Restriction map for pF1KB6980
 (Circular) (Six-base) MAPSORT of: pF1KB6980.seq  Check: 8135  from: 1  to: 3848



pF1KB6980  3848 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    474     474 
   Size:       3848
 
AccI GT'mk_AC

Cuts at:    263     886     263 
   Size:        623    3225
 
Acc65I G'GTAC_C

Cuts at:    864     864 
   Size:       3848
 
AclI AA'CG_TT

Cuts at:   3688    3688 
   Size:       3848
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    333    1673    2105    2852    3120     333 
   Size:       1340     432     747     268    1061

  Fragments arranged by size: 

                1340    1061     747     432     268
 
AflIII A'CryG_T

Cuts at:   1200    2304    3281    1200 
   Size:       1104     977    1767

  Fragments arranged by size: 

                1767    1104     977
 
AgeI A'CCGG_T

Cuts at:   2236    2236 
   Size:       3848
 
AhdI GACnn_n'nnGTC

Cuts at:    390     390 
   Size:       3848
 
AlwNI CAG_nnn'CTG

Cuts at:   1153    2720    1153 
   Size:       1567    2281
 
ApaI G_GGCC'C

Cuts at:    318     318 
   Size:       3848
 
ApaLI G'TGCA_C

Cuts at:    778    2618     778 
   Size:       1840    2008
 
ApoI r'AATT_y

Cuts at:    852    3201    3457     852 
   Size:       2349     256    1243

  Fragments arranged by size: 

                2349    1243     256
 
AscI GG'CGCG_CC

Cuts at:    159     159 
   Size:       3848
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3848
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3848
 
AvaI C'yCGr_G

Cuts at:    290     296     770     868     290 
   Size:          6     474      98    3270

  Fragments arranged by size: 

                3270     474      98       6
 
BamHI G'GATC_C

Cuts at:    873     873 
   Size:       3848
 
BanI G'GyrC_C

Cuts at:    864    1278    1526    1561     864 
   Size:        414     248      35    3151

  Fragments arranged by size: 

                3151     414     248      35
 
BanII G_rGCy'C

Cuts at:    125     289     297     303     318     862    1892     125 
   Size:        164       8       6      15     544    1030    2081

  Fragments arranged by size: 

                2081    1030     544     164      15       8       6
 
BbeI G_GCGC'C

Cuts at:   1282    1530    1282 
   Size:        248    3600
 
BbvCI CC'TCA_GC

Cuts at:    625     625 
   Size:       3848
 
BciVI GTATCCnnnnn_n'

Cuts at:    551    1746    2507    3848     551 
   Size:       1195     761    1341     551

  Fragments arranged by size: 

                1341    1195     761     551
 
BclI T'GATC_A

Cuts at:   1368    1368 
   Size:       3848
 
BglI GCCn_nnn'nGGC

Cuts at:    538     538 
   Size:       3848
 
BglII A'GATC_T

Cuts at:   1363    3400    1363 
   Size:       2037    1811
 
BlpI GC'TnA_GC

Cuts at:    588     960     588 
   Size:        372    3476
 
Bme1580I G_kGCm'C

Cuts at:    318     782    1473    1566    2622     318 
   Size:        464     691      93    1056    1544

  Fragments arranged by size: 

                1544    1056     691     464      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1060    1141    1476    3620    1060 
   Size:         81     335    2144    1288

  Fragments arranged by size: 

                2144    1288     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2215    2215 
   Size:       3848
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    139     757     139 
   Size:        618    3230
 
Bpu10I CC'TnA_GC

Cuts at:    625     625 
   Size:       3848
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    724    1024    1903    2395    2693     724 
   Size:        300     879     492     298    1879

  Fragments arranged by size: 

                1879     879     492     300     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3552    3552 
   Size:       3848
 
BsaAI yAC'GTr

Cuts at:   1201    1201 
   Size:       3848
 
BsaBI GATnn'nnATC

Cuts at:      6     472    1362    3399       6 
   Size:        466     890    2037     455

  Fragments arranged by size: 

                2037     890     466     455
 
BsaHI Gr'CG_yC

Cuts at:   1279    1527    2229    3725    1279 
   Size:        248     702    1496    1402

  Fragments arranged by size: 

                1496    1402     702     248
 
BsaWI w'CCGG_w

Cuts at:   1037    1558    2236    2510    2657    3068    1037 
   Size:        521     678     274     147     411    1817

  Fragments arranged by size: 

                1817     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    112     142     445     475     730     760     112 
   Size:         30     303      30     255      30    3200

  Fragments arranged by size: 

                3200     303     255      30      30      30
 
BseYI C'CCAG_C

Cuts at:    656     834    1273    2031    2608    3104     656 
   Size:        178     439     758     577     496    1400

  Fragments arranged by size: 

