Restriction map for pF1KB5605
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AocI        ApoI        AscI        AseI        
AsnI        Asp700I     AvrII       BalI        BamHI       
BbsI        BciVI       BclI        BfrI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        DraI        DrdI        EarI        Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        HindIII     HpaI        Ksp632I     MamI        
MboII       MfeI        MluNI       MroI        MscI        
MseI        MslI        MunI        NcoI        NdeI        
NotI        NsiI        NspV        PacI        PaeR7I      
PflMI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        SacI        SalI        SapI        
ScaI        SfiI        SfuI        SmlI        SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
SwaI        TfiI        Tru9I       Tsp509I     Van91I      
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I       1378  GGTACC               G^GTACC
AflIII        761  ACGCGT               A^C[GA][CT]GT
AgeI          962  ACCGGT               A^CCGGT
AhdI         1230  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        669  GTGCAC               G^TGCAC
Aor51HI      1013  AGCGCT               AGC^GCT
ApaLI         669  GTGCAC               G^TGCAC
Asp718I      1378  GGTACC               G^GTACC
AspEI        1230  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          852  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1616  TGCGCA               TGC^GCA
BbrPI        1693  CACGTG               CAC^GTG
BbvCI         427  GCTGAGG              CCTCAGC(-5/-2)
BglII         149  AGATCT               A^GATCT
BpmI          991  CTGGAG               CTGGAG(16/14)
BsaAI        1693  CACGTG               [CT]AC^GT[GA]
BsaI          931  GAGACC               GGTCTC(1/5)
BsaWI         962  ACCGGT               [AT]^CCGG[AT]
BsmBI         564  CGTCTC               CGTCTC(1/5)
BsmFI         728  GGGAC                GGGAC(10/14)
BsmI         1539  GCATTC               GAATGC(1/-1)
BsrDI         767  GCAATG               GCAATG(2/0)
BssHII         23  GCGCGC               G^CGCGC
BstYI         149  AGATCT               [GA]^GATC[CT]
CpoI         1699  CGGACCG              CG^G[AT]CCG
DdeI          428  CTGAG                C^T[ACGT]AG
DraIII       1028  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1230  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1013  AGCGCT               AGC^GCT
FseI           36  GGCCGGCC             GGCCGG^CC
FspI         1616  TGCGCA               TGC^GCA
HincII       1021  GTCAAC               GT[CT]^[GA]AC
HindII       1021  GTCAAC               GT[CT]^[GA]AC
HphI          572  TCACC                GGTGA(8/7)
KpnI         1378  GGTACC               GGTAC^C
MflI          149  AGATCT               [GA]^GATC[CT]
MluI          761  ACGCGT               A^CGCGT
NheI         1267  GCTAGC               G^CTAGC
NlaIII        924  CATG                 CATG^
NruI         1568  TCGCGA               TCG^CGA
NspI          923  GCATGT               [GA]CATG^[CT]
PflFI         852  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         962  ACCGGT               A^CCGGT
PmaCI        1693  CACGTG               CAC^GTG
PmlI         1693  CACGTG               CAC^GTG
PpuMI           8  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         271  CAGCTG               CAG^CTG
RsrII        1699  CGGACCG              CG^G[AT]CCG
SexAI        1067  ACCTGGT              A^CC[AT]GGT
SmaI          117  CCCGGG               CCC^GGG
Tsp45I       1439  GTGAC                ^GT[GC]AC
Tth111I       852  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1637  CCAGGGCCAAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         149  AGATCT               [GA]^GATC[CT]
XmaCI         117  CCCGGG               C^CCGGG
XmaI          117  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwI          774  GATCC                GGATC(4/5)
              260  GGATC               
AlwNI         613  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1135  CAGCCCCTG           
AvaI          117  CCCGGG               C^[CT]CG[GA]G
              304  CTCGGG              
BfaI         1151  CTAG                 C^TAG
             1268  CTAG                
BsgI          837  CTGCAC               GTGCAG(16/14)
             1314  CTGCAC              
BsiEI         950  CGGCCG               CG[GA][CT]^CG
             1523  CGGCCG              
BsoBI         117  CCCGGG               C^[CT]CG[GA]G
              304  CTCGGG              
BstAPI         80  GCAGCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1689  GCAGCACGTGC         
EagI          950  CGGCCG               C^GGCCG
             1523  CGGCCG              
EclXI         950  CGGCCG               C^GGCCG
             1523  CGGCCG              
Eco52I        950  CGGCCG               C^GGCCG
             1523  CGGCCG              
EcoT14I        18  CCTTGG               C^C[AT][AT]GG
             1673  CCAAGG              
MaeI         1151  CTAG                 C^TAG
             1268  CTAG                
MaeII         974  ACGT                 A^CGT
             1694  ACGT                
MaeIII        331  GTTAC                ^GT[ACGT]AC
             1439  GTGAC               
SbfI          521  CCTGCAGG             CCTGCA^GG
              816  CCTGCAGG            
SgrAI         247  CGCCGGCG             C[GA]^CCGG[CT]G
              961  CACCGGTG            
Sse8387I      521  CCTGCAGG             CCTGCA^GG
              816  CCTGCAGG            
StyI           18  CCTTGG               C^C[AT][AT]GG
             1673  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
ApaI        AspHI       AvaII       BanI        BanII       
BbeI        BbvI        BcgI        BcnI        BglI        
BmyI        BsaHI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
ItaI        KasI        KspI        MboI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIV       PleI        PstI        RsaI        SacII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        TaqI        TseI        TspRI       TthHB8I     
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