Restriction map for pF1KE0081
 (Linear) (Six-base) MAPSORT of: KIEE0081.seq  Check: 9024  from: 1  to: 4146



KIEE0081.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     859    4146 
   Size:        859    3287
 
AccI GT'mk_AC

Cuts at:      0    2375    4146 
   Size:       2375    1771
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431    1236    2448    3074    4146 
   Size:        431     805    1212     626    1072

  Fragments arranged by size: 

                1212    1072     805     626     431
 
AflIII A'CryG_T

Cuts at:      0    2219    4146 
   Size:       2219    1927
 
AgeI A'CCGG_T

Cuts at:      0     793    4146 
   Size:        793    3353
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1980    3642    4146 
   Size:       1980    1662     504

  Fragments arranged by size: 

                1980    1662     504
 
AleI CACnn'nnGTG

Cuts at:      0    4012    4146 
   Size:       4012     134
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     198     230    4146 
   Size:        198      32    3916

  Fragments arranged by size: 

                3916     198      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     255    1152    2274    2471    2823    4146 
   Size:        255     897    1122     197     352    1323

  Fragments arranged by size: 

                1323    1122     897     352     255     197
 
ApaI G_GGCC'C

Cuts at:      0    1573    4146 
   Size:       1573    2573
 
ApoI r'AATT_y

Cuts at:      0    1326    2089    4063    4146 
   Size:       1326     763    1974      83

  Fragments arranged by size: 

                1974    1326     763      83
 
AvaI C'yCGr_G

Cuts at:      0     535    1462    1540    1690    2502    4146 
   Size:        535     927      78     150     812    1644

  Fragments arranged by size: 

                1644     927     812     535     150      78
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    3322    3355    4146 
   Size:       3322      33     791

  Fragments arranged by size: 

                3322     791      33
 
BanI G'GyrC_C

Cuts at:      0      24    4146 
   Size:         24    4122
 
BanII G_rGCy'C

Cuts at:      0    1378    1573    1699    2503    2603    3031    3535    3581 
   Size:       1378     195     126     804     100     428     504      46

Cuts at:   3581    4146 
   Size:        565

  Fragments arranged by size: 

                1378     804     565     504     428     195     126     100
                  46
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1103    2041    2056    2442    3062    4146 
   Size:       1103     938      15     386     620    1084

  Fragments arranged by size: 

                1103    1084     938     620     386      15
 
BbvCI CC'TCA_GC

Cuts at:      0     466    4146 
   Size:        466    3680
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3969    4003    4146 
   Size:       3969      34     143

  Fragments arranged by size: 

                3969     143      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     892    3323    4146 
   Size:        892    2431     823

  Fragments arranged by size: 

                2431     892     823
 
BclI T'GATC_A

Cuts at:      0     845    4146 
   Size:        845    3301
 
BglI GCCn_nnn'nGGC

Cuts at:      0    4120    4146 
   Size:       4120      26
 
BglII A'GATC_T

Cuts at:      0    1958    3737    4146 
   Size:       1958    1779     409

  Fragments arranged by size: 

                1958    1779     409
 
BlpI GC'TnA_GC

Cuts at:      0     980    2479    4146 
   Size:        980    1499    1667

  Fragments arranged by size: 

                1667    1499     980
 
Bme1580I G_kGCm'C

Cuts at:      0      29    1482    1573    4146 
   Size:         29    1453      91    2573

  Fragments arranged by size: 

                2573    1453      91      29
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      30    4146 
   Size:         30    4116
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     628    1555    2517    4146 
   Size:        628     927     962    1629

  Fragments arranged by size: 

                1629     962     927     628
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     147     648    1351    1504    1510    1693    1795 
   Size:        140       7     501     703     153       6     183     102

Cuts at:   1795    2420    2989    3169    3652    4146 
   Size:        625     569     180     483     494

  Fragments arranged by size: 

                 703     625     569     501     494     483     183     180
                 153     140     102       7       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     466    3025    4146 
   Size:        466    2559    1121

  Fragments arranged by size: 

                2559    1121     466
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     222     408    2382    3650    4146 
   Size:        222     186    1974    1268     496

  Fragments arranged by size: 

                1974    1268     496     222     186
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1324    2418    2990    4146 
   Size:       1324    1094     572    1156

  Fragments arranged by size: 

                1324    1156    1094     572
 
BsaAI yAC'GTr

Cuts at:      0    2001    4146 
   Size:       2001    2145
 
BsaHI Gr'CG_yC

Cuts at:      0    1994    3262    4146 
   Size:       1994    1268     884

  Fragments arranged by size: 

                1994    1268     884
 
BsaWI w'CCGG_w

Cuts at:      0     793    4146 
   Size:        793    3353
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     336     366    1813    1843    4146 
   Size:        336      30    1447      30    2303

