Restriction map for pF1KB8253
 (Linear) (Six-base) MAPSORT of: KIBB8253.seq  Check: 205  from: 1  to: 228



KIBB8253.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

ApoI r'AATT_y

Cuts at:      0     102     228 
   Size:        102     126
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     172     228 
   Size:        172      56
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     228 
   Size:         40     188
 
BtsI GCAGTG_nn'

Cuts at:      0      24     228 
   Size:         24     204
 
EcoRI G'AATT_C

Cuts at:      0     102     228 
   Size:        102     126
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      36      68     228 
   Size:         36      32     160

  Fragments arranged by size: 

                 160      36      32
 
SmlI C'TyrA_G

Cuts at:      0      55     228 
   Size:         55     173
 

 Enzymes that do cut:

     ApoI     BmrI    BpuEI     BtsI    EcoRI    Hin4I     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com