Restriction map for pF1KB6970
 (Linear) (Six-base) MAPSORT of: KIBB6970.seq  Check: 6085  from: 1  to: 723



KIBB6970.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     723 
   Size:        503     220
 
AflIII A'CryG_T

Cuts at:      0     680     723 
   Size:        680      43
 
ApoI r'AATT_y

Cuts at:      0     672     723 
   Size:        672      51
 
AvaI C'yCGr_G

Cuts at:      0     500     723 
   Size:        500     223
 
BamHI G'GATC_C

Cuts at:      0     116     723 
   Size:        116     607
 
BanI G'GyrC_C

Cuts at:      0     391     526     723 
   Size:        391     135     197

  Fragments arranged by size: 

                 391     197     135
 
BanII G_rGCy'C

Cuts at:      0     182     538     723 
   Size:        182     356     185

  Fragments arranged by size: 

                 356     185     182
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     581     723 
   Size:        581     142
 
BglI GCCn_nnn'nGGC

Cuts at:      0      85     723 
   Size:         85     638
 
Bme1580I G_kGCm'C

Cuts at:      0     531     723 
   Size:        531     192
 
BmtI G_CTAG'C

Cuts at:      0     652     723 
   Size:        652      71
 
BsaBI GATnn'nnATC

Cuts at:      0     383     723 
   Size:        383     340
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      30      60     723 
   Size:         30      30     663

  Fragments arranged by size: 

                 663      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     446     634     723 
   Size:        446     188      89

  Fragments arranged by size: 

                 446     188      89
 
BseYI C'CCAG_C

Cuts at:      0     181     452     723 
   Size:        181     271     271

  Fragments arranged by size: 

                 271     271     181
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     679     723 
   Size:        679      44
 
BsiHKAI G_wGCw'C

Cuts at:      0     538     723 
   Size:        538     185
 
BsmI GAATG_Cn'

Cuts at:      0     636     723 
   Size:        636      87
 
Bsp1286I G_dGCh'C

Cuts at:      0     182     531     538     723 
   Size:        182     349       7     185

  Fragments arranged by size: 

                 349     185     182       7
 
BsrGI T'GTAC_A

Cuts at:      0     486     723 
   Size:        486     237
 
BstYI r'GATC_y

Cuts at:      0     116     384     723 
   Size:        116     268     339

  Fragments arranged by size: 

                 339     268     116
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     305     340     723 
   Size:        305      35     383

  Fragments arranged by size: 

                 383     305      35
 
EaeI y'GGCC_r

Cuts at:      0     666     723 
   Size:        666      57
 
EarI CTCTTCn'nnn_

Cuts at:      0     148     698     723 
   Size:        148     550      25

  Fragments arranged by size: 

                 550     148      25
 
EcoICRI GAG'CTC

Cuts at:      0     536     723 
   Size:        536     187
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     723 
   Size:        503     220
 
EcoO109I rG'GnC_Cy

Cuts at:      0     448     479     723 
   Size:        448      31     244

  Fragments arranged by size: 

                 448     244      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     198     230     723 
   Size:        198      32     493

  Fragments arranged by size: 

                 493     198      32
 
HindIII A'AGCT_T

Cuts at:      0      94     723 
   Size:         94     629
 
MfeI C'AATT_G

Cuts at:      0     506     723 
   Size:        506     217
 
MscI TGG'CCA

Cuts at:      0     668     723 
   Size:        668      55
 
MspA1I CmG'CkG

Cuts at:      0     456     723 
   Size:        456     267
 
NdeI CA'TA_TG

Cuts at:      0     464     723 
   Size:        464     259
 
NheI G'CTAG_C

Cuts at:      0     648     723 
   Size:        648      75
 
NspI r_CATG'y

Cuts at:      0     684     723 
   Size:        684      39
 
PciI A'CATG_T

Cuts at:      0     680     723 
   Size:        680      43
 
PpuMI rG'GwC_Cy

Cuts at:      0     448     479     723 
   Size:        448      31     244

  Fragments arranged by size: 

                 448     244      31
 
PspXI vC'TCGA_Gb

Cuts at:      0     500     723 
   Size:        500     223
 
PvuII CAG'CTG

Cuts at:      0     456     723 
   Size:        456     267
 
SacI G_AGCT'C

Cuts at:      0     538     723 
   Size:        538     185
 
SfcI C'TryA_G

Cuts at:      0     104     723 
   Size:        104     619
 
SmlI C'TyrA_G

Cuts at:      0     500     723 
   Size:        500     223
 
TatI w'GTAC_w

Cuts at:      0     486     723 
   Size:        486     237
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     584     616     723 
   Size:        584      32     107

  Fragments arranged by size: 

                 584     107      32
 
XhoI C'TCGA_G

Cuts at:      0     500     723 
   Size:        500     223
 
XmnI GAAnn'nnTTC

Cuts at:      0     162     723 
   Size:        162     561
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AvaI    BamHI     BanI    BanII     BbsI
     BglI Bme1580I     BmtI    BsaBI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BsrGI    BstYI    CspCI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I    Hin4I  HindIII     MfeI     MscI   MspA1I
     NdeI     NheI     NspI     PciI    PpuMI    PspXI    PvuII     SacI
     SfcI     SmlI     TatI     TstI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EciI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MluI     MmeI     MslI     NaeI
     NarI     NcoI   NgoMIV     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XmaI     ZraI
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