Restriction map for pF1KB9959
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AccI        AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BbrPI       BciVI       
BclI        BfrI        BglII       BlnI        BsaAI       
BsaBI       BseAI       BsiWI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        Eam1105I    Eco47III    EcoNI       EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
Tru9I       Tsp509I     Tth111I     XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII         850  GACGTC               GACGT^C
Acc65I        815  GGTACC               G^GTACC
AccII        1015  CGCG                 CG^CG
AlwNI        1165  CAGACGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          896  CCTCAGG              CC^T[ACGT]AGG
Asp718I       815  GGTACC               G^GTACC
BbeI         1327  GGCGCC               GGCGC^C
BbvCI         496  GCTGAGG              CCTCAGC(-5/-2)
BcgI           41  CGACGCCGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1480  CTAG                 C^TAG
BlpI         1018  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1018  GCTGAGC              GC^T[ACGT]AGC
BsaI         1042  GAGACC               GGTCTC(1/5)
BsaWI          85  ACCGGA               [AT]^CCGG[AT]
BsgI          984  CTGCAC               GTGCAG(16/14)
BsiEI         822  CGGCCG               CG[GA][CT]^CG
BsmBI         315  CGTCTC               CGTCTC(1/5)
Bsp1407I      188  TGTACA               T^GTACA
BsrBI         301  GAGCGG               CCGCTC(-3/-3)
BsrDI        1107  CATTGC               GCAATG(2/0)
BsrFI        1145  GCCGGC               [GA]^CCGG[CT]
BsrGI         188  TGTACA               T^GTACA
BstUI        1015  CGCG                 CG^CG
Bsu36I        896  CCTCAGG              CC^T[ACGT]AGG
CelII        1018  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1145  GCCGGC               [GA]^CCGG[CT]
DraIII        949  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          618  GACTCAGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          822  CGGCCG               C^GGCCG
EclXI         822  CGGCCG               C^GGCCG
Eco52I        822  CGGCCG               C^GGCCG
Eco81I        896  CCTCAGG              CC^T[ACGT]AGG
KasI         1327  GGCGCC               G^GCGCC
KpnI          815  GGTACC               GGTAC^C
MaeI         1480  CTAG                 C^TAG
MvnI         1015  CGCG                 CG^CG
NaeI         1145  GCCGGC               GCC^GGC
NarI         1327  GGCGCC               GG^CGCC
NgoMIV       1145  GCCGGC               G^CCGGC
PstI         1171  CTGCAG               CTGCA^G
PvuII         937  CAGCTG               CAG^CTG
SacI          355  GAGCTC               GAGCT^C
SapI         1269  GCTCTTC              GCTCTTC(1/4)
SfoI         1327  GGCGCC               GGC^GCC
SmlI         1303  CTCAAG               C^T[CT][GA]AG
SspBI         188  TGTACA               T^GTACA
TfiI         1567  GATTC                G^A[AT]TC
XbaI         1479  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwI          578  GGATC                GGATC(4/5)
             1476  GGATC               
BbsI         1192  GAAGAC               GAAGAC(2/6)
             1462  GAAGAC              
BglI          367  GCCACGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1440  GCCTCCAGGGC         
BpuAI        1192  GAAGAC               GAAGAC(2/6)
             1462  GAAGAC              
BstAPI       1164  GCAGACGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1344  GCAGGAAATGC         
BstEII        201  GGTGACC              G^GT[ACGT]ACC
              705  GGTCACC             
BstPI         201  GGTGACC              G^GT[ACGT]ACC
              705  GGTCACC             
BstXI         995  CCAATGCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1412  CCACAAGCCTGG        
BstYI         578  GGATCT               [GA]^GATC[CT]
             1476  GGATCT              
EarI         1270  CTCTTC               CTCTTC(1/4)
             1507  GAAGAG              
EcoO65I       201  GGTGACC              G^GT[ACGT]ACC
              705  GGTCACC             
HaeII         212  AGCGCC               [GA]GCGC^[CT]
             1327  GGCGCC              
Ksp632I      1270  CTCTTC               CTCTTC(1/4)
             1507  GAAGAG              
MflI          578  GGATCT               [GA]^GATC[CT]
             1476  GGATCT              
PflMI         237  CCAGAATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              948  CCACGCAGTGG         
PleI          618  GACTC                GAGTC(4/5)
              470  GAGTC               
SfcI         1171  CTGCAG               C^T[GA][CT]AG
             1551  CTATAG              
SmaI            5  CCCGGG               CCC^GGG
             1598  CCCGGG              
Van91I        237  CCAGAATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              948  CCACGCAGTGG         
XhoII         578  GGATCT               [GA]^GATC[CT]
             1476  GGATCT              
XmaCI           5  CCCGGG               C^CCGGG
             1598  CCCGGG              
XmaI            5  CCCGGG               C^CCGGG
             1598  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AspHI       
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaHI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     
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