Restriction map for pF1KB6949
 (Linear) (Six-base) MAPSORT of: KIBB6949.seq  Check: 1106  from: 1  to: 633



KIBB6949.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

Acc65I G'GTAC_C

Cuts at:      0     581     633 
   Size:        581      52
 
AlwNI CAG_nnn'CTG

Cuts at:      0      24     514     633 
   Size:         24     490     119

  Fragments arranged by size: 

                 490     119      24
 
ApaLI G'TGCA_C

Cuts at:      0     309     536     587     633 
   Size:        309     227      51      46

  Fragments arranged by size: 

                 309     227      51      46
 
AvaI C'yCGr_G

Cuts at:      0     239     633 
   Size:        239     394
 
BanI G'GyrC_C

Cuts at:      0     581     633 
   Size:        581      52
 
BanII G_rGCy'C

Cuts at:      0     100     246     633 
   Size:        100     146     387

  Fragments arranged by size: 

                 387     146     100
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     220     254     633 
   Size:        220      34     379

  Fragments arranged by size: 

                 379     220      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     232     633 
   Size:        232     401
 
Bme1580I G_kGCm'C

Cuts at:      0     313     540     591     633 
   Size:        313     227      51      42

  Fragments arranged by size: 

                 313     227      51      42
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     261     633 
   Size:        261     372
 
BseYI C'CCAG_C

Cuts at:      0      24      45      50     267     503     577     633 
   Size:         24      21       5     217     236      74      56

  Fragments arranged by size: 

                 236     217      74      56      24      21       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     633 
   Size:         54     579
 
BsiHKAI G_wGCw'C

Cuts at:      0     100     175     212     313     540     591     633 
   Size:        100      75      37     101     227      51      42

  Fragments arranged by size: 

                 227     101     100      75      51      42      37
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     320     633 
   Size:        320     313
 
Bsp1286I G_dGCh'C

Cuts at:      0     100     175     212     246     313     540     591     633 
   Size:        100      75      37      34      67     227      51      42

  Fragments arranged by size: 

                 227     100      75      67      51      42      37      34
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     376     633 
   Size:        376     257
 
BsrBI CCG'CTC

Cuts at:      0     596     633 
   Size:        596      37
 
BsrGI T'GTAC_A

Cuts at:      0     137     633 
   Size:        137     496
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     308     391     514     633 
   Size:        308      83     123     119

  Fragments arranged by size: 

                 308     123     119      83
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     288     633 
   Size:        288     345
 
BtgI C'CryG_G

Cuts at:      0      35     221     356     633 
   Size:         35     186     135     277

  Fragments arranged by size: 

                 277     186     135      35
 
BtsI GCAGTG_nn'

Cuts at:      0      91     538     633 
   Size:         91     447      95

  Fragments arranged by size: 

                 447      95      91
 
EaeI y'GGCC_r

Cuts at:      0       2     278     290     420     633 
   Size:          2     276      12     130     213

  Fragments arranged by size: 

                 276     213     130      12       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     621     633 
   Size:        621      12
 
EcoICRI GAG'CTC

Cuts at:      0      98     633 
   Size:         98     535
 
KpnI G_GTAC'C

Cuts at:      0     585     633 
   Size:        585      48
 
MscI TGG'CCA

Cuts at:      0       4     280     292     422     633 
   Size:          4     276      12     130     211

  Fragments arranged by size: 

                 276     211     130      12       4
 
MslI CAynn'nnrTG

Cuts at:      0     178     286     633 
   Size:        178     108     347

  Fragments arranged by size: 

                 347     178     108
 
MspA1I CmG'CkG

Cuts at:      0     223     633 
   Size:        223     410
 
NcoI C'CATG_G

Cuts at:      0      35     356     633 
   Size:         35     321     277

  Fragments arranged by size: 

                 321     277      35
 
PsiI TTA'TAA

Cuts at:      0     446     633 
   Size:        446     187
 
PspXI vC'TCGA_Gb

Cuts at:      0     239     633 
   Size:        239     394
 
PstI C_TGCA'G

Cuts at:      0      20     633 
   Size:         20     613
 
SacI G_AGCT'C

Cuts at:      0     100     633 
   Size:        100     533
 
SacII CC_GC'GG

Cuts at:      0     224     633 
   Size:        224     409
 
SfcI C'TryA_G

Cuts at:      0      16     633 
   Size:         16     617
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     232     633 
   Size:        232     401
 
SmlI C'TyrA_G

Cuts at:      0     239     633 
   Size:        239     394
 
StyI C'CwwG_G

Cuts at:      0      35     356     398     620     633 
   Size:         35     321      42     222      13

  Fragments arranged by size: 

                 321     222      42      35      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      42     349     633 
   Size:         42     307     284

  Fragments arranged by size: 

                 307     284      42
 
TatI w'GTAC_w

Cuts at:      0     137     633 
   Size:        137     496
 
Tth111I GACn'n_nGTC

Cuts at:      0     160     633 
   Size:        160     473
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      42     633 
   Size:         42     591
 
XhoI C'TCGA_G

Cuts at:      0     239     633 
   Size:        239     394
 

 Enzymes that do cut:

   Acc65I    AlwNI    ApaLI     AvaI     BanI    BanII     BcgI     BglI
 Bme1580I     BsaI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrGI   BstAPI    BstXI     BtgI     BtsI     EaeI     EarI
  EcoICRI     KpnI     MscI     MslI   MspA1I     NcoI     PsiI    PspXI
     PstI     SacI    SacII     SfcI     SfiI     SmlI     StyI    TaqII
     TatI  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrDI
    BsrFI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI     PsrI     PvuI
    PvuII    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI     XbaI     XmaI     XmnI     ZraI
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