(Circular) (Six-base) MAPSORT of: pF1KB9910.seq Check: 3457 from: 1 to: 4883 pF1KB9910 4883 bp With 182 enzymes: SgfI * April 24, 2009 13:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1495 1622 1495 Size: 127 4756 AatII G_ACGT'C Cuts at: 1727 1727 Size: 4883 AccI GT'mk_AC Cuts at: 1921 1921 Size: 4883 Acc65I G'GTAC_C Cuts at: 656 1798 1899 656 Size: 1142 101 3640 Fragments arranged by size: 3640 1142 101 AclI AA'CG_TT Cuts at: 1877 4723 1877 Size: 2846 2037 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 325 539 617 1276 1411 2708 3140 3887 4155 Size: 214 78 659 135 1297 432 747 268 Cuts at: 4155 325 Size: 1053 Fragments arranged by size: 1297 1053 747 659 432 268 214 135 78 AfeI AGC'GCT Cuts at: 907 907 Size: 4883 AflIII A'CryG_T Cuts at: 815 2235 3339 4316 815 Size: 1420 1104 977 1382 Fragments arranged by size: 1420 1382 1104 977 AgeI A'CCGG_T Cuts at: 3271 3271 Size: 4883 AleI CACnn'nnGTG Cuts at: 373 676 1714 373 Size: 303 1038 3542 Fragments arranged by size: 3542 1038 303 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 926 926 Size: 4883 AlwNI CAG_nnn'CTG Cuts at: 983 1487 1500 1515 2188 3755 983 Size: 504 13 15 673 1567 2111 Fragments arranged by size: 2111 1567 673 504 15 13 ApaLI G'TGCA_C Cuts at: 3653 3653 Size: 4883 ApoI r'AATT_y Cuts at: 256 1887 4236 4492 256 Size: 1631 2349 256 647 Fragments arranged by size: 2349 1631 647 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4883 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4883 AvaI C'yCGr_G Cuts at: 266 711 994 1903 266 Size: 445 283 909 3246 Fragments arranged by size: 3246 909 445 283 BamHI G'GATC_C Cuts at: 1908 1908 Size: 4883 BanI G'GyrC_C Cuts at: 127 656 1798 1899 2313 2561 2596 127 Size: 529 1142 101 414 248 35 2414 Fragments arranged by size: 2414 1142 529 414 248 101 35 BanII G_rGCy'C Cuts at: 874 1020 1146 1897 2927 874 Size: 146 126 751 1030 2830 Fragments arranged by size: 2830 1030 751 146 126 BbeI G_GCGC'C Cuts at: 131 2317 2565 131 Size: 2186 248 2449 Fragments arranged by size: 2449 2186 248 BbsI GAAGACnn'nnnn_ Cuts at: 1594 1594 Size: 4883 BciVI GTATCCnnnnn_n' Cuts at: 2781 3542 4883 2781 Size: 761 1341 2781 Fragments arranged by size: 2781 1341 761 BclI T'GATC_A Cuts at: 476 2403 476 Size: 1927 2956 BglI GCCn_nnn'nGGC Cuts at: 988 988 Size: 4883 BglII A'GATC_T Cuts at: 2398 4435 2398 Size: 2037 2846 BlpI GC'TnA_GC Cuts at: 1995 1995 Size: 4883 Bme1580I G_kGCm'C Cuts at: 961 2508 2601 3657 961 Size: 1547 93 1056 2187 Fragments arranged by size: 2187 1547 1056 93 BmgBI CAC'GTC Cuts at: 1563 1563 Size: 4883 BmrI ACTGGGnnnn_n' Cuts at: 2095 2176 2511 4655 2095 Size: 81 335 2144 2323 Fragments arranged by size: 2323 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3250 3250 Size: 4883 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 230 317 1687 1798 230 Size: 87 1370 111 3315 Fragments arranged by size: 3315 1370 111 