Restriction map for pF1KE0053
 (Linear) (Six-base) MAPSORT of: KIEE0053.seq  Check: 5844  from: 1  to: 4704



KIEE0053.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AatII G_ACGT'C

Cuts at:      0     914    4214    4704 
   Size:        914    3300     490

  Fragments arranged by size: 

                3300     914     490
 
AccI GT'mk_AC

Cuts at:      0    3299    4066    4704 
   Size:       3299     767     638

  Fragments arranged by size: 

                3299     767     638
 
Acc65I G'GTAC_C

Cuts at:      0     524    4704 
   Size:        524    4180
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     975    3134    3736    3914    4288    4704 
   Size:        297     678    2159     602     178     374     416

  Fragments arranged by size: 

                2159     678     602     416     374     297     178
 
AflII C'TTAA_G

Cuts at:      0    1198    3723    4704 
   Size:       1198    2525     981

  Fragments arranged by size: 

                2525    1198     981
 
AleI CACnn'nnGTG

Cuts at:      0     454     547     814    4704 
   Size:        454      93     267    3890

  Fragments arranged by size: 

                3890     454     267      93
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     340     454     832    1530    4704 
   Size:        340     114     378     698    3174

  Fragments arranged by size: 

                3174     698     378     340     114
 
AlwNI CAG_nnn'CTG

Cuts at:      0     657    1909    2105    4207    4704 
   Size:        657    1252     196    2102     497

  Fragments arranged by size: 

                2102    1252     657     497     196
 
ApaLI G'TGCA_C

Cuts at:      0    1440    4704 
   Size:       1440    3264
 
ApoI r'AATT_y

Cuts at:      0    1218    1346    1523    1743    2158    2331    2585    3583 
   Size:       1218     128     177     220     415     173     254     998

Cuts at:   3583    3921    4504    4540    4568    4704 
   Size:        338     583      36      28     136

  Fragments arranged by size: 

                1218     998     583     415     338     254     220     177
                 173     136     128      36      28
 
AvaI C'yCGr_G

Cuts at:      0     301     355    2063    4704 
   Size:        301      54    1708    2641

  Fragments arranged by size: 

                2641    1708     301      54
 
BamHI G'GATC_C

Cuts at:      0    2236    3889    4704 
   Size:       2236    1653     815

  Fragments arranged by size: 

                2236    1653     815
 
BanI G'GyrC_C

Cuts at:      0      21     279     394     448     524     662    1141    1421 
   Size:         21     258     115      54      76     138     479     280

Cuts at:   1421    4704 
   Size:       3283

  Fragments arranged by size: 

                3283     479     280     258     138     115      76      54
                  21
 
BanII G_rGCy'C

Cuts at:      0     113     481    1634    2801    2963    3467    4140    4704 
   Size:        113     368    1153    1167     162     504     673     564

  Fragments arranged by size: 

                1167    1153     673     564     504     368     162     113
            
 
BbeI G_GCGC'C

Cuts at:      0      25     283     398     666    4704 
   Size:         25     258     115     268    4038

  Fragments arranged by size: 

                4038     268     258     115      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1924    1941    1974    3563    4306    4704 
   Size:       1924      17      33    1589     743     398

  Fragments arranged by size: 

                1924    1589     743     398      33      17
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3354    3388    4704 
   Size:       3354      34    1316

  Fragments arranged by size: 

                3354    1316      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1855    2212    4704 
   Size:       1855     357    2492

  Fragments arranged by size: 

                2492    1855     357
 
BfrBI ATG'CAT

Cuts at:      0    3123    4354    4704 
   Size:       3123    1231     350

  Fragments arranged by size: 

                3123    1231     350
 
BglI GCCn_nnn'nGGC

Cuts at:      0     117     148    4704 
   Size:        117      31    4556

  Fragments arranged by size: 

                4556     117      31
 
BglII A'GATC_T

Cuts at:      0    2700    4704 
   Size:       2700    2004
 
BlpI GC'TnA_GC

Cuts at:      0    2990    4704 
   Size:       2990    1714
 
Bme1580I G_kGCm'C

Cuts at:      0    1444    1519    3790    4704 
   Size:       1444      75    2271     914

  Fragments arranged by size: 

                2271    1444     914      75
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2426    4704 
   Size:       2426    2278
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     288     978    4704 
   Size:        288     690    3726

  Fragments arranged by size: 

                3726     690     288
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     873     962    1202    1523    2815    4704 
   Size:        235     638      89     240     321    1292    1889

  Fragments arranged by size: 

                1889    1292     638     321     240     235      89
 
Bpu10I CC'TnA_GC

Cuts at:      0     424     557    4704 
   Size:        424     133    4147

  Fragments arranged by size: 

                4147     424     133
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     652    3793    4704 
   Size:        604      48    3141     911

  Fragments arranged by size: 

                3141     911     604      48
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      12     276    4704 
   Size:         12     264    4428

  Fragments arranged by size: 

                4428     264      12
 
BsaAI yAC'GTr

Cuts at:      0     549    2977    4704 
   Size:        549    2428    1727

  Fragments arranged by size: 

                2428    1727     549
 
BsaBI GATnn'nnATC

Cuts at:      0    2241    4704 
   Size:       2241    2463
 
BsaHI Gr'CG_yC

Cuts at:      0      22     280     395     663     707     911    3134    4175 
   Size:         22     258     115     268      44     204    2223    1041

Cuts at:   4175    4211    4704 
   Size:         36     493

  Fragments arranged by size: 

                2223    1041     493     268     258     204     115      44
                  36      22
 
BsaWI w'CCGG_w

Cuts at:      0    1658    2963    3579    4704 
   Size:       1658    1305     616    1125

  Fragments arranged by size: 

                1658    1305    1125     616
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1335    1365    1445    1475    3808    3838    4704 
   Size:       1335      30      80      30    2333      30     866

  Fragments arranged by size: 

                2333    1335     866      80      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     871     876    1356    1943    3397    4704 
   Size:        871       5     480     587    1454    1307

  Fragments arranged by size: 

                1454    1307     871     587     480       5
 
BseYI C'CCAG_C

Cuts at:      0     657     784    1031    1327    1794    2784    4704 
   Size:        657     127     247     296     467     990    1920

  Fragments arranged by size: 

                1920     990     657     467     296     247     127
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     243     679    1650    3659    4704 
   Size:        243     436     971    2009    1045

  Fragments arranged by size: 

                2009    1045     971     436     243
 
BsiEI CG_ry'CG

Cuts at:      0      96     124     350     390     488     870     938    1049 
   Size:         96      28     226      40      98     382      68     111

Cuts at:   1049    2885    4704 
   Size:       1836    1819

  Fragments arranged by size: 

                1836    1819     382     226     111      98      96      68
                  40      28
 
BsiHKAI G_wGCw'C

Cuts at:      0     221     811    1444    1634    2801    2852    2963    4140 
   Size:        221     590     633     190    1167      51     111    1177

Cuts at:   4140    4704 
   Size:        564

  Fragments arranged by size: 

                1177    1167     633     590     564     221     190     111
                  51
 
BsmI GAATG_Cn'

Cuts at:      0    1795    3718    3985    4342    4704 
   Size:       1795    1923     267     357     362

  Fragments arranged by size: 

                1923    1795     362     357     267
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     559    3125    3372    4704 
   Size:        559    2566     247    1332

  Fragments arranged by size: 

                2566    1332     559     247
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     221     481     811    1444    1519    1634    2801 
   Size:        113     108     260     330     633      75     115    1167

Cuts at:   2801    2852    2963    3467    3790    4140    4704 
   Size:         51     111     504     323     350     564

  Fragments arranged by size: 

                1167     633     564     504     350     330     323     260
                 115     113     111     108      75      51
 
BspEI T'CCGG_A

Cuts at:      0    2963    3579    4704 
   Size:       2963     616    1125

  Fragments arranged by size: 

                2963    1125     616
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1672    3564    4704 
   Size:       1672    1892    1140

  Fragments arranged by size: 

                1892    1672    1140
 
BsrBI CCG'CTC

Cuts at:      0     182     764     957    4704 
   Size:        182     582     193    3747

  Fragments arranged by size: 

                3747     582     193     182
 
BsrDI GCAATG_nn'

Cuts at:      0    2249    2750    3275    4704 
   Size:       2249     501     525    1429

