Restriction map for pF1KB6942
 (Linear) (Six-base) MAPSORT of: KIBB6942.seq  Check: 1066  from: 1  to: 687



KIBB6942.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     147     687 
   Size:        147     540
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685     687 
   Size:        685       2
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     512     687 
   Size:        512     175
 
AlwNI CAG_nnn'CTG

Cuts at:      0     499     687 
   Size:        499     188
 
ApaI G_GGCC'C

Cuts at:      0     175     687 
   Size:        175     512
 
BanI G'GyrC_C

Cuts at:      0      40     135     687 
   Size:         40      95     552

  Fragments arranged by size: 

                 552      95      40
 
BanII G_rGCy'C

Cuts at:      0     175     687 
   Size:        175     512
 
BglI GCCn_nnn'nGGC

Cuts at:      0      49      82     687 
   Size:         49      33     605

  Fragments arranged by size: 

                 605      49      33
 
Bme1580I G_kGCm'C

Cuts at:      0      45     138     175     687 
   Size:         45      93      37     512

  Fragments arranged by size: 

                 512      93      45      37
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     687 
   Size:        427     260
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     550     687 
   Size:        550     137
 
BsaAI yAC'GTr

Cuts at:      0     516     687 
   Size:        516     171
 
BsaHI Gr'CG_yC

Cuts at:      0     673     687 
   Size:        673      14
 
BsaWI w'CCGG_w

Cuts at:      0     461     687 
   Size:        461     226
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     337     424     541     568     687 
   Size:         73     264      87     117      27     119

  Fragments arranged by size: 

                 264     119     117      87      73      27
 
BseYI C'CCAG_C

Cuts at:      0      86     587     687 
   Size:         86     501     100

  Fragments arranged by size: 

                 501     100      86
 
BsiEI CG_ry'CG

Cuts at:      0      74     687 
   Size:         74     613
 
BsiHKAI G_wGCw'C

Cuts at:      0     656     687 
   Size:        656      31
 
BsmI GAATG_Cn'

Cuts at:      0     478     687 
   Size:        478     209
 
Bsp1286I G_dGCh'C

Cuts at:      0      45     138     175     656     687 
   Size:         45      93      37     481      31

  Fragments arranged by size: 

                 481      93      45      37      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     147     687 
   Size:        147     540
 
BsrBI CCG'CTC

Cuts at:      0      38     687 
   Size:         38     649
 
BsrDI GCAATG_nn'

Cuts at:      0     266     687 
   Size:        266     421
 
BsrFI r'CCGG_y

Cuts at:      0     142     687 
   Size:        142     545
 
BssSI C'ACGA_G

Cuts at:      0     177     687 
   Size:        177     510
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     268     623     687 
   Size:        268     355      64

  Fragments arranged by size: 

                 355     268      64
 
BtsI GCAGTG_nn'

Cuts at:      0     628     687 
   Size:        628      59
 
DraIII CAC_nnn'GTG

Cuts at:      0     572     687 
   Size:        572     115
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     678     687 
   Size:        678       9
 
EaeI y'GGCC_r

Cuts at:      0      71     687 
   Size:         71     616
 
EagI C'GGCC_G

Cuts at:      0      71     687 
   Size:         71     616
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      70     687 
   Size:         70     617
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     685     687 
   Size:        427     258       2

  Fragments arranged by size: 

                 427     258       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0      63     172     402     687 
   Size:         63     109     230     285

  Fragments arranged by size: 

                 285     230     109      63
 
EcoRV GAT'ATC

Cuts at:      0     504     687 
   Size:        504     183
 
HaeII r_GCGC'y

Cuts at:      0     426     687 
   Size:        426     261
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     343     375     687 
   Size:        343      32     312

  Fragments arranged by size: 

                 343     312      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      11     687 
   Size:         11     676
 
NaeI GCC'GGC

Cuts at:      0     144     687 
   Size:        144     543
 
NgoMIV G'CCGG_C

Cuts at:      0     142     687 
   Size:        142     545
 
NotI GC'GGCC_GC

Cuts at:      0      71     687 
   Size:         71     616
 
PpuMI rG'GwC_Cy

Cuts at:      0      63     402     687 
   Size:         63     339     285

  Fragments arranged by size: 

                 339     285      63
 
PshAI GACnn'nnGTC

Cuts at:      0     211     687 
   Size:        211     476
 
PspOMI G'GGCC_C

Cuts at:      0     171     687 
   Size:        171     516
 
StyI C'CwwG_G

Cuts at:      0     548     687 
   Size:        548     139
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     402     569     687 
   Size:        402     167     118

  Fragments arranged by size: 

                 402     167     118
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     ApaI     BanI    BanII     BglI
 Bme1580I     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI     BtsI   DraIII     DrdI     EaeI     EagI     EciI
  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I     MmeI     NaeI   NgoMIV
     NotI    PpuMI    PshAI   PspOMI     StyI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaBI    BsaXI     BsgI    BsiWI    BsmBI
    BspEI    BspHI    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     EarI
  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NheI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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