Restriction map for pF1KB9969
 (Linear) (Six-base) MAPSORT of: KIBB9969.seq  Check: 3611  from: 1  to: 1656



KIBB9969.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     118     726    1656 
   Size:        118     608     930

  Fragments arranged by size: 

                 930     608     118
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     301     379     442    1579    1656 
   Size:        260      41      78      63    1137      77

  Fragments arranged by size: 

                1137     260      78      77      63      41
 
AflIII A'CryG_T

Cuts at:      0     533    1656 
   Size:        533    1123
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     667    1656 
   Size:        667     989
 
AlwNI CAG_nnn'CTG

Cuts at:      0     666    1503    1656 
   Size:        666     837     153

  Fragments arranged by size: 

                 837     666     153
 
AvaI C'yCGr_G

Cuts at:      0     841    1089    1295    1424    1656 
   Size:        841     248     206     129     232

  Fragments arranged by size: 

                 841     248     232     206     129
 
BanI G'GyrC_C

Cuts at:      0     751     834     849    1215    1414    1656 
   Size:        751      83      15     366     199     242

  Fragments arranged by size: 

                 751     366     242     199      83      15
 
BanII G_rGCy'C

Cuts at:      0     205    1096    1656 
   Size:        205     891     560

  Fragments arranged by size: 

                 891     560     205
 
BbeI G_GCGC'C

Cuts at:      0     755    1418    1656 
   Size:        755     663     238

  Fragments arranged by size: 

                 755     663     238
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1264    1656 
   Size:       1264     392
 
BglI GCCn_nnn'nGGC

Cuts at:      0     675     775     917    1426    1444    1656 
   Size:        675     100     142     509      18     212

  Fragments arranged by size: 

                 675     509     212     142     100      18
 
BlpI GC'TnA_GC

Cuts at:      0     416     997    1656 
   Size:        416     581     659

  Fragments arranged by size: 

                 659     581     416
 
Bme1580I G_kGCm'C

Cuts at:      0     839    1487    1656 
   Size:        839     648     169

  Fragments arranged by size: 

                 839     648     169
 
BmgBI CAC'GTC

Cuts at:      0    1551    1656 
   Size:       1551     105
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     241     351    1656 
   Size:        241     110    1305

  Fragments arranged by size: 

                1305     241     110
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     321     334     544    1132    1656 
   Size:        321      13     210     588     524

  Fragments arranged by size: 

                 588     524     321     210      13
 
Bpu10I CC'TnA_GC

Cuts at:      0    1180    1656 
   Size:       1180     476
 
BsaAI yAC'GTr

Cuts at:      0     534    1656 
   Size:        534    1122
 
BsaHI Gr'CG_yC

Cuts at:      0     161     395     497     752     902    1415    1656 
   Size:        161     234     102     255     150     513     241

  Fragments arranged by size: 

                 513     255     241     234     161     150     102
 
BsaWI w'CCGG_w

Cuts at:      0      83    1656 
   Size:         83    1573
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     472     502     586     616     994    1024    1656 
   Size:        472      30      84      30     378      30     632

  Fragments arranged by size: 

                 632     472     378      84      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34     580     965    1656 
   Size:         34     546     385     691

  Fragments arranged by size: 

                 691     546     385      34
 
BseYI C'CCAG_C

Cuts at:      0     697     763     956    1159    1656 
   Size:        697      66     193     203     497

  Fragments arranged by size: 

                 697     497     203     193      66
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     742    1312    1656 
   Size:        742     570     344

  Fragments arranged by size: 

                 742     570     344
 
BsiHKAI G_wGCw'C

Cuts at:      0     886    1096    1622    1656 
   Size:        886     210     526      34

  Fragments arranged by size: 

                 886     526     210      34
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     212    1656 
   Size:        212    1444
 
Bsp1286I G_dGCh'C

Cuts at:      0     205     839     886    1096    1487    1622    1656 
   Size:        205     634      47     210     391     135      34

  Fragments arranged by size: 

                 634     391     210     205     135      47      34
 
BspEI T'CCGG_A

Cuts at:      0      83    1656 
   Size:         83    1573
 
BspHI T'CATG_A

Cuts at:      0     893    1656 
   Size:        893     763
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     118     726    1656 
   Size:        118     608     930

