Restriction map for pF1KB6950
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbvCI       BciVI       BclI        BcnI        BfrI        
BglI        BlnI        BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstXI       BstZ17I     Bsu36I      Cac8I       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FokI        FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HincII      HindII      
HpaI        HpaII       HphI        KasI        KpnI        
Ksp632I     KspI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MspI        MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        StyI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII          10  CGCG                 CG^CG
Alw44I        259  GTGCAC               G^TGCAC
AlwI          448  GATCC                GGATC(4/5)
ApaI          237  GGGCCC               GGGCC^C
ApaLI         259  GTGCAC               G^TGCAC
BbsI          510  GAAGAC               GAAGAC(2/6)
BcgI          261  GCACAGAGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          277  CTAG                 C^TAG
BglII         540  AGATCT               A^GATCT
BlpI          322  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      322  GCTCAGC              GC^T[ACGT]AGC
BpuAI         510  GAAGAC               GAAGAC(2/6)
BsaJI         104  CCGTGG               C^C[ACGT][ACGT]GG
BseRI          92  GAGGAG               GAGGAG(10/8)
BsmFI         423  GGGAC                GGGAC(10/14)
BssKI         470  CCTGG                ^CC[ACGT]GG
BstNI         470  CCTGG                CC^[AT]GG
BstUI          10  CGCG                 CG^CG
CelII         322  GCTCAGC              GC^T[ACGT]AGC
CfoI            9  GCGC                 GCG^C
DraII         237  GGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI          104  CCGTGG               C^C[GA][CT]GG
EcoO109I      237  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRII        470  CCTGG                ^CC[AT]GG
FauI           11  GCGGG                CCCGC(4/6)
HaeIII        238  GGCC                 GG^CC
HhaI            9  GCGC                 GCG^C
HinP1I          9  GCGC                 G^CGC
HindIII       591  AAGCTT               A^AGCTT
HinfI         632  GAATC                G^A[ACGT]TC
MaeI          277  CTAG                 C^TAG
MspA1I        325  CAGCTG               C[AC]G^C[GT]G
MvaI          470  CCTGG                CC^[AT]GG
MvnI           10  CGCG                 CG^CG
NlaIII        234  CATG                 CATG^
PvuII         325  CAGCTG               CAG^CTG
SacI          266  GAGCTC               GAGCT^C
ScrFI         470  CCTGG                CC^[ACGT]GG
SmlI          520  CTTGAG               C^T[CT][GA]AG
TaqI          270  TCGA                 T^CGA
TfiI          632  GAATC                G^A[AT]TC
TspRI         228  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       270  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AciI            4  GCGG                 CCGC(-3/-1)
               11  GCGG                
Asp700I       307  GAACCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              510  GAAGACTTTC          
AspHI         259  GTGCAC               G[AT]GC[AT]^C
              266  GAGCTC              
BpmI           89  CTGGAG               CTGGAG(16/14)
              455  CTGGAG              
BsiHKAI       259  GTGCAC               G[AT]GC[AT]^C
              266  GAGCTC              
BsiYI          99  CCCGTCCGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              100  CCGTCCGTGGG         
BslI           99  CCCGTCCGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              100  CCGTCCGTGGG         
Bsp1407I      183  TGTACA               T^GTACA
              224  TGTACA              
BsrDI         484  GCAATG               GCAATG(2/0)
              638  GCAATG              
BsrGI         183  TGTACA               T^GTACA
              224  TGTACA              
BstYI         447  AGATCC               [GA]^GATC[CT]
              540  AGATCT              
Cfr13I        237  GGGCC                G^G[ACGT]CC
              238  GGCCC               
DpnII         448  GATC                 ^GATC
              541  GATC                
MboI          448  GATC                 ^GATC
              541  GATC                
MflI          447  AGATCC               [GA]^GATC[CT]
              540  AGATCT              
NdeII         448  GATC                 ^GATC
              541  GATC                
Sau3AI        448  GATC                 ^GATC
              541  GATC                
Sau96I        237  GGGCC                G^G[ACGT]CC
              238  GGCCC               
SfaNI         351  GATGC                GCATC(5/9)
              556  GATGC               
SspBI         183  TGTACA               T^GTACA
              224  TGTACA              
Tsp509I       111  AATT                 ^AATT
              201  AATT                
XhoII         447  AGATCC               [GA]^GATC[CT]
              540  AGATCT              
XmnI          307  GAACCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              510  GAAGACTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AfaI        AluI        BanII       BbvI        BmyI        
Bsp1286I    DdeI        Fnu4HI      ItaI        MboII       
MnlI        MseI        MwoI        NlaIV       RsaI        
SfcI        Tru9I       TseI        
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