Restriction map for pF1KE0053
 (Circular) (Six-base) MAPSORT of: pF1KE0053.seq  Check: 7709  from: 1  to: 7817



pF1KE0053  7817 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AatII G_ACGT'C

Cuts at:   1022    4322    1022 
   Size:       3300    4517
 
AccI GT'mk_AC

Cuts at:   3407    4174    4855    3407 
   Size:        767     681    6369

  Fragments arranged by size: 

                6369     767     681
 
Acc65I G'GTAC_C

Cuts at:    632    4833     632 
   Size:       4201    3616
 
AclI AA'CG_TT

Cuts at:   7657    7657 
   Size:       7817
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    405    1083    3242    3844    4022    4396    5642    6074    6821 
   Size:        678    2159     602     178     374    1246     432     747

Cuts at:   6821    7089     405 
   Size:        268    1133

  Fragments arranged by size: 

                2159    1246    1133     747     678     602     432     374
                 268     178
 
AflII C'TTAA_G

Cuts at:   1306    3831    1306 
   Size:       2525    5292
 
AflIII A'CryG_T

Cuts at:   5169    6273    7250    5169 
   Size:       1104     977    5736

  Fragments arranged by size: 

                5736    1104     977
 
AgeI A'CCGG_T

Cuts at:   6205    6205 
   Size:       7817
 
AleI CACnn'nnGTG

Cuts at:    562     655     922     562 
   Size:         93     267    7457

  Fragments arranged by size: 

                7457     267      93
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    448     562     940    1638     448 
   Size:        114     378     698    6627

  Fragments arranged by size: 

                6627     698     378     114
 
AlwNI CAG_nnn'CTG

Cuts at:    765    2017    2213    4315    5122    6689     765 
   Size:       1252     196    2102     807    1567    1893

  Fragments arranged by size: 

                2102    1893    1567    1252     807     196
 
ApaLI G'TGCA_C

Cuts at:   1548    6587    1548 
   Size:       5039    2778
 
ApoI r'AATT_y

Cuts at:   1326    1454    1631    1851    2266    2439    2693    3691    4029 
   Size:        128     177     220     415     173     254     998     338

Cuts at:   4029    4612    4648    4676    4821    7170    7426    1326 
   Size:        583      36      28     145    2349     256    1717

  Fragments arranged by size: 

                2349    1717     998     583     415     338     256     254
                 220     177     173     145     128      36      28
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       7817
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7817
 
AvaI C'yCGr_G

Cuts at:    409     463    2171    4837     409 
   Size:         54    1708    2666    3389

  Fragments arranged by size: 

                3389    2666    1708      54
 
BamHI G'GATC_C

Cuts at:   2344    3997    4842    2344 
   Size:       1653     845    5319

  Fragments arranged by size: 

                5319    1653     845
 
BanI G'GyrC_C

Cuts at:    129     387     502     556     632     770    1249    1529    4833 
   Size:        258     115      54      76     138     479     280    3304

Cuts at:   4833    5247    5495    5530     129 
   Size:        414     248      35    2416

  Fragments arranged by size: 

                3304    2416     479     414     280     258     248     138
                 115      76      54      35
 
BanII G_rGCy'C

Cuts at:    221     589    1742    2909    3071    3575    4248    4831    5861 
   Size:        368    1153    1167     162     504     673     583    1030

Cuts at:   5861     221 
   Size:       2177

  Fragments arranged by size: 

                2177    1167    1153    1030     673     583     504     368
                 162
 
BbeI G_GCGC'C

Cuts at:    133     391     506     774    5251    5499     133 
   Size:        258     115     268    4477     248    2451

  Fragments arranged by size: 

                4477    2451     268     258     248     115
 
BbsI GAAGACnn'nnnn_

Cuts at:   2032    2049    2082    3671    4414    2032 
   Size:         17      33    1589     743    5435

  Fragments arranged by size: 

                5435    1589     743      33      17
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3462    3496    3462 
   Size:         34    7783
 
BciVI GTATCCnnnnn_n'

Cuts at:   1963    2320    5715    6476    7817    1963 
   Size:        357    3395     761    1341    1963

