Restriction map for pF1KB6923
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwI        AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XcmI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII          40  CGCG                 CG^CG
AcsI          599  AAATTC               [GA]^AATT[CT]
AfaI          487  GTAC                 GT^AC
Alw44I        341  GTGCAC               G^TGCAC
AlwNI         244  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         341  GTGCAC               G^TGCAC
ApoI          599  AAATTC               [GA]^AATT[CT]
BanII         178  GAGCTC               G[GA]GC[CT]^C
BclI          392  TGATCA               T^GATCA
BsaJI          17  CCCGGG               C^C[ACGT][ACGT]GG
BsgI          136  GTGCAG               GTGCAG(16/14)
BsmAI         357  GTCTC                GTCTC(1/5)
BsmFI         510  GGGAC                GGGAC(10/14)
BsrI          249  ACTGG                ACTGG(1/-1)
BstF5I        375  GGATG                GGATG(2/0)
BstUI          40  CGCG                 CG^CG
DraII         284  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EaeI           59  TGGCCG               [CT]^GGCC[GA]
EarI          502  GAAGAG               CTCTTC(1/4)
EcoO109I      284  AGGTCCT              [GA]G^G[ACGT]CC[CT]
FauI           41  GCGGG                CCCGC(4/6)
FbaI          392  TGATCA               T^GATCA
FokI          375  GGATG                GGATG(9/13)
HapII          18  CCGG                 C^CGG
HpaII          18  CCGG                 C^CGG
Ksp632I       502  GAAGAG               CTCTTC(1/4)
MspI           18  CCGG                 C^CGG
MvnI           40  CGCG                 CG^CG
MwoI           80  GCATCAACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        585  CATG                 CATG^
NlaIV         510  GGGACC               GG[ACGT]^[ACGT]CC
PaeR7I        131  CTCGAG               C^TCGAG
PpuMI         284  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI            7  CTGCAG               CTGCA^G
RsaI          487  GTAC                 GT^AC
SacI          178  GAGCTC               GAGCT^C
SfaNI          80  GCATC                GCATC(5/9)
SfcI            7  CTGCAG               C^T[GA][CT]AG
SmaI           17  CCCGGG               CCC^GGG
SspI          268  AATATT               AAT^ATT
TaqI          132  TCGA                 T^CGA
TfiI           29  GAATC                G^A[AT]TC
TthHB8I       132  TCGA                 T^CGA
XhoI          131  CTCGAG               C^TCGAG
XmaCI          17  CCCGGG               C^CCGGG
XmaI           17  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AciI          543  CCGC                 CCGC(-3/-1)
               41  GCGG                
AspHI         178  GAGCTC               G[AT]GC[AT]^C
              341  GTGCAC              
AvaI           17  CCCGGG               C^[CT]CG[GA]G
              131  CTCGAG              
AvaII         285  GGTCC                G^G[AT]CC
              511  GGACC               
BbvI            9  GCAGC                GCAGC(8/12)
                6  GCTGC               
BcnI           17  CCCGG                CC^[GC]GG
               18  CCGGG               
BmyI          178  GAGCTC               G[AGT]GC[ACT]^C
              341  GTGCAC              
BsiHKAI       178  GAGCTC               G[AT]GC[AT]^C
              341  GTGCAC              
BsiYI          18  CCGGGAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               35  CCCTGCGCGGG         
BslI           18  CCGGGAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               35  CCCTGCGCGGG         
BsoBI          17  CCCGGG               C^[CT]CG[GA]G
              131  CTCGAG              
Bsp1286I      178  GAGCTC               G[AGT]GC[ACT]^C
              341  GTGCAC              
BstNI         288  CCTGG                CC^[AT]GG
              372  CCAGG               
Cfr13I        285  GGTCC                G^G[ACGT]CC
              511  GGACC               
DdeI          359  CTCAG                C^T[ACGT]AG
              533  CTGAG               
EcoRII        288  CCTGG                ^CC[AT]GG
              372  CCAGG               
Fnu4HI          6  GCTGC                GC^[ACGT]GC
                9  GCAGC               
HaeII           3  GGCGCT               [GA]GCGC^[CT]
               46  AGCGCC              
HaeIII         60  GGCC                 GG^CC
              591  GGCC                
ItaI            6  GCTGC                GC^[ACGT]GC
                9  GCAGC               
MboII         502  GAAGA                GAAGA(8/7)
               68  TCTTC               
MnlI          283  GAGG                 CCTC(7/6)
              535  GAGG                
MvaI          288  CCTGG                CC^[AT]GG
              372  CCAGG               
NciI           17  CCCGG                CC^[GC]GG
               18  CCGGG               
PleI          149  GACTC                GAGTC(4/5)
              505  GAGTC               
Sau96I        285  GGTCC                G^G[ACGT]CC
              511  GGACC               
SmlI          131  CTCGAG               C^T[CT][GA]AG
              175  CTTGAG              
TseI            6  GCTGC                G^C[AT]GC
                9  GCAGC               
Tsp45I        317  GTGAC                ^GT[GC]AC
              415  GTGAC               
Tsp509I       529  AATT                 ^AATT
              600  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BssKI       CfoI        DpnII       HhaI        
HinP1I      HinfI       HphI        MaeIII      MboI        
NdeII       Sau3AI      ScrFI       TspRI       
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