                1400     758     577     496     439     178
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    436     502     706     436 
   Size:         66     204    3578

  Fragments arranged by size: 

                3578     204      66
 
BsiEI CG_ry'CG

Cuts at:    104    1390    1436    2644     104 
   Size:       1286      46    1208    1308

  Fragments arranged by size: 

                1308    1286    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    125     589     782     862    1640    1830    2622     125 
   Size:        464     193      80     778     190     792    1351

  Fragments arranged by size: 

                1351     792     778     464     193     190      80
 
BsmI GAATG_Cn'

Cuts at:    531     531 
   Size:       3848
 
Bsp1286I G_dGCh'C

Cuts at:    125     289     297     303     318     589     782     862    1473 
   Size:        164       8       6      15     271     193      80     611

Cuts at:   1473    1566    1640    1830    1892    2622     125 
   Size:         93      74     190      62     730    1351

  Fragments arranged by size: 

                1351     730     611     271     193     190     164      93
                  80      74      62      15       8       6
 
BspEI T'CCGG_A

Cuts at:   3068    3068 
   Size:       3848
 
BspHI T'CATG_A

Cuts at:   3024    3024 
   Size:       3848
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    474     898    1112    1414    1795     474 
   Size:        424     214     302     381    2527

  Fragments arranged by size: 

                2527     424     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     741    2140    2194    3678    3843      50 
   Size:        691    1399      54    1484     165      55

  Fragments arranged by size: 

                1484    1399     691     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1760    1760 
   Size:       3848
 
BsrFI r'CCGG_y

Cuts at:   1846    2027    2236    1846 
   Size:        181     209    3458

  Fragments arranged by size: 

                3458     209     181
 
BssHII G'CGCG_C

Cuts at:    159     159 
   Size:       3848
 
BssSI C'ACGA_G

Cuts at:    274     724    2119    2477     274 
   Size:        450    1395     358    1645

  Fragments arranged by size: 

                1645    1395     450     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    813    1225     813 
   Size:        412    3436
 
BstBI TT'CG_AA

Cuts at:    428    2209     428 
   Size:       1781    2067
 
BstXI CCAn_nnnn'nTGG

Cuts at:    252     252 
   Size:       3848
 
BstYI r'GATC_y

Cuts at:      1     179     278     873    1340    1363    1698    1944    2956 
   Size:        178      99     595     467      23     335     246    1012

Cuts at:   2956    3042    3054    3400       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     595     467     449     346     335     246     178
                  99      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    684     684 
   Size:       3848
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3848
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1798    1979    2142    1798 
   Size:        181     163    3504

  Fragments arranged by size: 

                3504     181     163
 
BtsI GCAGTG_nn'

Cuts at:    548    1091     548 
   Size:        543    3305
 
DraI TTT'AAA

Cuts at:    847     847 
   Size:       3848
 
DrdI GACnn_nn'nnGTC

Cuts at:   1554    2412    1554 
   Size:        858    2990
 
EaeI y'GGCC_r

Cuts at:    373    1433    1607    1998    2025    3370     373 
   Size:       1060     174     391      27    1345     851

  Fragments arranged by size: 

                1345    1060     851     391     174      27
 
EagI C'GGCC_G

Cuts at:   1433    1433 
   Size:       3848
 
EarI CTCTTCn'nnn_

Cuts at:   1871    2081    1871 
   Size:        210    3638
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    406     634    2056    2366    2512    3658     406 
   Size:        228    1422     310     146    1146     596

  Fragments arranged by size: 

                1422    1146     596     310     228     146
 
EcoICRI GAG'CTC

Cuts at:    123     860     123 
   Size:        737    3111
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    139     333     757    1673    2105    2852    3120     139 
   Size:        194     424     916     432     747     268     867

  Fragments arranged by size: 

                 916     867     747     432     424     268     194
 
EcoO109I rG'GnC_Cy

Cuts at:    314     315     506     987     314 
   Size:          1     191     481    3175

  Fragments arranged by size: 

                3175     481     191       1
 
EcoRI G'AATT_C

Cuts at:    852     852 
   Size:       3848
 
EcoRV GAT'ATC

Cuts at:    474     474 
   Size:       3848
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1867    1867 
   Size:       3848
 
FspI TGC'GCA

Cuts at:    175    1629     175 
   Size:       1454    2394
 
HaeII r_GCGC'y

Cuts at:   1282    1530    2552    3531    1282 
   Size:        248    1022     979    1599

  Fragments arranged by size: 

                1599    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    112     144     376     408    1690    1720    1722    1752     112 
   Size:         32     232      32    1282      30       2      30    2208

  Fragments arranged by size: 

                2208    1282     232      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    887     887 
   Size:       3848
 
KasI G'GCGC_C

Cuts at:   1278    1526    1278 
   Size:        248    3600
 
KpnI G_GTAC'C

Cuts at:    868     868 
   Size:       3848
 
MluI A'CGCG_T

Cuts at:   3281    3281 
   Size:       3848
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    110     728    2519    2703     110 
   Size:        618    1791     184    1255

  Fragments arranged by size: 

                1791    1255     618     184
 
MscI TGG'CCA

Cuts at:   1609    1609 
   Size:       3848
 
MslI CAynn'nnrTG

Cuts at:    815    1837    1964     815 
   Size:       1022     127    2699

  Fragments arranged by size: 

                2699    1022     127
 
MspA1I CmG'CkG

Cuts at:    450     592     646     660     959    1273    1633    2646    2891 
   Size:        142      54      14     299     314     360    1013     245

Cuts at:   2891     450 
   Size:       1407

  Fragments arranged by size: 

                1407    1013     360     314     299     245     142      54
                  14
 
NaeI GCC'GGC

Cuts at:   2029    2029 
   Size:       3848
 
NarI GG'CG_CC

Cuts at:   1279    1527    1279 
   Size:        248    3600
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3848
 
NgoMIV G'CCGG_C

Cuts at:   2027    2027 
   Size:       3848
 
NspI r_CATG'y

Cuts at:    814     901    1401    2308     814 
   Size:         87     500     907    2354

  Fragments arranged by size: 

                2354     907     500      87
 
PciI A'CATG_T

Cuts at:   2304    2304 
   Size:       3848
 
PmeI GTTT'AAAC

Cuts at:    847     847 
   Size:       3848
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2987    3019    2987 
   Size:         32    3816
 
PpuMI rG'GwC_Cy

Cuts at:    506     506 
   Size:       3848
 
PspOMI G'GGCC_C

Cuts at:    314     314 
   Size:       3848
 
PspXI vC'TCGA_Gb

Cuts at:    296     296 
   Size:       3848
 
PstI C_TGCA'G

Cuts at:    413     895    1580     413 
   Size:        482     685    2681

  Fragments arranged by size: 

                2681     685     482
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3848
 
PvuII CAG'CTG

Cuts at:    450     592     646     660    1273    1633     450 
   Size:        142      54      14     613     360    2665

  Fragments arranged by size: 

                2665     613     360     142      54      14
 
RsrII CG'GwC_CG

Cuts at:   2043    2043 
   Size:       3848
 
SacI G_AGCT'C

Cuts at:    125     862     125 
   Size:        737    3111
 
SalI G'TCGA_C

Cuts at:    885     885 
   Size:       3848
 
SapI GCTCTTCn'nnn_

Cuts at:   1871    2081    1871 
   Size:        210    3638
 
SbfI CC_TGCA'GG

Cuts at:    895     895 
   Size:       3848
 
ScaI AGT'ACT

Cuts at:   3365    3365 
   Size:       3848
 
SfcI C'TryA_G

Cuts at:     33     409     490     891    1576    2569    2760      33 
   Size:        376      81     401     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     401     376     191      81
 
SfoI GGC'GCC

Cuts at:   1280    1528    1280 
   Size:        248    3600
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3848
 
SmaI CCC'GGG

Cuts at:    292     870     292 
   Size:        578    3270
 
SmlI C'TyrA_G

Cuts at:    296     703    1003    1918    2410    2672     296 
   Size:        407     300     915     492     262    1472

  Fragments arranged by size: 

                1472     915     492     407     300     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3848
 
SphI G_CATG'C

Cuts at:    901    1401     901 
   Size:        500    3348
 
StuI AGG'CCT

Cuts at:    823     823 
   Size:       3848
 
StyI C'CwwG_G

Cuts at:    107     245     982    1336     107 
   Size:        138     737     354    2619

  Fragments arranged by size: 

                2619     737     354     138
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    470    1431    1564    2023    2232     470 
   Size:        961     133     459     209    2086

  Fragments arranged by size: 

                2086     961     459     209     133
 
TatI w'GTAC_w

Cuts at:   3363    3363 
   Size:       3848
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    445     477    3365    3397     445 
   Size:         32    2888      32     896

  Fragments arranged by size: 

                2888     896      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1645    1645 
   Size:       3848
 
XbaI T'CTAG_A

Cuts at:    879     879 
   Size:       3848
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114    1343     114 
   Size:       1229    2619
 
XhoI C'TCGA_G

Cuts at:    296     296 
   Size:       3848
 
XmaI C'CCGG_G

Cuts at:    290     868     290 
   Size:        578    3270
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI     PmeI     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AleI     AlfI     AloI    AvrII     BaeI
     BbsI     BcgI    BfrBI    BmgBI     BmtI    BseRI    BsiWI    BsmBI
    BsrGI   BstEII  BstZ17I     ClaI    CspCI   DraIII    EcoNI     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PshAI     PsiI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XmnI     ZraI
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