Restriction map for pF1KB6994
 (Circular) (Six-base) MAPSORT of: pF1KB6994.seq  Check: 3817  from: 1  to: 3872



pF1KB6994  3872 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    156     156 
   Size:       3872
 
AccI GT'mk_AC

Cuts at:    910     910 
   Size:       3872
 
Acc65I G'GTAC_C

Cuts at:    888     888 
   Size:       3872
 
AclI AA'CG_TT

Cuts at:   3712    3712 
   Size:       3872
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    428    1697    2129    2876    3144     428 
   Size:       1269     432     747     268    1156

  Fragments arranged by size: 

                1269    1156     747     432     268
 
AflIII A'CryG_T

Cuts at:   1224    2328    3305    1224 
   Size:       1104     977    1791

  Fragments arranged by size: 

                1791    1104     977
 
AgeI A'CCGG_T

Cuts at:   2260    2260 
   Size:       3872
 
AlwNI CAG_nnn'CTG

Cuts at:    717    1177    2744     717 
   Size:        460    1567    1845

  Fragments arranged by size: 

                1845    1567     460
 
ApaLI G'TGCA_C

Cuts at:   2642    2642 
   Size:       3872
 
ApoI r'AATT_y

Cuts at:    388     501     720     818     876    3225    3481     388 
   Size:        113     219      98      58    2349     256     779

  Fragments arranged by size: 

                2349     779     256     219     113      98      58
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3872
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3872
 
AvaI C'yCGr_G

Cuts at:    892     892 
   Size:       3872
 
BamHI G'GATC_C

Cuts at:    651     897     651 
   Size:        246    3626
 
BanI G'GyrC_C

Cuts at:    888    1302    1550    1585     888 
   Size:        414     248      35    3175

  Fragments arranged by size: 

                3175     414     248      35
 
BanII G_rGCy'C

Cuts at:    886    1916     886 
   Size:       1030    2842
 
BbeI G_GCGC'C

Cuts at:   1306    1554    1306 
   Size:        248    3624
 
BbsI GAAGACnn'nnnn_

Cuts at:    602     602 
   Size:       3872
 
BbvCI CC'TCA_GC

Cuts at:    343     343 
   Size:       3872
 
BciVI GTATCCnnnnn_n'

Cuts at:   1770    2531    3872    1770 
   Size:        761    1341    1770

  Fragments arranged by size: 

                1770    1341     761
 
BclI T'GATC_A

Cuts at:    417    1392     417 
   Size:        975    2897
 
BglII A'GATC_T

Cuts at:    665    1387    3424     665 
   Size:        722    2037    1113

  Fragments arranged by size: 

                2037    1113     722
 
BlpI GC'TnA_GC

Cuts at:    205     984     205 
   Size:        779    3093
 
Bme1580I G_kGCm'C

Cuts at:   1497    1590    2646    1497 
   Size:         93    1056    2723

  Fragments arranged by size: 

                2723    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1084    1165    1500    3644    1084 
   Size:         81     335    2144    1312

  Fragments arranged by size: 

                2144    1312     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2239    2239 
   Size:       3872
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    281     349     531     281 
   Size:         68     182    3622

  Fragments arranged by size: 

                3622     182      68
 
Bpu10I CC'TnA_GC

Cuts at:    343     343 
   Size:       3872
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1048    1927    2419    2717    1048 
   Size:        879     492     298    2203

  Fragments arranged by size: 

                2203     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    505    3576     505 
   Size:       3071     801
 
BsaAI yAC'GTr

Cuts at:   1225    1225 
   Size:       3872
 
BsaBI GATnn'nnATC

Cuts at:      6    1386    3423       6 
   Size:       1380    2037     455

  Fragments arranged by size: 

                2037    1380     455
 
BsaHI Gr'CG_yC

Cuts at:   1303    1551    2253    3749    1303 
   Size:        248     702    1496    1426

  Fragments arranged by size: 

                1496    1426     702     248
 
BsaWI w'CCGG_w

Cuts at:    765    1061    1582    2260    2534    2681    3092     765 
   Size:        296     521     678     274     147     411    1545

  Fragments arranged by size: 

                1545     678     521     411     296     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    220     220 
   Size:       3872
 
BseYI C'CCAG_C

Cuts at:    131    1297    2055    2632    3128     131 
   Size:       1166     758     577     496     875

  Fragments arranged by size: 

                1166     875     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1414    1460    2668     104 
   Size:       1310      46    1208    1308

  Fragments arranged by size: 

