Restriction map for pF1KB6992
 (Circular) (Six-base) MAPSORT of: pF1KB6992.seq  Check: 9288  from: 1  to: 3869



pF1KB6992  3869 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AccI GT'mk_AC

Cuts at:    907     907 
   Size:       3869
 
Acc65I G'GTAC_C

Cuts at:    885     885 
   Size:       3869
 
AclI AA'CG_TT

Cuts at:   3709    3709 
   Size:       3869
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1694    2126    2873    3141    1694 
   Size:        432     747     268    2422

  Fragments arranged by size: 

                2422     747     432     268
 
AfeI AGC'GCT

Cuts at:    285     590     285 
   Size:        305    3564
 
AflIII A'CryG_T

Cuts at:   1221    2325    3302    1221 
   Size:       1104     977    1788

  Fragments arranged by size: 

                1788    1104     977
 
AgeI A'CCGG_T

Cuts at:   2257    2257 
   Size:       3869
 
AlwNI CAG_nnn'CTG

Cuts at:   1174    2741    1174 
   Size:       1567    2302
 
ApaI G_GGCC'C

Cuts at:    420     420 
   Size:       3869
 
ApaLI G'TGCA_C

Cuts at:   2639    2639 
   Size:       3869
 
ApoI r'AATT_y

Cuts at:    873    3222    3478     873 
   Size:       2349     256    1264

  Fragments arranged by size: 

                2349    1264     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3869
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3869
 
AvaI C'yCGr_G

Cuts at:    488     889     488 
   Size:        401    3468
 
BamHI G'GATC_C

Cuts at:    894     894 
   Size:       3869
 
BanI G'GyrC_C

Cuts at:    375     885    1299    1547    1582     375 
   Size:        510     414     248      35    2662

  Fragments arranged by size: 

                2662     510     414     248      35
 
BanII G_rGCy'C

Cuts at:    420     496     883    1913     420 
   Size:         76     387    1030    2376

  Fragments arranged by size: 

                2376    1030     387      76
 
BbeI G_GCGC'C

Cuts at:    379    1303    1551     379 
   Size:        924     248    2697

  Fragments arranged by size: 

                2697     924     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   1767    2528    3869    1767 
   Size:        761    1341    1767

  Fragments arranged by size: 

                1767    1341     761
 
BclI T'GATC_A

Cuts at:   1389    1389 
   Size:       3869
 
BglII A'GATC_T

Cuts at:   1384    3421    1384 
   Size:       2037    1832
 
BlpI GC'TnA_GC

Cuts at:    168     981     168 
   Size:        813    3056
 
Bme1580I G_kGCm'C

Cuts at:    420    1494    1587    2643     420 
   Size:       1074      93    1056    1646

  Fragments arranged by size: 

                1646    1074    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    401    1081    1162    1497    3641     401 
   Size:        680      81     335    2144     629

  Fragments arranged by size: 

                2144     680     629     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2236    2236 
   Size:       3869
 
Bpu10I CC'TnA_GC

Cuts at:    295     295 
   Size:       3869
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1045    1924    2416    2714    1045 
   Size:        879     492     298    2200

  Fragments arranged by size: 

                2200     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3573    3573 
   Size:       3869
 
BsaAI yAC'GTr

Cuts at:   1222    1222 
   Size:       3869
 
BsaBI GATnn'nnATC

Cuts at:      6    1383    3420       6 
   Size:       1377    2037     455

  Fragments arranged by size: 

                2037    1377     455
 
BsaHI Gr'CG_yC

Cuts at:    376    1300    1548    2250    3746     376 
   Size:        924     248     702    1496     499

  Fragments arranged by size: 

                1496     924     702     499     248
 
BsaWI w'CCGG_w

Cuts at:   1058    1579    2257    2531    2678    3089    1058 
   Size:        521     678     274     147     411    1838

  Fragments arranged by size: 

                1838     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    653     683     653 
   Size:         30    3839
 
BseYI C'CCAG_C

Cuts at:   1294    2052    2629    3125    1294 
   Size:        758     577     496    2038

  Fragments arranged by size: 

                2038     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     246     274    1411    1457    2665     104 
   Size:        142      28    1137      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1137     142      46      28
 
BsiHKAI G_wGCw'C

Cuts at:    473     883    1661    1851    2643     473 
   Size:        410     778     190     792    1699

