Restriction map for pF1KB6992
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BpmI        BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiWI       BsmFI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        Eam1105I    EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        HincII      
HindII      HindIII     HpaI        KpnI        MaeI        
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
SspI        StuI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcyI          267  GGCGCC               G[GA]^CG[CT]C
AocI          247  CCTAAGG              CC^T[ACGT]AGG
ApaI          308  GGGCCC               GGGCC^C
Asp700I       691  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         361  GAGCAC               G[AT]GC[AT]^C
AvaI          380  CCCGGG               C^[CT]CG[GA]G
BanI          267  GGCGCC               G^G[CT][GA]CC
BbeI          267  GGCGCC               GGCGC^C
BlpI           59  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       59  GCTCAGC              GC^T[ACGT]AGC
BsaHI         267  GGCGCC               G[GA]^CG[CT]C
BsiHKAI       361  GAGCAC               G[AT]GC[AT]^C
BsmBI          47  CGTCTC               CGTCTC(1/5)
BsoBI         380  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      413  TGTACA               T^GTACA
BsrGI         413  TGTACA               T^GTACA
BstF5I        708  GGATG                GGATG(2/0)
BstNI         121  CCAGG                CC^[AT]GG
Bsu36I        247  CCTAAGG              CC^T[ACGT]AGG
CelII          59  GCTCAGC              GC^T[ACGT]AGC
DpnII         643  GATC                 ^GATC
EarI          503  GAAGAG               CTCTTC(1/4)
Eco81I        247  CCTAAGG              CC^T[ACGT]AGG
EcoRII        121  CCAGG                ^CC[AT]GG
FokI          708  GGATG                GGATG(9/13)
Hin1I         267  GGCGCC               G[GA]^CG[CT]C
KasI          267  GGCGCC               G^GCGCC
Ksp632I       503  GAAGAG               CTCTTC(1/4)
MaeII          46  ACGT                 A^CGT
MboI          643  GATC                 ^GATC
MseI          605  TTAA                 T^TAA
MvaI          121  CCAGG                CC^[AT]GG
NarI          267  GGCGCC               GG^CGCC
NdeII         643  GATC                 ^GATC
NlaIII        333  CATG                 CATG^
NspI          332  GCATGC               [GA]CATG^[CT]
PleI            3  GACTC                GAGTC(4/5)
Sau3AI        643  GATC                 ^GATC
SfaNI          66  GATGC                GCATC(5/9)
SfoI          267  GGCGCC               GGC^GCC
SmaI          380  CCCGGG               CCC^GGG
SphI          332  GCATGC               GCATG^C
SspBI         413  TGTACA               T^GTACA
TaqI          645  TCGA                 T^CGA
TfiI          156  GATTC                G^A[AT]TC
Tru9I         605  TTAA                 T^TAA
TthHB8I       645  TCGA                 T^CGA
XmaCI         380  CCCGGG               C^CCGGG
XmaI          380  CCCGGG               C^CCGGG
XmnI          691  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

Aor51HI       175  AGCGCT               AGC^GCT
              480  AGCGCT              
BanII         308  GGGCCC               G[GA]GC[CT]^C
              384  GGGCTC              
BsiEI         135  CGGCCG               CG[GA][CT]^CG
              163  CGGCCG              
BsmAI         280  GAGAC                GTCTC(1/5)
               48  GTCTC               
BssHII        391  GCGCGC               G^CGCGC
              393  GCGCGC              
EagI          135  CGGCCG               C^GGCCG
              163  CGGCCG              
EclXI         135  CGGCCG               C^GGCCG
              163  CGGCCG              
Eco47III      175  AGCGCT               AGC^GCT
              480  AGCGCT              
Eco52I        135  CGGCCG               C^GGCCG
              163  CGGCCG              
EcoT14I       192  CCAAGG               C^C[AT][AT]GG
              704  CCTTGG              
HinfI           3  GACTC                G^A[ACGT]TC
              156  GATTC               
HphI          640  GGTGA                GGTGA(8/7)
              295  TCACC               
StyI          192  CCAAGG               C^C[AT][AT]GG
              704  CCTTGG              
Tsp509I       607  AATT                 ^AATT
              648  AATT                
TspRI         417  CAGTG                CA[GC]TG[ACGT][ACGT]^
              744  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        BbvI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DsaI        EaeI        
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        KspI        
MboII       MnlI        MspA1I      MspI        MvnI        
MwoI        NciI        NlaIV       RsaI        SacII       
Sau96I      ScrFI       TseI        
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