139      90      76       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    673     705    1334    1366    1846    1878    4738    4768    4770 
   Size:         32     629      32     480      32    2860      30       2

Cuts at:   4770    4800     673 
   Size:         30    2769

  Fragments arranged by size: 

                2860    2769     629     480      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   2661    2736    2766    3935    2661 
   Size:         75      30    1169    5622

  Fragments arranged by size: 

                5622    1169      75      30
 
KasI G'GCGC_C

Cuts at:    162     168     495     634     724    4326    4574     162 
   Size:          6     327     139      90    3602     248    2484

  Fragments arranged by size: 

                3602    2484     327     248     139      90       6
 
KpnI G_GTAC'C

Cuts at:   3916    3916 
   Size:       6896
 
MluI A'CGCG_T

Cuts at:   2738    6329    2738 
   Size:       3591    3305
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    755    5567    5751     755 
   Size:       4812     184    1900

  Fragments arranged by size: 

                4812    1900     184
 
MscI TGG'CCA

Cuts at:    316    1195    2920    3724    4657     316 
   Size:        879    1725     804     933    2555

  Fragments arranged by size: 

                2555    1725     933     879     804
 
MslI CAynn'nnrTG

Cuts at:    841     847     961    1027    1435    1715    1735    1792    1823 
   Size:          6     114      66     408     280      20      57      31

Cuts at:   1823    2159    2452    2470    2638    2935    3493    3775    4885 
   Size:        336     293      18     168     297     558     282    1110

Cuts at:   4885    5012     841 
   Size:        127    2725

  Fragments arranged by size: 

                2725    1110     558     408     336     297     293     282
                 280     168     127     114      66      57      31      20
                  18       6
 
MspA1I CmG'CkG

Cuts at:    260     323     354     462     479     909    1166    1374    1808 
   Size:         63      31     108      17     430     257     208     434

Cuts at:   1808    2891    3462    3810    4007    4321    4681    5694    5939 
   Size:       1083     571     348     197     314     360    1013     245

Cuts at:   5939     260 
   Size:       1217

  Fragments arranged by size: 

                1217    1083    1013     571     434     430     360     348
                 314     257     245     208     197     108      63      31
                  17
 
NaeI GCC'GGC

Cuts at:    167     457     633    5077     167 
   Size:        290     176    4444    1986

  Fragments arranged by size: 

                4444    1986     290     176
 
NarI GG'CG_CC

Cuts at:    163     169     496     635     725    4327    4575     163 
   Size:          6     327     139      90    3602     248    2484

  Fragments arranged by size: 

                3602    2484     327     248     139      90       6
 
NcoI C'CATG_G

Cuts at:   1430    1430 
   Size:       6896
 
NgoMIV G'CCGG_C

Cuts at:    165     455     631    5075     165 
   Size:        290     176    4444    1986

  Fragments arranged by size: 

                4444    1986     290     176
 
NheI G'CTAG_C

Cuts at:   1878    1878 
   Size:       6896
 
NotI GC'GGCC_GC

Cuts at:    932     932 
   Size:       6896
 
NspI r_CATG'y

Cuts at:   1149    1791    2667    3049    3780    3949    4449    5356    1149 
   Size:        642     876     382     731     169     500     907    2689

  Fragments arranged by size: 

                2689     907     876     731     642     500     382     169
            
 
PasI CC'CwG_GG

Cuts at:    720     720 
   Size:       6896
 
PciI A'CATG_T

Cuts at:   2663    3776    5352    2663 
   Size:       1113    1576    4207

  Fragments arranged by size: 

                4207    1576    1113
 
PflMI CCAn_nnn'nTGG

Cuts at:    323    1050    1978    3129    3789     323 
   Size:        727     928    1151     660    3430

  Fragments arranged by size: 

                3430    1151     928     727     660
 
PfoI T'CCnGG_A

Cuts at:   1331    1760    2578    1331 
   Size:        429     818    5649

  Fragments arranged by size: 

                5649     818     429
 
PmeI GTTT'AAAC

Cuts at:   3895    3895 
   Size:       6896
 
PmlI CAC'GTG

Cuts at:   2672    2748    2672 
   Size:         76    6820
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3436    3468    6035    6067    3436 
   Size:         32    2567      32    4265

  Fragments arranged by size: 

                4265    2567      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2685    3026    2685 
   Size:        341    6555
 
PshAI GACnn'nnGTC

Cuts at:    440     440 
   Size:       6896
 
PspOMI G'GGCC_C

Cuts at:    700    3877     700 
   Size:       3177    3719
 
PstI C_TGCA'G

Cuts at:    749    2570    2885    3170    3206    3943    4628     749 
   Size:       1821     315     285      36     737     685    3017

  Fragments arranged by size: 

