Restriction map for
pF1KE0057
(Circular) (Six-base) MAPSORT of: pF1KE0057.seq Check: 2676 from: 1 to: 6650
pF1KE0057 6650 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1185 3296 1185
Size: 2111 4539
AccI GT'mk_AC
Cuts at: 3688 3688
Size: 6650
Acc65I G'GTAC_C
Cuts at: 3666 3666
Size: 6650
AclI AA'CG_TT
Cuts at: 6490 6490
Size: 6650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 201 506 1040 1149 1534 1567 1879 1939
Size: 65 305 534 109 385 33 312 60
Cuts at: 1939 2047 3265 3561 3628 4475 4907 5654 5922
Size: 108 1218 296 67 847 432 747 268
Cuts at: 5922 136
Size: 864
Fragments arranged by size:
1218 864 847 747 534 432 385 312
305 296 268 109 108 67 65 60
33
AfeI AGC'GCT
Cuts at: 1870 1936 1870
Size: 66 6584
AflIII A'CryG_T
Cuts at: 4002 5106 6083 4002
Size: 1104 977 4569
Fragments arranged by size:
4569 1104 977
AgeI A'CCGG_T
Cuts at: 5038 5038
Size: 6650
AleI CACnn'nnGTG
Cuts at: 674 674
Size: 6650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 763 3557 763
Size: 2794 3856
AlwNI CAG_nnn'CTG
Cuts at: 1390 1512 2159 2247 2320 2408 3288 3432 3955
Size: 122 647 88 73 88 880 144 523
Cuts at: 3955 5522 1390
Size: 1567 2518
Fragments arranged by size:
2518 1567 880 647 523 144 122 88
88 73
ApaI G_GGCC'C
Cuts at: 2714 2714
Size: 6650
ApaLI G'TGCA_C
Cuts at: 5420 5420
Size: 6650
ApoI r'AATT_y
Cuts at: 1393 1546 2080 3654 6003 6259 1393
Size: 153 534 1574 2349 256 1784
Fragments arranged by size:
2349 1784 1574 534 256 153
AseI AT'TA_AT
Cuts at: 20 20
Size: 6650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6650
AvaI C'yCGr_G
Cuts at: 1046 2086 3670 1046
Size: 1040 1584 4026
Fragments arranged by size:
4026 1584 1040
BamHI G'GATC_C
Cuts at: 589 2560 3675 589
Size: 1971 1115 3564
Fragments arranged by size:
3564 1971 1115
BanI G'GyrC_C
Cuts at: 1040 1197 1338 2643 2703 3019 3666 4080 4328
Size: 157 141 1305 60 316 647 414 248
Cuts at: 4328 4363 1040
Size: 35 3327
Fragments arranged by size:
3327 1305 647 414 316 248 157 141
60 35
BanII G_rGCy'C
Cuts at: 154 486 2093 2314 2714 2743 3494 3664 4694
Size: 332 1607 221 400 29 751 170 1030
Cuts at: 4694 154
Size: 2110
Fragments arranged by size:
2110 1607 1030 751 400 332 221 170
29
BbeI G_GCGC'C
Cuts at: 4084 4332 4084
Size: 248 6402
BbsI GAAGACnn'nnnn_
Cuts at: 419 653 1269 1356 419
Size: 234 616 87 5713
Fragments arranged by size:
5713 616 234 87
BbvCI CC'TCA_GC
Cuts at: 1637 1728 1637
Size: 91 6559
BciVI GTATCCnnnnn_n'
Cuts at: 1558 4548 5309 6650 1558
Size: 2990 761 1341 1558
Fragments arranged by size:
2990 1558 1341 761
BclI T'GATC_A
Cuts at: 732 4170 732
Size: 3438 3212
BglI GCCn_nnn'nGGC
Cuts at: 3559 3559
Size: 6650
BglII A'GATC_T
Cuts at: 959 1151 1911 4165 6202 959
Size: 192 760 2254 2037 1407
Fragments arranged by size:
2254 2037 1407 760 192
BlpI GC'TnA_GC
Cuts at: 926 1412 2414 3762 926
Size: 486 1002 1348 3814
Fragments arranged by size:
3814 1348 1002 486
Bme1580I G_kGCm'C