  Fragments arranged by size: 

                 930     608     118
 
BsrDI GCAATG_nn'

Cuts at:      0    1125    1656 
   Size:       1125     531
 
BsrFI r'CCGG_y

Cuts at:      0      29     831    1105    1656 
   Size:         29     802     274     551

  Fragments arranged by size: 

                 802     551     274      29
 
BssHII G'CGCG_C

Cuts at:      0     714    1656 
   Size:        714     942
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     666     729    1656 
   Size:        666      63     927

  Fragments arranged by size: 

                 927     666      63
 
BstEII G'GTnAC_C

Cuts at:      0    1195    1656 
   Size:       1195     461
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1159    1656 
   Size:       1159     497
 
BstYI r'GATC_y

Cuts at:      0     254     704    1656 
   Size:        254     450     952

  Fragments arranged by size: 

                 952     450     254
 
Bsu36I CC'TnA_GG

Cuts at:      0    1220    1656 
   Size:       1220     436
 
BtgI C'CryG_G

Cuts at:      0      98     608     623     934    1097    1656 
   Size:         98     510      15     311     163     559

  Fragments arranged by size: 

                 559     510     311     163      98      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      78    1244    1325    1656 
   Size:         78    1166      81     331

  Fragments arranged by size: 

                1166     331      81      78
 
BtsI GCAGTG_nn'

Cuts at:      0     607    1323    1656 
   Size:        607     716     333

  Fragments arranged by size: 

                 716     607     333
 
EaeI y'GGCC_r

Cuts at:      0     185     611     740    1122    1391    1445    1478    1656 
   Size:        185     426     129     382     269      54      33     178

  Fragments arranged by size: 

                 426     382     269     185     178     129      54      33
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     232     433    1345    1656 
   Size:        232     201     912     311

  Fragments arranged by size: 

                 912     311     232     201
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     879    1584    1656 
   Size:        879     705      72

  Fragments arranged by size: 

                 879     705      72
 
EcoICRI GAG'CTC

Cuts at:      0    1094    1656 
   Size:       1094     562
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     301     321     334     379     442     544    1132 
   Size:        260      41      20      13      45      63     102     588

Cuts at:   1132    1579    1656 
   Size:        447      77

  Fragments arranged by size: 

                 588     447     260     102      77      63      45      41
                  20      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1378    1656 
   Size:       1378     278
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1466    1500    1656 
   Size:       1466      34     156

  Fragments arranged by size: 

                1466     156      34
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1161    1656 
   Size:       1161     495
 
FspI TGC'GCA

Cuts at:      0    1030    1656 
   Size:       1030     626
 
HaeII r_GCGC'y

Cuts at:      0     579     755     770    1418    1637    1656 
   Size:        579     176      15     648     219      19

  Fragments arranged by size: 

                 648     579     219     176      19      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     246     278     994    1026    1068    1100    1521    1553 
   Size:        246      32     716      32      42      32     421      32

Cuts at:   1553    1656 
   Size:        103

  Fragments arranged by size: 

                 716     421     246     103      42      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     427    1656 
   Size:        427    1229
 
KasI G'GCGC_C

Cuts at:      0     751    1414    1656 
   Size:        751     663     242

  Fragments arranged by size: 

                 751     663     242
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     563     812     827    1037    1656 
   Size:        563     249      15     210     619

  Fragments arranged by size: 

                 619     563     249     210      15
 
MscI TGG'CCA

Cuts at:      0     187     613     742    1393    1656 
   Size:        187     426     129     651     263

  Fragments arranged by size: 

                 651     426     263     187     129
 
MslI CAynn'nnrTG

Cuts at:      0      97    1625    1656 
   Size:         97    1528      31

  Fragments arranged by size: 

                1528      97      31
 
MspA1I CmG'CkG

Cuts at:      0     267     312     625     672    1451    1557    1656 
   Size:        267      45     313      47     779     106      99

  Fragments arranged by size: 

                 779     313     267     106      99      47      45
 
NaeI GCC'GGC

Cuts at:      0    1107    1656 
   Size:       1107     549
 
NarI GG'CG_CC

Cuts at:      0     752    1415    1656 
   Size:        752     663     241

  Fragments arranged by size: 

