Restriction map for pF1KB8144
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BciVI       BclI        BfrI        
BlnI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FokI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NcoI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        TaqI        TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AluI          384  AGCT                 AG^CT
Asp700I       628  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI           39  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          135  CTCGGG               C^[CT]CG[GA]G
AvaII         525  GGACC                G^G[AT]CC
BbsI          350  GAAGAC               GAAGAC(2/6)
BcgI          537  CGAGAAGAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          105  CCGGG                CC^[GC]GG
BfaI          578  CTAG                 C^TAG
BglI          104  GCCGGGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         630  AGATCT               A^GATCT
BpmI          146  CTGGAG               CTGGAG(16/14)
BpuAI         350  GAAGAC               GAAGAC(2/6)
BsaI          587  GAGACC               GGTCTC(1/5)
BsgI          545  GTGCAG               GTGCAG(16/14)
BsmFI         151  GGGAC                GGGAC(10/14)
BsoBI         135  CTCGGG               C^[CT]CG[GA]G
BspMI          22  ACCTGC               ACCTGC(4/8)
BsrDI          94  CATTGC               GCAATG(2/0)
BstAPI         26  GCAGCGTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         522  CCAGG                CC^[AT]GG
DraI          361  TTTAAA               TTT^AAA
DrdI           39  GACCACGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          102  TGGCCG               [CT]^GGCC[GA]
EarI          540  GAAGAG               CTCTTC(1/4)
EcoRII        522  CCAGG                ^CC[AT]GG
EcoT14I       637  CCAAGG               C^C[AT][AT]GG
Ksp632I       540  GAAGAG               CTCTTC(1/4)
MaeI          578  CTAG                 C^TAG
MaeIII        693  GTCAC                ^GT[ACGT]AC
MslI          551  CACTGACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          522  CCAGG                CC^[AT]GG
NciI          105  CCGGG                CC^[GC]GG
NdeI          744  CATATG               CA^TATG
PflFI          39  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
StyI          637  CCAAGG               C^C[AT][AT]GG
SwaI          360  ATTTAAAT             ATTT^AAAT
Tsp45I        693  GTCAC                ^GT[GC]AC
Tth111I        39  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI           90  CCATCATTGCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          628  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII         114  CGCG                 CG^CG
              183  CGCG                
AcsI          260  AAATTC               [GA]^AATT[CT]
              658  GAATTT              
AlwI           81  GATCC                GGATC(4/5)
              598  GATCC               
ApoI          260  AAATTC               [GA]^AATT[CT]
              658  GAATTT              
BsaJI         105  CCGGGG               C^C[ACGT][ACGT]GG
              637  CCAAGG              
BsiYI          50  CCTCCATGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              730  CCAGAGAAGGG         
BslI           50  CCTCCATGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              730  CCAGAGAAGGG         
BsmAI         587  GAGAC                GTCTC(1/5)
              328  GTCTC               
BssKI         105  CCGGG                ^CC[ACGT]GG
              522  CCAGG               
BstUI         114  CGCG                 CG^CG
              183  CGCG                
BstYI          80  AGATCC               [GA]^GATC[CT]
              630  AGATCT              
HapII         105  CCGG                 C^CGG
              310  CCGG                
HpaII         105  CCGG                 C^CGG
              310  CCGG                
HphI          454  GGTGA                GGTGA(8/7)
              703  TCACC               
MaeII          43  ACGT                 A^CGT
              556  ACGT                
MflI           80  AGATCC               [GA]^GATC[CT]
              630  AGATCT              
MseI          362  TTAA                 T^TAA
              782  TTAA                
MspI          105  CCGG                 C^CGG
              310  CCGG                
MvnI          114  CGCG                 CG^CG
              183  CGCG                
PleI          750  GACTC                GAGTC(4/5)
              391  GAGTC               
PstI           24  CTGCAG               CTGCA^G
              413  CTGCAG              
ScrFI         105  CCGGG                CC^[ACGT]GG
              522  CCAGG               
SfcI           24  CTGCAG               C^T[GA][CT]AG
              413  CTGCAG              
TfiI          418  GAATC                G^A[AT]TC
              787  GAATC               
Tru9I         362  TTAA                 T^TAA
              782  TTAA                
XhoII          80  AGATCC               [GA]^GATC[CT]
              630  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        BbvI        BsrI        Cac8I       CfoI        
Cfr13I      DdeI        DpnII       FauI        Fnu4HI      
HaeIII      HhaI        HinP1I      HinfI       ItaI        
MboI        MboII       MnlI        MspA1I      MwoI        
NdeII       NlaIII      Sau3AI      Sau96I      TseI        
Tsp509I     TspRI       
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