Cuts at: 1045 2139 2706 2714 3024 4275 4368 5424 1045
Size: 1094 567 8 310 1251 93 1056 2271
Fragments arranged by size:
2271 1251 1094 1056 567 310 93 8
BmgBI CAC'GTC
Cuts at: 1617 1617
Size: 6650
BmrI ACTGGGnnnn_n'
Cuts at: 668 783 1843 3862 3943 4278 6422 668
Size: 115 1060 2019 81 335 2144 896
Fragments arranged by size:
2144 2019 1060 896 335 115 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1551 2542 3260 5017 1551
Size: 991 718 1757 3184
Fragments arranged by size:
3184 1757 991 718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 895 1521 2055 3103 3155 3213 3454 593
Size: 302 626 534 1048 52 58 241 3789
Fragments arranged by size:
3789 1048 626 534 302 241 58 52
Bpu10I CC'TnA_GC
Cuts at: 756 947 1637 1728 1807 2172 3191 756
Size: 191 690 91 79 365 1019 4215
Fragments arranged by size:
4215 1019 690 365 191 91 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 324 2441 3062 3826 4705 5197 5495 145
Size: 179 2117 621 764 879 492 298 1300
Fragments arranged by size:
2117 1300 879 764 621 492 298 179
BsaI GGTCTCn'nnnn_
Cuts at: 2928 2964 6354 2928
Size: 36 3390 3224
Fragments arranged by size:
3390 3224 36
BsaAI yAC'GTr
Cuts at: 4003 4003
Size: 6650
BsaBI GATnn'nnATC
Cuts at: 6 1780 2559 4164 6201 6
Size: 1774 779 1605 2037 455
Fragments arranged by size:
2037 1774 1605 779 455
BsaHI Gr'CG_yC
Cuts at: 4081 4329 5031 6527 4081
Size: 248 702 1496 4204
Fragments arranged by size:
4204 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3839 4360 5038 5312 5459 5870 3839
Size: 521 678 274 147 411 4619
Fragments arranged by size:
4619 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1138 1168 2577 2607 1138
Size: 30 1409 30 5181
Fragments arranged by size:
5181 1409 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 302 846 1146 1879 1956 3053 302
Size: 544 300 733 77 1097 3899
Fragments arranged by size:
3899 1097 733 544 300 77
BseYI C'CCAG_C
Cuts at: 153 1118 1318 1900 2103 2201 2564 2970 3099
Size: 965 200 582 203 98 363 406 129
Cuts at: 3099 3153 3425 3487 4075 4833 5410 5906 153
Size: 54 272 62 588 758 577 496 897
Fragments arranged by size:
965 897 758 588 582 577 496 406
363 272 203 200 129 98 62 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1054
Size: 6650
BsiEI CG_ry'CG
Cuts at: 104 1844 2952 4192 4238 5446 104
Size: 1740 1108 1240 46 1208 1308
Fragments arranged by size:
1740 1308 1240 1208 1108 46
BsiHKAI G_wGCw'C
Cuts at: 805 2093 2173 2415 3664 4442 4632 5424 805
Size: 1288 80 242 1249 778 190 792 2031
Fragments arranged by size:
2031 1288 1249 792 778 242 190 80
Bsp1286I G_dGCh'C
Cuts at: 154 486 805 1045 2093 2139 2173 2314 2415
Size: 332 319 240 1048 46 34 141 101
Cuts at: 2415 2706 2714 2743 3024 3494 3664 4275 4368
Size: 291 8 29 281 470 170 611 93
Cuts at: 4368 4442 4632 4694 5424 154
Size: 74 190 62 730 1380
Fragments arranged by size:
1380 1048 730 611 470 332 319 291
281 240 190 170 141 101 93 74
62 46 34 29 8
BspEI T'CCGG_A
Cuts at: 5870 5870
Size: 6650
BspHI T'CATG_A
Cuts at: 5826 5826
Size: 6650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 299 1185 3296 3700 3914 4216 4597 299
Size: 886 2111 404 214 302 381 2352
Fragments arranged by size:
2352 2111 886 404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 129 2187 4942 4996 6480 6645 50
Size: 79 2058 2755 54 1484 165 55
Fragments arranged by size:
2755 2058 1484 165 79 55 54
BsrDI GCAATG_nn'
Cuts at: 4562 4562
Size: 6650
BsrFI r'CCGG_y
Cuts at: 638 4648 4829 5038 638
Size: 4010 181 209 2250
Fragments arranged by size:
4010 2250 209 181
BssSI C'ACGA_G
Cuts at: 4921 5279 4921
Size: 358 6292
BstAPI GCAn_nnn'nTGC
Cuts at: 1022 2793 3550 4027 1022
Size: 1771 757 477 3645
Fragments arranged by size:
3645 1771 757 477
BstBI TT'CG_AA
Cuts at: 5011 5011
Size: 6650
BstEII G'GTnAC_C
Cuts at: 1518 1518
Size: 6650
BstXI CCAn_nnnn'nTGG
Cuts at: 1890 2358 1890
Size: 468 6182
BstYI r'GATC_y
Cuts at: 1 555 589 696 893 959 1151 1775 1911
Size: 554 34 107 197 66 192 624 136
Cuts at: 1911 2560 3675 4142 4165 4500 4746 5758 5844
Size: 649 1115 467 23 335 246 1012 86
Cuts at: 5844 5856 6202 1
Size: 12 346 449
Fragments arranged by size:
1115 1012 649 624 554 467 449 346
335 246 197 192 136 107 86 66
34 23 12
BtgI C'CryG_G
Cuts at: 1844 2231 2855 1844
Size: 387 624 5639
Fragments arranged by size:
5639 624 387
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2647 3609 4600 4781 4944 2647
Size: 962 991 181 163 4353
Fragments arranged by size:
4353 991 962 181 163
BtsI GCAGTG_nn'
Cuts at: 348 750 990 1042 1189 2381 3551 3893 348
Size: 402 240 52 147 1192 1170 342 3105
Fragments arranged by size:
3105 1192 1170 402 342 240 147 52
DraI TTT'AAA
Cuts at: 3649 3649
Size: 6650
DrdI GACnn_nn'nnGTC
Cuts at: 2942 4356 5214 2942
Size: 1414 858 4378
Fragments arranged by size:
4378 1414 858
EaeI y'GGCC_r
Cuts at: 130 179 1595 1841 1943 2378 2949 4235 4409
Size: 49 1416 246 102 435 571 1286 174
Cuts at: 4409 4800 4827 6172 130
Size: 391 27 1345 608
Fragments arranged by size:
1416 1345 1286 608 571 435 391 246
174 102 49 27
EagI C'GGCC_G
Cuts at: 1841 2949 4235 1841
Size: 1108 1286 4256
Fragments arranged by size:
4256 1286 1108
EarI CTCTTCn'nnn_
Cuts at: 1164 1603 2840 3337 3410 4673 4883 1164
Size: 439 1237 497 73 1263 210 2931
Fragments arranged by size:
2931 1263 1237 497 439 210 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4858 5168 5314 6460 4858
Size: 310 146 1146 5048
Fragments arranged by size:
5048 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2091 3662 2091
Size: 1571 5079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 201 506 593 895 1040 1149 1521 1534
Size: 65 305 87 302 145 109 372 13
Cuts at: 1534 1567 1879 1939 2047 2055 3103 3155 3213
Size: 33 312 60 108 8 1048 52 58
Cuts at: 3213 3265 3454 3561 3628 4475 4907 5654 5922
Size: 52 189 107 67 847 432 747 268
Cuts at: 5922 136
Size: 864
Fragments arranged by size:
