Restriction map for
pF1KB6924
(Linear) (Six-base) MAPSORT of: KIBB6924.seq Check: 2183 from: 1 to: 681
KIBB6924.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 562 594 681
Size: 562 32 87
Fragments arranged by size:
562 87 32
ApaI G_GGCC'C
Cuts at: 0 302 681
Size: 302 379
AvaI C'yCGr_G
Cuts at: 0 219 681
Size: 219 462
BanI G'GyrC_C
Cuts at: 0 422 597 681
Size: 422 175 84
Fragments arranged by size:
422 175 84
BanII G_rGCy'C
Cuts at: 0 302 681
Size: 302 379
BbeI G_GCGC'C
Cuts at: 0 426 681
Size: 426 255
BbvCI CC'TCA_GC
Cuts at: 0 56 681
Size: 56 625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 490 524 681
Size: 490 34 157
Fragments arranged by size:
490 157 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 231 681
Size: 231 450
Bme1580I G_kGCm'C
Cuts at: 0 302 389 554 602 681
Size: 302 87 165 48 79
Fragments arranged by size:
302 165 87 79 48
BmgBI CAC'GTC
Cuts at: 0 549 681
Size: 549 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 541 681
Size: 411 130 140
Fragments arranged by size:
411 140 130
Bpu10I CC'TnA_GC
Cuts at: 0 56 681
Size: 56 625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 681
Size: 153 528
BsaHI Gr'CG_yC
Cuts at: 0 256 423 681
Size: 256 167 258
Fragments arranged by size:
258 256 167
BsaWI w'CCGG_w
Cuts at: 0 225 488 681
Size: 225 263 193
Fragments arranged by size:
263 225 193
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 562 592 681
Size: 562 30 89
Fragments arranged by size:
562 89 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 56 538 541 655 681
Size: 56 482 3 114 26
Fragments arranged by size:
482 114 56 26 3
BseYI C'CCAG_C
Cuts at: 0 593 681
Size: 593 88
BsiEI CG_ry'CG
Cuts at: 0 118 489 681
Size: 118 371 192
Fragments arranged by size:
371 192 118
BsmI GAATG_Cn'
Cuts at: 0 76 681
Size: 76 605
Bsp1286I G_dGCh'C
Cuts at: 0 302 389 554 602 681
Size: 302 87 165 48 79
Fragments arranged by size:
302 165 87 79 48
BspHI T'CATG_A
Cuts at: 0 539 681
Size: 539 142
BstAPI GCAn_nnn'nTGC
Cuts at: 0 276 681
Size: 276 405
BtgI C'CryG_G
Cuts at: 0 157 660 681
Size: 157 503 21
Fragments arranged by size:
503 157 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 438 681
Size: 438 243
EaeI y'GGCC_r
Cuts at: 0 356 681
Size: 356 325
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 82 168 205 272 681
Size: 82 86 37 67 409
Fragments arranged by size:
409 86 82 67 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 541 681
Size: 411 130 140
Fragments arranged by size:
411 140 130
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 681
Size: 523 158
EcoO109I rG'GnC_Cy
Cuts at: 0 494 681
Size: 494 187
FspI TGC'GCA
Cuts at: 0 631 681
Size: 631 50
HaeII r_GCGC'y
Cuts at: 0 426 681
Size: 426 255
KasI G'GCGC_C
Cuts at: 0 422 681
Size: 422 259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 681
Size: 98 583
MscI TGG'CCA
Cuts at: 0 358 681
Size: 358 323
MslI CAynn'nnrTG
Cuts at: 0 196 538 681
Size: 196 342 143
Fragments arranged by size:
342 196 143
MspA1I CmG'CkG
Cuts at: 0 60 159 593 681
Size: 60 99 434 88
Fragments arranged by size:
434 99 88 60
NarI GG'CG_CC
Cuts at: 0 423 681
Size: 423 258
NspI r_CATG'y
Cuts at: 0 279 354 681
Size: 279 75 327
Fragments arranged by size:
327 279 75
PasI CC'CwG_GG
Cuts at: 0 86 681
Size: 86 595
PfoI T'CCnGG_A
Cuts at: 0 137 681
Size: 137 544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 562 594 681
Size: 562 32 87
Fragments arranged by size:
562 87 32
PpuMI rG'GwC_Cy
Cuts at: 0 494 681
Size: 494 187
PspOMI G'GGCC_C
Cuts at: 0 298 681
Size: 298 383
PstI C_TGCA'G
Cuts at: 0 65 681
Size: 65 616
PvuII CAG'CTG
Cuts at: 0 60 681
Size: 60 621
SacII CC_GC'GG
Cuts at: 0 160 681
Size: 160 521
SfcI C'TryA_G
Cuts at: 0 61 345 681
Size: 61 284 336
Fragments arranged by size:
336 284 61
SfoI GGC'GCC
Cuts at: 0 424 681
Size: 424 257
SmaI CCC'GGG
Cuts at: 0 221 681
Size: 221 460
SmlI C'TyrA_G
Cuts at: 0 168 681
Size: 168 513
SphI G_CATG'C
Cuts at: 0 354 681
Size: 354 327
StyI C'CwwG_G
Cuts at: 0 10 681
Size: 10 671
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 585 681
Size: 585 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 215 247 681
Size: 215 32 434
Fragments arranged by size:
434 215 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 681
Size: 189 492
XmaI C'CCGG_G
Cuts at: 0 219 681
Size: 219 462
Enzymes that do cut:
AloI ApaI AvaI BanI BanII BbeI BbvCI BcgI
BciVI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsmI Bsp1286I BspHI BstAPI
BtgI BtgZI EaeI EciI Eco57MI EcoNI EcoO109I FspI
HaeII KasI MmeI MscI MslI MspA1I NarI NspI
PasI PfoI PpiI PpuMI PspOMI PstI PvuII SacII
SfcI SfoI SmaI SmlI SphI StyI TaqII TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BclI
BfrBI BglI BglII BlpI BmrI BmtI BplI BsaI
BsaAI BsaBI BsgI BsiHKAI BsiWI BsmBI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV FalI
FseI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI
XmnI ZraI