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1597 2059 2938 3430 3728 1597 Size: 462 879 492 298 2752 Fragments arranged by size: 2752 879 492 462 298 BsaI GGTCTCn'nnnn_ Cuts at: 335 523 1204 4587 335 Size: 188 681 3383 631 Fragments arranged by size: 3383 681 631 188 BsaAI yAC'GTr Cuts at: 569 818 2236 569 Size: 249 1418 3216 Fragments arranged by size: 3216 1418 249 BsaBI GATnn'nnATC Cuts at: 6 2397 4434 6 Size: 2391 2037 455 Fragments arranged by size: 2391 2037 455 BsaHI Gr'CG_yC Cuts at: 128 932 1724 2314 2562 3264 4760 128 Size: 804 792 590 248 702 1496 251 Fragments arranged by size: 1496 804 792 702 590 251 248 BsaWI w'CCGG_w Cuts at: 722 1077 2072 2593 3271 3545 3692 4103 722 Size: 355 995 521 678 274 147 411 1502 Fragments arranged by size: 1502 995 678 521 411 355 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 802 832 1751 1781 802 Size: 30 919 30 3904 Fragments arranged by size: 3904 919 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 767 874 1064 1724 767 Size: 107 190 660 3926 Fragments arranged by size: 3926 660 190 107 BseYI C'CCAG_C Cuts at: 534 1553 2308 3066 3643 4139 534 Size: 1019 755 758 577 496 1278 Fragments arranged by size: 1278 1019 758 755 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 454 922 1486 454 Size: 468 564 3851 Fragments arranged by size: 3851 564 468 BsiEI CG_ry'CG Cuts at: 104 164 2425 2471 3679 104 Size: 60 2261 46 1208 1308 Fragments arranged by size: 2261 1308 1208 60 46 BsiHKAI G_wGCw'C Cuts at: 722 815 1345 1550 1897 2675 2865 3657 722 Size: 93 530 205 347 778 190 792 1948 Fragments arranged by size: 1948 792 778 530 347 205 190 93 BsiWI C'GTAC_G Cuts at: 1190 1190 Size: 4883 BsmI GAATG_Cn' Cuts at: 250 250 Size: 4883 Bsp1286I G_dGCh'C Cuts at: 722 815 874 961 1020 1146 1345 1550 1897 Size: 93 59 87 59 126 199 205 347 Cuts at: 1897 2508 2601 2675 2865 2927 3657 722 Size: 611 93 74 190 62 730 1948 Fragments arranged by size: 1948 730 611 347 205 199 190 126 93 93 87 74 62 59 59 BspEI T'CCGG_A Cuts at: 722 4103 722 Size: 3381 1502 BspHI T'CATG_A Cuts at: 927 4059 927 Size: 3132 1751 BspMI ACCTGCnnnn'nnnn_ Cuts at: 401 426 1495 1622 1786 1933 2147 2449 2830 Size: 25 1069 127 164 147 214 302 381 Cuts at: 2830 401 Size: 2454 Fragments arranged by size: 2454 1069 381 302 214 164 147 127 25 BsrBI CCG'CTC Cuts at: 50 3175 3229 4713 4878 50 Size: 3125 54 1484 165 55 Fragments arranged by size: 3125 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1385 1727 2795 1385 Size: 342 1068 3473 Fragments arranged by size: 3473 1068 342 BsrFI r'CCGG_y Cuts at: 2881 3062 3271 2881 Size: 181 209 4493 Fragments arranged by size: 4493 209 181 BssSI C'ACGA_G Cuts at: 3154 3512 3154 Size: 358 4525 BstAPI GCAn_nnn'nTGC Cuts at: 2260 2260 Size: 4883 BstBI TT'CG_AA Cuts at: 3244 3244 Size: 4883 BstYI r'GATC_y Cuts at: 1 1908 2375 2398 2733 2979 3991 4077 4089 Size: 1907 467 23 335 246 1012 86 12 Cuts at: 4089 4435 1 Size: 346 449 Fragments