  Fragments arranged by size: 

                3395    1963    1341     761     357
 
BclI T'GATC_A

Cuts at:   5337    5337 
   Size:       7817
 
BfrBI ATG'CAT

Cuts at:   3231    4462    3231 
   Size:       1231    6586
 
BglI GCCn_nnn'nGGC

Cuts at:    225     256     225 
   Size:         31    7786
 
BglII A'GATC_T

Cuts at:   2808    5332    7369    2808 
   Size:       2524    2037    3256

  Fragments arranged by size: 

                3256    2524    2037
 
BlpI GC'TnA_GC

Cuts at:   3098    4929    3098 
   Size:       1831    5986
 
Bme1580I G_kGCm'C

Cuts at:   1552    1627    3898    5442    5535    6591    1552 
   Size:         75    2271    1544      93    1056    2778

  Fragments arranged by size: 

                2778    2271    1544    1056      93      75
 
BmrI ACTGGGnnnn_n'

Cuts at:   2534    5029    5110    5445    7589    2534 
   Size:       2495      81     335    2144    2762

  Fragments arranged by size: 

                2762    2495    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    396    1086    6184     396 
   Size:        690    5098    2029

  Fragments arranged by size: 

                5098    2029     690
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    343     981    1070    1310    1631    2923     343 
   Size:        638      89     240     321    1292    5237

  Fragments arranged by size: 

                5237    1292     638     321     240      89
 
Bpu10I CC'TnA_GC

Cuts at:    532     665     532 
   Size:        133    7684
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    712     760    3901    4993    5872    6364    6662     712 
   Size:         48    3141    1092     879     492     298    1867

  Fragments arranged by size: 

                3141    1867    1092     879     492     298      48
 
BsaI GGTCTCn'nnnn_

Cuts at:    120     384    7521     120 
   Size:        264    7137     416

  Fragments arranged by size: 

                7137     416     264
 
BsaAI yAC'GTr

Cuts at:    657    3085    5170     657 
   Size:       2428    2085    3304

  Fragments arranged by size: 

                3304    2428    2085
 
BsaBI GATnn'nnATC

Cuts at:      6    2349    5331    7368       6 
   Size:       2343    2982    2037     455

  Fragments arranged by size: 

                2982    2343    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    130     388     503     771     815    1019    3242    4283    4319 
   Size:        258     115     268      44     204    2223    1041      36

Cuts at:   4319    5248    5496    6198    7694     130 
   Size:        929     248     702    1496     253

  Fragments arranged by size: 

                2223    1496    1041     929     702     268     258     253
                 248     204     115      44      36
 
BsaWI w'CCGG_w

Cuts at:   1766    3071    3687    5006    5527    6205    6479    6626    7037 
   Size:       1305     616    1319     521     678     274     147     411

Cuts at:   7037    1766 
   Size:       2546

  Fragments arranged by size: 

                2546    1319    1305     678     616     521     411     274
                 147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1443    1473    1553    1583    3916    3946    1443 
   Size:         30      80      30    2333      30    5314

  Fragments arranged by size: 

                5314    2333      80      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    979     984    1464    2051    3505     979 
   Size:          5     480     587    1454    5291

  Fragments arranged by size: 

                5291    1454     587     480       5
 
BseYI C'CCAG_C

Cuts at:    765     892    1139    1435    1902    2892    5242    6000    6577 
   Size:        127     247     296     467     990    2350     758     577

Cuts at:   6577    7073     765 
   Size:        496    1509

  Fragments arranged by size: 

                2350    1509     990     758     577     496     467     296
                 247     127
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    351     787    1758    3767     351 
   Size:        436     971    2009    4401

  Fragments arranged by size: 

                4401    2009     971     436
 
BsiEI CG_ry'CG

Cuts at:    104     204     232     458     498     596     978    1046    1157 
   Size:        100      28     226      40      98     382      68     111

Cuts at:   1157    2993    5359    5405    6613     104 
   Size:       1836    2366      46    1208    1308

  Fragments arranged by size: 

                2366    1836    1308    1208     382     226     111     100
                  98      68      46      40      28
 
