Restriction map for pF1KB6935
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

Acc65I      AccI        AclI        AfaI        AflII       
AflIII      AhdI        AlwI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsiEI       BsiWI       BsmAI       BsmBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DpnII       
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MaeIII      MamI        MboI        MfeI        
MflI        MluI        MluNI       MscI        MseI        
MslI        MunI        NarI        NciI        NcoI        
NdeI        NdeII       NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacII       SalI        Sau3AI      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TfiI        Tru9I       Tsp45I      Tth111I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         111  GACGTC               GACGT^C
AcsI          319  AAATTC               [GA]^AATT[CT]
AcyI          111  GACGTC               G[GA]^CG[CT]C
AgeI          521  ACCGGT               A^CCGGT
Alw44I        183  GTGCAC               G^TGCAC
AlwNI          76  CAGGGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         183  GTGCAC               G^TGCAC
ApoI          319  AAATTC               [GA]^AATT[CT]
Asp700I       496  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BpmI           55  CTGGAG               CTGGAG(16/14)
BsaHI         111  GACGTC               G[GA]^CG[CT]C
BseRI         392  GAGGAG               GAGGAG(10/8)
BsgI           82  CTGCAC               GTGCAG(16/14)
BsmI          198  GAATGC               GAATGC(1/-1)
BsrBI         581  GAGCGG               CCGCTC(-3/-3)
BsrDI         397  GCAATG               GCAATG(2/0)
CfoI          379  GCGC                 GCG^C
DdeI          552  CTCAG                C^T[ACGT]AG
DraIII        436  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI          99  CCTGGGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI          379  GCGC                 GCG^C
Hin1I         111  GACGTC               G[GA]^CG[CT]C
HinP1I        379  GCGC                 G^CGC
HinfI         274  GAGTC                G^A[ACGT]TC
MaeII         112  ACGT                 A^CGT
NaeI          243  GCCGGC               GCC^GGC
NgoMIV        243  GCCGGC               G^CCGGC
NlaIII        511  CATG                 CATG^
PinAI         521  ACCGGT               A^CCGGT
PleI          274  GAGTC                GAGTC(4/5)
PpuMI         134  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         119  CAGCTG               CAG^CTG
SacI          549  GAGCTC               GAGCT^C
SapI          247  GCTCTTC              GCTCTTC(1/4)
SmlI          574  CTCAAG               C^T[CT][GA]AG
TaqI          227  TCGA                 T^CGA
TthHB8I       227  TCGA                 T^CGA
XmnI          496  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII         378  CGCG                 CG^CG
              380  CGCG                
AccIII        165  TCCGGA               T^CCGGA
              475  TCCGGA              
AspHI         183  GTGCAC               G[AT]GC[AT]^C
              549  GAGCTC              
AvaI          217  CCCGAG               C^[CT]CG[GA]G
              594  CTCGGG              
AvaII         135  GGTCC                G^G[AT]CC
              163  GGTCC               
BseAI         165  TCCGGA               T^CCGGA
              475  TCCGGA              
BsiHKAI       183  GTGCAC               G[AT]GC[AT]^C
              549  GAGCTC              
BsoBI         217  CCCGAG               C^[CT]CG[GA]G
              594  CTCGGG              
BspEI         165  TCCGGA               T^CCGGA
              475  TCCGGA              
BsrFI         243  GCCGGC               [GA]^CCGG[CT]
              521  ACCGGT              
BsrI           33  ACTGG                ACTGG(1/-1)
              483  ACTGG               
BstAPI         75  GCAGGGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              118  GCAGCTGATGC         
BstUI         378  CGCG                 CG^CG
              380  CGCG                
Cfr10I        243  GCCGGC               [GA]^CCGG[CT]
              521  ACCGGT              
DraII         134  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              565  GGGGCCT             
DsaI          128  CCGTGG               C^C[GA][CT]GG
              461  CCGTGG              
EaeI          485  TGGCCG               [CT]^GGCC[GA]
              612  TGGCCG              
EarI          248  CTCTTC               CTCTTC(1/4)
              229  GAAGAG              
EcoO109I      134  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              565  GGGGCCT             
HphI          252  TCACC                GGTGA(8/7)
              281  TCACC               
Ksp632I       248  CTCTTC               CTCTTC(1/4)
              229  GAAGAG              
MboII         229  GAAGA                GAAGA(8/7)
              249  TCTTC               
MroI          165  TCCGGA               T^CCGGA
              475  TCCGGA              
MvnI          378  CGCG                 CG^CG
              380  CGCG                
PflMI         435  CCACAGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              519  CCACCGGTTGG         
PstI          116  CTGCAG               CTGCA^G
              447  CTGCAG              
SfaNI         124  GATGC                GCATC(5/9)
              177  GATGC               
SfcI          116  CTGCAG               C^T[GA][CT]AG
              447  CTGCAG              
Tsp509I       320  AATT                 ^AATT
              347  AATT                
Van91I        435  CCACAGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              519  CCACCGGTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BmyI        
BsaJI       BsaWI       BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HpaII       ItaI        MnlI        
MspA1I      MspI        MvaI        MwoI        NlaIV       
Sau96I      ScrFI       TseI        TspRI       
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