Restriction map for pF1KB6992
 (Linear) (Six-base) MAPSORT of: KIBB6992.seq  Check: 4066  from: 1  to: 756



KIBB6992.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AfeI AGC'GCT

Cuts at:      0     177     482     756 
   Size:        177     305     274

  Fragments arranged by size: 

                 305     274     177
 
ApaI G_GGCC'C

Cuts at:      0     312     756 
   Size:        312     444
 
AvaI C'yCGr_G

Cuts at:      0     380     756 
   Size:        380     376
 
BanI G'GyrC_C

Cuts at:      0     267     756 
   Size:        267     489
 
BanII G_rGCy'C

Cuts at:      0     312     388     756 
   Size:        312      76     368

  Fragments arranged by size: 

                 368     312      76
 
BbeI G_GCGC'C

Cuts at:      0     271     756 
   Size:        271     485
 
BlpI GC'TnA_GC

Cuts at:      0      60     756 
   Size:         60     696
 
Bme1580I G_kGCm'C

Cuts at:      0     312     756 
   Size:        312     444
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     293     756 
   Size:        293     463
 
Bpu10I CC'TnA_GC

Cuts at:      0     187     756 
   Size:        187     569
 
BsaHI Gr'CG_yC

Cuts at:      0     268     756 
   Size:        268     488
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     545     575     756 
   Size:        545      30     181

  Fragments arranged by size: 

                 545     181      30
 
BsiEI CG_ry'CG

Cuts at:      0     138     166     756 
   Size:        138      28     590

  Fragments arranged by size: 

                 590     138      28
 
BsiHKAI G_wGCw'C

Cuts at:      0     365     756 
   Size:        365     391
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      53     756 
   Size:         53     703
 
Bsp1286I G_dGCh'C

Cuts at:      0     312     365     388     756 
   Size:        312      53      23     368

  Fragments arranged by size: 

                 368     312      53      23
 
BsrGI T'GTAC_A

Cuts at:      0     413     756 
   Size:        413     343
 
BssHII G'CGCG_C

Cuts at:      0     391     393     756 
   Size:        391       2     363

  Fragments arranged by size: 

                 391     363       2
 
Bsu36I CC'TnA_GG

Cuts at:      0     248     756 
   Size:        248     508
 
BtgI C'CryG_G

Cuts at:      0     104     201     368     398     514     756 
   Size:        104      97     167      30     116     242

  Fragments arranged by size: 

                 242     167     116     104      97      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      79     756 
   Size:         79     677
 
EaeI y'GGCC_r

Cuts at:      0     135     163     180     756 
   Size:        135      28      17     576

  Fragments arranged by size: 

                 576     135      28      17
 
EagI C'GGCC_G

Cuts at:      0     135     163     756 
   Size:        135      28     593

  Fragments arranged by size: 

                 593     135      28
 
EarI CTCTTCn'nnn_

Cuts at:      0     498     756 
   Size:        498     258
 
HaeII r_GCGC'y

Cuts at:      0     179     271     484     756 
   Size:        179      92     213     272

  Fragments arranged by size: 

                 272     213     179      92
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     545     577     663     695     756 
   Size:        545      32      86      32      61

  Fragments arranged by size: 

                 545      86      61      32      32
 
KasI G'GCGC_C

Cuts at:      0     267     756 
   Size:        267     489
 
MspA1I CmG'CkG

Cuts at:      0     106     168     203     226     400     756 
   Size:        106      62      35      23     174     356

  Fragments arranged by size: 

                 356     174     106      62      35      23
 
NarI GG'CG_CC

Cuts at:      0     268     756 
   Size:        268     488
 
NspI r_CATG'y

Cuts at:      0     336     756 
   Size:        336     420
 
PspOMI G'GGCC_C

Cuts at:      0     308     756 
   Size:        308     448
 
SacII CC_GC'GG

Cuts at:      0     107     204     401     756 
   Size:        107      97     197     355

  Fragments arranged by size: 

                 355     197     107      97
 
SfoI GGC'GCC

Cuts at:      0     269     756 
   Size:        269     487
 
SmaI CCC'GGG

Cuts at:      0     382     756 
   Size:        382     374
 
SphI G_CATG'C

Cuts at:      0     336     756 
   Size:        336     420
 
StyI C'CwwG_G

Cuts at:      0     192     704     756 
   Size:        192     512      52

  Fragments arranged by size: 

                 512     192      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     312     482     756 
   Size:        312     170     274

  Fragments arranged by size: 

                 312     274     170
 
TatI w'GTAC_w

Cuts at:      0     211     413     597     756 
   Size:        211     202     184     159

  Fragments arranged by size: 

                 211     202     184     159
 
XmaI C'CCGG_G

Cuts at:      0     380     756 
   Size:        380     376
 
XmnI GAAnn'nnTTC

Cuts at:      0     695     756 
   Size:        695      61
 

 Enzymes that do cut:

     AfeI     ApaI     AvaI     BanI    BanII     BbeI     BlpI Bme1580I
     BmrI   Bpu10I    BsaHI    BsaXI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BsrGI   BssHII   Bsu36I     BtgI    BtgZI     EaeI     EagI     EarI
    HaeII    Hin4I     KasI   MspA1I     NarI     NspI   PspOMI    SacII
     SfoI     SmaI     SphI     StyI    TaqII     TatI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BseRI
    BseYI     BsgI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     ZraI
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