                1310    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    886    1664    1854    2646     886 
   Size:        778     190     792    2112

  Fragments arranged by size: 

                2112     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    639     864     639 
   Size:        225    3647
 
Bsp1286I G_dGCh'C

Cuts at:    886    1497    1590    1664    1854    1916    2646     886 
   Size:        611      93      74     190      62     730    2112

  Fragments arranged by size: 

                2112     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3092    3092 
   Size:       3872
 
BspHI T'CATG_A

Cuts at:    420    3048     420 
   Size:       2628    1244
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    156     347     922    1136    1438    1819     156 
   Size:        191     575     214     302     381    2209

  Fragments arranged by size: 

                2209     575     381     302     214     191
 
BsrBI CCG'CTC

Cuts at:     50    2164    2218    3702    3867      50 
   Size:       2114      54    1484     165      55

  Fragments arranged by size: 

                2114    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1784    1784 
   Size:       3872
 
BsrFI r'CCGG_y

Cuts at:    200    1870    2051    2260     200 
   Size:       1670     181     209    1812

  Fragments arranged by size: 

                1812    1670     209     181
 
BssHII G'CGCG_C

Cuts at:    220     220 
   Size:       3872
 
BssSI C'ACGA_G

Cuts at:   2143    2501    2143 
   Size:        358    3514
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1249    1249 
   Size:       3872
 
BstBI TT'CG_AA

Cuts at:   2233    2233 
   Size:       3872
 
BstXI CCAn_nnnn'nTGG

Cuts at:    453     453 
   Size:       3872
 
BstYI r'GATC_y

Cuts at:      1     651     665     897    1364    1387    1722    1968    2980 
   Size:        650      14     232     467      23     335     246    1012

Cuts at:   2980    3066    3078    3424       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     650     467     449     346     335     246     232
                  86      23      14      12
 
BtgI C'CryG_G

Cuts at:    152     287     152 
   Size:        135    3737
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1822    2003    2166    1822 
   Size:        181     163    3528

  Fragments arranged by size: 

                3528     181     163
 
BtsI GCAGTG_nn'

Cuts at:    202     833    1115     202 
   Size:        631     282    2959

  Fragments arranged by size: 

                2959     631     282
 
DraI TTT'AAA

Cuts at:    871     871 
   Size:       3872
 
DrdI GACnn_nn'nnGTC

Cuts at:   1578    2436    1578 
   Size:        858    3014
 
EaeI y'GGCC_r

Cuts at:    155     272     290    1457    1631    2022    2049    3394     155 
   Size:        117      18    1167     174     391      27    1345     633

  Fragments arranged by size: 

                1345    1167     633     391     174     117      27      18
            
 
EagI C'GGCC_G

Cuts at:   1457    1457 
   Size:       3872
 
EarI CTCTTCn'nnn_

Cuts at:    404    1895    2105     404 
   Size:       1491     210    2171

  Fragments arranged by size: 

                2171    1491     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2080    2390    2536    3682    2080 
   Size:        310     146    1146    2270

  Fragments arranged by size: 

                2270    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    884     884 
   Size:       3872
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    281     349     428     531    1697    2129    2876    3144     281 
   Size:         68      79     103    1166     432     747     268    1009

  Fragments arranged by size: 

                1166    1009     747     432     268     103      79      68
            
 
EcoO109I rG'GnC_Cy

Cuts at:    714    1011     714 
   Size:        297    3575
 
EcoRI G'AATT_C

Cuts at:    818     876     818 
   Size:         58    3814
 
EcoRV GAT'ATC

Cuts at:    429     429 
   Size:       3872
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1891    1891 
   Size:       3872
 
FspI TGC'GCA

Cuts at:   1653    1653 
   Size:       3872
 
HaeII r_GCGC'y

Cuts at:   1306    1554    2576    3555    1306 
   Size:        248    1022     979    1623

  Fragments arranged by size: 

                1623    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    837     869    1714    1744    1746    1776     837 
   Size:         32     845      30       2      30    2933

  Fragments arranged by size: 

                2933     845      32      30      30       2
 
HincII GTy'rAC

Cuts at:    911     911 
   Size:       3872
 
HindIII A'AGCT_T

Cuts at:    640     640 
   Size:       3872
 
KasI G'GCGC_C

Cuts at:   1302    1550    1302 
   Size:        248    3624
 
KpnI G_GTAC'C

Cuts at:    892     892 
   Size:       3872
 
MluI A'CGCG_T

Cuts at:   3305    3305 
   Size:       3872
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2543    2727    2543 
   Size:        184    3688
 