  Fragments arranged by size: 

                1699     792     778     410     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    161     161 
   Size:       3869
 
Bsp1286I G_dGCh'C

Cuts at:    420     473     496     883    1494    1587    1661    1851    1913 
   Size:         53      23     387     611      93      74     190      62

Cuts at:   1913    2643     420 
   Size:        730    1646

  Fragments arranged by size: 

                1646     730     611     387     190      93      74      62
                  53      23
 
BspEI T'CCGG_A

Cuts at:   3089    3089 
   Size:       3869
 
BspHI T'CATG_A

Cuts at:   3045    3045 
   Size:       3869
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    919    1133    1435    1816     919 
   Size:        214     302     381    2972

  Fragments arranged by size: 

                2972     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2161    2215    3699    3864      50 
   Size:       2111      54    1484     165      55

  Fragments arranged by size: 

                2111    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1781    1781 
   Size:       3869
 
BsrFI r'CCGG_y

Cuts at:   1867    2048    2257    1867 
   Size:        181     209    3479

  Fragments arranged by size: 

                3479     209     181
 
BsrGI T'GTAC_A

Cuts at:    521     521 
   Size:       3869
 
BssHII G'CGCG_C

Cuts at:    499     501     499 
   Size:          2    3867
 
BssSI C'ACGA_G

Cuts at:   2140    2498    2140 
   Size:        358    3511
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1246    1246 
   Size:       3869
 
BstBI TT'CG_AA

Cuts at:   2230    2230 
   Size:       3869
 
BstYI r'GATC_y

Cuts at:      1     894    1361    1384    1719    1965    2977    3063    3075 
   Size:        893     467      23     335     246    1012      86      12

Cuts at:   3075    3421       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     893     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    356     356 
   Size:       3869
 
BtgI C'CryG_G

Cuts at:    212     309     476     506     622     212 
   Size:         97     167      30     116    3459

  Fragments arranged by size: 

                3459     167     116      97      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    187    1819    2000    2163     187 
   Size:       1632     181     163    1893

  Fragments arranged by size: 

                1893    1632     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1112    1112 
   Size:       3869
 
DraI TTT'AAA

Cuts at:    868     868 
   Size:       3869
 
DrdI GACnn_nn'nnGTC

Cuts at:   1575    2433    1575 
   Size:        858    3011
 
EaeI y'GGCC_r

Cuts at:    243     271     288    1454    1628    2019    2046    3391     243 
   Size:         28      17    1166     174     391      27    1345     721

  Fragments arranged by size: 

                1345    1166     721     391     174      28      27      17
            
 
EagI C'GGCC_G

Cuts at:    243     271    1454     243 
   Size:         28    1183    2658

  Fragments arranged by size: 

                2658    1183      28
 
EarI CTCTTCn'nnn_

Cuts at:    606    1892    2102     606 
   Size:       1286     210    2373

  Fragments arranged by size: 

                2373    1286     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2077    2387    2533    3679    2077 
   Size:        310     146    1146    2267

  Fragments arranged by size: 

                2267    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    881     881 
   Size:       3869
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1694    2126    2873    3141    1694 
   Size:        432     747     268    2422

  Fragments arranged by size: 

                2422     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1008    1008 
   Size:       3869
 
EcoRI G'AATT_C

Cuts at:    873     873 
   Size:       3869
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1888    1888 
   Size:       3869
 
FspI TGC'GCA

Cuts at:   1650    1650 
   Size:       3869
 
HaeII r_GCGC'y

Cuts at:    287     379     592    1303    1551    2573    3552     287 
   Size:         92     213     711     248    1022     979     604

  Fragments arranged by size: 

                1022     979     711     604     248     213      92
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    653     685     771     803    1711    1741    1743    1773     653 
   Size:         32      86      32     908      30       2      30    2749

  Fragments arranged by size: 

                2749     908      86      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    908     908 
   Size:       3869
 
KasI G'GCGC_C

Cuts at:    375    1299    1547     375 
   Size:        924     248    2697

  Fragments arranged by size: 

                2697     924     248
 
KpnI G_GTAC'C

Cuts at:    889     889 
   Size:       3869
 
MluI A'CGCG_T

Cuts at:   3302    3302 
   Size:       3869
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2540    2724    2540 
   Size:        184    3685
 