1048 864 847 747 432 372 312 305
302 268 189 145 109 108 107 87
67 65 60 58 52 52 33 13
8
EcoNI CCTnn'n_nnAGG
Cuts at: 306 1810 1894 3068 306
Size: 1504 84 1174 3888
Fragments arranged by size:
3888 1504 1174 84
EcoO109I rG'GnC_Cy
Cuts at: 261 1050 2244 2296 2317 2580 2671 2711 2944
Size: 789 1194 52 21 263 91 40 233
Cuts at: 2944 3012 3073 3537 3789 261
Size: 68 61 464 252 3122
Fragments arranged by size:
3122 1194 789 464 263 252 233 91
68 61 52 40 21
EcoRI G'AATT_C
Cuts at: 1393 3654 1393
Size: 2261 4389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1539 1597 2774 3223 3366 4669 1539
Size: 58 1177 449 143 1303 3520
Fragments arranged by size:
3520 1303 1177 449 143 58
FspI TGC'GCA
Cuts at: 4431 4431
Size: 6650
HaeII r_GCGC'y
Cuts at: 1872 1938 3361 4084 4332 5354 6333 1872
Size: 66 1423 723 248 1022 979 2189
Fragments arranged by size:
2189 1423 1022 979 723 248 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1138 1170 2530 2562 2817 2849 3319 3351 4492
Size: 32 1360 32 255 32 470 32 1141
Cuts at: 4492 4522 4524 4554 1138
Size: 30 2 30 3234
Fragments arranged by size:
3234 1360 1141 470 255 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3689 3689
Size: 6650
HindIII A'AGCT_T
Cuts at: 1475 1555 2985 3525 1475
Size: 80 1430 540 4600
Fragments arranged by size:
4600 1430 540 80
KasI G'GCGC_C
Cuts at: 4080 4328 4080
Size: 248 6402
KpnI G_GTAC'C
Cuts at: 3670 3670
Size: 6650
MluI A'CGCG_T
Cuts at: 6083 6083
Size: 6650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3390 5321 5505 3390
Size: 1931 184 4535
Fragments arranged by size:
4535 1931 184
MscI TGG'CCA
Cuts at: 181 1597 1945 2380 4411 181
Size: 1416 348 435 2031 2420
Fragments arranged by size:
2420 2031 1416 435 348
MslI CAynn'nnrTG
Cuts at: 674 2386 4639 4766 674
Size: 1712 2253 127 2558
Fragments arranged by size:
2558 2253 1712 127
MspA1I CmG'CkG
Cuts at: 136 834 1019 1411 3761 4075 4435 5448 5693
Size: 698 185 392 2350 314 360 1013 245
Cuts at: 5693 136
Size: 1093
Fragments arranged by size:
2350 1093 1013 698 392 360 314 245
185
NaeI GCC'GGC
Cuts at: 4831 4831
Size: 6650
NarI GG'CG_CC
Cuts at: 4081 4329 4081
Size: 248 6402
NcoI C'CATG_G
Cuts at: 2231 2855 2231
Size: 624 6026
NdeI CA'TA_TG
Cuts at: 1133 1133
Size: 6650
NgoMIV G'CCGG_C
Cuts at: 4829 4829
Size: 6650
NspI r_CATG'y
Cuts at: 396 2103 2864 3553 3703 4203 5110 396
Size: 1707 761 689 150 500 907 1936
Fragments arranged by size:
1936 1707 907 761 689 500 150
PasI CC'CwG_GG
Cuts at: 585 2367 2402 2743 2821 3107 585
Size: 1782 35 341 78 286 4128
Fragments arranged by size:
4128 1782 341 286 78 35
PciI A'CATG_T
Cuts at: 5106 5106
Size: 6650
PflMI CCAn_nnn'nTGG
Cuts at: 3049 3432 3049
Size: 383 6267
PfoI T'CCnGG_A
Cuts at: 3085 3258 3085
Size: 173 6477
PmeI GTTT'AAAC
Cuts at: 3649 3649
Size: 6650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5789 5821 5789
Size: 32 6618
PpuMI rG'GwC_Cy
Cuts at: 261 1050 2244 2296 2580 2671 2944 3012 3073
Size: 789 1194 52 284 91 273 68 61
Cuts at: 3073 261
Size: 3838
Fragments arranged by size:
3838 1194 789 284 273 91 68 61
52
PspOMI G'GGCC_C
Cuts at: 2710 2710
Size: 6650
PstI C_TGCA'G
Cuts at: 1196 1421 1687 2243 3278 3697 4382 1196
Size: 225 266 556 1035 419 685 3464
Fragments arranged by size:
3464 1035 685 556 419 266 225
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6650
PvuII CAG'CTG
Cuts at: 136 834 1019 1411 4075 4435 136
Size: 698 185 392 2664 360 2351
Fragments arranged by size:
2664 2351 698 392 360 185
RsrII CG'GwC_CG
Cuts at: 4845 4845
Size: 6650
SacI G_AGCT'C
Cuts at: 2093 3664 2093
Size: 1571 5079
SalI G'TCGA_C
Cuts at: 3687 3687
Size: 6650
SanDI GG'GwC_CC
Cuts at: 261 1050 2671 3012 261
Size: 789 1621 341 3899
Fragments arranged by size:
3899 1621 789 341
SapI GCTCTTCn'nnn_
Cuts at: 3337 4673 4883 3337
Size: 1336 210 5104
Fragments arranged by size:
5104 1336 210
SbfI CC_TGCA'GG
Cuts at: 3697 3697
Size: 6650
ScaI AGT'ACT
Cuts at: 6167 6167
Size: 6650
SexAI A'CCwGG_T
Cuts at: 2576 2576
Size: 6650
SfcI C'TryA_G
Cuts at: 33 1192 1417 1683 2019 2239 2331 2697 3274
Size: 1159 225 266 336 220 92 366 577
Cuts at: 3274 3567 3693 4378 5371 5562 33
Size: 293 126 685 993 191 1121
Fragments arranged by size:
1159 1121 993 685 577 366 336 293
266 225 220 191 126 92
SfoI GGC'GCC
Cuts at: 4082 4330 4082
Size: 248 6402
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6650
SmaI CCC'GGG
Cuts at: 3672 3672
Size: 6650
SmlI C'TyrA_G
Cuts at: 124 303 2456 3077 3805 4720 5212 5474 124
Size: 179 2153 621 728 915 492 262 1300
Fragments arranged by size:
2153 1300 915 728 621 492 262 179
SpeI A'CTAG_T
Cuts at: 67 940 67
Size: 873 5777
SphI G_CATG'C
Cuts at: 396 2864 3553 3703 4203 396
Size: 2468 689 150 500 2843
Fragments arranged by size:
2843 2468 689 500 150
SspI AAT'ATT
Cuts at: 242 242
Size: 6650
StuI AGG'CCT
Cuts at: 761 1426 1678 1693 2754 761
Size: 665 252 15 1061 4657
Fragments arranged by size:
4657 1061 665 252 15
StyI C'CwwG_G
Cuts at: 1427 1643 1768 2231 2596 2602 2639 2855 3784
Size: 216 125 463 365 6 37 216 929
Cuts at: 3784 4138 1427
Size: 354 3939
Fragments arranged by size:
3939 929 463 365 354 216 216 125
37 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 802 3083 4233 4366 4825 5034 802
Size: 2281 1150 133 459 209 2418
Fragments arranged by size:
2418 2281 1150 459 209 133
TatI w'GTAC_w
Cuts at: 1097 6165 1097
Size: 5068 1582
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1996 2028 6167 6199 1996
Size: 32 4139 32 2447
Fragments arranged by size:
4139 2447 32 32
Tth111I GACn'n_nGTC
Cuts at: 4447 4447
Size: 6650
XbaI T'CTAG_A
Cuts at: 3681 3681
Size: 6650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4145 4145
Size: 6650
XmaI C'CCGG_G
Cuts at: 3670 3670
Size: 6650
XmnI GAAnn'nnTTC
Cuts at: 499 499
Size: 6650
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BcgI
BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI