Restriction map for pF1KB6911
 (Linear) (Six-base) MAPSORT of: KIBB6911.seq  Check: 3528  from: 1  to: 615



KIBB6911.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     430     615 
   Size:        430     185
 
AatII G_ACGT'C

Cuts at:      0     179     615 
   Size:        179     436
 
BamHI G'GATC_C

Cuts at:      0      86     474     615 
   Size:         86     388     141

  Fragments arranged by size: 

                 388     141      86
 
BanI G'GyrC_C

Cuts at:      0     418     615 
   Size:        418     197
 
BanII G_rGCy'C

Cuts at:      0     167     467     615 
   Size:        167     300     148

  Fragments arranged by size: 

                 300     167     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     349     615 
   Size:        349     266
 
BglI GCCn_nnn'nGGC

Cuts at:      0      34     615 
   Size:         34     581
 
BglII A'GATC_T

Cuts at:      0     122     615 
   Size:        122     493
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     615 
   Size:        184     431
 
BsaBI GATnn'nnATC

Cuts at:      0     479     615 
   Size:        479     136
 
BsaHI Gr'CG_yC

Cuts at:      0     176     615 
   Size:        176     439
 
BsaWI w'CCGG_w

Cuts at:      0     477     615 
   Size:        477     138
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      14      44     542     572     615 
   Size:         14      30     498      30      43

  Fragments arranged by size: 

                 498      43      30      30      14
 
BseYI C'CCAG_C

Cuts at:      0     191     615 
   Size:        191     424
 
BsmI GAATG_Cn'

Cuts at:      0     522     615 
   Size:        522      93
 
Bsp1286I G_dGCh'C

Cuts at:      0     167     467     615 
   Size:        167     300     148

  Fragments arranged by size: 

                 300     167     148
 
BspEI T'CCGG_A

Cuts at:      0     477     615 
   Size:        477     138
 
BspHI T'CATG_A

Cuts at:      0     260     615 
   Size:        260     355
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     430     615 
   Size:        430     185
 
BsrDI GCAATG_nn'

Cuts at:      0     323     615 
   Size:        323     292
 
BsrFI r'CCGG_y

Cuts at:      0     140     615 
   Size:        140     475
 
BsrGI T'GTAC_A

Cuts at:      0     146     615 
   Size:        146     469
 
BstEII G'GTnAC_C

Cuts at:      0     102     136     615 
   Size:        102      34     479

  Fragments arranged by size: 

                 479     102      34
 
BstYI r'GATC_y

Cuts at:      0      86     122     474     615 
   Size:         86      36     352     141

  Fragments arranged by size: 

                 352     141      86      36
 
BtgI C'CryG_G

Cuts at:      0     107     131     389     615 
   Size:        107      24     258     226

  Fragments arranged by size: 

                 258     226     107      24
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      48     615 
   Size:         48     567
 
BtsI GCAGTG_nn'

Cuts at:      0     542     615 
   Size:        542      73
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     409     615 
   Size:        409     206
 
EaeI y'GGCC_r

Cuts at:      0      35      59     158     272     615 
   Size:         35      24      99     114     343

  Fragments arranged by size: 

                 343     114      99      35      24
 
HaeII r_GCGC'y

Cuts at:      0      81     198     615 
   Size:         81     117     417

  Fragments arranged by size: 

                 417     117      81
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      14      46     523     542     555     574     615 
   Size:         14      32     477      19      13      19      41

  Fragments arranged by size: 

                 477      41      32      19      19      14      13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     615 
   Size:        314     301
 
MscI TGG'CCA

Cuts at:      0      37      61     274     615 
   Size:         37      24     213     341

  Fragments arranged by size: 

                 341     213      37      24
 
MslI CAynn'nnrTG

Cuts at:      0      37     265     615 
   Size:         37     228     350

  Fragments arranged by size: 

                 350     228      37
 
NcoI C'CATG_G

Cuts at:      0     131     389     615 
   Size:        131     258     226

  Fragments arranged by size: 

                 258     226     131
 
PstI C_TGCA'G

Cuts at:      0      72     615 
   Size:         72     543
 
SfcI C'TryA_G

Cuts at:      0      68     615 
   Size:         68     547
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      34     615 
   Size:         34     581
 
SmlI C'TyrA_G

Cuts at:      0     199     615 
   Size:        199     416
 
StyI C'CwwG_G

Cuts at:      0     131     389     615 
   Size:        131     258     226

  Fragments arranged by size: 

                 258     226     131
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     124     142     615 
   Size:        124      18     473

  Fragments arranged by size: 

                 473     124      18
 
TatI w'GTAC_w

Cuts at:      0     146     615 
   Size:        146     469
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      39      71     615 
   Size:         39      32     544

  Fragments arranged by size: 

                 544      39      32
 
XbaI T'CTAG_A

Cuts at:      0     369     615 
   Size:        369     246
 
ZraI GAC'GTC

Cuts at:      0     177     615 
   Size:        177     438
 

 Enzymes that do cut:

     AarI    AatII    BamHI     BanI    BanII     BbsI     BglI    BglII
    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BstEII    BstYI
     BtgI    BtgZI     BtsI     DrdI     EaeI    HaeII    Hin4I     MmeI
     MscI     MslI     NcoI     PstI     SfcI     SfiI     SmlI     StyI
    TaqII     TatI     TstI     XbaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BsrBI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XcmI     XhoI     XmaI     XmnI
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