MscI TGG'CCA

Cuts at:   1630    1630 
   Size:       3869
 
MslI CAynn'nnrTG

Cuts at:   1858    1985    1858 
   Size:        127    3742
 
MspA1I CmG'CkG

Cuts at:    214     276     311     334     508     980    1294    1654    2667 
   Size:         62      35      23     174     472     314     360    1013

Cuts at:   2667    2912     214 
   Size:        245    1171

  Fragments arranged by size: 

                1171    1013     472     360     314     245     174      62
                  35      23
 
NaeI GCC'GGC

Cuts at:   2050    2050 
   Size:       3869
 
NarI GG'CG_CC

Cuts at:    376    1300    1548     376 
   Size:        924     248    2697

  Fragments arranged by size: 

                2697     924     248
 
NgoMIV G'CCGG_C

Cuts at:   2048    2048 
   Size:       3869
 
NspI r_CATG'y

Cuts at:    444     922    1422    2329     444 
   Size:        478     500     907    1984

  Fragments arranged by size: 

                1984     907     500     478
 
PciI A'CATG_T

Cuts at:   2325    2325 
   Size:       3869
 
PmeI GTTT'AAAC

Cuts at:    868     868 
   Size:       3869
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3008    3040    3008 
   Size:         32    3837
 
PspOMI G'GGCC_C

Cuts at:    416     416 
   Size:       3869
 
PstI C_TGCA'G

Cuts at:    916    1601     916 
   Size:        685    3184
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3869
 
PvuII CAG'CTG

Cuts at:   1294    1654    1294 
   Size:        360    3509
 
RsrII CG'GwC_CG

Cuts at:   2064    2064 
   Size:       3869
 
SacI G_AGCT'C

Cuts at:    883     883 
   Size:       3869
 
SacII CC_GC'GG

Cuts at:    215     312     509     215 
   Size:         97     197    3575

  Fragments arranged by size: 

                3575     197      97
 
SalI G'TCGA_C

Cuts at:    906     906 
   Size:       3869
 
SapI GCTCTTCn'nnn_

Cuts at:   1892    2102    1892 
   Size:        210    3659
 
SbfI CC_TGCA'GG

Cuts at:    916     916 
   Size:       3869
 
ScaI AGT'ACT

Cuts at:   3386    3386 
   Size:       3869
 
SfcI C'TryA_G

Cuts at:     33     912    1597    2590    2781      33 
   Size:        879     685     993     191    1121

  Fragments arranged by size: 

                1121     993     879     685     191
 
SfoI GGC'GCC

Cuts at:    377    1301    1549     377 
   Size:        924     248    2697

  Fragments arranged by size: 

                2697     924     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3869
 
SmaI CCC'GGG

Cuts at:    490     891     490 
   Size:        401    3468
 
SmlI C'TyrA_G

Cuts at:   1024    1939    2431    2693    1024 
   Size:        915     492     262    2200

  Fragments arranged by size: 

                2200     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3869
 
SphI G_CATG'C

Cuts at:    444     922    1422     444 
   Size:        478     500    2891

  Fragments arranged by size: 

                2891     500     478
 
StyI C'CwwG_G

Cuts at:    300     812    1003    1357     300 
   Size:        512     191     354    2812

  Fragments arranged by size: 

                2812     512     354     191
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    420     590    1452    1585    2044    2253     420 
   Size:        170     862     133     459     209    2036

  Fragments arranged by size: 

                2036     862     459     209     170     133
 
TatI w'GTAC_w

Cuts at:    319     521     705    3384     319 
   Size:        202     184    2679     804

  Fragments arranged by size: 

                2679     804     202     184
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3386    3418    3386 
   Size:         32    3837
 
Tth111I GACn'n_nGTC

Cuts at:   1666    1666 
   Size:       3869
 
XbaI T'CTAG_A

Cuts at:    900     900 
   Size:       3869
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1364    1364 
   Size:       3869
 
XmaI C'CCGG_G

Cuts at:    488     889     488 
   Size:        401    3468
 
XmnI GAAnn'nnTTC

Cuts at:    803     803 
   Size:       3869
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PciI     PmeI     PpiI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     AscI
    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BpmI    BseRI     BsgI    BsiWI     BsmI   BstEII    BstXI
  BstZ17I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI    PspXI     PsrI    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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