Restriction map for pF1KB6924
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvrII       BamHI       BbrPI       BbsI        BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BseAI       BsgI        BsiHKAI     BsiWI       BsmAI       
BsmBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstF5I      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FokI        FseI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     MamI        MboII       
MfeI        MflI        MluI        MroI        MseI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

ApaI          298  GGGCCC               GGGCC^C
AvaI          219  CCCGGG               C^[CT]CG[GA]G
AviII         629  TGCGCA               TGC^GCA
BalI          356  TGGCCA               TGG^CCA
BanII         298  GGGCCC               G[GA]GC[CT]^C
BbeI          422  GGCGCC               GGCGC^C
BbvCI          55  CCTCAGC              CCTCAGC(-5/-2)
BcgI          501  GCAGATGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         237  GGATAC               GTATCC(6/5)
BfaI          148  CTAG                 C^TAG
BsmI           76  GCATTC               GAATGC(1/-1)
BsoBI         219  CCCGGG               C^[CT]CG[GA]G
BspHI         539  TCATGA               T^CATGA
BstAPI        270  GCAGGACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI           56  CTCAG                C^T[ACGT]AG
DraII         493  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI          356  TGGCCA               [CT]^GGCC[GA]
EcoNI         519  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      493  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I        10  CCAAGG               C^C[AT][AT]GG
FspI          629  TGCGCA               TGC^GCA
HaeII         422  GGCGCC               [GA]GCGC^[CT]
HphI          264  GGTGA                GGTGA(8/7)
KasI          422  GGCGCC               G^GCGCC
KspI          157  CCGCGG               CCGC^GG
MaeI          148  CTAG                 C^TAG
MaeII         548  ACGT                 A^CGT
MluNI         356  TGGCCA               TGG^CCA
MscI          356  TGGCCA               TGG^CCA
NarI          422  GGCGCC               GG^CGCC
PpuMI         493  AGGACCT              [GA]G^G[AT]CC[CT]
PstI           61  CTGCAG               CTGCA^G
PvuII          58  CAGCTG               CAG^CTG
RcaI          539  TCATGA               T^CATGA
SacII         157  CCGCGG               CCGC^GG
SfoI          422  GGCGCC               GGC^GCC
SmaI          219  CCCGGG               CCC^GGG
SmlI          168  CTCAAG               C^T[CT][GA]AG
SphI          350  GCATGC               GCATG^C
StyI           10  CCAAGG               C^C[AT][AT]GG
XcmI          182  CCACACTCATCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         219  CCCGGG               C^CCGGG
XmaI          219  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI          255  GGCGTC               G[GA]^CG[CT]C
              422  GGCGCC              
AfaI          621  GTAC                 GT^AC
              675  GTAC                
BanI          422  GGCGCC               G^G[CT][GA]CC
              597  GGTGCC              
BpmI          390  CTGGAG               CTGGAG(16/14)
              520  CTGGAG              
BsaHI         255  GGCGTC               G[GA]^CG[CT]C
              422  GGCGCC              
BsaWI         225  ACCGGA               [AT]^CCGG[AT]
              488  ACCGGA              
BsiEI         115  CGGTCG               CG[GA][CT]^CG
              486  CGACCG              
BsmFI         140  GGGAC                GGGAC(10/14)
              222  GGGAC               
BsrI          389  ACTGG                ACTGG(1/-1)
              557  ACTGG               
BstNI          86  CCAGG                CC^[AT]GG
              519  CCTGG               
DpnII         378  GATC                 ^GATC
              609  GATC                
DsaI          157  CCGCGG               C^C[GA][CT]GG
              660  CCACGG              
EcoRII         86  CCAGG                ^CC[AT]GG
              519  CCTGG               
FauI          109  CCCGC                CCCGC(4/6)
              590  CCCGC               
HgaI          256  GCGTC                GACGC(5/10)
              327  GCGTC               
Hin1I         255  GGCGTC               G[GA]^CG[CT]C
              422  GGCGCC              
HinfI          17  GAGTC                G^A[ACGT]TC
              571  GAGTC               
MboI          378  GATC                 ^GATC
              609  GATC                
MslI          192  CATGGCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              534  CATCATCATG          
MvaI           86  CCAGG                CC^[AT]GG
              519  CCTGG               
NdeII         378  GATC                 ^GATC
              609  GATC                
NspI          275  ACATGC               [GA]CATG^[CT]
              350  GCATGC              
PleI           17  GAGTC                GAGTC(4/5)
              571  GAGTC               
RsaI          621  GTAC                 GT^AC
              675  GTAC                
Sau3AI        378  GATC                 ^GATC
              609  GATC                
SfaNI         504  GATGC                GCATC(5/9)
              452  GCATC               
SfcI           61  CTGCAG               C^T[GA][CT]AG
              345  CTACAG              
Tsp45I         19  GTCAC                ^GT[GC]AC
              209  GTCAC               
TspRI         388  CACTG                CA[GC]TG[ACGT][ACGT]^
              467  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BssKI       BstUI       Cac8I       
CfoI        Cfr13I      Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MaeIII      
MnlI        MspA1I      MspI        MvnI        MwoI        
NciI        NlaIII      NlaIV       Sau96I      ScrFI       
TaqI        TseI        TthHB8I     
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