MscI TGG'CCA

Cuts at:    274     292    1633     274 
   Size:         18    1341    2513

  Fragments arranged by size: 

                2513    1341      18
 
MslI CAynn'nnrTG

Cuts at:   1861    1988    1861 
   Size:        127    3745
 
MspA1I CmG'CkG

Cuts at:    154     175     983    1297    1657    2670    2915     154 
   Size:         21     808     314     360    1013     245    1111

  Fragments arranged by size: 

                1111    1013     808     360     314     245      21
 
NaeI GCC'GGC

Cuts at:    202    2053     202 
   Size:       1851    2021
 
NarI GG'CG_CC

Cuts at:   1303    1551    1303 
   Size:        248    3624
 
NgoMIV G'CCGG_C

Cuts at:    200    2051     200 
   Size:       1851    2021
 
NspI r_CATG'y

Cuts at:    213     363     687     925    1425    2332     213 
   Size:        150     324     238     500     907    1753

  Fragments arranged by size: 

                1753     907     500     324     238     150
 
PasI CC'CwG_GG

Cuts at:    118     118 
   Size:       3872
 
PciI A'CATG_T

Cuts at:   2328    2328 
   Size:       3872
 
PfoI T'CCnGG_A

Cuts at:    254     254 
   Size:       3872
 
PmeI GTTT'AAAC

Cuts at:    871     871 
   Size:       3872
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3011    3043    3011 
   Size:         32    3840
 
PsiI TTA'TAA

Cuts at:    776     776 
   Size:       3872
 
PstI C_TGCA'G

Cuts at:    197     919    1604     197 
   Size:        722     685    2465

  Fragments arranged by size: 

                2465     722     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3872
 
PvuII CAG'CTG

Cuts at:   1297    1657    1297 
   Size:        360    3512
 
RsrII CG'GwC_CG

Cuts at:   2067    2067 
   Size:       3872
 
SacI G_AGCT'C

Cuts at:    886     886 
   Size:       3872
 
SacII CC_GC'GG

Cuts at:    155     155 
   Size:       3872
 
SalI G'TCGA_C

Cuts at:    909     909 
   Size:       3872
 
SapI GCTCTTCn'nnn_

Cuts at:   1895    2105    1895 
   Size:        210    3662
 
SbfI CC_TGCA'GG

Cuts at:    919     919 
   Size:       3872
 
ScaI AGT'ACT

Cuts at:   3389    3389 
   Size:       3872
 
SexAI A'CCwGG_T

Cuts at:    611     611 
   Size:       3872
 
SfcI C'TryA_G

Cuts at:     33     193     915    1600    2593    2784      33 
   Size:        160     722     685     993     191    1121

  Fragments arranged by size: 

                1121     993     722     685     191     160
 
SfoI GGC'GCC

Cuts at:   1304    1552    1304 
   Size:        248    3624
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3872
 
SmaI CCC'GGG

Cuts at:    894     894 
   Size:       3872
 
SmlI C'TyrA_G

Cuts at:   1027    1942    2434    2696    1027 
   Size:        915     492     262    2203

  Fragments arranged by size: 

                2203     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3872
 
SphI G_CATG'C

Cuts at:    925    1425     925 
   Size:        500    3372
 
StuI AGG'CCT

Cuts at:    460     460 
   Size:       3872
 
StyI C'CwwG_G

Cuts at:   1006    1360    1006 
   Size:        354    3518
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1455    1588    2047    2256    1455 
   Size:        133     459     209    3071

  Fragments arranged by size: 

                3071     459     209     133
 
TatI w'GTAC_w

Cuts at:   3387    3387 
   Size:       3872
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3389    3421    3389 
   Size:         32    3840
 
Tth111I GACn'n_nGTC

Cuts at:   1669    1669 
   Size:       3872
 
XbaI T'CTAG_A

Cuts at:    384     672     903     384 
   Size:        288     231    3353

  Fragments arranged by size: 

                3353     288     231
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1367    1367 
   Size:       3872
 
XmaI C'CCGG_G

Cuts at:    892     892 
   Size:       3872
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PasI     PciI     PfoI     PmeI     PpiI
     PsiI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BcgI    BfrBI     BglI    BmgBI     BmtI
    BsaXI     BsgI    BsiWI    BsmBI    BsrGI   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI     FseI    FspAI     HpaI     MfeI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI    PflMI
     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI    SanDI     SfiI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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