Restriction map for pF1KB6911
 (Circular) (Six-base) MAPSORT of: pF1KB6911.seq  Check: 7856  from: 1  to: 3728



pF1KB6911  3728 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    538     538 
   Size:       3728
 
AatII G_ACGT'C

Cuts at:    287     287 
   Size:       3728
 
AccI GT'mk_AC

Cuts at:    766     766 
   Size:       3728
 
Acc65I G'GTAC_C

Cuts at:    744     744 
   Size:       3728
 
AclI AA'CG_TT

Cuts at:   3568    3568 
   Size:       3728
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    727    1553    1985    2732    3000     727 
   Size:        826     432     747     268    1455

  Fragments arranged by size: 

                1455     826     747     432     268
 
AflIII A'CryG_T

Cuts at:   1080    2184    3161    1080 
   Size:       1104     977    1647

  Fragments arranged by size: 

                1647    1104     977
 
AgeI A'CCGG_T

Cuts at:   2116    2116 
   Size:       3728
 
AlwNI CAG_nnn'CTG

Cuts at:   1033    2600    1033 
   Size:       1567    2161
 
ApaLI G'TGCA_C

Cuts at:   2498    2498 
   Size:       3728
 
ApoI r'AATT_y

Cuts at:    732    3081    3337     732 
   Size:       2349     256    1123

  Fragments arranged by size: 

                2349    1123     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3728
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3728
 
AvaI C'yCGr_G

Cuts at:    748     748 
   Size:       3728
 
BamHI G'GATC_C

Cuts at:    194     582     753     194 
   Size:        388     171    3169

  Fragments arranged by size: 

                3169     388     171
 
BanI G'GyrC_C

Cuts at:    526     744    1158    1406    1441     526 
   Size:        218     414     248      35    2813

  Fragments arranged by size: 

                2813     414     248     218      35
 
BanII G_rGCy'C

Cuts at:    275     575     742    1772     275 
   Size:        300     167    1030    2231

  Fragments arranged by size: 

                2231    1030     300     167
 
BbeI G_GCGC'C

Cuts at:   1162    1410    1162 
   Size:        248    3480
 
BbsI GAAGACnn'nnnn_

Cuts at:    457     457 
   Size:       3728
 
BciVI GTATCCnnnnn_n'

Cuts at:   1626    2387    3728    1626 
   Size:        761    1341    1626

  Fragments arranged by size: 

                1626    1341     761
 
BclI T'GATC_A

Cuts at:   1248    1248 
   Size:       3728
 
BglI GCCn_nnn'nGGC

Cuts at:    142     142 
   Size:       3728
 
BglII A'GATC_T

Cuts at:    230    1243    3280     230 
   Size:       1013    2037     678

  Fragments arranged by size: 

                2037    1013     678
 
BlpI GC'TnA_GC

Cuts at:    840     840 
   Size:       3728
 
Bme1580I G_kGCm'C

Cuts at:   1353    1446    2502    1353 
   Size:         93    1056    2579

  Fragments arranged by size: 

                2579    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    940    1021    1356    3500     940 
   Size:         81     335    2144    1168

  Fragments arranged by size: 

                2144    1168     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2095    2095 
   Size:       3728
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    292     904    1783    2275    2573     292 
   Size:        612     879     492     298    1447

  Fragments arranged by size: 

                1447     879     612     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3432    3432 
   Size:       3728
 
BsaAI yAC'GTr

Cuts at:   1081    1081 
   Size:       3728
 
BsaBI GATnn'nnATC

Cuts at:      6     587    1242    3279       6 
   Size:        581     655    2037     455

  Fragments arranged by size: 

                2037     655     581     455
 
BsaHI Gr'CG_yC

Cuts at:    284    1159    1407    2109    3605     284 
   Size:        875     248     702    1496     407

  Fragments arranged by size: 

                1496     875     702     407     248
 
BsaWI w'CCGG_w

Cuts at:    585     917    1438    2116    2390    2537    2948     585 
   Size:        332     521     678     274     147     411    1365

  Fragments arranged by size: 

                1365     678     521     411     332     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    122     152     650     680     122 
   Size:         30     498      30    3170

  Fragments arranged by size: 

                3170     498      30      30
 
BseYI C'CCAG_C

Cuts at:    299    1153    1911    2488    2984     299 
   Size:        854     758     577     496    1043

