Restriction map for pF1KB6942
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BseAI       BsgI        
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrGI       BsrI        
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DsaI        Eam1105I    
EarI        Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MspA1I      
MunI        NarI        NcoI        NdeI        NheI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        Tru9I       
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII         162  CGCG                 CG^CG
AcyI          672  GACGCC               G[GA]^CG[CT]C
AlwI          464  GGATC                GGATC(4/5)
AlwNI         494  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          171  GGGCCC               GGGCC^C
AspHI         652  GTGCTC               G[AT]GC[AT]^C
BanII         171  GGGCCC               G[GA]GC[CT]^C
BpmI          406  CTGGAG               CTGGAG(16/14)
BsaAI         514  TACGTG               [CT]AC^GT[GA]
BsaHI         672  GACGCC               G[GA]^CG[CT]C
BsaI          556  GAGACC               GGTCTC(1/5)
BsaWI         461  ACCGGA               [AT]^CCGG[AT]
BsiEI          71  CGGCCG               CG[GA][CT]^CG
BsiHKAI       652  GTGCTC               G[AT]GC[AT]^C
BsmAI         556  GAGAC                GTCTC(1/5)
BsmI          478  GCATTC               GAATGC(1/-1)
BspMI         138  ACCTGC               ACCTGC(4/8)
BsrBI          36  GAGCGG               CCGCTC(-3/-3)
BsrDI         267  CATTGC               GCAATG(2/0)
BsrFI         142  GCCGGC               [GA]^CCGG[CT]
BssSI         177  CACGAG               CACGAG(-5/-1)
BstUI         162  CGCG                 CG^CG
Cfr10I        142  GCCGGC               [GA]^CCGG[CT]
DraIII        567  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          672  GACGCCACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI           71  CGGCCG               [CT]^GGCC[GA]
EagI           71  CGGCCG               C^GGCCG
EclXI          71  CGGCCG               C^GGCCG
Eco52I         71  CGGCCG               C^GGCCG
EcoRV         502  GATATC               GAT^ATC
EcoT14I       548  CCAAGG               C^C[AT][AT]GG
HaeII         422  AGCGCC               [GA]GCGC^[CT]
HgaI          672  GACGC                GACGC(5/10)
Hin1I         672  GACGCC               G[GA]^CG[CT]C
HinfI         544  GAATC                G^A[ACGT]TC
MaeII         515  ACGT                 A^CGT
MvnI          162  CGCG                 CG^CG
NaeI          142  GCCGGC               GCC^GGC
NgoMIV        142  GCCGGC               G^CCGGC
NotI           70  GCGGCCGC             GC^GGCCGC
PshAI         207  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
StyI          548  CCAAGG               C^C[AT][AT]GG
TfiI          544  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI          347  AGCT                 AG^CT
              590  AGCT                
BanI           40  GGTGCC               G^G[CT][GA]CC
              135  GGCACC              
BglI           43  GCCCCGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
               76  GCCTTCCTGGC         
BstAPI        262  GCAGCCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              617  GCAAGCAGTGC         
DpnII         453  GATC                 ^GATC
              465  GATC                
MaeIII        367  GTCAC                ^GT[ACGT]AC
              596  GTGAC               
MboI          453  GATC                 ^GATC
              465  GATC                
MboII         444  GAAGA                GAAGA(8/7)
               20  TCTTC               
NdeII         453  GATC                 ^GATC
              465  GATC                
PpuMI          62  AGGACCC              [GA]G^G[AT]CC[CT]
              401  AGGACCT             
Sau3AI        453  GATC                 ^GATC
              465  GATC                
Tsp45I        367  GTCAC                ^GT[GC]AC
              596  GTGAC               
TspRI         622  CAGTG                CA[GC]TG[ACGT][ACGT]^
              677  CACTG               
XcmI          395  CCAAGAAGGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              562  CCAGGCACAGAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AvaII       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MnlI        MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       Sau96I      ScrFI       SfaNI       
TseI        
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