Restriction map for pF1KB6965
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BanII       BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BspDI       BspEI       BsrBI       BsrDI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DdeI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     KspI        
MaeI        MamI        MfeI        MflI        MluI        
MroI        MseI        MspA1I      MunI        MvnI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          507  GTCTAC               GT^[AC][GT]AC
AcyI          500  GGCGCC               G[GA]^CG[CT]C
AflIII        470  ACATGT               A^C[GA][CT]GT
AlwI          204  GATCC                GGATC(4/5)
AlwNI         118  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         439  GTGCTC               G[AT]GC[AT]^C
AvaI          191  CCCGGG               C^[CT]CG[GA]G
BanI          500  GGCGCC               G^G[CT][GA]CC
BbeI          500  GGCGCC               GGCGC^C
BglI          187  GCCACCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          439  GTGCTC               G[AGT]GC[ACT]^C
BpmI          359  CTGGAG               CTGGAG(16/14)
BsaHI         500  GGCGCC               G[GA]^CG[CT]C
BsiHKAI       439  GTGCTC               G[AT]GC[AT]^C
BsoBI         191  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      439  GTGCTC               G[AGT]GC[ACT]^C
Bsp1407I        2  TGTACA               T^GTACA
BspLU11I      470  ACATGT               A^CATGT
BspMI         155  ACCTGC               ACCTGC(4/8)
BsrGI           2  TGTACA               T^GTACA
BstAPI        143  GCAACAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI          47  CCATCCTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII         204  GATC                 ^GATC
DsaI           65  CCATGG               C^C[GA][CT]GG
Hin1I         500  GGCGCC               G[GA]^CG[CT]C
HinfI         362  GAGTC                G^A[ACGT]TC
HphI          365  TCACC                GGTGA(8/7)
KasI          500  GGCGCC               G^GCGCC
MaeII         448  ACGT                 A^CGT
MaeIII        364  GTCAC                ^GT[ACGT]AC
MboI          204  GATC                 ^GATC
MboII         452  TCTTC                GAAGA(8/7)
MslI          234  CATCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          497  GCCGGC               GCC^GGC
NarI          500  GGCGCC               GG^CGCC
NcoI           65  CCATGG               C^CATGG
NdeII         204  GATC                 ^GATC
NgoMIV        497  GCCGGC               G^CCGGC
NspI          470  ACATGT               [GA]CATG^[CT]
PleI          362  GAGTC                GAGTC(4/5)
PpuMI         103  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          157  CTGCAG               CTGCA^G
Sau3AI        204  GATC                 ^GATC
SfcI          157  CTGCAG               C^T[GA][CT]AG
SfoI          500  GGCGCC               GGC^GCC
SmaI          191  CCCGGG               CCC^GGG
SspBI           2  TGTACA               T^GTACA
Tsp45I        364  GTCAC                ^GT[GC]AC
XcmI           47  CCATCCTCCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         191  CCCGGG               C^CCGGG
XmaI          191  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AluI            7  AGCT                 AG^CT
              373  AGCT                
BalI           62  TGGCCA               TGG^CCA
              347  TGGCCA              
BsmFI          43  GGGAC                GGGAC(10/14)
              395  GGGAC               
BsmI          183  GAATGC               GAATGC(1/-1)
              493  GAATGC              
BspHI         200  TCATGA               T^CATGA
              443  TCATGA              
BsrFI          92  ACCGGC               [GA]^CCGG[CT]
              497  GCCGGC              
Cac8I         301  GCTGGC               GC[ACGT]^[ACGT]GC
              497  GCCGGC              
Cfr10I         92  ACCGGC               [GA]^CCGG[CT]
              497  GCCGGC              
DraII         103  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              119  AGGGCCT             
EaeI           62  TGGCCA               [CT]^GGCC[GA]
              347  TGGCCA              
EcoO109I      103  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              119  AGGGCCT             
EcoT14I        65  CCATGG               C^C[AT][AT]GG
              344  CCTTGG              
FauI          278  CCCGC                CCCGC(4/6)
              515  CCCGC               
Fnu4HI        383  GCCGC                GC^[ACGT]GC
              386  GCCGC               
HaeII         401  GGCGCT               [GA]GCGC^[CT]
              500  GGCGCC              
ItaI          383  GCCGC                GC^[ACGT]GC
              386  GCCGC               
MluNI          62  TGGCCA               TGG^CCA
              347  TGGCCA              
MnlI           51  CCTC                 CCTC(7/6)
              320  CCTC                
MscI           62  TGGCCA               TGG^CCA
              347  TGGCCA              
RcaI          200  TCATGA               T^CATGA
              443  TCATGA              
StyI           65  CCATGG               C^C[AT][AT]GG
              344  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AciI        AfaI        AvaII       BcnI        BsaJI       
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstNI       CfoI        Cfr13I      EcoRII      FokI        
HaeIII      HapII       HhaI        HinP1I      HpaII       
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       SfaNI       
TspRI       
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