BsiHKAI G_wGCw'C

Cuts at:    329     919    1552    1742    2909    2960    3071    4248    4831 
   Size:        590     633     190    1167      51     111    1177     583

Cuts at:   4831    5609    5799    6591     329 
   Size:        778     190     792    1555

  Fragments arranged by size: 

                1555    1177    1167     792     778     633     590     583
                 190     190     111      51
 
BsmI GAATG_Cn'

Cuts at:   1903    3826    4093    4450    1903 
   Size:       1923     267     357    5270

  Fragments arranged by size: 

                5270    1923     357     267
 
BsmBI CGTCTCn'nnnn_

Cuts at:    667    3233    3480     667 
   Size:       2566     247    5004

  Fragments arranged by size: 

                5004    2566     247
 
Bsp1286I G_dGCh'C

Cuts at:    221     329     589     919    1552    1627    1742    2909    2960 
   Size:        108     260     330     633      75     115    1167      51

Cuts at:   2960    3071    3575    3898    4248    4831    5442    5535    5609 
   Size:        111     504     323     350     583     611      93      74

Cuts at:   5609    5799    5861    6591     221 
   Size:        190      62     730    1447

  Fragments arranged by size: 

                1447    1167     730     633     611     583     504     350
                 330     323     260     190     115     111     108      93
                  75      74      62      51
 
BspEI T'CCGG_A

Cuts at:   3071    3687    7037    3071 
   Size:        616    3350    3851

  Fragments arranged by size: 

                3851    3350     616
 
BspHI T'CATG_A

Cuts at:   6993    6993 
   Size:       7817
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1780    3672    4867    5081    5383    5764    1780 
   Size:       1892    1195     214     302     381    3833

  Fragments arranged by size: 

                3833    1892    1195     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     290     872    1065    6109    6163    7647    7812      50 
   Size:        240     582     193    5044      54    1484     165      55

  Fragments arranged by size: 

                5044    1484     582     240     193     165      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   2357    2858    3383    5729    2357 
   Size:        501     525    2346    4445

  Fragments arranged by size: 

                4445    2346     525     501
 
BsrFI r'CCGG_y

Cuts at:    375     457     481     595     883    5815    5996    6205     375 
   Size:         82      24     114     288    4932     181     209    1987

  Fragments arranged by size: 

                4932    1987     288     209     181     114      82      24
            
 
BsrGI T'GTAC_A

Cuts at:   1569    3650    1569 
   Size:       2081    5736
 
BssHII G'CGCG_C

Cuts at:    901    1120    1122     901 
   Size:        219       2    7596

  Fragments arranged by size: 

                7596     219       2
 
BssSI C'ACGA_G

Cuts at:   6088    6446    6088 
   Size:        358    7459
 
BstAPI GCAn_nnn'nTGC

Cuts at:   5194    5194 
   Size:       7817
 
BstBI TT'CG_AA

Cuts at:   1644    6178    1644 
   Size:       4534    3283
 
BstEII G'GTnAC_C

Cuts at:   3000    3000 
   Size:       7817
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1005    2320    1005 
   Size:       1315    6502
 
BstYI r'GATC_y

Cuts at:      1    1112    2344    2808    3997    4311    4842    5309    5332 
   Size:       1111    1232     464    1189     314     531     467      23

Cuts at:   5332    5667    5913    6925    7011    7023    7369       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1232    1189    1111    1012     531     467     464     449
                 346     335     314     246      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     695     960    1082    1253    2754    2993    3623     107 
   Size:        588     265     122     171    1501     239     630    4301

  Fragments arranged by size: 

                4301    1501     630     588     265     239     171     122
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    237     705     891    5767    5948    6111     237 
   Size:        468     186    4876     181     163    1943

  Fragments arranged by size: 

                4876    1943     468     186     181     163
 
BtsI GCAGTG_nn'

Cuts at:    160    1164    1518    3388    4062    5060     160 
   Size:       1004     354    1870     674     998    2917

  Fragments arranged by size: 

                2917    1870    1004     998     674     354
 
DraI TTT'AAA

Cuts at:   2546    4816    2546 
   Size:       2270    5547
 
DraIII CAC_nnn'GTG

Cuts at:    810     810 
   Size:       7817
 
DrdI GACnn_nn'nnGTC

Cuts at:   5523    6381    5523 
   Size:        858    6959
 
EaeI y'GGCC_r

Cuts at:    201     455     593     644     716     752     957     963     975 
   Size:        254     138      51      72      36     205       6      12

Cuts at:    975    1043    1154    1296    2990    3798    3975    5402    5576 
   Size:         68     111     142    1694     808     177    1427     174

Cuts at:   5576    5967    5994    7339     201 
   Size:        391      27    1345     679

  Fragments arranged by size: 

                1694    1427    1345     808     679     391     254     205
                 177     174     142     138     111      72      68      51
                  36      27      12       6
 
EagI C'GGCC_G

Cuts at:    201     455     593     975    1043    1154    2990    5402     201 
   Size:        254     138     382      68     111    1836    2412    2616

  Fragments arranged by size: 

                2616    2412    1836     382     254     138     111      68
            
 
EarI CTCTTCn'nnn_

Cuts at:   1102    1364    2279    2340    2755    2874    4358    5840    6050 
   Size:        262     915      61     415     119    1484    1482     210

Cuts at:   6050    1102 
   Size:       2869

  Fragments arranged by size: 

                2869    1484    1482     915     415     262     210     119
                  61
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   6025    6335    6481    7627    6025 
   Size:        310     146    1146    6215

  Fragments arranged by size: 

                6215    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1740    2907    3069    4246    4829    1740 
   Size:       1167     162    1177     583    4728

  Fragments arranged by size: 

                4728    1177    1167     583     162
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    343     405     981    1070    1083    1310    1631    2923    3242 
   Size:         62     576      89      13     227     321    1292     319

Cuts at:   3242    3844    4022    4396    5642    6074    6821    7089     343 
   Size:        602     178     374    1246     432     747     268    1071

  Fragments arranged by size: 

                1292    1246    1071     747     602     576     432     374
                 321     319     268     227     178      89      62      13
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1006    3194    1006 
   Size:       2188    5629
 
EcoO109I rG'GnC_Cy

Cuts at:   1011    2634    3619    4956    1011 
   Size:       1623     985    1337    3872

  Fragments arranged by size: 

                3872    1623    1337     985
 
EcoRI G'AATT_C

Cuts at:   1454    3691    4821    1454 
   Size:       2237    1130    4450

  Fragments arranged by size: 

                4450    2237    1130
 
EcoRV GAT'ATC

Cuts at:   3989    4530    3989 
   Size:        541    7276
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1568    5836    1568 
   Size:       4268    3549
 
FseI GG_CCGG'CC

Cuts at:    461     461 
   Size:       7817
 
FspI TGC'GCA

Cuts at:    550    2563    5598     550 
   Size:       2013    3035    2769

  Fragments arranged by size: 

                3035    2769    2013
 
FspAI rTGC'GCAy

Cuts at:   2563    2563 
   Size:       7817
 
HaeII r_GCGC'y

Cuts at:    133     160     391     506     774     878    1071    1090    5251 
   Size:         27     231     115     268     104     193      19    4161

Cuts at:   5251    5499    6521    7500     133 
   Size:        248    1022     979     450

  Fragments arranged by size: 

                4161    1022     979     450     268     248     231     193
                 115     104      27      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    553     585    1222    1254    1591    1623    2014    2046    2908 
   Size:         32     637      32     337      32     391      32     862

Cuts at:   2908    2940    3916    3948    5659    5689    5691    5721     553 
   Size:         32     976      32    1711      30       2      30    2649

  Fragments arranged by size: 

                2649    1711     976     862     637     391     337      32
                  32      32      32      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1824    3043    4175    4856    1824 
   Size:       1219    1132     681    4785

  Fragments arranged by size: 

                4785    1219    1132     681
 
HindIII A'AGCT_T

Cuts at:   1683    3297    4510    1683 
   Size:       1614    1213    4990

  Fragments arranged by size: 

                4990    1614    1213
 
KasI G'GCGC_C

Cuts at:    129     387     502     770    5247    5495     129 
   Size:        258     115     268    4477     248    2451

  Fragments arranged by size: 

                4477    2451     268     258     248     115
 
KpnI G_GTAC'C

Cuts at:    636    4837     636 
   Size:       4201    3616
 
MluI A'CGCG_T

Cuts at:   7250    7250 
   Size:       7817
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    212     728    1221    1253    3546    3739    6488    6672     212 
   Size:        516     493      32    2293     193    2749     184    1357

  Fragments arranged by size: 

                2749    2293    1357     516     493     193     184      32
            
 
MscI TGG'CCA

Cuts at:    754    1298    3800    3977    5578     754 
   Size:        544    2502     177    1601    2993

  Fragments arranged by size: 

                2993    2502    1601     544     177
 
MslI CAynn'nnrTG

Cuts at:    562     655     922    2188    2260    4643    5806    5933     562 
   Size:         93     267    1266      72    2383    1163     127    2446

  Fragments arranged by size: 

                2446    2383    1266    1163     267     127      93      72
            
 
MspA1I CmG'CkG

Cuts at:    296     309     629     686     697    1048    1139    1435    4928 
   Size:         13     320      57      11     351      91     296    3493

Cuts at:   4928    5242    5602    6615    6860     296 
   Size:        314     360    1013     245    1253

  Fragments arranged by size: 

                3493    1253    1013     360     351     320     314     296
                 245      91      57      13      11
 
NaeI GCC'GGC

Cuts at:    459     597    5998     459 
   Size:        138    5401    2278

  Fragments arranged by size: 

                5401    2278     138
 
NarI GG'CG_CC

Cuts at:    130     388     503     771    5248    5496     130 
   Size:        258     115     268    4477     248    2451

  Fragments arranged by size: 

                4477    2451     268     258     248     115
 
NcoI C'CATG_G

Cuts at:    107    2754     107 
   Size:       2647    5170
 
NgoMIV G'CCGG_C

Cuts at:    457     595    5996     457 
   Size:        138    5401    2278

  Fragments arranged by size: 

                5401    2278     138
 
NotI GC'GGCC_GC

Cuts at:   1043    1043 
   Size:       7817
 
NsiI A_TGCA'T

Cuts at:   3233    4464    3233 
   Size:       1231    6586
 
NspI r_CATG'y

Cuts at:   1910    3468    4771    4870    5370    6277    1910 
   Size:       1558    1303      99     500     907    3450

  Fragments arranged by size: 

                3450    1558    1303     907     500      99
 
PasI CC'CwG_GG

Cuts at:    522     522 
   Size:       7817
 
PciI A'CATG_T

Cuts at:   6273    6273 
   Size:       7817
 
PflMI CCAn_nnn'nTGG

Cuts at:    887     887 
   Size:       7817
 
PfoI T'CCnGG_A

Cuts at:    118    3554    3731     118 
   Size:       3436     177    4204

  Fragments arranged by size: 

                4204    3436     177
 
PmeI GTTT'AAAC

Cuts at:   4816    4816 
   Size:       7817
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    913     945    6956    6988     913 
   Size:         32    6011      32    1742

  Fragments arranged by size: 

                6011    1742      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2634    3619    2634 
   Size:        985    6832
 
PshAI GACnn'nnGTC

Cuts at:   3359    3359 
   Size:       7817
 
PstI C_TGCA'G

Cuts at:   2624    3107    4864    5549    2624 
   Size:        483    1757     685    4892

  Fragments arranged by size: 

                4892    1757     685     483
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       7817
 
PvuII CAG'CTG

Cuts at:    296     309    1139    1435    5242    5602     296 
   Size:         13     830     296    3807     360    2511

  Fragments arranged by size: 

                3807    2511     830     360     296      13
 
RsrII CG'GwC_CG

Cuts at:   6012    6012 
   Size:       7817
 
SacI G_AGCT'C

Cuts at:   1742    2909    3071    4248    4831    1742 
   Size:       1167     162    1177     583    4728