  Fragments arranged by size: 

                1043     854     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1270    1316    2524     104 
   Size:       1166      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1166      46
 
BsiHKAI G_wGCw'C

Cuts at:    742    1520    1710    2502     742 
   Size:        778     190     792    1968

  Fragments arranged by size: 

                1968     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    630     630 
   Size:       3728
 
Bsp1286I G_dGCh'C

Cuts at:    275     575     742    1353    1446    1520    1710    1772    2502 
   Size:        300     167     611      93      74     190      62     730

Cuts at:   2502     275 
   Size:       1501

  Fragments arranged by size: 

                1501     730     611     300     190     167      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:    585    2948     585 
   Size:       2363    1365
 
BspHI T'CATG_A

Cuts at:    368    2904     368 
   Size:       2536    1192
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    538     778     992    1294    1675     538 
   Size:        240     214     302     381    2591

  Fragments arranged by size: 

                2591     381     302     240     214
 
BsrBI CCG'CTC

Cuts at:     50    2020    2074    3558    3723      50 
   Size:       1970      54    1484     165      55

  Fragments arranged by size: 

                1970    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    431    1640     431 
   Size:       1209    2519
 
BsrFI r'CCGG_y

Cuts at:    248    1726    1907    2116     248 
   Size:       1478     181     209    1860

  Fragments arranged by size: 

                1860    1478     209     181
 
BsrGI T'GTAC_A

Cuts at:    254     254 
   Size:       3728
 
BssSI C'ACGA_G

Cuts at:   1999    2357    1999 
   Size:        358    3370
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1105    1105 
   Size:       3728
 
BstBI TT'CG_AA

Cuts at:   2089    2089 
   Size:       3728
 
BstEII G'GTnAC_C

Cuts at:    210     244     210 
   Size:         34    3694
 
BstYI r'GATC_y

Cuts at:      1     194     230     582     753    1220    1243    1578    1824 
   Size:        193      36     352     171     467      23     335     246

Cuts at:   1824    2836    2922    2934    3280       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     467     449     352     346     335     246     193
                 171      86      36      23      12
 
BtgI C'CryG_G

Cuts at:    215     239     497     215 
   Size:         24     258    3446

  Fragments arranged by size: 

                3446     258      24
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    156    1678    1859    2022     156 
   Size:       1522     181     163    1862

  Fragments arranged by size: 

                1862    1522     181     163
 
BtsI GCAGTG_nn'

Cuts at:    650     971     650 
   Size:        321    3407
 
DraI TTT'AAA

Cuts at:    727     727 
   Size:       3728
 
DrdI GACnn_nn'nnGTC

Cuts at:    517    1434    2292     517 
   Size:        917     858    1953

  Fragments arranged by size: 

                1953     917     858
 
EaeI y'GGCC_r

Cuts at:    143     167     266     380    1313    1487    1878    1905    3250 
   Size:         24      99     114     933     174     391      27    1345

Cuts at:   3250     143 
   Size:        621

  Fragments arranged by size: 

                1345     933     621     391     174     114      99      27
                  24
 
EagI C'GGCC_G

Cuts at:   1313    1313 
   Size:       3728
 
EarI CTCTTCn'nnn_

Cuts at:   1751    1961    1751 
   Size:        210    3518
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1936    2246    2392    3538    1936 
   Size:        310     146    1146    2126

  Fragments arranged by size: 

                2126    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    740     740 
   Size:       3728
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    727    1553    1985    2732    3000     727 
   Size:        826     432     747     268    1455

  Fragments arranged by size: 

                1455     826     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    867     867 
   Size:       3728
 
EcoRI G'AATT_C

Cuts at:    732     732 
   Size:       3728
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1747    1747 
   Size:       3728
 
FspI TGC'GCA

Cuts at:   1509    1509 
   Size:       3728
 
HaeII r_GCGC'y

Cuts at:    189     306    1162    1410    2432    3411     189 
   Size:        117     856     248    1022     979     506

  Fragments arranged by size: 

                1022     979     856     506     248     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    122     154     631     650     663     682    1570    1600    1602 
   Size:         32     477      19      13      19     888      30       2

Cuts at:   1602    1632     122 
   Size:         30    2218

  Fragments arranged by size: 

                2218     888     477      32      30      30      19      19
                  13       2
 
HincII GTy'rAC

Cuts at:    767     767 
   Size:       3728
 
KasI G'GCGC_C

Cuts at:   1158    1406    1158 
   Size:        248    3480
 
KpnI G_GTAC'C

Cuts at:    748     748 
   Size:       3728
 
MluI A'CGCG_T

Cuts at:   3161    3161 
   Size:       3728
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    422    2399    2583     422 
   Size:       1977     184    1567

  Fragments arranged by size: 

                1977    1567     184
 
MscI TGG'CCA

Cuts at:    145     169     382    1489     145 
   Size:         24     213    1107    2384

  Fragments arranged by size: 

                2384    1107     213      24
 
MslI CAynn'nnrTG

Cuts at:    145     373    1717    1844     145 
   Size:        228    1344     127    2029

  Fragments arranged by size: 

                2029    1344     228     127
 
MspA1I CmG'CkG

Cuts at:    839    1153    1513    2526    2771     839 
   Size:        314     360    1013     245    1796

  Fragments arranged by size: 

                1796    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1909    1909 
   Size:       3728
 
NarI GG'CG_CC

Cuts at:   1159    1407    1159 
   Size:        248    3480
 
NcoI C'CATG_G

Cuts at:    239     497     239 
   Size:        258    3470
 
NgoMIV G'CCGG_C

Cuts at:   1907    1907 
   Size:       3728
 
NspI r_CATG'y

Cuts at:    781    1281    2188     781 
   Size:        500     907    2321

  Fragments arranged by size: 

                2321     907     500
 
PciI A'CATG_T

Cuts at:   2184    2184 
   Size:       3728
 
PmeI GTTT'AAAC

Cuts at:    727     727 
   Size:       3728
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2867    2899    2867 
   Size:         32    3696
 
PstI C_TGCA'G

Cuts at:    180     775    1460     180 
   Size:        595     685    2448

  Fragments arranged by size: 

                2448     685     595
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3728
 
PvuII CAG'CTG

Cuts at:   1153    1513    1153 
   Size:        360    3368
 
RsrII CG'GwC_CG

Cuts at:   1923    1923 
   Size:       3728
 
SacI G_AGCT'C

Cuts at:    742     742 
   Size:       3728
 
SalI G'TCGA_C

Cuts at:    765     765 
   Size:       3728
 
SapI GCTCTTCn'nnn_

Cuts at:   1751    1961    1751 
   Size:        210    3518
 
SbfI CC_TGCA'GG

Cuts at:    775     775 
   Size:       3728
 
ScaI AGT'ACT

Cuts at:   3245    3245 
   Size:       3728
 
SfcI C'TryA_G

Cuts at:     33     176     771    1456    2449    2640      33 
   Size:        143     595     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     595     191     143
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    142     142 
   Size:       3728
 
SfoI GGC'GCC

Cuts at:   1160    1408    1160 
   Size:        248    3480
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3728
 
SmaI CCC'GGG

Cuts at:    750     750 
   Size:       3728
 
SmlI C'TyrA_G

Cuts at:    307     883    1798    2290    2552     307 
   Size:        576     915     492     262    1483

  Fragments arranged by size: 

                1483     915     576     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3728
 
SphI G_CATG'C

Cuts at:    781    1281     781 
   Size:        500    3228
 
StyI C'CwwG_G

Cuts at:    239     497     862    1216     239 
   Size:        258     365     354    2751

  Fragments arranged by size: 

                2751     365     354     258
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    232     250    1311    1444    1903    2112     232 
   Size:         18    1061     133     459     209    1848

  Fragments arranged by size: 

                1848    1061     459     209     133      18
 
TatI w'GTAC_w

Cuts at:    254    3243     254 
   Size:       2989     739
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    147     179    3245    3277     147 
   Size:         32    3066      32     598

  Fragments arranged by size: 

                3066     598      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1525    1525 
   Size:       3728
 
XbaI T'CTAG_A

Cuts at:    477     759     477 
   Size:        282    3446
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1223    1223 
   Size:       3728
 
XmaI C'CCGG_G

Cuts at:    748     748 
   Size:       3728
 
ZraI GAC'GTC

Cuts at:    285     285 
   Size:       3728
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI     BpmI
   Bpu10I    BseRI     BsgI    BsiWI    BsmBI   BssHII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI
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