  Fragments arranged by size: 

                2303    1447     336      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     651    1444    1690    2035    2353    2356    2605    2628 
   Size:        651     793     246     345     318       3     249      23

Cuts at:   2628    4146 
   Size:       1518

  Fragments arranged by size: 

                1518     793     651     345     318     249     246      23
                   3
 
BseYI C'CCAG_C

Cuts at:      0     248    1408    1733    4146 
   Size:        248    1160     325    2413

  Fragments arranged by size: 

                2413    1160     325     248
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1729    2668    3963    4146 
   Size:       1729     939    1295     183

  Fragments arranged by size: 

                1729    1295     939     183
 
BsiEI CG_ry'CG

Cuts at:      0    1953    2057    4075    4146 
   Size:       1953     104    2018      71

  Fragments arranged by size: 

                2018    1953     104      71
 
BsiHKAI G_wGCw'C

Cuts at:      0    1820    3031    3989    4146 
   Size:       1820    1211     958     157

  Fragments arranged by size: 

                1820    1211     958     157
 
Bsp1286I G_dGCh'C

Cuts at:      0      29    1378    1482    1573    1699    1820    2503    2603 
   Size:         29    1349     104      91     126     121     683     100

Cuts at:   2603    3031    3535    3581    3989    4146 
   Size:        428     504      46     408     157

  Fragments arranged by size: 

                1349     683     504     428     408     157     126     121
                 104     100      91      46      29
 
BspHI T'CATG_A

Cuts at:      0     308     956    3155    4146 
   Size:        308     648    2199     991

  Fragments arranged by size: 

                2199     991     648     308
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     859    1973    3113    4146 
   Size:        859    1114    1140    1033

  Fragments arranged by size: 

                1140    1114    1033     859
 
BsrBI CCG'CTC

Cuts at:      0     306    1887    4146 
   Size:        306    1581    2259

  Fragments arranged by size: 

                2259    1581     306
 
BsrDI GCAATG_nn'

Cuts at:      0     521    2535    2555    3860    3992    4053    4146 
   Size:        521    2014      20    1305     132      61      93

  Fragments arranged by size: 

                2014    1305     521     132      93      61      20
 
BsrFI r'CCGG_y

Cuts at:      0     793    2750    2981    4146 
   Size:        793    1957     231    1165

  Fragments arranged by size: 

                1957    1165     793     231
 
BsrGI T'GTAC_A

Cuts at:      0    1061    4146 
   Size:       1061    3085
 
BssSI C'ACGA_G

Cuts at:      0      46    2661    4146 
   Size:         46    2615    1485

  Fragments arranged by size: 

                2615    1485      46
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     913    4146 
   Size:        913    3233
 
BstEII G'GTnAC_C

Cuts at:      0    2064    4146 
   Size:       2064    2082
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2286    2398    2907    3043    3927    4146 
   Size:       2286     112     509     136     884     219

  Fragments arranged by size: 

                2286     884     509     219     136     112
 
BstYI r'GATC_y

Cuts at:      0     560    1958    3669    3708    3737    4146 
   Size:        560    1398    1711      39      29     409

  Fragments arranged by size: 

                1711    1398     560     409      39      29
 
Bsu36I CC'TnA_GG

Cuts at:      0     149     768    2640    4146 
   Size:        149     619    1872    1506

  Fragments arranged by size: 

                1872    1506     619     149
 
BtgI C'CryG_G

Cuts at:      0     178     600    2537    2675    3495    3581    4146 
   Size:        178     422    1937     138     820      86     565

  Fragments arranged by size: 

                1937     820     565     422     178     138      86
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      86     842    4146 
   Size:         86     756    3304

  Fragments arranged by size: 

                3304     756      86
 
BtsI GCAGTG_nn'

Cuts at:      0     902    1346    1821    3278    4146 
   Size:        902     444     475    1457     868

  Fragments arranged by size: 

                1457     902     868     475     444
 
ClaI AT'CG_AT

Cuts at:      0    3902    4146 
   Size:       3902     244
 
DraI TTT'AAA

Cuts at:      0    3228    4146 
   Size:       3228     918
 
DraIII CAC_nnn'GTG

Cuts at:      0    3507    4146 
   Size:       3507     639
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     385    4146 
   Size:        385    3761
 
EaeI y'GGCC_r

Cuts at:      0    1187    2097    2276    3403    3498    4072    4121    4146 
   Size:       1187     910     179    1127      95     574      49      25

  Fragments arranged by size: 

                1187    1127     910     574     179      95      49      25
            
 
EagI C'GGCC_G

Cuts at:      0    4072    4146 
   Size:       4072      74
 
EarI CTCTTCn'nnn_

Cuts at:      0       4      39     572    2285    2701    3568    4146 
   Size:          4      35     533    1713     416     867     578