arranged by size: 1907 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 804 107 Size: 697 4186 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1776 2833 3014 3177 1776 Size: 1057 181 163 3482 Fragments arranged by size: 3482 1057 181 163 BtsI GCAGTG_nn' Cuts at: 1591 1848 2126 1591 Size: 257 278 4348 Fragments arranged by size: 4348 278 257 DraI TTT'AAA Cuts at: 1134 1882 1134 Size: 748 4135 DrdI GACnn_nn'nnGTC Cuts at: 1039 2589 3447 1039 Size: 1550 858 2475 Fragments arranged by size: 2475 1550 858 EaeI y'GGCC_r Cuts at: 161 189 398 2468 2642 3033 3060 4405 161 Size: 28 209 2070 174 391 27 1345 639 Fragments arranged by size: 2070 1345 639 391 209 174 28 27 EagI C'GGCC_G Cuts at: 161 2468 161 Size: 2307 2576 EarI CTCTTCn'nnn_ Cuts at: 301 460 2906 3116 301 Size: 159 2446 210 2068 Fragments arranged by size: 2446 2068 210 159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3091 3401 3547 4693 3091 Size: 310 146 1146 3281 Fragments arranged by size: 3281 1146 310 146 EcoICRI GAG'CTC Cuts at: 1895 1895 Size: 4883 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 230 317 325 539 617 1276 1411 1687 1798 Size: 87 8 214 78 659 135 276 111 Cuts at: 1798 2708 3140 3887 4155 230 Size: 910 432 747 268 958 Fragments arranged by size: 958 910 747 659 432 276 268 214 135 111 87 78 8 EcoNI CCTnn'n_nnAGG Cuts at: 975 975 Size: 4883 EcoO109I rG'GnC_Cy Cuts at: 576 980 1316 1512 2022 576 Size: 404 336 196 510 3437 Fragments arranged by size: 3437 510 404 336 196 EcoRI G'AATT_C Cuts at: 1887 1887 Size: 4883 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 781 2902 781 Size: 2121 2762 FspI TGC'GCA Cuts at: 883 2664 883 Size: 1781 3102 HaeII r_GCGC'y Cuts at: 131 909 2317 2565 3587 4566 131 Size: 778 1408 248 1022 979 448 Fragments arranged by size: 1408 1022 979 778 448 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1223 1243 1255 1275 2725 2755 2757 2787 1223 Size: 20 12 20 1450 30 2 30 3319 Fragments arranged by size: 3319 1450 30 30 20 20 12 2 HincII GTy'rAC Cuts at: 1262 1922 1262 Size: 660 4223 HindIII A'AGCT_T Cuts at: 637 1531 637 Size: 894 3989 KasI G'GCGC_C Cuts at: 127 2313 2561 127 Size: 2186 248 2449 Fragments arranged by size: 2449 2186 248 KpnI G_GTAC'C Cuts at: 660 1802 1903 660 Size: 1142 101 3640 Fragments arranged by size: 3640 1142 101 MluI A'CGCG_T Cuts at: 4316 4316 Size: 4883 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3554 3738 3554 Size: 184 4699 MscI TGG'CCA Cuts at: 2644 2644 Size: 4883 MslI CAynn'nnrTG Cuts at: 373 676 1063 1714 1870 2872 2999 373 Size: 303 387 651 156 1002 127 2257 Fragments arranged by size: 2257 1002 651 387 303 156 127 MspA1I CmG'CkG Cuts at: 373 877 964 1434 1521 1557 1994 2308 2668 Size: 504 87 470 87 36 437 314 360 Cuts at: 2668 3681 3926 373 Size: 1013 245 1330 Fragments arranged by size: 1330 1013 504 470 437 360 314 245 87 87 36 NaeI GCC'GGC Cuts at: 3064 3064 Size: 4883 NarI GG'CG_CC