(Circular) (Six-base) MAPSORT of: pF1KE0050.seq Check: 5436 from: 1 to: 4520 pF1KE0050 4520 bp With 182 enzymes: SgfI * April 24, 2009 13:09 .. AccI GT'mk_AC Cuts at: 600 1481 1558 600 Size: 881 77 3562 Fragments arranged by size: 3562 881 77 Acc65I G'GTAC_C Cuts at: 1536 1536 Size: 4520 AclI AA'CG_TT Cuts at: 4360 4360 Size: 4520 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 437 1443 2345 2777 3524 3792 437 Size: 1006 902 432 747 268 1165 Fragments arranged by size: 1165 1006 902 747 432 268 AfeI AGC'GCT Cuts at: 821 821 Size: 4520 AflIII A'CryG_T Cuts at: 1015 1872 2976 3953 1015 Size: 857 1104 977 1582 Fragments arranged by size: 1582 1104 977 857 AgeI A'CCGG_T Cuts at: 2908 2908 Size: 4520 AhdI GACnn_n'nnGTC Cuts at: 788 1376 1460 788 Size: 588 84 3848 Fragments arranged by size: 3848 588 84 AleI CACnn'nnGTG Cuts at: 746 746 Size: 4520 AlwNI CAG_nnn'CTG Cuts at: 1825 3392 1825 Size: 1567 2953 ApaLI G'TGCA_C Cuts at: 3290 3290 Size: 4520 ApoI r'AATT_y Cuts at: 199 485 1524 3873 4129 199 Size: 286 1039 2349 256 590 Fragments arranged by size: 2349 1039 590 286 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4520 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4520 AvaI C'yCGr_G Cuts at: 1540 1540 Size: 4520 BamHI G'GATC_C Cuts at: 1545 1545 Size: 4520 BanI G'GyrC_C Cuts at: 1536 1950 2198 2233 1536 Size: 414 248 35 3823 Fragments arranged by size: 3823 414 248 35 BanII G_rGCy'C Cuts at: 1534 2564 1534 Size: 1030 3490 BbeI G_GCGC'C Cuts at: 1954 2202 1954 Size: 248 4272 BbsI GAAGACnn'nnnn_ Cuts at: 121 121 Size: 4520 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1356 1390 1356 Size: 34 4486 BciVI GTATCCnnnnn_n' Cuts at: 2418 3179 4520 2418 Size: 761 1341 2418 Fragments arranged by size: 2418 1341 761 BclI T'GATC_A Cuts at: 327 2040 327 Size: 1713 2807 BglII A'GATC_T Cuts at: 2035 4072 2035 Size: 2037 2483 BlpI GC'TnA_GC Cuts at: 1632 1632 Size: 4520 Bme1580I G_kGCm'C Cuts at: 2145 2238 3294 2145 Size: 93 1056 3371 Fragments arranged by size: 3371 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1732 1813 2148 4292 1732 Size: 81 335 2144 1960 Fragments arranged by size: 2144 1960 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2887 2887 Size: 4520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 851 935 1019 1103 1271 1355 1439 851 Size: 84 84 84 168 84 84 3932 Fragments arranged by size: 3932 168 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1696 2575 3067 3365 1696 Size: 879 492 298 2851 Fragments arranged by size: 2851 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4224 4224 Size: 4520 BsaAI yAC'GTr Cuts at: 1873 1873 Size: 4520 BsaBI GATnn'nnATC Cuts at: 6 2034 4071 6 Size: 2028 2037 455 Fragments arranged by size: 2037 2028 455 BsaHI Gr'CG_yC Cuts at: 1951 2199 2901 4397 1951 Size: 248 702 1496 2074 Fragments arranged by size: 2074 1496 702 248 BsaWI w'CCGG_w Cuts at: 1709 2230 2908 3182 3329 3740 1709 Size: 521 678 274 147 411 2489 Fragments arranged by size: 2489 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1945 2703 3280 3776 