                3017    1821     737     685     315     285      36
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6896
 
PvuII CAG'CTG

Cuts at:    909    2891    3462    3810    4321    4681     909 
   Size:       1982     571     348     511     360    3124

  Fragments arranged by size: 

                3124    1982     571     511     360     348
 
RsrII CG'GwC_CG

Cuts at:   5091    5091 
   Size:       6896
 
SacI G_AGCT'C

Cuts at:   1598    2930    3293    3910    1598 
   Size:       1332     363     617    4584

  Fragments arranged by size: 

                4584    1332     617     363
 
SacII CC_GC'GG

Cuts at:    261     261 
   Size:       6896
 
SalI G'TCGA_C

Cuts at:   3933    3933 
   Size:       6896
 
SapI GCTCTTCn'nnn_

Cuts at:   1603    2557    2974    4919    5129    1603 
   Size:        954     417    1945     210    3370

  Fragments arranged by size: 

                3370    1945     954     417     210
 
SbfI CC_TGCA'GG

Cuts at:   3943    3943 
   Size:       6896
 
ScaI AGT'ACT

Cuts at:   2365    2833    6413    2365 
   Size:        468    3580    2848

  Fragments arranged by size: 

                3580    2848     468
 
SexAI A'CCwGG_T

Cuts at:   2168    3782    2168 
   Size:       1614    5282
 
SfcI C'TryA_G

Cuts at:     33     745    2566    2769    2841    2881    3166    3202    3939 
   Size:        712    1821     203      72      40     285      36     737

Cuts at:   3939    4624    5617    5808      33 
   Size:        685     993     191    1121

  Fragments arranged by size: 

                1821    1121     993     737     712     685     285     203
                 191      72      40      36
 
SfoI GGC'GCC

Cuts at:    164     170     497     636     726    4328    4576     164 
   Size:          6     327     139      90    3602     248    2484

  Fragments arranged by size: 

                3602    2484     327     248     139      90       6
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6896
 
SgrAI Cr'CCGG_yG

Cuts at:    165    3742     165 
   Size:       3577    3319
 
SmaI CCC'GGG

Cuts at:    137     175     246     279     493    3918     137 
   Size:         38      71      33     214    3425    3115

  Fragments arranged by size: 

                3425    3115     214      71      38      33
 
SmlI C'TyrA_G

Cuts at:   2002    4051    4966    5458    5720    2002 
   Size:       2049     915     492     262    3178

  Fragments arranged by size: 

                3178    2049     915     492     262
 
SnaBI TAC'GTA

Cuts at:   1642    1642 
   Size:       6896
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6896
 
SphI G_CATG'C

Cuts at:   1149    3049    3949    4449    1149 
   Size:       1900     900     500    3596

  Fragments arranged by size: 

                3596    1900     900     500
 
StuI AGG'CCT

Cuts at:   3208    3208 
   Size:       6896
 
StyI C'CwwG_G

Cuts at:   1430    1653    1830    1928    2177    2210    2510    3053    3242 
   Size:        223     177      98     249      33     300     543     189

Cuts at:   3242    4030    4384    1430 
   Size:        788     354    3942

  Fragments arranged by size: 

                3942     788     543     354     300     249     223     189
                 177      98      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1273    1309    2059    2149    2294    2399    4479    4612    5071 
   Size:         36     750      90     145     105    2080     133     459

Cuts at:   5071    5280    1273 
   Size:        209    2889

  Fragments arranged by size: 

                2889    2080     750     459     209     145     133     105
                  90      36
 
TatI w'GTAC_w

Cuts at:   1815    2363    2831    3596    6411    1815 
   Size:        548     468     765    2815    2300

  Fragments arranged by size: 

                2815    2300     765     548     468
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1284    1316    6413    6445    1284 
   Size:         32    5097      32    1735

  Fragments arranged by size: 

                5097    1735      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   4693    4693 
   Size:       6896
 
XbaI T'CTAG_A

Cuts at:   3927    3927 
   Size:       6896
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1894    2688    3579    4391    1894 
   Size:        794     891     812    4399

  Fragments arranged by size: 

                4399     891     812     794
 
XmaI C'CCGG_G

Cuts at:    135     173     244     277     491    3916     135 
   Size:         38      71      33     214    3425    3115

  Fragments arranged by size: 

                3425    3115     214      71      38      33
 
ZraI GAC'GTC

Cuts at:   1857    1857 
   Size:       6896
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

    AflII     AscI    AvrII    BfrBI    BsiWI     BsmI    BsrGI  BstZ17I
     ClaI   DraIII    EcoRV     FseI  HindIII     HpaI     MfeI     NdeI
     NruI     NsiI     PacI     PsiI    PspXI     PsrI    SanDI     SfiI
     SrfI     SspI     SwaI     XhoI     XmnI
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