Restriction map for pF1KE0053
##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AclI        AflIII      AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvrII       BbrPI       
BbvCI       BclI        BlnI        BsiWI       BspDI       
BspHI       BspLU11I    BssSI       Bst1107I    BstAPI      
BstZ17I     Bsu36I      ClaI        CpoI        DrdI        
Eam1105I    Eco47III    Eco81I      FbaI        HpaI        
MfeI        MluI        MunI        NdeI        NheI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SbfI        SexAI       
SfiI        SgrAI       SnaBI       SpeI        Sse8387I    
StuI        SwaI        Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

Acc65I        524  GGTACC               G^GTACC
Alw44I       1440  GTGCAC               G^TGCAC
ApaLI        1440  GTGCAC               G^TGCAC
Asp718I       524  GGTACC               G^GTACC
BcgI         3365  CGAAACTCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        2700  AGATCT               A^GATCT
BlpI         2989  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2989  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2237  GATCCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstBI        1535  TTCGAA               TT^CGAA
BstEII       2892  GGTGACC              G^GT[ACGT]ACC
BstPI        2892  GGTGACC              G^GT[ACGT]ACC
CelII        2989  GCTGAGC              GC^T[ACGT]AGC
DraI         2436  TTTAAA               TTT^AAA
DraIII        697  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      2892  GGTGACC              G^GT[ACGT]ACC
FseI          348  GGCCGGCC             GGCCGG^CC
KpnI          524  GGTACC               GGTAC^C
KspI          587  CCGCGG               CCGC^GG
MamI         2237  GATCCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI         2646  CCATGG               C^CATGG
NotI          934  GCGGCCGC             GC^GGCCGC
NspV         1535  TTCGAA               TT^CGAA
PflMI         773  CCACCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        3247  GACAGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         587  CCGCGG               CCGC^GG
SalI         4065  GTCGAC               G^TCGAC
SapI         2225  GCTCTTC              GCTCTTC(1/4)
SfuI         1535  TTCGAA               TT^CGAA
SphI         1798  GCATGC               GCATG^C
Van91I        773  CCACCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AatII         910  GACGTC               GACGT^C
             4210  GACGTC              
AccI         3298  GTCTAC               GT^[AC][GT]AC
             4065  GTCGAC              
AccIII       2963  TCCGGA               T^CCGGA
             3579  TCCGGA              
AflII        1198  CTTAAG               C^TTAAG
             3723  CTTAAG              
AviII         440  TGCGCA               TGC^GCA
             2453  TGCGCA              
BamHI        2236  GGATCC               G^GATCC
             3889  GGATCC              
BciVI        1844  GTATCC               GTATCC(6/5)
             2201  GTATCC              
BfrI         1198  CTTAAG               C^TTAAG
             3723  CTTAAG              
BglI          111  GCCCGTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              142  GCCATCGCGGC         
BsaAI         547  TACGTG               [CT]AC^GT[GA]
             2975  TACGTG              
BsaI            6  GGTCTC               GGTCTC(1/5)
              270  GGTCTC              
BseAI        2963  TCCGGA               T^CCGGA
             3579  TCCGGA              
Bsp1407I     1461  TGTACA               T^GTACA
             3542  TGTACA              
BspEI        2963  TCCGGA               T^CCGGA
             3579  TCCGGA              
BspMI        1663  ACCTGC               ACCTGC(4/8)
             3573  GCAGGT              
BsrGI        1461  TGTACA               T^GTACA
             3542  TGTACA              
BstXI         890  CCAGCCTAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2205  CCAGTTTGATGG        
EcoNI         894  CCTAGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3082  CCTGAGTCAGG         
EcoRI        1346  GAATTC               G^AATTC
             3583  GAATTC              
EcoRV        3879  GATATC               GAT^ATC
             4420  GATATC              
EcoT22I      3121  ATGCAT               ATGCA^T
             4352  ATGCAT              
FspI          440  TGCGCA               TGC^GCA
             2453  TGCGCA              
MroI         2963  TCCGGA               T^CCGGA
             3579  TCCGGA              
NaeI          349  GCCGGC               GCC^GGC
              487  GCCGGC              
NgoMIV        349  GCCGGC               G^CCGGC
              487  GCCGGC              
NsiI         3121  ATGCAT               ATGCA^T
             4352  ATGCAT              
PpuMI        2525  AGGACCC              [GA]G^G[AT]CC[CT]
             3510  GGGTCCC             
PstI         2512  CTGCAG               CTGCA^G
             2995  CTGCAG              
ScaI         3589  AGTACT               AGT^ACT
             3941  AGTACT              
SmaI          301  CCCGGG               CCC^GGG
             2063  CCCGGG              
SspBI        1461  TGTACA               T^GTACA
             3542  TGTACA              
XmaCI         301  CCCGGG               C^CCGGG
             2063  CCCGGG              
XmaI          301  CCCGGG               C^CCGGG
             2063  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  128 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BsaWI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    BsrBI       BsrDI       
BsrFI       BsrI        BssHII      BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EagI        EarI        EclXI       
Eco52I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII       
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