1945 Size: 758 577 496 2689 Fragments arranged by size: 2689 758 577 496 BsiEI CG_ry'CG Cuts at: 104 2062 2108 3316 104 Size: 1958 46 1208 1308 Fragments arranged by size: 1958 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1534 2312 2502 3294 1534 Size: 778 190 792 2760 Fragments arranged by size: 2760 792 778 190 Bsp1286I G_dGCh'C Cuts at: 1534 2145 2238 2312 2502 2564 3294 1534 Size: 611 93 74 190 62 730 2760 Fragments arranged by size: 2760 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3740 3740 Size: 4520 BspHI T'CATG_A Cuts at: 3696 3696 Size: 4520 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1570 1784 2086 2467 1570 Size: 214 302 381 3623 Fragments arranged by size: 3623 381 302 214 BsrBI CCG'CTC Cuts at: 50 2812 2866 4350 4515 50 Size: 2762 54 1484 165 55 Fragments arranged by size: 2762 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 166 2432 166 Size: 2266 2254 BsrFI r'CCGG_y Cuts at: 2518 2699 2908 2518 Size: 181 209 4130 Fragments arranged by size: 4130 209 181 BssSI C'ACGA_G Cuts at: 2791 3149 2791 Size: 358 4162 BstAPI GCAn_nnn'nTGC Cuts at: 1897 1897 Size: 4520 BstBI TT'CG_AA Cuts at: 2881 2881 Size: 4520 BstYI r'GATC_y Cuts at: 1 1545 2012 2035 2370 2616 3628 3714 3726 Size: 1544 467 23 335 246 1012 86 12 Cuts at: 3726 4072 1 Size: 346 449 Fragments arranged by size: 1544 1012 467 449 346 335 246 86 23 12 BstZ17I GTA'TAC Cuts at: 601 1482 601 Size: 881 3639 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1373 2470 2651 2814 1373 Size: 1097 181 163 3079 Fragments arranged by size: 3079 1097 181 163 BtsI GCAGTG_nn' Cuts at: 1763 1763 Size: 4520 DraI TTT'AAA Cuts at: 1519 1519 Size: 4520 DrdI GACnn_nn'nnGTC Cuts at: 123 182 2226 3084 123 Size: 59 2044 858 1559 Fragments arranged by size: 2044 1559 858 59 EaeI y'GGCC_r Cuts at: 270 2105 2279 2670 2697 4042 270 Size: 1835 174 391 27 1345 748 Fragments arranged by size: 1835 1345 748 391 174 27 EagI C'GGCC_G Cuts at: 2105 2105 Size: 4520 EarI CTCTTCn'nnn_ Cuts at: 659 811 1100 2543 2753 659 Size: 152 289 1443 210 2426 Fragments arranged by size: 2426 1443 289 210 152 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2728 3038 3184 4330 2728 Size: 310 146 1146 2918 Fragments arranged by size: 2918 1146 310 146 EcoICRI GAG'CTC Cuts at: 1532 1532 Size: 4520 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 437 851 935 1019 1103 1271 1355 1439 1443 Size: 414 84 84 84 168 84 84 4 Cuts at: 1443 2345 2777 3524 3792 437 Size: 902 432 747 268 1165 Fragments arranged by size: 1165 902 747 432 414 268 168 84 84 84 84 84 4 EcoO109I rG'GnC_Cy Cuts at: 1659 1659 Size: 4520 EcoRI G'AATT_C Cuts at: 1524 1524 Size: 4520 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 382 2539 382 Size: 2157 2363 FspI TGC'GCA Cuts at: 2301 2301 Size: 4520 HaeII r_GCGC'y Cuts at: 823 1954 2202 3224 4203 823 Size: 1131 248 1022 979 1140 Fragments arranged by size: 1140 1131 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 774 806 1362 1394 1446 1478 2362 2392 2394 Size: 32 556 32 52 32 884 30 2 Cuts at: 2394 2424 774 