Restriction map for pF1KB9969
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BsaBI       BsaI        BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspLU11I    BsrBI       BsrGI       
BssSI       Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HincII      
HindII      HpaI        KpnI        MamI        MfeI        
MluI        MseI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SbfI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tth111I     
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccIII         83  TCCGGA               T^CCGGA
AflIII        533  ACGTGT               A^C[GA][CT]GT
AocI         1219  CCTGAGG              CC^T[ACGT]AGG
AvaII        1523  GGTCC                G^G[AT]CC
AviII        1028  TGCGCA               TGC^GCA
BbsI         1257  GAAGAC               GAAGAC(2/6)
BfaI           77  CTAG                 C^TAG
BpuAI        1257  GAAGAC               GAAGAC(2/6)
BsaAI         532  TACGTG               [CT]AC^GT[GA]
BsaWI          83  TCCGGA               [AT]^CCGG[AT]
BseAI          83  TCCGGA               T^CCGGA
BsmBI         218  GAGACG               CGTCTC(1/5)
BsmFI        1163  GGGAC                GGGAC(10/14)
BspEI          83  TCCGGA               T^CCGGA
BspHI         893  TCATGA               T^CATGA
BsrDI        1118  GCAATG               GCAATG(2/0)
BsrI          123  ACTGG                ACTGG(1/-1)
BssHII        714  GCGCGC               G^CGCGC
BstEII       1195  GGTGACC              G^GT[ACGT]ACC
BstPI        1195  GGTGACC              G^GT[ACGT]ACC
BstXI        1152  CCACGCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1219  CCTGAGG              CC^T[ACGT]AGG
Eco81I       1219  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1374  CCTGGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1195  GGTGACC              G^GT[ACGT]ACC
FspI         1028  TGCGCA               TGC^GCA
HindIII       427  AAGCTT               A^AGCTT
KspI          623  CCGCGG               CCGC^GG
MaeI           77  CTAG                 C^TAG
MroI           83  TCCGGA               T^CCGGA
NaeI         1105  GCCGGC               GCC^GGC
NgoMIV       1105  GCCGGC               G^CCGGC
PaeR7I       1089  CTCGAG               C^TCGAG
PflMI        1497  CCAGGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         403  GACAAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          893  TCATGA               T^CATGA
SacI         1092  GAGCTC               GAGCT^C
SacII         623  CCGCGG               CCGC^GG
ScaI          467  AGTACT               AGT^ACT
SexAI         791  ACCTGGT              A^CC[AT]GGT
SmlI         1089  CTCGAG               C^T[CT][GA]AG
StuI          683  AGGCCT               AGG^CCT
Van91I       1497  CCAGGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          782  CCAAGTCCAACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1089  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AfaI          468  GTAC                 GT^AC
              489  GTAC                
AlwI          255  GATCC                GGATC(4/5)
              705  GATCC               
AlwNI         661  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1498  CAGGGCCTG           
BanII         201  GGGCTC               G[GA]GC[CT]^C
             1092  GAGCTC              
BbeI          751  GGCGCC               GGCGC^C
             1414  GGCGCC              
BlpI          415  GCTGAGC              GC^T[ACGT]AGC
              996  GCTCAGC             
Bpu1102I      415  GCTGAGC              GC^T[ACGT]AGC
              996  GCTCAGC             
BsgI         1327  CTGCAC               GTGCAG(16/14)
              721  GTGCAG              
BspMI         127  GCAGGT               ACCTGC(4/8)
              735  GCAGGT              
BstAPI        660  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              723  GCAGCTCATGC         
BstYI         254  AGATCC               [GA]^GATC[CT]
              704  AGATCC              
CelII         415  GCTGAGC              GC^T[ACGT]AGC
              996  GCTCAGC             
DraII        1465  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1499  AGGGCCT             
EcoO109I     1465  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1499  AGGGCCT             
KasI          751  GGCGCC               G^GCGCC
             1414  GGCGCC              
MflI          254  AGATCC               [GA]^GATC[CT]
              704  AGATCC              
MslI           93  CATCGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1621  CACGGGGATG          
NarI          751  GGCGCC               GG^CGCC
             1414  GGCGCC              
NcoI           98  CCATGG               C^CATGG
             1097  CCATGG              
PstI          484  CTGCAG               CTGCA^G
             1339  CTGCAG              
RsaI          468  GTAC                 GT^AC
              489  GTAC                
SapI          438  GAAGAGC              GCTCTTC(1/4)
             1350  GAAGAGC             
SfaNI          24  GATGC                GCATC(5/9)
             1266  GATGC               
SfoI          751  GGCGCC               GGC^GCC
             1414  GGCGCC              
SmaI         1295  CCCGGG               CCC^GGG
             1424  CCCGGG              
TaqI          494  TCGA                 T^CGA
             1090  TCGA                
Tsp509I       109  AATT                 ^AATT
              529  AATT                
TthHB8I       494  TCGA                 T^CGA
             1090  TCGA                
XhoII         254  AGATCC               [GA]^GATC[CT]
              704  AGATCC              
XmaCI        1295  CCCGGG               C^CCGGG
             1424  CCCGGG              
XmaI         1295  CCCGGG               C^CCGGG
             1424  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AspHI       
AvaI        BalI        BanI        BbvI        BcnI        
BglI        BmyI        BpmI        BsaHI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsoBI       Bsp1286I    BsrFI       BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EarI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PvuII       Sau3AI      Sau96I      ScrFI       SfcI        
StyI        TfiI        TseI        Tsp45I      TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com