Restriction map for pF1KB6923
 (Circular) (Six-base) MAPSORT of: pF1KB6923.seq  Check: 7077  from: 1  to: 3728



pF1KB6923  3728 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:    766     766 
   Size:       3728
 
Acc65I G'GTAC_C

Cuts at:    744     744 
   Size:       3728
 
AclI AA'CG_TT

Cuts at:   3568    3568 
   Size:       3728
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1553    1985    2732    3000    1553 
   Size:        432     747     268    2281

  Fragments arranged by size: 

                2281     747     432     268
 
AflIII A'CryG_T

Cuts at:   1080    2184    3161    1080 
   Size:       1104     977    1647

  Fragments arranged by size: 

                1647    1104     977
 
AgeI A'CCGG_T

Cuts at:   2116    2116 
   Size:       3728
 
AlwNI CAG_nnn'CTG

Cuts at:    357    1033    2600     357 
   Size:        676    1567    1485

  Fragments arranged by size: 

                1567    1485     676
 
ApaLI G'TGCA_C

Cuts at:    449    2498     449 
   Size:       2049    1679
 
ApoI r'AATT_y

Cuts at:    707     732    3081    3337     707 
   Size:         25    2349     256    1098

  Fragments arranged by size: 

                2349    1098     256      25
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3728
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3728
 
AvaI C'yCGr_G

Cuts at:    125     239     748     125 
   Size:        114     509    3105

  Fragments arranged by size: 

                3105     509     114
 
BamHI G'GATC_C

Cuts at:    753     753 
   Size:       3728
 
BanI G'GyrC_C

Cuts at:    744    1158    1406    1441     744 
   Size:        414     248      35    3031

  Fragments arranged by size: 

                3031     414     248      35
 
BanII G_rGCy'C

Cuts at:    290     742    1772     290 
   Size:        452    1030    2246

  Fragments arranged by size: 

                2246    1030     452
 
BbeI G_GCGC'C

Cuts at:   1162    1410    1162 
   Size:        248    3480
 
BciVI GTATCCnnnnn_n'

Cuts at:   1626    2387    3728    1626 
   Size:        761    1341    1626

  Fragments arranged by size: 

                1626    1341     761
 
BclI T'GATC_A

Cuts at:    500    1248     500 
   Size:        748    2980
 
BglII A'GATC_T

Cuts at:   1243    3280    1243 
   Size:       2037    1691
 
BlpI GC'TnA_GC

Cuts at:    840     840 
   Size:       3728
 
Bme1580I G_kGCm'C

Cuts at:    453    1353    1446    2502     453 
   Size:        900      93    1056    1679

  Fragments arranged by size: 

                1679    1056     900      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    940    1021    1356    3500     940 
   Size:         81     335    2144    1168

  Fragments arranged by size: 

                2144    1168     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    195    2095     195 
   Size:       1900    1828
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    304     904    1783    2275    2573     304 
   Size:        600     879     492     298    1459

  Fragments arranged by size: 

                1459     879     600     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3432    3432 
   Size:       3728
 
BsaAI yAC'GTr

Cuts at:   1081    1081 
   Size:       3728
 
BsaBI GATnn'nnATC

Cuts at:      6    1242    3279       6 
   Size:       1236    2037     455

  Fragments arranged by size: 

                2037    1236     455
 
BsaHI Gr'CG_yC

Cuts at:   1159    1407    2109    3605    1159 
   Size:        248     702    1496    1282

  Fragments arranged by size: 

                1496    1282     702     248
 
BsaWI w'CCGG_w

Cuts at:    917    1438    2116    2390    2537    2948     917 
   Size:        521     678     274     147     411    1697

  Fragments arranged by size: 

                1697     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1153    1911    2488    2984    1153 
   Size:        758     577     496    1897

  Fragments arranged by size: 

                1897     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    265     265 
   Size:       3728
 
BsiEI CG_ry'CG

Cuts at:    104    1270    1316    2524     104 
   Size:       1166      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1166      46
 