Size: 30 2870 Fragments arranged by size: 2870 884 556 52 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 128 1559 128 Size: 1431 3089 HindIII A'AGCT_T Cuts at: 375 375 Size: 4520 KasI G'GCGC_C Cuts at: 1950 2198 1950 Size: 248 4272 KpnI G_GTAC'C Cuts at: 1540 1540 Size: 4520 MluI A'CGCG_T Cuts at: 3953 3953 Size: 4520 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3191 3375 3191 Size: 184 4336 MscI TGG'CCA Cuts at: 272 2281 272 Size: 2009 2511 MslI CAynn'nnrTG Cuts at: 561 746 930 1266 2509 2636 561 Size: 185 184 336 1243 127 2445 Fragments arranged by size: 2445 1243 336 185 184 127 MspA1I CmG'CkG Cuts at: 1631 1945 2305 3318 3563 1631 Size: 314 360 1013 245 2588 Fragments arranged by size: 2588 1013 360 314 245 NaeI GCC'GGC Cuts at: 2701 2701 Size: 4520 NarI GG'CG_CC Cuts at: 1951 2199 1951 Size: 248 4272 NgoMIV G'CCGG_C Cuts at: 2699 2699 Size: 4520 NspI r_CATG'y Cuts at: 340 1019 1573 2073 2980 340 Size: 679 554 500 907 1880 Fragments arranged by size: 1880 907 679 554 500 PciI A'CATG_T Cuts at: 1015 2976 1015 Size: 1961 2559 PmeI GTTT'AAAC Cuts at: 1519 1519 Size: 4520 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3659 3691 3659 Size: 32 4488 PsiI TTA'TAA Cuts at: 1396 1396 Size: 4520 PstI C_TGCA'G Cuts at: 1567 2252 1567 Size: 685 3835 PvuI CG_AT'CG Cuts at: 104 104 Size: 4520 PvuII CAG'CTG Cuts at: 1945 2305 1945 Size: 360 4160 RsrII CG'GwC_CG Cuts at: 2715 2715 Size: 4520 SacI G_AGCT'C Cuts at: 1534 1534 Size: 4520 SalI G'TCGA_C Cuts at: 1557 1557 Size: 4520 SapI GCTCTTCn'nnn_ Cuts at: 2543 2753 2543 Size: 210 4310 SbfI CC_TGCA'GG Cuts at: 1567 1567 Size: 4520 ScaI AGT'ACT Cuts at: 4037 4037 Size: 4520 SfcI C'TryA_G Cuts at: 33 1563 2248 3241 3432 33 Size: 1530 685 993 191 1121 Fragments arranged by size: 1530 1121 993 685 191 SfoI GGC'GCC Cuts at: 1952 2200 1952 Size: 248 4272 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4520 SmaI CCC'GGG Cuts at: 1542 1542 Size: 4520 SmlI C'TyrA_G Cuts at: 1675 2590 3082 3344 1675 Size: 915 492 262 2851 Fragments arranged by size: 2851 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4520 SphI G_CATG'C Cuts at: 340 1573 2073 340 Size: 1233 500 2787 Fragments arranged by size: 2787 1233 500 StyI C'CwwG_G Cuts at: 1654 2008 1654 Size: 354 4166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 297 1366 2103 2236 2695 2904 297 Size: 1069 737 133 459 209 1913 Fragments arranged by size: 1913 1069 737 459 209 133 TatI w'GTAC_w Cuts at: 313 570 4035 313 Size: 257 3465 798 Fragments arranged by size: 3465 798 257 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1149 1181 4037 4069 1149 Size: 32 2856 32 1600 Fragments arranged by size: 2856 1600 32 32 Tth111I GACn'n_nGTC Cuts at: 120 2317 120 Size: 2197 2323 XbaI T'CTAG_A Cuts at: 1551 1551 Size: 4520 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2015 2015 Size: 4520 XmaI C'CCGG_G Cuts at: 1540 1540 Size: 4520 XmnI GAAnn'nnTTC Cuts at: 531 531 Size: 4520 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AlfI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI