Restriction map for pF1KB9977
 (Circular) (Six-base) MAPSORT of: pF1KB9977.seq  Check: 581  from: 1  to: 4784



pF1KB9977  4784 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    172     172 
   Size:       4784
 
AccI GT'mk_AC

Cuts at:    221    1822     221 
   Size:       1601    3183
 
Acc65I G'GTAC_C

Cuts at:   1800    1800 
   Size:       4784
 
AclI AA'CG_TT

Cuts at:    995    4624     995 
   Size:       3629    1155
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    539     611    1085    2609    3041    3788    4056     539 
   Size:         72     474    1524     432     747     268    1267

  Fragments arranged by size: 

                1524    1267     747     474     432     268      72
 
AflIII A'CryG_T

Cuts at:   2136    3240    4217    2136 
   Size:       1104     977    2703

  Fragments arranged by size: 

                2703    1104     977
 
AgeI A'CCGG_T

Cuts at:   3172    3172 
   Size:       4784
 
AleI CACnn'nnGTG

Cuts at:   1186    1186 
   Size:       4784
 
AlwNI CAG_nnn'CTG

Cuts at:   2089    3656    2089 
   Size:       1567    3217
 
ApaLI G'TGCA_C

Cuts at:   3554    3554 
   Size:       4784
 
ApoI r'AATT_y

Cuts at:    671    1010    1415    1433    1788    4137    4393     671 
   Size:        339     405      18     355    2349     256    1062

  Fragments arranged by size: 

                2349    1062     405     355     339     256      18
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4784
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4784
 
AvaI C'yCGr_G

Cuts at:   1804    1804 
   Size:       4784
 
BamHI G'GATC_C

Cuts at:   1809    1809 
   Size:       4784
 
BanI G'GyrC_C

Cuts at:    156    1800    2214    2462    2497     156 
   Size:       1644     414     248      35    2443

  Fragments arranged by size: 

                2443    1644     414     248      35
 
BanII G_rGCy'C

Cuts at:   1798    2828    1798 
   Size:       1030    3754
 
BbeI G_GCGC'C

Cuts at:    160    2218    2466     160 
   Size:       2058     248    2478

  Fragments arranged by size: 

                2478    2058     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1476    1500    1476 
   Size:         24    4760
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    123     157     123 
   Size:         34    4750
 
BciVI GTATCCnnnnn_n'

Cuts at:   2682    3443    4784    2682 
   Size:        761    1341    2682

  Fragments arranged by size: 

                2682    1341     761
 
BclI T'GATC_A

Cuts at:   2304    2304 
   Size:       4784
 
BfrBI ATG'CAT

Cuts at:   1496    1496 
   Size:       4784
 
BglII A'GATC_T

Cuts at:   2299    4336    2299 
   Size:       2037    2747
 
BlpI GC'TnA_GC

Cuts at:   1896    1896 
   Size:       4784
 
Bme1580I G_kGCm'C

Cuts at:   2409    2502    3558    2409 
   Size:         93    1056    3635

  Fragments arranged by size: 

                3635    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    320     969    1996    2077    2412    4556     320 
   Size:        649    1027      81     335    2144     548

  Fragments arranged by size: 

                2144    1027     649     548     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    477    1569    3151     477 
   Size:       1092    1582    2110

  Fragments arranged by size: 

                2110    1582    1092
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    482    1094     482 
   Size:        612    4172
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    367    1509    1960    2839    3331    3629     367 
   Size:       1142     451     879     492     298    1522

  Fragments arranged by size: 

                1522    1142     879     492     451     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4488    4488 
   Size:       4784
 
BsaAI yAC'GTr

Cuts at:   2137    2137 
   Size:       4784
 
BsaBI GATnn'nnATC

Cuts at:      6     541    1673    2298    4335       6 
   Size:        535    1132     625    2037     455

  Fragments arranged by size: 

                2037    1132     625     535     455
 
BsaHI Gr'CG_yC

Cuts at:    157    2215    2463    3165    4661     157 
   Size:       2058     248     702    1496     280

  Fragments arranged by size: 

                2058    1496     702     280     248
 
BsaWI w'CCGG_w

Cuts at:   1973    2494    3172    3446    3593    4004    1973 
   Size:        521     678     274     147     411    2753

  Fragments arranged by size: 

                2753     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    194     194 
   Size:       4784
 
BseYI C'CCAG_C

Cuts at:   2209    2967    3544    4040    2209 
   Size:        758     577     496    2953

  Fragments arranged by size: 

                2953     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    2326    2372    3580     104 
   Size:       2222      46    1208    1308

  Fragments arranged by size: 

                2222    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    146    1164    1332    1798    2576    2766    3558     146 
   Size:       1018     168     466     778     190     792    1372

  Fragments arranged by size: 

                1372    1018     792     778     466     190     168
 
Bsp1286I G_dGCh'C

Cuts at:    146    1164    1332    1798    2409    2502    2576    2766    2828 
   Size:       1018     168     466     611      93      74     190      62

Cuts at:   2828    3558     146 
   Size:        730    1372

  Fragments arranged by size: 

                1372    1018     730     611     466     190     168      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   4004    4004 
   Size:       4784
 
BspHI T'CATG_A

Cuts at:   3960    3960 
   Size:       4784
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    172    1834    2048    2350    2731     172 
   Size:       1662     214     302     381    2225

  Fragments arranged by size: 

                2225    1662     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3076    3130    4614    4779      50 
   Size:       3026      54    1484     165      55

  Fragments arranged by size: 

                3026    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2696    2696 
   Size:       4784
 
BsrFI r'CCGG_y

Cuts at:   2782    2963    3172    2782 
   Size:        181     209    4394

  Fragments arranged by size: 

                4394     209     181
 
BssSI C'ACGA_G

Cuts at:   3055    3413    3055 
   Size:        358    4426
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1450    2161    1450 
   Size:        711    4073
 
BstBI TT'CG_AA

Cuts at:   3145    3145 
   Size:       4784
 
BstYI r'GATC_y

Cuts at:      1    1126    1809    2276    2299    2634    2880    3892    3978 
   Size:       1125     683     467      23     335     246    1012      86

Cuts at:   3978    3990    4336       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1125    1012     683     467     449     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    247     247 
   Size:       4784
 
BtgI C'CryG_G

Cuts at:    152     160    1337     152 
   Size:          8    1177    3599

  Fragments arranged by size: 

                3599    1177       8
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    278    2734    2915    3078     278 
   Size:       2456     181     163    1984

  Fragments arranged by size: 

                2456    1984     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1368    2027    1368 
   Size:        659    4125
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    868     903     868 
   Size:         35    4749
 
DraI TTT'AAA

Cuts at:    905    1783     905 
   Size:        878    3906
 
DrdI GACnn_nn'nnGTC

Cuts at:   2490    3348    2490 
   Size:        858    3926
 
EaeI y'GGCC_r

Cuts at:   2369    2543    2934    2961    4306    2369 
   Size:        174     391      27    1345    2847

  Fragments arranged by size: 

                2847    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2369    2369 
   Size:       4784
 
EarI CTCTTCn'nnn_

Cuts at:    630    1061    2807    3017     630 
   Size:        431    1746     210    2397

  Fragments arranged by size: 

                2397    1746     431     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2992    3302    3448    4594    2992 
   Size:        310     146    1146    3182

  Fragments arranged by size: 

                3182    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1796    1796 
   Size:       4784
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    482     539     611    1085    1094    2609    3041    3788    4056 
   Size:         57      72     474       9    1515     432     747     268

Cuts at:   4056     482 
   Size:       1210

  Fragments arranged by size: 

                1515    1210     747     474     432     268      72      57
                   9
 
EcoO109I rG'GnC_Cy

Cuts at:   1923    1923 
   Size:       4784
 
EcoRI G'AATT_C

Cuts at:   1788    1788 
   Size:       4784
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2803    2803 
   Size:       4784
 
FspI TGC'GCA

Cuts at:   2565    2565 
   Size:       4784
 
HaeII r_GCGC'y

Cuts at:    160    2218    2466    3488    4467     160 
   Size:       2058     248    1022     979     477

  Fragments arranged by size: 

                2058    1022     979     477     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    480     512    2626    2656    2658    2688     480 
   Size:         32    2114      30       2      30    2576

  Fragments arranged by size: 

                2576    2114      32      30      30       2
 
HincII GTy'rAC

Cuts at:    222     459    1823     222 
   Size:        237    1364    3183

  Fragments arranged by size: 

                3183    1364     237
 
HindIII A'AGCT_T

Cuts at:    591    1094     591 
   Size:        503    4281
 
KasI G'GCGC_C

Cuts at:    156    2214    2462     156 
   Size:       2058     248    2478

  Fragments arranged by size: 

                2478    2058     248
 
KpnI G_GTAC'C

Cuts at:   1804    1804 
   Size:       4784
 
MfeI C'AATT_G

Cuts at:   1637    1637 
   Size:       4784
 
MluI A'CGCG_T

Cuts at:   4217    4217 
   Size:       4784
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1410    3455    3639    1410 
   Size:       2045     184    2555

  Fragments arranged by size: 

                2555    2045     184
 
MscI TGG'CCA

Cuts at:   2545    2545 
   Size:       4784
 
MslI CAynn'nnrTG

Cuts at:   1186    2773    2900    1186 
   Size:       1587     127    3070

  Fragments arranged by size: 

                3070    1587     127
 
MspA1I CmG'CkG

Cuts at:   1895    2209    2569    3582    3827    1895 
   Size:        314     360    1013     245    2852

  Fragments arranged by size: 

                2852    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2965    2965 
   Size:       4784
 
NarI GG'CG_CC

Cuts at:    157    2215    2463     157 
   Size:       2058     248    2478

  Fragments arranged by size: 

                2478    2058     248
 
NcoI C'CATG_G

Cuts at:    160    1337     160 
   Size:       1177    3607
 
NdeI CA'TA_TG

Cuts at:    861     861 
   Size:       4784
 
NgoMIV G'CCGG_C

Cuts at:   2963    2963 
   Size:       4784
 
NsiI A_TGCA'T

Cuts at:   1498    1498 
   Size:       4784
 
NspI r_CATG'y

Cuts at:   1837    2337    3244    1837 
   Size:        500     907    3377

  Fragments arranged by size: 

                3377     907     500
 
PciI A'CATG_T

Cuts at:   3240    3240 
   Size:       4784
 
PfoI T'CCnGG_A

Cuts at:    549     915    1193     549 
   Size:        366     278    4140

  Fragments arranged by size: 

                4140     366     278
 
PmeI GTTT'AAAC

Cuts at:   1783    1783 
   Size:       4784
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3923    3955    3923 
   Size:         32    4752
 
PsiI TTA'TAA

Cuts at:    677     677 
   Size:       4784
 
PstI C_TGCA'G

Cuts at:   1831    2516    1831 
   Size:        685    4099
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4784
 
PvuII CAG'CTG

Cuts at:   2209    2569    2209 
   Size:        360    4424
 
RsrII CG'GwC_CG

Cuts at:   2979    2979 
   Size:       4784
 
SacI G_AGCT'C

Cuts at:   1798    1798 
   Size:       4784
 
SalI G'TCGA_C

Cuts at:    220    1821     220 
   Size:       1601    3183
 
SapI GCTCTTCn'nnn_

Cuts at:   2807    3017    2807 
   Size:        210    4574
 
SbfI CC_TGCA'GG

Cuts at:   1831    1831 
   Size:       4784
 
ScaI AGT'ACT

Cuts at:   4301    4301 
   Size:       4784
 
SfcI C'TryA_G

Cuts at:     33     750    1643    1762    1827    2512    3505    3696      33 
   Size:        717     893     119      65     685     993     191    1121

  Fragments arranged by size: 

                1121     993     893     717     685     191     119      65
            
 
SfoI GGC'GCC

Cuts at:    158    2216    2464     158 
   Size:       2058     248    2478

  Fragments arranged by size: 

                2478    2058     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4784
 
SmaI CCC'GGG

Cuts at:   1806    1806 
   Size:       4784
 
SmlI C'TyrA_G

Cuts at:    382    1488    1939    2854    3346    3608     382 
   Size:       1106     451     915     492     262    1558

  Fragments arranged by size: 

                1558    1106     915     492     451     262
 
SpeI A'CTAG_T

Cuts at:     67     355      67 
   Size:        288    4496
 
SphI G_CATG'C

Cuts at:   1837    2337    1837 
   Size:        500    4284
 
SspI AAT'ATT

Cuts at:    351     654     351 
   Size:        303    4481
 
StyI C'CwwG_G

Cuts at:    160    1337    1918    2272     160 
   Size:       1177     581     354    2672

  Fragments arranged by size: 

                2672    1177     581     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    153     329    2367    2500    2959    3168     153 
   Size:        176    2038     133     459     209    1769

  Fragments arranged by size: 

                2038    1769     459     209     176     133
 
TatI w'GTAC_w

Cuts at:    504     759    4299     504 
   Size:        255    3540     989

  Fragments arranged by size: 

                3540     989     255
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    729     761    4301    4333     729 
   Size:         32    3540      32    1180

  Fragments arranged by size: 

                3540    1180      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2581    2581 
   Size:       4784
 
XbaI T'CTAG_A

Cuts at:   1815    1815 
   Size:       4784
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2279    2279 
   Size:       4784
 
XmaI C'CCGG_G

Cuts at:   1804    1804 
   Size:       4784
 
XmnI GAAnn'nnTTC

Cuts at:   1483    1719    1483 
   Size:        236    4548
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NsiI     NspI     PciI     PfoI
     PmeI     PpiI     PsiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     SspI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BglI    BmgBI     BmtI   Bpu10I    BsaXI
     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI
  BstZ17I     ClaI   DraIII    EcoNI    EcoRV     FseI    FspAI     HpaI
     NheI     NotI     NruI     PacI     PasI    PflMI     PmlI    PpuMI
    PshAI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI     ZraI
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