  Fragments arranged by size: 

                4728    1177    1167     583     162
 
SacII CC_GC'GG

Cuts at:    698     698 
   Size:       7817
 
SalI G'TCGA_C

Cuts at:   4173    4854    4173 
   Size:        681    7136
 
SanDI GG'GwC_CC

Cuts at:   3619    3619 
   Size:       7817
 
SapI GCTCTTCn'nnn_

Cuts at:   2340    5840    6050    2340 
   Size:       3500     210    4107

  Fragments arranged by size: 

                4107    3500     210
 
SbfI CC_TGCA'GG

Cuts at:   4864    4864 
   Size:       7817
 
ScaI AGT'ACT

Cuts at:   3699    4051    7334    3699 
   Size:        352    3283    4182

  Fragments arranged by size: 

                4182    3283     352
 
SfcI C'TryA_G

Cuts at:     33     315    1664    1779    2620    3103    4860    5545    6538 
   Size:        282    1349     115     841     483    1757     685     993

Cuts at:   6538    6729      33 
   Size:        191    1121

  Fragments arranged by size: 

                1757    1349    1121     993     841     685     483     282
                 191     115
 
SfoI GGC'GCC

Cuts at:    131     389     504     772    5249    5497     131 
   Size:        258     115     268    4477     248    2451

  Fragments arranged by size: 

                4477    2451     268     258     248     115
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7817
 
SmaI CCC'GGG

Cuts at:    411    2173    4839     411 
   Size:       1762    2666    3389

  Fragments arranged by size: 

                3389    2666    1762
 
SmlI C'TyrA_G

Cuts at:    727     775    1306    3831    3880    4972    5887    6379    6641 
   Size:         48     531    2525      49    1092     915     492     262

Cuts at:   6641     727 
   Size:       1903

  Fragments arranged by size: 

                2525    1903    1092     915     531     492     262      49
                  48
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       7817
 
SphI G_CATG'C

Cuts at:   1910    4870    5370    1910 
   Size:       2960     500    4357

  Fragments arranged by size: 

                4357    2960     500
 
SspI AAT'ATT

Cuts at:   2506    2721    2793    3765    4699    2506 
   Size:        215      72     972     934    5624

  Fragments arranged by size: 

                5624     972     934     215      72
 
StyI C'CwwG_G

Cuts at:    107    2048    2754    4178    4523    4951    5305     107 
   Size:       1941     706    1424     345     428     354    2619

  Fragments arranged by size: 

                2619    1941    1424     706     428     354     345
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2272    3223    5400    5533    5992    6201    2272 
   Size:        951    2177     133     459     209    3888

  Fragments arranged by size: 

                3888    2177     951     459     209     133
 
TatI w'GTAC_w

Cuts at:   1569    2589    3650    3697    4049    7332    1569 
   Size:       1020    1061      47     352    3283    2054

  Fragments arranged by size: 

                3283    2054    1061    1020     352      47
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1443    1475    2394    2426    7334    7366    1443 
   Size:         32     919      32    4908      32    1894

  Fragments arranged by size: 

                4908    1894     919      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   5614    5614 
   Size:       7817
 
XbaI T'CTAG_A

Cuts at:   4848    4848 
   Size:       7817
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    762    2320    2761    3444    5312     762 
   Size:       1558     441     683    1868    3267

  Fragments arranged by size: 

                3267    1868    1558     683     441
 
XmaI C'CCGG_G

Cuts at:    409    2171    4837     409 
   Size:       1762    2666    3389

  Fragments arranged by size: 

                3389    2666    1762
 
ZraI GAC'GTC

Cuts at:   1020    4320    1020 
   Size:       3300    4517
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NotI     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI    PshAI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AhdI     AloI     ApaI     AscI    AvrII     BaeI
    BbvCI    BmgBI     BmtI    BsiWI  BstZ17I   Bsu36I     ClaI    CspCI
     HpaI     MfeI     NdeI     NheI     NruI     PacI     PmlI     PsiI
   PspOMI    PspXI     PsrI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     StuI     SwaI     XhoI     XmnI
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