Restriction map for pF1KB6942
 (Circular) (Six-base) MAPSORT of: pF1KB6942.seq  Check: 1598  from: 1  to: 3800



pF1KB6942  3800 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    255     255 
   Size:       3800
 
AccI GT'mk_AC

Cuts at:    838     838 
   Size:       3800
 
Acc65I G'GTAC_C

Cuts at:    816     816 
   Size:       3800
 
AclI AA'CG_TT

Cuts at:   3640    3640 
   Size:       3800
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    793    1625    2057    2804    3072     793 
   Size:        832     432     747     268    1521

  Fragments arranged by size: 

                1521     832     747     432     268
 
AflIII A'CryG_T

Cuts at:   1152    2256    3233    1152 
   Size:       1104     977    1719

  Fragments arranged by size: 

                1719    1104     977
 
AgeI A'CCGG_T

Cuts at:   2188    2188 
   Size:       3800
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    620     620 
   Size:       3800
 
AlwNI CAG_nnn'CTG

Cuts at:    607    1105    2672     607 
   Size:        498    1567    1735

  Fragments arranged by size: 

                1735    1567     498
 
ApaI G_GGCC'C

Cuts at:    283     283 
   Size:       3800
 
ApaLI G'TGCA_C

Cuts at:   2570    2570 
   Size:       3800
 
ApoI r'AATT_y

Cuts at:    804    3153    3409     804 
   Size:       2349     256    1195

  Fragments arranged by size: 

                2349    1195     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3800
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3800
 
AvaI C'yCGr_G

Cuts at:    820     820 
   Size:       3800
 
BamHI G'GATC_C

Cuts at:    825     825 
   Size:       3800
 
BanI G'GyrC_C

Cuts at:    148     243     816    1230    1478    1513     148 
   Size:         95     573     414     248      35    2435

  Fragments arranged by size: 

                2435     573     414     248      95      35
 
BanII G_rGCy'C

Cuts at:    283     814    1844     283 
   Size:        531    1030    2239

  Fragments arranged by size: 

                2239    1030     531
 
BbeI G_GCGC'C

Cuts at:   1234    1482    1234 
   Size:        248    3552
 
BciVI GTATCCnnnnn_n'

Cuts at:   1698    2459    3800    1698 
   Size:        761    1341    1698

  Fragments arranged by size: 

                1698    1341     761
 
BclI T'GATC_A

Cuts at:   1320    1320 
   Size:       3800
 
BglI GCCn_nnn'nGGC

Cuts at:    157     190     157 
   Size:         33    3767
 
BglII A'GATC_T

Cuts at:   1315    3352    1315 
   Size:       2037    1763
 
BlpI GC'TnA_GC

Cuts at:    912     912 
   Size:       3800
 
Bme1580I G_kGCm'C

Cuts at:    153     246     283    1425    1518    2574     153 
   Size:         93      37    1142      93    1056    1379

  Fragments arranged by size: 

                1379    1142    1056      93      93      37
 
BmrI ACTGGGnnnn_n'

Cuts at:   1012    1093    1428    3572    1012 
   Size:         81     335    2144    1240

  Fragments arranged by size: 

                2144    1240     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2167    2167 
   Size:       3800
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    535     535 
   Size:       3800
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    976    1855    2347    2645     976 
   Size:        879     492     298    2131

  Fragments arranged by size: 

                2131     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    658    3504     658 
   Size:       2846     954
 
BsaAI yAC'GTr

Cuts at:    624    1153     624 
   Size:        529    3271
 
BsaBI GATnn'nnATC

Cuts at:      6    1314    3351       6 
   Size:       1308    2037     455

  Fragments arranged by size: 

                2037    1308     455
 
BsaHI Gr'CG_yC

Cuts at:    781    1231    1479    2181    3677     781 
   Size:        450     248     702    1496     904

  Fragments arranged by size: 

                1496     904     702     450     248
 
BsaWI w'CCGG_w

Cuts at:    569     989    1510    2188    2462    2609    3020     569 
   Size:        420     521     678     274     147     411    1349

  Fragments arranged by size: 

                1349     678     521     420     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    181     445     532     649     676     181 
   Size:        264      87     117      27    3305

  Fragments arranged by size: 

                3305     264     117      87      27
 
BseYI C'CCAG_C

Cuts at:    194     695    1225    1983    2560    3056     194 
   Size:        501     530     758     577     496     938

  Fragments arranged by size: 

                 938     758     577     530     501     496
 
BsiEI CG_ry'CG

Cuts at:    104     182    1342    1388    2596     104 
   Size:         78    1160      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1160      78      46
 
BsiHKAI G_wGCw'C

Cuts at:    764     814    1592    1782    2574     764 
   Size:         50     778     190     792    1990

  Fragments arranged by size: 

                1990     792     778     190      50
 
BsmI GAATG_Cn'

Cuts at:    586     586 
   Size:       3800
 
Bsp1286I G_dGCh'C

Cuts at:    153     246     283     764     814    1425    1518    1592    1782 
   Size:         93      37     481      50     611      93      74     190

Cuts at:   1782    1844    2574     153 
   Size:         62     730    1379

  Fragments arranged by size: 

                1379     730     611     481     190      93      93      74
                  62      50      37
 
BspEI T'CCGG_A

Cuts at:   3020    3020 
   Size:       3800
 
BspHI T'CATG_A

Cuts at:   2976    2976 
   Size:       3800
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    255     850    1064    1366    1747     255 
   Size:        595     214     302     381    2308

  Fragments arranged by size: 

                2308     595     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     146    2092    2146    3630    3795      50 
   Size:         96    1946      54    1484     165      55

  Fragments arranged by size: 

                1946    1484     165      96      55      54
 
BsrDI GCAATG_nn'

Cuts at:    374    1712     374 
   Size:       1338    2462
 
BsrFI r'CCGG_y

Cuts at:    250    1798    1979    2188     250 
   Size:       1548     181     209    1862

  Fragments arranged by size: 

                1862    1548     209     181
 
BssSI C'ACGA_G

Cuts at:    285    2071    2429     285 
   Size:       1786     358    1656

  Fragments arranged by size: 

                1786    1656     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    376     731    1177     376 
   Size:        355     446    2999

  Fragments arranged by size: 

                2999     446     355
 
BstBI TT'CG_AA

Cuts at:   2161    2161 
   Size:       3800
 
BstYI r'GATC_y

Cuts at:      1     825    1292    1315    1650    1896    2908    2994    3006 
   Size:        824     467      23     335     246    1012      86      12

Cuts at:   3006    3352       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     824     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3800
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1750    1931    2094    1750 
   Size:        181     163    3456

  Fragments arranged by size: 

                3456     181     163
 
BtsI GCAGTG_nn'

Cuts at:    736    1043     736 
   Size:        307    3493
 
DraI TTT'AAA

Cuts at:    799     799 
   Size:       3800
 
DraIII CAC_nnn'GTG

Cuts at:    680     680 
   Size:       3800
 
DrdI GACnn_nn'nnGTC

Cuts at:    786    1506    2364     786 
   Size:        720     858    2222

  Fragments arranged by size: 

                2222     858     720
 
EaeI y'GGCC_r

Cuts at:    179    1385    1559    1950    1977    3322     179 
   Size:       1206     174     391      27    1345     657

  Fragments arranged by size: 

                1345    1206     657     391     174      27
 
EagI C'GGCC_G

Cuts at:    179    1385     179 
   Size:       1206    2594
 
EarI CTCTTCn'nnn_

Cuts at:   1823    2033    1823 
   Size:        210    3590
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    178    2008    2318    2464    3610     178 
   Size:       1830     310     146    1146     368

  Fragments arranged by size: 

                1830    1146     368     310     146
 
EcoICRI GAG'CTC

Cuts at:    812     812 
   Size:       3800
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    535     793    1625    2057    2804    3072     535 
   Size:        258     832     432     747     268    1263

  Fragments arranged by size: 

                1263     832     747     432     268     258
 
EcoO109I rG'GnC_Cy

Cuts at:    171     280     510     939     171 
   Size:        109     230     429    3032

  Fragments arranged by size: 

                3032     429     230     109
 
EcoRI G'AATT_C

Cuts at:    804     804 
   Size:       3800
 
EcoRV GAT'ATC

Cuts at:    612     612 
   Size:       3800
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1819    1819 
   Size:       3800
 
FspI TGC'GCA

Cuts at:   1581    1581 
   Size:       3800
 
HaeII r_GCGC'y

Cuts at:    534    1234    1482    2504    3483     534 
   Size:        700     248    1022     979     851

  Fragments arranged by size: 

                1022     979     851     700     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    451     483    1642    1672    1674    1704     451 
   Size:         32    1159      30       2      30    2547

  Fragments arranged by size: 

                2547    1159      32      30      30       2
 
HincII GTy'rAC

Cuts at:    839     839 
   Size:       3800
 
KasI G'GCGC_C

Cuts at:   1230    1478    1230 
   Size:        248    3552
 
KpnI G_GTAC'C

Cuts at:    820     820 
   Size:       3800
 
MluI A'CGCG_T

Cuts at:   3233    3233 
   Size:       3800
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    119    2471    2655     119 
   Size:       2352     184    1264

  Fragments arranged by size: 

                2352    1264     184
 
MscI TGG'CCA

Cuts at:   1561    1561 
   Size:       3800
 
MslI CAynn'nnrTG

Cuts at:   1789    1916    1789 
   Size:        127    3673
 
MspA1I CmG'CkG

Cuts at:    911    1225    1585    2598    2843     911 
   Size:        314     360    1013     245    1868

  Fragments arranged by size: 

                1868    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:    252    1981     252 
   Size:       1729    2071
 
NarI GG'CG_CC

Cuts at:   1231    1479    1231 
   Size:        248    3552
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3800
 
NgoMIV G'CCGG_C

Cuts at:    250    1979     250 
   Size:       1729    2071
 
NotI GC'GGCC_GC

Cuts at:    179     179 
   Size:       3800
 
NspI r_CATG'y

Cuts at:    853    1353    2260     853 
   Size:        500     907    2393

  Fragments arranged by size: 

                2393     907     500
 
PciI A'CATG_T

Cuts at:   2256    2256 
   Size:       3800
 
PmeI GTTT'AAAC

Cuts at:    799     799 
   Size:       3800
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2939    2971    2939 
   Size:         32    3768
 
PpuMI rG'GwC_Cy

Cuts at:    171     510     171 
   Size:        339    3461
 
PshAI GACnn'nnGTC

Cuts at:    319     319 
   Size:       3800
 
PspOMI G'GGCC_C

Cuts at:    279     279 
   Size:       3800
 
PstI C_TGCA'G

Cuts at:    847    1532     847 
   Size:        685    3115
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3800
 
PvuII CAG'CTG

Cuts at:   1225    1585    1225 
   Size:        360    3440
 
RsrII CG'GwC_CG

Cuts at:   1995    1995 
   Size:       3800
 
SacI G_AGCT'C

Cuts at:    814     814 
   Size:       3800
 
SalI G'TCGA_C

Cuts at:    837     837 
   Size:       3800
 
SapI GCTCTTCn'nnn_

Cuts at:   1823    2033    1823 
   Size:        210    3590
 
SbfI CC_TGCA'GG

Cuts at:    847     847 
   Size:       3800
 
ScaI AGT'ACT

Cuts at:   3317    3317 
   Size:       3800
 
SfcI C'TryA_G

Cuts at:     33     843    1528    2521    2712      33 
   Size:        810     685     993     191    1121

  Fragments arranged by size: 

                1121     993     810     685     191
 
SfoI GGC'GCC

Cuts at:   1232    1480    1232 
   Size:        248    3552
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3800
 
SmaI CCC'GGG

Cuts at:    822     822 
   Size:       3800
 
SmlI C'TyrA_G

Cuts at:    955    1870    2362    2624     955 
   Size:        915     492     262    2131

  Fragments arranged by size: 

                2131     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3800
 
SphI G_CATG'C

Cuts at:    853    1353     853 
   Size:        500    3300
 
StyI C'CwwG_G

Cuts at:    107     656     934    1288     107 
   Size:        549     278     354    2619

  Fragments arranged by size: 

                2619     549     354     278
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1383    1516    1975    2184    1383 
   Size:        133     459     209    2999

  Fragments arranged by size: 

                2999     459     209     133
 
TatI w'GTAC_w

Cuts at:   3315    3315 
   Size:       3800
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3317    3349    3317 
   Size:         32    3768
 
Tth111I GACn'n_nGTC

Cuts at:   1597    1597 
   Size:       3800
 
XbaI T'CTAG_A

Cuts at:    831     831 
   Size:       3800
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    510     677    1295     510 
   Size:        167     618    3015

  Fragments arranged by size: 

                3015     618     167
 
XmaI C'CCGG_G

Cuts at:    820     820 
   Size:       3800
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NotI     NspI     PciI     PmeI     PpiI    PpuMI    PshAI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AloI     AscI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI     BmtI   Bpu10I
    BsaXI     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI    EcoNI     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmlI     PsiI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     XmnI     ZraI
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