Restriction map for pF1KB9918
 (Linear) (Six-base) MAPSORT of: KIBB9918.seq  Check: 5609  from: 1  to: 1737



KIBB9918.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AatII G_ACGT'C

Cuts at:      0     635    1737 
   Size:        635    1102
 
AccI GT'mk_AC

Cuts at:      0       4    1737 
   Size:          4    1733
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     614     621    1737 
   Size:        341     273       7    1116

  Fragments arranged by size: 

                1116     341     273       7
 
AfeI AGC'GCT

Cuts at:      0     452    1039    1737 
   Size:        452     587     698

  Fragments arranged by size: 

                 698     587     452
 
AflIII A'CryG_T

Cuts at:      0     670    1172    1737 
   Size:        670     502     565

  Fragments arranged by size: 

                 670     565     502
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1031    1737 
   Size:       1031     706
 
AlwNI CAG_nnn'CTG

Cuts at:      0      13    1267    1737 
   Size:         13    1254     470

  Fragments arranged by size: 

                1254     470      13
 
ApoI r'AATT_y

Cuts at:      0     515     759    1737 
   Size:        515     244     978

  Fragments arranged by size: 

                 978     515     244
 
BanI G'GyrC_C

Cuts at:      0     588    1737 
   Size:        588    1149
 
BanII G_rGCy'C

Cuts at:      0     331    1737 
   Size:        331    1406
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      29     627    1737 
   Size:         29     598    1110

  Fragments arranged by size: 

                1110     598      29
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     908    1737 
   Size:        908     829
 
Bme1580I G_kGCm'C

Cuts at:      0    1459    1737 
   Size:       1459     278
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1292    1737 
   Size:       1292     445
 
Bpu10I CC'TnA_GC

Cuts at:      0     767    1737 
   Size:        767     970
 
BsaHI Gr'CG_yC

Cuts at:      0     632    1737 
   Size:        632    1105
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      12      15      18      21     146    1737 
   Size:         12       3       3       3     125    1591

  Fragments arranged by size: 

                1591     125      12       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     641     702     815    1737 
   Size:        641      61     113     922

  Fragments arranged by size: 

                 922     641     113      61
 
BsiEI CG_ry'CG

Cuts at:      0      44    1737 
   Size:         44    1693
 
Bsp1286I G_dGCh'C

Cuts at:      0     331    1459    1737 
   Size:        331    1128     278

  Fragments arranged by size: 

                1128     331     278
 
BsrDI GCAATG_nn'

Cuts at:      0     361     395     962    1684    1737 
   Size:        361      34     567     722      53

  Fragments arranged by size: 

                 722     567     361      53      34
 
BsrFI r'CCGG_y

Cuts at:      0      43    1737 
   Size:         43    1694
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1410    1737 
   Size:       1410     327
 
BstYI r'GATC_y

Cuts at:      0     807    1231    1737 
   Size:        807     424     506

  Fragments arranged by size: 

                 807     506     424
 
Bsu36I CC'TnA_GG

Cuts at:      0    1320    1737 
   Size:       1320     417
 
BtgI C'CryG_G

Cuts at:      0    1113    1357    1737 
   Size:       1113     244     380

  Fragments arranged by size: 

                1113     380     244
 
BtsI GCAGTG_nn'

Cuts at:      0    1128    1487    1737 
   Size:       1128     359     250

  Fragments arranged by size: 

                1128     359     250
 
EaeI y'GGCC_r

Cuts at:      0      41    1737 
   Size:         41    1696
 
EagI C'GGCC_G

Cuts at:      0      41    1737 
   Size:         41    1696
 
EarI CTCTTCn'nnn_

Cuts at:      0      80     110     123    1737 
   Size:         80      30      13    1614

  Fragments arranged by size: 

                1614      80      30      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     614     621    1737 
   Size:        341     273       7    1116

  Fragments arranged by size: 

                1116     341     273       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1621    1737 
   Size:       1621     116
 
EcoO109I rG'GnC_Cy

Cuts at:      0      72     465    1737 
   Size:         72     393    1272

  Fragments arranged by size: 

                1272     393      72
 
HaeII r_GCGC'y

Cuts at:      0     454     822    1041    1737 
   Size:        454     368     219     696

  Fragments arranged by size: 

                 696     454     368     219
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     657     689    1737 
   Size:        657      32    1048

  Fragments arranged by size: 

                1048     657      32
 
HindIII A'AGCT_T

Cuts at:      0     271    1098    1636    1737 
   Size:        271     827     538     101

  Fragments arranged by size: 

                 827     538     271     101
 
MluI A'CGCG_T

Cuts at:      0     670    1737 
   Size:        670    1067
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     655    1737 
   Size:        105     550    1082

  Fragments arranged by size: 

                1082     550     105
 
MslI CAynn'nnrTG

Cuts at:      0    1000    1408    1737 
   Size:       1000     408     329

  Fragments arranged by size: 

                1000     408     329
 
MspA1I CmG'CkG

Cuts at:      0     641    1737 
   Size:        641    1096
 
NcoI C'CATG_G

Cuts at:      0    1357    1737 
   Size:       1357     380
 
NspI r_CATG'y

Cuts at:      0     441    1176    1737 
   Size:        441     735     561

  Fragments arranged by size: 

                 735     561     441
 
PciI A'CATG_T

Cuts at:      0    1172    1737 
   Size:       1172     565
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     420    1737 
   Size:        420    1317
 
PfoI T'CCnGG_A

Cuts at:      0     873    1737 
   Size:        873     864
 
PpuMI rG'GwC_Cy

Cuts at:      0      72     465    1737 
   Size:         72     393    1272

  Fragments arranged by size: 

                1272     393      72
 
PvuII CAG'CTG

Cuts at:      0     641    1737 
   Size:        641    1096
 
SexAI A'CCwGG_T

Cuts at:      0    1266    1737 
   Size:       1266     471
 
SfcI C'TryA_G

Cuts at:      0       5    1438    1737 
   Size:          5    1433     299

  Fragments arranged by size: 

                1433     299       5
 
SpeI A'CTAG_T

Cuts at:      0    1566    1737 
   Size:       1566     171
 
SphI G_CATG'C

Cuts at:      0     441    1737 
   Size:        441    1296
 
SspI AAT'ATT

Cuts at:      0     264    1737 
   Size:        264    1473
 
StyI C'CwwG_G

Cuts at:      0     414    1357    1737 
   Size:        414     943     380

  Fragments arranged by size: 

                 943     414     380
 
TatI w'GTAC_w

Cuts at:      0     943    1737 
   Size:        943     794
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1473    1737 
   Size:       1473     264
 
ZraI GAC'GTC

Cuts at:      0     633    1737 
   Size:        633    1104
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI   AflIII     AlfI    AlwNI     ApoI
     BanI    BanII     BbsI    BciVI Bme1580I     BmrI   Bpu10I    BsaHI
    BseRI    BseYI    BsiEI Bsp1286I    BsrDI    BsrFI    BstXI    BstYI
   Bsu36I     BtgI     BtsI     EaeI     EagI     EarI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I  HindIII     MluI     MmeI     MslI   MspA1I
     NcoI     NspI     PciI    PflMI     PfoI    PpuMI    PvuII    SexAI
     SfcI     SpeI     SphI     SspI     StyI     TatI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII     HpaI     KasI     KpnI     MfeI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI
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