Restriction map for pF1KB6850
##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        AocI        Aor51HI     ApaI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DdeI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoT22I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
ItaI        KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SwaI        TaqI        TseI        
Tth111I     TthHB8I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AciI          126  CCGC                 CCGC(-3/-1)
AluI           57  AGCT                 AG^CT
Alw44I        103  GTGCAC               G^TGCAC
AlwNI         515  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         103  GTGCAC               G^TGCAC
BamHI         396  GGATCC               G^GATCC
BanI           51  GGTGCC               G^G[CT][GA]CC
BciVI         173  GTATCC               GTATCC(6/5)
BglII         489  AGATCT               A^GATCT
BpmI          512  CTCCAG               CTGGAG(16/14)
BseRI         145  GAGGAG               GAGGAG(10/8)
Bsp1407I      111  TGTACA               T^GTACA
BsrGI         111  TGTACA               T^GTACA
BssKI         229  CCAGG                ^CC[ACGT]GG
BstNI         229  CCAGG                CC^[AT]GG
Cac8I          54  GCCAGC               GC[ACGT]^[ACGT]GC
DraI          264  TTTAAA               TTT^AAA
EcoNI          42  CCTGACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        229  CCAGG                ^CC[AT]GG
HphI          272  TCACC                GGTGA(8/7)
MvaI          229  CCAGG                CC^[AT]GG
MwoI          315  GCAACCACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII         24  CATG                 CATG^
PflMI          93  CCAATTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          340  GACTC                GAGTC(4/5)
ScrFI         229  CCAGG                CC^[ACGT]GG
SmlI          496  CTTGAG               C^T[CT][GA]AG
SspBI         111  TGTACA               T^GTACA
SspI          199  AATATT               AAT^ATT
StuI          231  AGGCCT               AGG^CCT
TfiI          565  GAATC                G^A[AT]TC
Van91I         93  CCAATTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwI          397  GATCC                GGATC(4/5)
              396  GGATC               
AspHI         103  GTGCAC               G[AT]GC[AT]^C
              290  GAGCAC              
BfaI          235  CTAG                 C^TAG
              481  CTAG                
BmyI          103  GTGCAC               G[AGT]GC[ACT]^C
              290  GAGCAC              
BsiHKAI       103  GTGCAC               G[AT]GC[AT]^C
              290  GAGCAC              
Bsp1286I      103  GTGCAC               G[AGT]GC[ACT]^C
              290  GAGCAC              
BstF5I        295  CATCC                GGATG(2/0)
                3  GGATG               
BstYI         396  GGATCC               [GA]^GATC[CT]
              489  AGATCT              
DpnII         397  GATC                 ^GATC
              490  GATC                
EcoRV         413  GATATC               GAT^ATC
              468  GATATC              
EcoT14I        47  CCAAGG               C^C[AT][AT]GG
              392  CCAAGG              
FokI          295  CATCC                GGATG(9/13)
                3  GGATG               
HaeIII        232  GGCC                 GG^CC
              503  GGCC                
HinfI         340  GACTC                G^A[ACGT]TC
              565  GAATC               
MaeI          235  CTAG                 C^TAG
              481  CTAG                
MboI          397  GATC                 ^GATC
              490  GATC                
MflI          396  GGATCC               [GA]^GATC[CT]
              489  AGATCT              
MseI          265  TTAA                 T^TAA
              382  TTAA                
NdeII         397  GATC                 ^GATC
              490  GATC                
NlaIV          51  GGTGCC               GG[ACGT]^[ACGT]CC
              396  GGATCC              
Sau3AI        397  GATC                 ^GATC
              490  GATC                
SfaNI         208  GATGC                GCATC(5/9)
              508  GATGC               
StyI           47  CCAAGG               C^C[AT][AT]GG
              392  CCAAGG              
Tru9I         265  TTAA                 T^TAA
              382  TTAA                
Tsp509I        95  AATT                 ^AATT
              183  AATT                
XhoII         396  GGATCC               [GA]^GATC[CT]
              489  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AfaI        BsaJI       BsiYI       BslI        BsrI        
MaeIII      MboII       MnlI        RsaI        SfcI        
Tsp45I      TspRI       
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