  Fragments arranged by size: 

                1713     867     578     533     416      35       4
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3556    4146 
   Size:       3556     590
 
EcoICRI GAG'CTC

Cuts at:      0    3029    4146 
   Size:       3029    1117
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     147     431     648    1236    1351    1504    1510 
   Size:        140       7     284     217     588     115     153       6

Cuts at:   1510    1693    1795    2420    2448    2989    3074    3169    3652 
   Size:        183     102     625      28     541      85      95     483

Cuts at:   3652    4146 
   Size:        494

  Fragments arranged by size: 

                 625     588     541     494     483     284     217     183
                 153     140     115     102      95      85      28       7
                   6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3753    3769    4146 
   Size:       3753      16     377

  Fragments arranged by size: 

                3753     377      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1842    1890    4146 
   Size:       1842      48    2256

  Fragments arranged by size: 

                2256    1842      48
 
EcoRI G'AATT_C

Cuts at:      0    1326    4146 
   Size:       1326    2820
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3564    4146 
   Size:       3564     582
 
FspI TGC'GCA

Cuts at:      0     855    3092    4146 
   Size:        855    2237    1054

  Fragments arranged by size: 

                2237    1054     855
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     764     796    1966    1998    2888    2920    3628    3660 
   Size:        764      32    1170      32     890      32     708      32

Cuts at:   3660    4146 
   Size:        486

  Fragments arranged by size: 

                1170     890     764     708     486      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    3243    4146 
   Size:       3243     903
 
HindIII A'AGCT_T

Cuts at:      0     318     443    4146 
   Size:        318     125    3703

  Fragments arranged by size: 

                3703     318     125
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     649    1973    2093    2347    2471    4146 
   Size:        649    1324     120     254     124    1675

  Fragments arranged by size: 

                1675    1324     649     254     124     120
 
MscI TGG'CCA

Cuts at:      0    1189    2099    2278    3405    4123    4146 
   Size:       1189     910     179    1127     718      23

  Fragments arranged by size: 

                1189    1127     910     718     179      23
 
MslI CAynn'nnrTG

Cuts at:      0    1018    1777    2623    2905    2953    3041    3346    3806 
   Size:       1018     759     846     282      48      88     305     460

Cuts at:   3806    3925    4012    4146 
   Size:        119      87     134

  Fragments arranged by size: 

                1018     846     759     460     305     282     134     119
                  88      87      48
 
MspA1I CmG'CkG

Cuts at:      0     169     709    1195    1754    2076    2398    2589    2714 
   Size:        169     540     486     559     322     322     191     125

Cuts at:   2714    2787    3258    3497    3852    4146 
   Size:         73     471     239     355     294

  Fragments arranged by size: 

                 559     540     486     471     355     322     322     294
                 239     191     169     125      73
 
NaeI GCC'GGC

Cuts at:      0    2752    4146 
   Size:       2752    1394
 
NcoI C'CATG_G

Cuts at:      0     600    2537    3581    4146 
   Size:        600    1937    1044     565

  Fragments arranged by size: 

                1937    1044     600     565
 
NdeI CA'TA_TG

Cuts at:      0    1791    4146 
   Size:       1791    2355
 
NgoMIV G'CCGG_C

Cuts at:      0    2750    4146 
   Size:       2750    1396
 
NspI r_CATG'y

Cuts at:      0    1260    1507    2223    4146 
   Size:       1260     247     716    1923

  Fragments arranged by size: 

                1923    1260     716     247
 
PasI CC'CwG_GG

Cuts at:      0    3396    4146 
   Size:       3396     750
 
PciI A'CATG_T

Cuts at:      0    2219    4146 
   Size:       2219    1927
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     255     606    3131    3412    3507    4035    4146 
   Size:        255     351    2525     281      95     528     111

  Fragments arranged by size: 

                2525     528     351     281     255     111      95
 
PfoI T'CCnGG_A

Cuts at:      0     563    1367    1670    4146 
   Size:        563     804     303    2476

  Fragments arranged by size: 

                2476     804     563     303
 
PmlI CAC'GTG

Cuts at:      0    2001    4146 
   Size:       2001    2145
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3295    3327    4146 
   Size:       3295      32     819

  Fragments arranged by size: 

                3295     819      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1842    4146 
   Size:       1842    2304
 
PshAI GACnn'nnGTC

Cuts at:      0    2056    4146 
   Size:       2056    2090
 
PsiI TTA'TAA

Cuts at:      0      98    4146 
   Size:         98    4048
 
PspOMI G'GGCC_C

Cuts at:      0    1569    4146 
   Size:       1569    2577
 
PspXI vC'TCGA_Gb

Cuts at:      0    1690    4146 
   Size:       1690    2456
 
PstI C_TGCA'G

Cuts at:      0     874    1341    2081    3257    3338    4146 
   Size:        874     467     740    1176      81     808

