Restriction map for pF1KE0079
 (Linear) (Six-base) MAPSORT of: KIEE0079.seq  Check: 8514  from: 1  to: 1848



KIEE0079.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      63    1510    1602    1848 
   Size:         63    1447      92     246

  Fragments arranged by size: 

                1447     246      92      63
 
AccI GT'mk_AC

Cuts at:      0    1646    1848 
   Size:       1646     202
 
Acc65I G'GTAC_C

Cuts at:      0     350    1848 
   Size:        350    1498
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1002    1460    1848 
   Size:       1002     458     388

  Fragments arranged by size: 

                1002     458     388
 
AflIII A'CryG_T

Cuts at:      0     164     931    1848 
   Size:        164     767     917

  Fragments arranged by size: 

                 917     767     164
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1350    1848 
   Size:       1350     498
 
AlwNI CAG_nnn'CTG

Cuts at:      0     512    1080    1848 
   Size:        512     568     768

  Fragments arranged by size: 

                 768     568     512
 
ApaLI G'TGCA_C

Cuts at:      0     339    1848 
   Size:        339    1509
 
AscI GG'CGCG_CC

Cuts at:      0    1726    1848 
   Size:       1726     122
 
AvaI C'yCGr_G

Cuts at:      0    1477    1783    1812    1848 
   Size:       1477     306      29      36

  Fragments arranged by size: 

                1477     306      36      29
 
BanI G'GyrC_C

Cuts at:      0      20     350     412    1522    1848 
   Size:         20     330      62    1110     326

  Fragments arranged by size: 

                1110     330     326      62      20
 
BanII G_rGCy'C

Cuts at:      0    1220    1694    1848 
   Size:       1220     474     154

  Fragments arranged by size: 

                1220     474     154
 
BbeI G_GCGC'C

Cuts at:      0      24     416    1848 
   Size:         24     392    1432

  Fragments arranged by size: 

                1432     392      24
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     161     883    1016    1848 
   Size:        161     722     133     832

  Fragments arranged by size: 

                 832     722     161     133
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1803    1837    1848 
   Size:       1803      34      11

  Fragments arranged by size: 

                1803      34      11
 
BglI GCCn_nnn'nGGC

Cuts at:      0     295     467     676    1848 
   Size:        295     172     209    1172

  Fragments arranged by size: 

                1172     295     209     172
 
BlpI GC'TnA_GC

Cuts at:      0    1509    1848 
   Size:       1509     339
 
Bme1580I G_kGCm'C

Cuts at:      0     343     931    1848 
   Size:        343     588     917

  Fragments arranged by size: 

                 917     588     343
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1308    1332    1404    1848 
   Size:       1308      24      72     444

  Fragments arranged by size: 

                1308     444      72      24
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1827    1848 
   Size:       1827      21
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     378     889    1153    1587    1848 
   Size:        378     511     264     434     261

  Fragments arranged by size: 

                 511     434     378     264     261
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1463    1711    1848 
   Size:       1463     248     137

  Fragments arranged by size: 

                1463     248     137
 
BsaHI Gr'CG_yC

Cuts at:      0      21     413    1848 
   Size:         21     392    1435

  Fragments arranged by size: 

                1435     392      21
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1042    1072    1573    1603    1848 
   Size:       1042      30     501      30     245

  Fragments arranged by size: 

                1042     501     245      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      83     886    1729    1848 
   Size:         83     803     843     119

  Fragments arranged by size: 

                 843     803     119      83
 
BseYI C'CCAG_C

Cuts at:      0     346     416    1061    1312    1848 
   Size:        346      70     645     251     536

  Fragments arranged by size: 

                 645     536     346     251      70
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1194    1245    1320    1729    1843    1848 
   Size:       1194      51      75     409     114       5

  Fragments arranged by size: 

                1194     409     114      75      51       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     343    1186    1848 
   Size:        343     843     662

  Fragments arranged by size: 

                 843     662     343
 
Bsp1286I G_dGCh'C

Cuts at:      0     343     931    1186    1220    1694    1848 
   Size:        343     588     255      34     474     154

  Fragments arranged by size: 

                 588     474     343     255     154      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      63    1510    1602    1848 
   Size:         63    1447      92     246

  Fragments arranged by size: 

                1447     246      92      63
 
BsrDI GCAATG_nn'

Cuts at:      0    1287    1848 
   Size:       1287     561
 
BsrFI r'CCGG_y

Cuts at:      0     307    1818    1848 
   Size:        307    1511      30

  Fragments arranged by size: 

                1511     307      30
 
BssHII G'CGCG_C

Cuts at:      0       9      53     398    1726    1848 
   Size:          9      44     345    1328     122

  Fragments arranged by size: 

                1328     345     122      44       9
 
BssSI C'ACGA_G

Cuts at:      0    1378    1848 
   Size:       1378     470
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     426     458    1343    1848 
   Size:        426      32     885     505

  Fragments arranged by size: 

                 885     505     426      32
 
Bsu36I CC'TnA_GG

Cuts at:      0     789    1848 
   Size:        789    1059
 
BtgI C'CryG_G

Cuts at:      0     299     494    1694    1848 
   Size:        299     195    1200     154