BsiHKAI G_wGCw'C

Cuts at:    290     453     742    1520    1710    2502     290 
   Size:        163     289     778     190     792    1516

  Fragments arranged by size: 

                1516     792     778     289     190     163
 
Bsp1286I G_dGCh'C

Cuts at:    290     453     742    1353    1446    1520    1710    1772    2502 
   Size:        163     289     611      93      74     190      62     730

Cuts at:   2502     290 
   Size:       1516

  Fragments arranged by size: 

                1516     730     611     289     190     163      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   2948    2948 
   Size:       3728
 
BspHI T'CATG_A

Cuts at:   2904    2904 
   Size:       3728
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    778     992    1294    1675     778 
   Size:        214     302     381    2831

  Fragments arranged by size: 

                2831     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2020    2074    3558    3723      50 
   Size:       1970      54    1484     165      55

  Fragments arranged by size: 

                1970    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1640    1640 
   Size:       3728
 
BsrFI r'CCGG_y

Cuts at:   1726    1907    2116    1726 
   Size:        181     209    3338

  Fragments arranged by size: 

                3338     209     181
 
BssSI C'ACGA_G

Cuts at:   1999    2357    1999 
   Size:        358    3370
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1105    1105 
   Size:       3728
 
BstBI TT'CG_AA

Cuts at:   2089    2089 
   Size:       3728
 
BstYI r'GATC_y

Cuts at:      1     753    1220    1243    1578    1824    2836    2922    2934 
   Size:        752     467      23     335     246    1012      86      12

Cuts at:   2934    3280       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     752     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3728
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1678    1859    2022    1678 
   Size:        181     163    3384

  Fragments arranged by size: 

                3384     181     163
 
BtsI GCAGTG_nn'

Cuts at:    971     971 
   Size:       3728
 
DraI TTT'AAA

Cuts at:    727     727 
   Size:       3728
 
DrdI GACnn_nn'nnGTC

Cuts at:   1434    2292    1434 
   Size:        858    2870
 
EaeI y'GGCC_r

Cuts at:    167    1313    1487    1878    1905    3250     167 
   Size:       1146     174     391      27    1345     645

  Fragments arranged by size: 

                1345    1146     645     391     174      27
 
EagI C'GGCC_G

Cuts at:   1313    1313 
   Size:       3728
 
EarI CTCTTCn'nnn_

Cuts at:    605    1751    1961     605 
   Size:       1146     210    2372

  Fragments arranged by size: 

                2372    1146     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    640    1936    2246    2392    3538     640 
   Size:       1296     310     146    1146     830

  Fragments arranged by size: 

                1296    1146     830     310     146
 
EcoICRI GAG'CTC

Cuts at:    288     740     288 
   Size:        452    3276
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1553    1985    2732    3000    1553 
   Size:        432     747     268    2281

  Fragments arranged by size: 

                2281     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    393     867     393 
   Size:        474    3254
 
EcoRI G'AATT_C

Cuts at:    732     732 
   Size:       3728
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    670    1747     670 
   Size:       1077    2651
 
FspI TGC'GCA

Cuts at:   1509    1509 
   Size:       3728
 
HaeII r_GCGC'y

Cuts at:    115     158    1162    1410    2432    3411     115 
   Size:         43    1004     248    1022     979     432

  Fragments arranged by size: 

                1022    1004     979     432     248      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1570    1600    1602    1632    1570 
   Size:         30       2      30    3666

  Fragments arranged by size: 

                3666      30      30       2
 
HincII GTy'rAC

Cuts at:    767     767 
   Size:       3728
 
KasI G'GCGC_C

Cuts at:   1158    1406    1158 
   Size:        248    3480
 
KpnI G_GTAC'C

Cuts at:    748     748 
   Size:       3728
 
MluI A'CGCG_T

Cuts at:   3161    3161 
   Size:       3728
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    521    2399    2583     521 
   Size:       1878     184    1666

  Fragments arranged by size: 

                1878    1666     184
 
MscI TGG'CCA

Cuts at:   1489    1489 
   Size:       3728
 
MslI CAynn'nnrTG

Cuts at:   1717    1844    1717 
   Size:        127    3601
 
MspA1I CmG'CkG

Cuts at:    839    1153    1513    2526    2771     839 
   Size:        314     360    1013     245    1796

  Fragments arranged by size: 

                1796    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1909    1909 
   Size:       3728
 
NarI GG'CG_CC

Cuts at:   1159    1407    1159 
   Size:        248    3480
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3728
 
NgoMIV G'CCGG_C

Cuts at:   1907    1907 
   Size:       3728
 
NspI r_CATG'y

Cuts at:    781    1281    2188     781 
   Size:        500     907    2321

  Fragments arranged by size: 

                2321     907     500
 
PciI A'CATG_T

Cuts at:   2184    2184 
   Size:       3728
 
PfoI T'CCnGG_A

Cuts at:    395     479     395 
   Size:         84    3644
 
PmeI GTTT'AAAC

Cuts at:    727     727 
   Size:       3728
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2867    2899    2867 
   Size:         32    3696
 
PpuMI rG'GwC_Cy

Cuts at:    393     393 
   Size:       3728
 
PspXI vC'TCGA_Gb

Cuts at:    239     239 
   Size:       3728
 
PstI C_TGCA'G

Cuts at:    119     775    1460     119 
   Size:        656     685    2387

  Fragments arranged by size: 

                2387     685     656
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3728
 
PvuII CAG'CTG

Cuts at:   1153    1513    1153 
   Size:        360    3368
 
RsrII CG'GwC_CG

Cuts at:   1923    1923 
   Size:       3728
 
SacI G_AGCT'C

Cuts at:    290     742     290 
   Size:        452    3276
 
SalI G'TCGA_C

Cuts at:    765     765 
   Size:       3728
 
SapI GCTCTTCn'nnn_

Cuts at:   1751    1961    1751 
   Size:        210    3518
 
SbfI CC_TGCA'GG

Cuts at:    775     775 
   Size:       3728
 
ScaI AGT'ACT

Cuts at:   3245    3245 
   Size:       3728
 
SfcI C'TryA_G

Cuts at:     33     115     771    1456    2449    2640      33 
   Size:         82     656     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     656     191      82
 
SfoI GGC'GCC

Cuts at:   1160    1408    1160 
   Size:        248    3480
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3728
 
SmaI CCC'GGG

Cuts at:    127     750     127 
   Size:        623    3105
 
SmlI C'TyrA_G

Cuts at:    239     283     883    1798    2290    2552     239 
   Size:         44     600     915     492     262    1415

  Fragments arranged by size: 

                1415     915     600     492     262      44
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3728
 
SphI G_CATG'C

Cuts at:    781    1281     781 
   Size:        500    3228
 
SspI AAT'ATT

Cuts at:    378     378 
   Size:       3728
 
StyI C'CwwG_G

Cuts at:    107     862    1216     107 
   Size:        755     354    2619

  Fragments arranged by size: 

                2619     755     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    468    1311    1444    1903    2112     468 
   Size:        843     133     459     209    2084

  Fragments arranged by size: 

                2084     843     459     209     133
 
TatI w'GTAC_w

Cuts at:   3243    3243 
   Size:       3728
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3245    3277    3245 
   Size:         32    3696
 
Tth111I GACn'n_nGTC

Cuts at:   1525    1525 
   Size:       3728
 
XbaI T'CTAG_A

Cuts at:    759     759 
   Size:       3728
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1223    1223 
   Size:       3728
 
XhoI C'TCGA_G

Cuts at:    239     239 
   Size:       3728
 
XmaI C'CCGG_G

Cuts at:    125     748     125 
   Size:        623    3105
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI    PpuMI    PspXI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI    BsiWI
     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PmlI    PshAI     PsiI   PspOMI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI
     XmnI     ZraI
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