  Fragments arranged by size: 

                1176     874     808     740     467      81
 
PvuII CAG'CTG

Cuts at:      0     169     709    2076    2398    2589    2787    3258    3852 
   Size:        169     540    1367     322     191     198     471     594

Cuts at:   3852    4146 
   Size:        294

  Fragments arranged by size: 

                1367     594     540     471     322     294     198     191
                 169
 
SacI G_AGCT'C

Cuts at:      0    3031    4146 
   Size:       3031    1115
 
SacII CC_GC'GG

Cuts at:      0    3498    4146 
   Size:       3498     648
 
SapI GCTCTTCn'nnn_

Cuts at:      0     572    2701    4146 
   Size:        572    2129    1445

  Fragments arranged by size: 

                2129    1445     572
 
ScaI AGT'ACT

Cuts at:      0     842    2083    4146 
   Size:        842    1241    2063

  Fragments arranged by size: 

                2063    1241     842
 
SexAI A'CCwGG_T

Cuts at:      0    1592    4146 
   Size:       1592    2554
 
SfcI C'TryA_G

Cuts at:      0     870    1337    2077    2976    3253    3320    3334    4146 
   Size:        870     467     740     899     277      67      14     812

  Fragments arranged by size: 

                 899     870     812     740     467     277      67      14
            
 
SgrAI Cr'CCGG_yG

Cuts at:      0     793    4146 
   Size:        793    3353
 
SmaI CCC'GGG

Cuts at:      0    1464    4146 
   Size:       1464    2682
 
SmlI C'TyrA_G

Cuts at:      0     201     423    1690    2361    3665    4146 
   Size:        201     222    1267     671    1304     481

  Fragments arranged by size: 

                1304    1267     671     481     222     201
 
SphI G_CATG'C

Cuts at:      0    1260    1507    4146 
   Size:       1260     247    2639

  Fragments arranged by size: 

                2639    1260     247
 
SspI AAT'ATT

Cuts at:      0     545     571    4146 
   Size:        545      26    3575

  Fragments arranged by size: 

                3575     545      26
 
StuI AGG'CCT

Cuts at:      0    1527    1741    3023    4146 
   Size:       1527     214    1282    1123

  Fragments arranged by size: 

                1527    1282    1123     214
 
StyI C'CwwG_G

Cuts at:      0     600    1604    2537    2654    3173    3548    3581    3749 
   Size:        600    1004     933     117     519     375      33     168

Cuts at:   3749    3770    4146 
   Size:         21     376

  Fragments arranged by size: 

                1004     933     600     519     376     375     168     117
                  33      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      12    3477    4146 
   Size:         12    3465     669

  Fragments arranged by size: 

                3465     669      12
 
TatI w'GTAC_w

Cuts at:      0     531     840    1061    2081    3338    4146 
   Size:        531     309     221    1020    1257     808

  Fragments arranged by size: 

                1257    1020     808     531     309     221
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     336     347     368     379    1813    1845    2115    2147 
   Size:        336      11      21      11    1434      32     270      32

Cuts at:   2147    2297    2329    3375    3407    4146 
   Size:        150      32    1046      32     739

  Fragments arranged by size: 

                1434    1046     739     336     270     150      32      32
                  32      32      21      11      11
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1416    2544    3132    4146 
   Size:       1416    1128     588    1014

  Fragments arranged by size: 

                1416    1128    1014     588
 
XhoI C'TCGA_G

Cuts at:      0    1690    4146 
   Size:       1690    2456
 
XmaI C'CCGG_G

Cuts at:      0    1462    4146 
   Size:       1462    2684
 
XmnI GAAnn'nnTTC

Cuts at:      0    1360    2764    4146 
   Size:       1360    1404    1382

  Fragments arranged by size: 

                1404    1382    1360
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AgeI     AhdI     AleI     AloI
    AlwNI     ApaI     ApoI     AvaI     BaeI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    Hin4I   HincII
  HindIII     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NspI     PasI     PciI    PflMI     PfoI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI    PvuII     SacI
    SacII     SapI     ScaI    SexAI     SfcI    SgrAI     SmaI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AlfI    ApaLI     AscI
     AseI    AsiSI    AvrII    BamHI     BbeI    BfrBI    BmgBI     BmtI
    BsaBI    BsiWI     BsmI    BsmBI    BspEI   BssHII    BstBI  BstZ17I
    CspCI    EcoRV     FseI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     NarI     NheI     NotI     NruI     NsiI     PacI
     PmeI     PsrI     PvuI    RsrII     SalI    SanDI     SbfI     SfiI
     SfoI     SgfI    SnaBI     SpeI     SrfI     SwaI  Tth111I     XbaI
     ZraI
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