  Fragments arranged by size: 

                1200     299     195     154
 
DraIII CAC_nnn'GTG

Cuts at:      0     395     449    1848 
   Size:        395      54    1399

  Fragments arranged by size: 

                1399     395      54
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     802    1245    1848 
   Size:        802     443     603

  Fragments arranged by size: 

                 802     603     443
 
EaeI y'GGCC_r

Cuts at:      0     296     762    1284    1548    1848 
   Size:        296     466     522     264     300

  Fragments arranged by size: 

                 522     466     300     296     264
 
EarI CTCTTCn'nnn_

Cuts at:      0     661    1848 
   Size:        661    1187
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     378     889    1002    1153    1460    1587    1848 
   Size:        378     511     113     151     307     127     261

  Fragments arranged by size: 

                 511     378     307     261     151     127     113
 
EcoO109I rG'GnC_Cy

Cuts at:      0     288    1055    1848 
   Size:        288     767     793

  Fragments arranged by size: 

                 793     767     288
 
HaeII r_GCGC'y

Cuts at:      0      24     333     416    1848 
   Size:         24     309      83    1432

  Fragments arranged by size: 

                1432     309      83      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      78     110     769     801    1042    1074    1848 
   Size:         78      32     659      32     241      32     774

  Fragments arranged by size: 

                 774     659     241      78      32      32      32
 
KasI G'GCGC_C

Cuts at:      0      20     412    1848 
   Size:         20     392    1436

  Fragments arranged by size: 

                1436     392      20
 
KpnI G_GTAC'C

Cuts at:      0     354    1848 
   Size:        354    1494
 
MscI TGG'CCA

Cuts at:      0    1286    1550    1848 
   Size:       1286     264     298

  Fragments arranged by size: 

                1286     298     264
 
MslI CAynn'nnrTG

Cuts at:      0    1639    1848 
   Size:       1639     209
 
MspA1I CmG'CkG

Cuts at:      0      30    1607    1848 
   Size:         30    1577     241

  Fragments arranged by size: 

                1577     241      30
 
NaeI GCC'GGC

Cuts at:      0     309    1848 
   Size:        309    1539
 
NarI GG'CG_CC

Cuts at:      0      21     413    1848 
   Size:         21     392    1435

  Fragments arranged by size: 

                1435     392      21
 
NcoI C'CATG_G

Cuts at:      0    1694    1848 
   Size:       1694     154
 
NgoMIV G'CCGG_C

Cuts at:      0     307    1848 
   Size:        307    1541
 
NspI r_CATG'y

Cuts at:      0     168     845     935    1848 
   Size:        168     677      90     913

  Fragments arranged by size: 

                 913     677     168      90
 
PciI A'CATG_T

Cuts at:      0     164     931    1848 
   Size:        164     767     917

  Fragments arranged by size: 

                 917     767     164
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1396    1848 
   Size:       1396     452
 
PfoI T'CCnGG_A

Cuts at:      0    1763    1848 
   Size:       1763      85
 
PspXI vC'TCGA_Gb

Cuts at:      0    1812    1848 
   Size:       1812      36
 
PstI C_TGCA'G

Cuts at:      0      74    1848 
   Size:         74    1774
 
SfcI C'TryA_G

Cuts at:      0      70     702    1848 
   Size:         70     632    1146

  Fragments arranged by size: 

                1146     632      70
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     295    1848 
   Size:        295    1553
 
SfoI GGC'GCC

Cuts at:      0      22     414    1848 
   Size:         22     392    1434

  Fragments arranged by size: 

                1434     392      22
 
SmlI C'TyrA_G

Cuts at:      0    1812    1848 
   Size:       1812      36
 
StuI AGG'CCT

Cuts at:      0    1810    1848 
   Size:       1810      38
 
StyI C'CwwG_G

Cuts at:      0     531    1694    1841    1848 
   Size:        531    1163     147       7

  Fragments arranged by size: 

                1163     531     147       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1012    1848 
   Size:       1012     836
 
TatI w'GTAC_w

Cuts at:      0     161     808    1848 
   Size:        161     647    1040

  Fragments arranged by size: 

                1040     647     161
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     358     390     433     465    1848 
   Size:        358      32      43      32    1383

  Fragments arranged by size: 

                1383     358      43      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1417    1480    1848 
   Size:       1417      63     368

  Fragments arranged by size: 

                1417     368      63
 
XhoI C'TCGA_G

Cuts at:      0    1812    1848 
   Size:       1812      36
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AlfI    AlwNI    ApaLI
     AscI     AvaI     BanI    BanII     BbeI     BbsI     BcgI     BglI
     BlpI Bme1580I     BmrI     BplI     BpmI     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI   Bsu36I     BtgI   DraIII     DrdI     EaeI
     EarI  Eco57MI EcoO109I    HaeII    Hin4I     KasI     KpnI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
    PflMI     PfoI    PspXI     PstI     SfcI     SfiI     SfoI     SmlI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI     AloI
     ApaI     ApoI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrGI    BstBI   BstEII    BstXI    BstYI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     MfeI     MluI     MmeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XmaI     XmnI     ZraI
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