  Fragments arranged by size: 

                2249    1429     525     501
 
BsrFI r'CCGG_y

Cuts at:      0     267     349     373     487     775    4704 
   Size:        267      82      24     114     288    3929

  Fragments arranged by size: 

                3929     288     267     114      82      24
 
BsrGI T'GTAC_A

Cuts at:      0    1461    3542    4704 
   Size:       1461    2081    1162

  Fragments arranged by size: 

                2081    1461    1162
 
BssHII G'CGCG_C

Cuts at:      0     793    1012    1014    4704 
   Size:        793     219       2    3690

  Fragments arranged by size: 

                3690     793     219       2
 
BstBI TT'CG_AA

Cuts at:      0    1536    4704 
   Size:       1536    3168
 
BstEII G'GTnAC_C

Cuts at:      0    2892    4704 
   Size:       2892    1812
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     897    2212    4704 
   Size:        897    1315    2492

  Fragments arranged by size: 

                2492    1315     897
 
BstYI r'GATC_y

Cuts at:      0    1004    2236    2700    3889    4203    4704 
   Size:       1004    1232     464    1189     314     501

  Fragments arranged by size: 

                1232    1189    1004     501     464     314
 
BtgI C'CryG_G

Cuts at:      0     587     852     974    1145    2646    2885    3515    4704 
   Size:        587     265     122     171    1501     239     630    1189

  Fragments arranged by size: 

                1501    1189     630     587     265     239     171     122
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     129     597     783    4704 
   Size:        129     468     186    3921

  Fragments arranged by size: 

                3921     468     186     129
 
BtsI GCAGTG_nn'

Cuts at:      0      52    1056    1410    3280    3954    4704 
   Size:         52    1004     354    1870     674     750

  Fragments arranged by size: 

                1870    1004     750     674     354      52
 
DraI TTT'AAA

Cuts at:      0    2438    4704 
   Size:       2438    2266
 
DraIII CAC_nnn'GTG

Cuts at:      0     702    4704 
   Size:        702    4002
 
EaeI y'GGCC_r

Cuts at:      0      93     347     485     536     608     644     849     855 
   Size:         93     254     138      51      72      36     205       6

Cuts at:    855     867     935    1046    1188    2882    3690    3867    4704 
   Size:         12      68     111     142    1694     808     177     837

  Fragments arranged by size: 

                1694     837     808     254     205     177     142     138
                 111      93      72      68      51      36      12       6
            
 
EagI C'GGCC_G

Cuts at:      0      93     347     485     867     935    1046    2882    4704 
   Size:         93     254     138     382      68     111    1836    1822

  Fragments arranged by size: 

                1836    1822     382     254     138     111      93      68
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     994    1256    2171    2232    2647    2766    4250    4704 
   Size:        994     262     915      61     415     119    1484     454

  Fragments arranged by size: 

                1484     994     915     454     415     262     119      61
            
 
EcoICRI GAG'CTC

Cuts at:      0    1632    2799    2961    4138    4704 
   Size:       1632    1167     162    1177     566

  Fragments arranged by size: 

                1632    1177    1167     566     162
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     297     873     962     975    1202    1523    2815 
   Size:        235      62     576      89      13     227     321    1292

Cuts at:   2815    3134    3736    3914    4288    4704 
   Size:        319     602     178     374     416

  Fragments arranged by size: 

                1292     602     576     416     374     321     319     235
                 227     178      89      62      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     898    3086    4704 
   Size:        898    2188    1618

  Fragments arranged by size: 

                2188    1618     898
 
EcoO109I rG'GnC_Cy

Cuts at:      0     903    2526    3511    4704 
   Size:        903    1623     985    1193

  Fragments arranged by size: 

                1623    1193     985     903
 
EcoRI G'AATT_C

Cuts at:      0    1346    3583    4704 
   Size:       1346    2237    1121

  Fragments arranged by size: 

                2237    1346    1121
 
EcoRV GAT'ATC

Cuts at:      0    3881    4422    4704 
   Size:       3881     541     282

  Fragments arranged by size: 

                3881     541     282
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1460    4704 
   Size:       1460    3244
 
FseI GG_CCGG'CC

Cuts at:      0     353    4704 
   Size:        353    4351
 
FspI TGC'GCA

Cuts at:      0     442    2455    4704 
   Size:        442    2013    2249

  Fragments arranged by size: 

                2249    2013     442
 
FspAI rTGC'GCAy

Cuts at:      0    2455    4704 
   Size:       2455    2249
 
HaeII r_GCGC'y

Cuts at:      0      25      52     283     398     666     770     963     982 
   Size:         25      27     231     115     268     104     193      19

Cuts at:    982    4704 
   Size:       3722

  Fragments arranged by size: 

                3722     268     231     193     115     104      27      25
                  19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477    1114    1146    1483    1515    1906    1938 
   Size:        445      32     637      32     337      32     391      32

Cuts at:   1938    2800    2832    3808    3840    4704 
   Size:        862      32     976      32     864

  Fragments arranged by size: 

                 976     864     862     637     445     391     337      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1716    2935    4067    4704 
   Size:       1716    1219    1132     637

  Fragments arranged by size: 

                1716    1219    1132     637
 
HindIII A'AGCT_T

Cuts at:      0    1575    3189    4402    4704 
   Size:       1575    1614    1213     302

  Fragments arranged by size: 

                1614    1575    1213     302
 
KasI G'GCGC_C

Cuts at:      0      21     279     394     662    4704 
   Size:         21     258     115     268    4042

  Fragments arranged by size: 

                4042     268     258     115      21
 
KpnI G_GTAC'C

Cuts at:      0     528    4704 
   Size:        528    4176
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     620    1113    1145    3438    3631    4704 
   Size:        104     516     493      32    2293     193    1073

  Fragments arranged by size: 

                2293    1073     516     493     193     104      32
 
MscI TGG'CCA

Cuts at:      0     646    1190    3692    3869    4704 
   Size:        646     544    2502     177     835

  Fragments arranged by size: 

                2502     835     646     544     177
 
MslI CAynn'nnrTG

Cuts at:      0     454     547     814    2080    2152    4535    4704 
   Size:        454      93     267    1266      72    2383     169

  Fragments arranged by size: 

                2383    1266     454     267     169      93      72
 
MspA1I CmG'CkG

Cuts at:      0     188     201     521     578     589     940    1031    1327 
   Size:        188      13     320      57      11     351      91     296

Cuts at:   1327    4704 
   Size:       3377

  Fragments arranged by size: 

                3377     351     320     296     188      91      57      13
                  11
 
NaeI GCC'GGC

Cuts at:      0     351     489    4704 
   Size:        351     138    4215

  Fragments arranged by size: 

                4215     351     138
 
NarI GG'CG_CC

Cuts at:      0      22     280     395     663    4704 
   Size:         22     258     115     268    4041

  Fragments arranged by size: 

                4041     268     258     115      22
 
NcoI C'CATG_G

Cuts at:      0    2646    4704 
   Size:       2646    2058
 
NgoMIV G'CCGG_C

Cuts at:      0     349     487    4704 
   Size:        349     138    4217

  Fragments arranged by size: 

                4217     349     138
 
NotI GC'GGCC_GC

Cuts at:      0     935    4704 
   Size:        935    3769
 
NsiI A_TGCA'T

Cuts at:      0    3125    4356    4704 
   Size:       3125    1231     348

  Fragments arranged by size: 

                3125    1231     348
 
NspI r_CATG'y

Cuts at:      0    1802    3360    4663    4704 
   Size:       1802    1558    1303      41

  Fragments arranged by size: 

                1802    1558    1303      41
 
PasI CC'CwG_GG

Cuts at:      0     414    4704 
   Size:        414    4290
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     779    4704 
   Size:        779    3925
 
PfoI T'CCnGG_A

Cuts at:      0      10    3446    3623    4704 
   Size:         10    3436     177    1081

  Fragments arranged by size: 

                3436    1081     177      10
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     805     837    4704 
   Size:        805      32    3867

  Fragments arranged by size: 

                3867     805      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2526    3511    4704 
   Size:       2526     985    1193

  Fragments arranged by size: 

                2526    1193     985
 
PshAI GACnn'nnGTC

Cuts at:      0    3251    4704 
   Size:       3251    1453
 
PstI C_TGCA'G

Cuts at:      0    2516    2999    4704 
   Size:       2516     483    1705

  Fragments arranged by size: 

                2516    1705     483
 
PvuII CAG'CTG

Cuts at:      0     188     201    1031    1327    4704 
   Size:        188      13     830     296    3377

  Fragments arranged by size: 

                3377     830     296     188      13
 
SacI G_AGCT'C

Cuts at:      0    1634    2801    2963    4140    4704 
   Size:       1634    1167     162    1177     564

  Fragments arranged by size: 

                1634    1177    1167     564     162
 
SacII CC_GC'GG

Cuts at:      0     590    4704 
   Size:        590    4114
 
SalI G'TCGA_C

Cuts at:      0    4065    4704 
   Size:       4065     639
 
SanDI GG'GwC_CC

Cuts at:      0    3511    4704 
   Size:       3511    1193
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2232    4704 
   Size:       2232    2472
 
ScaI AGT'ACT

Cuts at:      0    3591    3943    4704 
   Size:       3591     352     761

  Fragments arranged by size: 

                3591     761     352
 
SfcI C'TryA_G

Cuts at:      0     207    1556    1671    2512    2995    4704 
   Size:        207    1349     115     841     483    1709

  Fragments arranged by size: 

                1709    1349     841     483     207     115
 
SfoI GGC'GCC

Cuts at:      0      23     281     396     664    4704 
   Size:         23     258     115     268    4040

  Fragments arranged by size: 

                4040     268     258     115      23
 
SmaI CCC'GGG

Cuts at:      0     303    2065    4704 
   Size:        303    1762    2639

  Fragments arranged by size: 

                2639    1762     303
 
SmlI C'TyrA_G

Cuts at:      0     619     667    1198    3723    3772    4704 
   Size:        619      48     531    2525      49     932

  Fragments arranged by size: 

                2525     932     619     531      49      48
 
SphI G_CATG'C

Cuts at:      0    1802    4704 
   Size:       1802    2902
 
SspI AAT'ATT

Cuts at:      0    2398    2613    2685    3657    4591    4704 
   Size:       2398     215      72     972     934     113

  Fragments arranged by size: 

                2398     972     934     215     113      72
 
StyI C'CwwG_G

Cuts at:      0    1940    2646    4070    4415    4704 
   Size:       1940     706    1424     345     289

  Fragments arranged by size: 

                1940    1424     706     345     289
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2164    3115    4704 
   Size:       2164     951    1589

  Fragments arranged by size: 

                2164    1589     951
 
TatI w'GTAC_w

Cuts at:      0    1461    2481    3542    3589    3941    4704 
   Size:       1461    1020    1061      47     352     763

  Fragments arranged by size: 

                1461    1061    1020     763     352      47
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1335    1367    2286    2318    4704 
   Size:       1335      32     919      32    2386

  Fragments arranged by size: 

                2386    1335     919      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     654    2212    2653    3336    4704 
   Size:        654    1558     441     683    1368

  Fragments arranged by size: 

                1558    1368     683     654     441
 
XmaI C'CCGG_G

Cuts at:      0     301    2063    4704 
   Size:        301    1762    2641

  Fragments arranged by size: 

                2641    1762     301
 
ZraI GAC'GTC

Cuts at:      0     912    4212    4704 
   Size:        912    3300     492

  Fragments arranged by size: 

                3300     912     492
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI    AflII     AleI     AlfI    AlwNI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI   DraIII     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NsiI     NspI
     PasI    PflMI     PfoI     PpiI    PpuMI    PshAI     PstI    PvuII
     SacI    SacII     SalI    SanDI     SapI     ScaI     SfcI     SfoI
     SmaI     SmlI     SphI     SspI     StyI    TaqII     TatI     TstI
     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI   AflIII     AgeI     AhdI     AloI     ApaI
     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI     BclI    BmgBI
     BmtI    BsiWI    BspHI    BssSI   BstAPI  BstZ17I   Bsu36I     ClaI
    CspCI     DrdI     EciI     HpaI     MfeI     MluI     NdeI     NheI
     NruI     PacI     PciI     PmeI     PmlI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SbfI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     StuI     SwaI  Tth111I     XbaI     XhoI
     XmnI
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