                 752     663     241
 
NcoI C'CATG_G

Cuts at:      0      98    1097    1656 
   Size:         98     999     559

  Fragments arranged by size: 

                 999     559      98
 
NgoMIV G'CCGG_C

Cuts at:      0    1105    1656 
   Size:       1105     551
 
PasI CC'CwG_GG

Cuts at:      0    1497    1656 
   Size:       1497     159
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1503    1656 
   Size:       1503     153
 
PfoI T'CCnGG_A

Cuts at:      0     707    1373    1656 
   Size:        707     666     283

  Fragments arranged by size: 

                 707     666     283
 
PshAI GACnn'nnGTC

Cuts at:      0     407    1656 
   Size:        407    1249
 
PspXI vC'TCGA_Gb

Cuts at:      0    1089    1656 
   Size:       1089     567
 
PstI C_TGCA'G

Cuts at:      0     488    1343    1656 
   Size:        488     855     313

  Fragments arranged by size: 

                 855     488     313
 
PvuII CAG'CTG

Cuts at:      0     267     312    1557    1656 
   Size:        267      45    1245      99

  Fragments arranged by size: 

                1245     267      99      45
 
SacI G_AGCT'C

Cuts at:      0    1096    1656 
   Size:       1096     560
 
SacII CC_GC'GG

Cuts at:      0     626    1656 
   Size:        626    1030
 
SapI GCTCTTCn'nnn_

Cuts at:      0     433    1345    1656 
   Size:        433     912     311

  Fragments arranged by size: 

                 912     433     311
 
ScaI AGT'ACT

Cuts at:      0     469    1656 
   Size:        469    1187
 
SexAI A'CCwGG_T

Cuts at:      0     791    1656 
   Size:        791     865
 
SfcI C'TryA_G

Cuts at:      0     484     927    1339    1470    1656 
   Size:        484     443     412     131     186

  Fragments arranged by size: 

                 484     443     412     186     131
 
SfoI GGC'GCC

Cuts at:      0     753    1416    1656 
   Size:        753     663     240

  Fragments arranged by size: 

                 753     663     240
 
SmaI CCC'GGG

Cuts at:      0    1297    1426    1656 
   Size:       1297     129     230

  Fragments arranged by size: 

                1297     230     129
 
SmlI C'TyrA_G

Cuts at:      0    1089    1656 
   Size:       1089     567
 
StuI AGG'CCT

Cuts at:      0     685    1656 
   Size:        685     971
 
StyI C'CwwG_G

Cuts at:      0      98     638    1097    1599    1656 
   Size:         98     540     459     502      57

  Fragments arranged by size: 

                 540     502     459      98      57
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     903    1656 
   Size:        903     753
 
TatI w'GTAC_w

Cuts at:      0     467     488    1656 
   Size:        467      21    1168

  Fragments arranged by size: 

                1168     467      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     586     618    1656 
   Size:        586      32    1038

  Fragments arranged by size: 

                1038     586      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     789    1656 
   Size:        789     867
 
XhoI C'TCGA_G

Cuts at:      0    1089    1656 
   Size:       1089     567
 
XmaI C'CCGG_G

Cuts at:      0    1295    1424    1656 
   Size:       1295     129     232

  Fragments arranged by size: 

                1295     232     129
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI    AlwNI     AvaI     BanI    BanII
     BbeI     BbsI     BglI     BlpI Bme1580I    BmgBI     BplI     BpmI
   Bpu10I    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI
   BssHII   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI     FspI    HaeII    Hin4I  HindIII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PasI    PflMI
     PfoI    PshAI    PspXI     PstI    PvuII     SacI    SacII     SapI
     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BmrI     BmtI    BpuEI     BsaI    BsaBI    BsiEI    BsiWI
     BsmI    BsrBI    BsrGI    BssSI    BstBI  BstZ17I     ClaI    CspCI
     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV     FseI    FspAI
   HincII     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI
    PpuMI     PsiI   PspOMI     PsrI     PvuI    RsrII     SalI    SanDI
     SbfI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI  Tth111I     XbaI     XmnI     ZraI
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