Restriction map for pF1KE0081
##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        AvrII       BamHI       
BbeI        BfrI        BlnI        BsaBI       BseAI       
BsiWI       BsmBI       BsmI        BspEI       BssHII      
Bst1107I    BstBI       BstZ17I     CpoI        Eco47III    
EcoRV       EcoT22I     FseI        HaeII       HpaI        
KasI        KpnI        MamI        MfeI        MluI        
MroI        MunI        NarI        NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SalI        SbfI        SfiI        SfoI        SfuI        
SnaBI       SpeI        Sse8387I    SwaI        Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI         2374  GTCTAC               GT^[AC][GT]AC
AflIII       2219  ACATGT               A^C[GA][CT]GT
AgeI          793  ACCGGT               A^CCGGT
ApaI         1569  GGGCCC               GGGCC^C
BanI           24  GGTGCC               G^G[CT][GA]CC
BbrPI        1999  CACGTG               CAC^GTG
BbvCI         465  GCTGAGG              CCTCAGC(-5/-2)
BcgI         3980  GCACCGTGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          845  TGATCA               T^GATCA
BglI         4114  GCCCTCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1999  CACGTG               [CT]AC^GT[GA]
BsaWI         793  ACCGGT               [AT]^CCGG[AT]
Bsp1407I     1061  TGTACA               T^GTACA
BspDI        3901  ATCGAT               AT^CGAT
BspLU11I     2219  ACATGT               A^CATGT
BsrGI        1061  TGTACA               T^GTACA
BstAPI        907  GCATTTATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2064  GGTGACC              G^GT[ACGT]ACC
BstPI        2064  GGTGACC              G^GT[ACGT]ACC
ClaI         3901  ATCGAT               AT^CGAT
DraI         3226  TTTAAA               TTT^AAA
DraIII       3502  CACGAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          379  GACAAAAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         4072  CGGCCG               C^GGCCG
EclXI        4072  CGGCCG               C^GGCCG
Eco52I       4072  CGGCCG               C^GGCCG
EcoO65I      2064  GGTGACC              G^GT[ACGT]ACC
EcoRI        1326  GAATTC               G^AATTC
FbaI          845  TGATCA               T^GATCA
HincII       3241  GTCAAC               GT[CT]^[GA]AC
HindII       3241  GTCAAC               GT[CT]^[GA]AC
KspI         3495  CCGCGG               CCGC^GG
MaeII        2000  ACGT                 A^CGT
NaeI         2750  GCCGGC               GCC^GGC
NdeI         1790  CATATG               CA^TATG
NgoMIV       2750  GCCGGC               G^CCGGC
PaeR7I       1690  CTCGAG               C^TCGAG
PinAI         793  ACCGGT               A^CCGGT
PmaCI        1999  CACGTG               CAC^GTG
PmlI         1999  CACGTG               CAC^GTG
PpuMI        1841  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI        2052  GACGGTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         3027  GAGCTC               GAGCT^C
SacII        3495  CCGCGG               CCGC^GG
SexAI        1592  ACCTGGT              A^CC[AT]GGT
SgrAI         792  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         1462  CCCGGG               CCC^GGG
SspBI        1061  TGTACA               T^GTACA
XhoI         1690  CTCGAG               C^TCGAG
XmaCI        1462  CCCGGG               C^CCGGG
XmaI         1462  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII        1936  CGCG                 CG^CG
             3496  CGCG                
AcyI         1993  GACGCC               G[GA]^CG[CT]C
             3261  GGCGTC              
AhdI         1975  GACCCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3637  GACTTGCGGTC         
Asp700I      1356  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2760  GAACTGCTTC          
AspEI        1975  GACCCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3637  GACTTGCGGTC         
AviII         853  TGCGCA               TGC^GCA
             3090  TGCGCA              
BciVI         898  GGATAC               GTATCC(6/5)
             3329  GGATAC              
BglII        1958  AGATCT               A^GATCT
             3737  AGATCT              
BlpI          979  GCTCAGC              GC^T[ACGT]AGC
             2478  GCTGAGC             
Bpu1102I      979  GCTCAGC              GC^T[ACGT]AGC
             2478  GCTGAGC             
BsaHI        1993  GACGCC               G[GA]^CG[CT]C
             3261  GGCGTC              
BsrBI         304  CCGCTC               CCGCTC(-3/-3)
             1885  GAGCGG              
BssSI        2661  CACGAG               CACGAG(-5/-1)
               46  CTCGTG              
BstUI        1936  CGCG                 CG^CG
             3496  CGCG                
CelII         979  GCTCAGC              GC^T[ACGT]AGC
             2478  GCTGAGC             
DraII        1841  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1889  GGGGCCT             
Eam1105I     1975  GACCCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3637  GACTTGCGGTC         
EcoNI        3749  CCTTGGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3765  CCTGACCAAGG         
EcoO109I     1841  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1889  GGGGCCT             
FspI          853  TGCGCA               TGC^GCA
             3090  TGCGCA              
Hin1I        1993  GACGCC               G[GA]^CG[CT]C
             3261  GGCGTC              
HindIII       318  AAGCTT               A^AGCTT
              443  AAGCTT              
MvnI         1936  CGCG                 CG^CG
             3496  CGCG                
SapI          577  GAAGAGC              GCTCTTC(1/4)
             2694  GCTCTTC             
ScaI          840  AGTACT               AGT^ACT
             2081  AGTACT              
SphI         1256  GCATGC               GCATG^C
             1503  GCATGC              
SspI          543  AATATT               AAT^ATT
              569  AATATT              
XmnI         1356  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2760  GAACTGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AspHI       AvaI        
AvaII       BalI        BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspHI       BspMI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        Eco81I      EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PstI        PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       
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