Restriction map for pF1KB9955
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AgeI        Alw44I      AlwNI       AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BcgI        BfrI        BlnI        BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsmI        BspDI       BspEI       BspLU11I    
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraIII      DsaI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HaeII       HgaI        Hin1I       HindIII     HpaI        
KasI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacII       SalI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    StuI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AflIII       1343  ACGTGT               A^C[GA][CT]GT
AlwI         1497  GATCC                GGATC(4/5)
ApaI          653  GGGCCC               GGGCC^C
AspI          592  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI         106  CCTCAGC              CCTCAGC(-5/-2)
BciVI         634  GGATAC               GTATCC(6/5)
BclI          818  TGATCA               T^GATCA
BglI         1300  GCCCCCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1167  AGATCT               A^GATCT
BlpI          475  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      475  GCTGAGC              GC^T[ACGT]AGC
BsiWI         229  CGTACG               C^GTACG
BsmBI         209  CGTCTC               CGTCTC(1/5)
Bsp1407I      681  TGTACA               T^GTACA
BsrBI         366  CCGCTC               CCGCTC(-3/-3)
BsrDI          12  GCAATG               GCAATG(2/0)
BsrGI         681  TGTACA               T^GTACA
BstEII       1377  GGTCACC              G^GT[ACGT]ACC
BstPI        1377  GGTCACC              G^GT[ACGT]ACC
BstXI          69  CCAGAGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1167  AGATCT               [GA]^GATC[CT]
CelII         475  GCTGAGC              GC^T[ACGT]AGC
DrdI          970  GACAAGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          462  TGGCCG               [CT]^GGCC[GA]
EcoO65I      1377  GGTCACC              G^GT[ACGT]ACC
FbaI          818  TGATCA               T^GATCA
HincII        421  GTTGAC               GT[CT]^[GA]AC
HindII        421  GTTGAC               GT[CT]^[GA]AC
MaeII        1343  ACGT                 A^CGT
MflI         1167  AGATCT               [GA]^GATC[CT]
MslI         1185  CATTCTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI         1206  CATATG               CA^TATG
PaeR7I        800  CTCGAG               C^TCGAG
PflFI         592  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1286  CCATCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI         1076  GAAGAGC              GCTCTTC(1/4)
ScaI         1541  AGTACT               AGT^ACT
SexAI        1596  ACCTGGT              A^CC[AT]GGT
SmaI          650  CCCGGG               CCC^GGG
SspBI         681  TGTACA               T^GTACA
SspI          330  AATATT               AAT^ATT
Tth111I       592  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1286  CCATCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          800  CTCGAG               C^TCGAG
XhoII        1167  AGATCT               [GA]^GATC[CT]
XmaCI         650  CCCGGG               C^CCGGG
XmaI          650  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccII         526  CGCG                 CG^CG
             1575  CGCG                
AcsI          322  AAATTT               [GA]^AATT[CT]
              611  AAATTC              
AhdI          179  GACTCACCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1342  GACGTGTGGTC         
ApoI          322  AAATTT               [GA]^AATT[CT]
              611  AAATTC              
Asp700I       319  GAAAAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              664  GAAACACTTC          
AspEI         179  GACTCACCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1342  GACGTGTGGTC         
BbsI         1246  GAAGAC               GAAGAC(2/6)
             1561  GAAGAC              
BfaI          724  CTAG                 C^TAG
             1189  CTAG                
BpuAI        1246  GAAGAC               GAAGAC(2/6)
             1561  GAAGAC              
BsaI          590  GAGACC               GGTCTC(1/5)
              709  GGTCTC              
BspHI         950  TCATGA               T^CATGA
             1493  TCATGA              
BspMI         706  GCAGGT               ACCTGC(4/8)
             1431  GCAGGT              
BstUI         526  CGCG                 CG^CG
             1575  CGCG                
Cac8I         364  GCCCGC               GC[ACGT]^[ACGT]GC
             1460  GCCCGC              
CfoI          943  GCGC                 GCG^C
             1146  GCGC                
DraII         220  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1514  AGGACCC             
Eam1105I      179  GACTCACCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1342  GACGTGTGGTC         
EcoO109I      220  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1514  AGGACCC             
EcoT14I       174  CCAAGG               C^C[AT][AT]GG
              751  CCAAGG              
FauI          365  CCCGC                CCCGC(4/6)
             1461  CCCGC               
HhaI          943  GCGC                 GCG^C
             1146  GCGC                
HinP1I        943  GCGC                 G^CGC
             1146  GCGC                
MaeI          724  CTAG                 C^TAG
             1189  CTAG                
MseI          902  TTAA                 T^TAA
             1060  TTAA                
MvnI          526  CGCG                 CG^CG
             1575  CGCG                
PpuMI         220  GGGACCT              [GA]G^G[AT]CC[CT]
             1514  AGGACCC             
RcaI          950  TCATGA               T^CATGA
             1493  TCATGA              
SmlI          800  CTCGAG               C^T[CT][GA]AG
              934  CTTGAG              
StyI          174  CCAAGG               C^C[AT][AT]GG
              751  CCAAGG              
TaqI          801  TCGA                 T^CGA
             1142  TCGA                
Tru9I         902  TTAA                 T^TAA
             1060  TTAA                
TspRI         992  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1501  CACTG               
TthHB8I       801  TCGA                 T^CGA
             1142  TCGA                
XcmI          401  CCAGCAATTATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1587  CCAGTACCAACCTGG     
XmnI          319  GAAAAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              664  GAAACACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

Acc65I      AciI        AfaI        AluI        Asp718I     
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
KpnI        Ksp632I     MaeIII      MboI        MboII       
MnlI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TfiI        TseI        Tsp45I      
Tsp509I     
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