Cuts at: 128 2314 2562 128 Size: 2186 248 2449 Fragments arranged by size: 2449 2186 248 NcoI C'CATG_G Cuts at: 107 107 Size: 4883 NgoMIV G'CCGG_C Cuts at: 3062 3062 Size: 4883 NspI r_CATG'y Cuts at: 692 1936 2436 3343 692 Size: 1244 500 907 2232 Fragments arranged by size: 2232 1244 907 500 PasI CC'CwG_GG Cuts at: 977 977 Size: 4883 PciI A'CATG_T Cuts at: 3339 3339 Size: 4883 PflMI CCAn_nnn'nTGG Cuts at: 1185 1185 Size: 4883 PfoI T'CCnGG_A Cuts at: 1106 1775 1106 Size: 669 4214 PmeI GTTT'AAAC Cuts at: 1882 1882 Size: 4883 PmlI CAC'GTG Cuts at: 569 569 Size: 4883 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4022 4054 4022 Size: 32 4851 PpuMI rG'GwC_Cy Cuts at: 576 1512 576 Size: 936 3947 PshAI GACnn'nnGTC Cuts at: 935 935 Size: 4883 PsiI TTA'TAA Cuts at: 587 587 Size: 4883 PstI C_TGCA'G Cuts at: 1439 1520 1930 2615 1439 Size: 81 410 685 3707 Fragments arranged by size: 3707 685 410 81 PvuI CG_AT'CG Cuts at: 104 104 Size: 4883 PvuII CAG'CTG Cuts at: 964 1434 1521 1557 2308 2668 964 Size: 470 87 36 751 360 3179 Fragments arranged by size: 3179 751 470 360 87 36 RsrII CG'GwC_CG Cuts at: 3078 3078 Size: 4883 SacI G_AGCT'C Cuts at: 1897 1897 Size: 4883 SalI G'TCGA_C Cuts at: 1920 1920 Size: 4883 SapI GCTCTTCn'nnn_ Cuts at: 2906 3116 2906 Size: 210 4673 SbfI CC_TGCA'GG Cuts at: 1930 1930 Size: 4883 ScaI AGT'ACT Cuts at: 4400 4400 Size: 4883 SfcI C'TryA_G Cuts at: 33 1435 1516 1926 2611 3604 3795 33 Size: 1402 81 410 685 993 191 1121 Fragments arranged by size: 1402 1121 993 685 410 191 81 SfoI GGC'GCC Cuts at: 129 2315 2563 129 Size: 2186 248 2449 Fragments arranged by size: 2449 2186 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4883 SmaI CCC'GGG Cuts at: 268 1905 268 Size: 1637 3246 SmlI C'TyrA_G Cuts at: 711 1576 2038 2953 3445 3707 711 Size: 865 462 915 492 262 1887 Fragments arranged by size: 1887 915 865 492 462 262 SpeI A'CTAG_T Cuts at: 67 1326 67 Size: 1259 3624 SphI G_CATG'C Cuts at: 692 1936 2436 692 Size: 1244 500 3139 Fragments arranged by size: 3139 1244 500 StyI C'CwwG_G Cuts at: 107 953 1420 2017 2371 107 Size: 846 467 597 354 2619 Fragments arranged by size: 2619 846 597 467 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1372 1386 2466 2599 3058 3267 1372 Size: 14 1080 133 459 209 2988 Fragments arranged by size: 2988 1080 459 209 133 14 TatI w'GTAC_w Cuts at: 1632 4398 1632 Size: 2766 2117 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4400 4432 4400 Size: 32 4851 Tth111I GACn'n_nGTC Cuts at: 2680 2680 Size: 4883 XbaI T'CTAG_A Cuts at: 1914 1914 Size: 4883 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2378 2378 Size: 4883 XhoI C'TCGA_G Cuts at: 711 711 Size: 4883 XmaI C'CCGG_G Cuts at: 266 1903 266 Size: 1637 3246 XmnI GAAnn'nnTTC Cuts at: 1283 1283 Size: 4883 ZraI GAC'GTC Cuts at: 1725 1725 Size: 4883 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AflII AhdI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI