Restriction map for pF1KB6984
 (Linear) (Six-base) MAPSORT of: KIBB6984.seq  Check: 3730  from: 1  to: 741



KIBB6984.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AccI GT'mk_AC

Cuts at:      0     683     695     741 
   Size:        683      12      46

  Fragments arranged by size: 

                 683      46      12
 
AflIII A'CryG_T

Cuts at:      0     545     741 
   Size:        545     196
 
AleI CACnn'nnGTG

Cuts at:      0     140     741 
   Size:        140     601
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     204     741 
   Size:        204     537
 
AlwNI CAG_nnn'CTG

Cuts at:      0     403     480     741 
   Size:        403      77     261

  Fragments arranged by size: 

                 403     261      77
 
ApoI r'AATT_y

Cuts at:      0      96     123     741 
   Size:         96      27     618

  Fragments arranged by size: 

                 618      96      27
 
BanI G'GyrC_C

Cuts at:      0     451     741 
   Size:        451     290
 
BanII G_rGCy'C

Cuts at:      0     448     741 
   Size:        448     293
 
BbeI G_GCGC'C

Cuts at:      0     455     741 
   Size:        455     286
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     310     741 
   Size:        310     431
 
BlpI GC'TnA_GC

Cuts at:      0     496     741 
   Size:        496     245
 
Bme1580I G_kGCm'C

Cuts at:      0     663     741 
   Size:        663      78
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     226     741 
   Size:        226     515
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     380     698     741 
   Size:        262     118     318      43

  Fragments arranged by size: 

                 318     262     118      43
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     586     741 
   Size:        586     155
 
BsaAI yAC'GTr

Cuts at:      0     346     741 
   Size:        346     395
 
BsaHI Gr'CG_yC

Cuts at:      0     452     741 
   Size:        452     289
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     378     408     741 
   Size:        378      30     333

  Fragments arranged by size: 

                 378     333      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     106     330     741 
   Size:        106     224     411

  Fragments arranged by size: 

                 411     224     106
 
BseYI C'CCAG_C

Cuts at:      0     428     741 
   Size:        428     313
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     368     741 
   Size:        368     373
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     448     741 
   Size:        227     221     293

  Fragments arranged by size: 

                 293     227     221
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     448     663     741 
   Size:        227     221     215      78

  Fragments arranged by size: 

                 227     221     215      78
 
BsrDI GCAATG_nn'

Cuts at:      0     624     741 
   Size:        624     117
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     413     741 
   Size:        413     328
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     142     741 
   Size:        142     599
 
BtsI GCAGTG_nn'

Cuts at:      0     186     437     481     741 
   Size:        186     251      44     260

  Fragments arranged by size: 

                 260     251     186      44
 
DraIII CAC_nnn'GTG

Cuts at:      0     413     741 
   Size:        413     328
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     271     741 
   Size:        271     470
 
EcoICRI GAG'CTC

Cuts at:      0     446     741 
   Size:        446     295
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     380     698     741 
   Size:        262     118     318      43

  Fragments arranged by size: 

                 318     262     118      43
 
EcoRI G'AATT_C

Cuts at:      0      96     123     741 
   Size:         96      27     618

  Fragments arranged by size: 

                 618      96      27
 
HaeII r_GCGC'y

Cuts at:      0     455     741 
   Size:        455     286
 
KasI G'GCGC_C

Cuts at:      0     451     741 
   Size:        451     290
 
MslI CAynn'nnrTG

Cuts at:      0     140     208     741 
   Size:        140      68     533

  Fragments arranged by size: 

                 533     140      68
 
MspA1I CmG'CkG

Cuts at:      0      34     741 
   Size:         34     707
 
NarI GG'CG_CC

Cuts at:      0     452     741 
   Size:        452     289
 
NspI r_CATG'y

Cuts at:      0     549     741 
   Size:        549     192
 
PciI A'CATG_T

Cuts at:      0     545     741 
   Size:        545     196
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     403     741 
   Size:        403     338
 
PfoI T'CCnGG_A

Cuts at:      0     239     741 
   Size:        239     502
 
PmlI CAC'GTG

Cuts at:      0     346     741 
   Size:        346     395
 
PstI C_TGCA'G

Cuts at:      0     476     741 
   Size:        476     265
 
PvuII CAG'CTG

Cuts at:      0      34     741 
   Size:         34     707
 
SacI G_AGCT'C

Cuts at:      0     448     741 
   Size:        448     293
 
SexAI A'CCwGG_T

Cuts at:      0     110     741 
   Size:        110     631
 
SfcI C'TryA_G

Cuts at:      0     472     741 
   Size:        472     269
 
SfoI GGC'GCC

Cuts at:      0     453     741 
   Size:        453     288
 
SmlI C'TyrA_G

Cuts at:      0     565     741 
   Size:        565     176
 
StyI C'CwwG_G

Cuts at:      0     630     689     741 
   Size:        630      59      52

  Fragments arranged by size: 

                 630      59      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     159     741 
   Size:        159     582
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      31     378     410     741 
   Size:         31     347      32     331

  Fragments arranged by size: 

                 347     331      32      31
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     142     741 
   Size:        142     599
 

 Enzymes that do cut:

     AccI   AflIII     AleI     AlfI    AlwNI     ApoI     BanI    BanII
     BbeI     BbsI     BlpI Bme1580I     BmrI     BpmI    BpuEI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrDI
   BstAPI    BstXI     BtsI   DraIII     EciI  EcoICRI  Eco57MI    EcoRI
    HaeII     KasI     MslI   MspA1I     NarI     NspI     PciI    PflMI
     PfoI     PmlI     PstI    PvuII     SacI    SexAI     SfcI     SfoI
     SmlI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaBI
    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com